Task Response When we exercise, our muscles use energy (ATP). Most of this energy is produced from aerobic respiration, but anaerobic respiration can produce energy faster. Anaerobic respiration is most common during high-intensity exercise. It is less efficient, but can provide more energy when muscles need it 1 quickly. The process produces a useless by-product, lactic acid, which can build up in muscles and is associated with muscle fatigue. When a muscle is unable to contract with the same force, muscle fatigue is occurring. In this part of the activity, you will observe how high-intensity exercise can lead to muscle fatigue, which can be used as a sign that anaerobic respiration has occurred in the body. Obtain a clothespin and a timer. Decide who will be the test 2 subject for this investigation. Table 2. Number of Clothespin Have the test subject grasp the clothespin between the thumb Squeezes 3 and forefinger of the dominant hand. Trial # # of Squeezes Difference 4 When ready, start the timer and say “GO!” from Trial 1 The test subject should start squeezing the clothespin open 1 36 N/A and closed as fast as possible for 15 seconds, counting the 2 35 1 5 number of squeezes. 3 30 6 At 15 seconds have the test subject stop squeezing the 4 33 3 6 clothespin, and QUICKLY record the number of squeezes in 5 31 5 Table 2 for Trial 1. 6 29 7 7 The test subject should REST ONLY 5 SECONDS! 7 28 8 After 5 seconds, have the test subject squeeze the clothespin 8 26 10 again (same hand) for 15 seconds. Record the number of 8 9 26 10 squeezes in Table 2, Trial 2. 10 27 9 Continue with a 15-second test and 5-second rest interval for 11 24 12 9 a total of 15 trials. Record the results in Table 2. No 12 25 11 switching fingers or hands! 13 27 9 When finished, subtract the number of squeezes for each trial 14 25 11 10 from trial 1. Record the values in Table 2. 15 23 13 How does muscle fatigue indicate Answer: 11 that anaerobic respiration has Lactic acid is a product of anaerobic metabolism, in which the body produces energy without using oxygen. Since lactic acid is produced, this can build up in occurred? muscles which then causes pain, fatigue and the decrease in oxygen What happened to the number of Answer: clothespin squeezes as the number As the number of trials increased, the number of clothespin squeezes 12 of trials increased? Hypothesize decreased slowly after every trial. This is because of the muscle why this happened. fatigue in the hand after the number of times that the hand has squeezed the clothespin Construct an explanation based on Answer: the evidence from this lab for the Based on the evidence collected, it shows that the number of squeezes 13 cycling of matter and flow of started to decrease slowly which means that the flow of oxygen and energy in anaerobic conditions. energy wasn’t being delivered fast enough. This initiates the process of turning glucose into lactid acid, and this makes the muscles start to fatigue and