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  • Perhitungan Vitamin

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    Maritsya Dita Kurnia Putri
    12 views6 pages
    The document provides details on the calculation of Vitamins B1, B3, and B6 levels using ESTD and ISTD methods. It includes retention times, concentration levels, and area measurements for 10 standard samples of each vitamin. It also provides linear regression equations to determine vitamin concentrations based on area. The procedure for the ISTD method involves analyzing standards and samples by GC and using the internal standard nonivamid to calculate concentration ratios.

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    The document provides details on the calculation of Vitamins B1, B3, and B6 levels using ESTD and ISTD methods. It includes retention times, concentration levels, and area measurements for 10 standard samples of each vitamin. It also provides linear regression equations to determine vitamin concentrations based on area. The procedure for the ISTD method involves analyzing standards and samples by GC and using the internal standard nonivamid to calculate concentration ratios.

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    © All Rights Reserved
    Download as XLS, PDF, TXT or read online from Scribd
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    12 views6 pages

    Perhitungan Vitamin

    Uploaded by

    Maritsya Dita Kurnia Putri
    The document provides details on the calculation of Vitamins B1, B3, and B6 levels using ESTD and ISTD methods. It includes retention times, concentration levels, and area measurements for 10 standard samples of each vitamin. It also provides linear regression equations to determine vitamin concentrations based on area. The procedure for the ISTD method involves analyzing standards and samples by GC and using the internal standard nonivamid to calculate concentration ratios.

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    © All Rights Reserved
    Download as XLS, PDF, TXT or read online from Scribd
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    of 6

    Component Summary: ESTD Sunday, May 11, 2014 12:53:17 PM

    REKAPITULASI PERHITUNGAN DENGAN ESTD

    VITAMIN B1, B3, B6

    RT konsentrasi AREA
    NO NAMA STANDARD
    (Menit) (ppb) Vitamin B1 Vitamin B3 Vitamin B6

    1 Standard_VITB_1_inj2 0.48 75.00 35,243.14 66,253.38 35,052.03

    2 Standard_VITB_1_inj3 0.46 75.00 35,489.77 66,583.87 35,221.15

    3 Standard_VITB_2_inj4 0.48 200.00 87,115.73 146,111.75 75,825.09

    4 Standard_VITB_2_inj5 0.47 200.00 86,885.86 144,479.55 74,173.29

    5 Standard_VITB_3_inj2 0.47 300.00 122,755.81 213,780.01 98,366.77

    6 Standard_VITB_3_inj3 0.46 300.00 121,632.15 214,661.65 97,785.06

    7 Standard_VITB_4_inj2 0.47 400.00 156,120.00 313,591.63 139,885.65

    8 Standard_VITB_4_inj4 0.47 400.00 155,500.00 312,529.00 140,549.14

    9 Standard_VITB_5_inj4 0.47 500.00 186,872.37 390,027.56 161,838.87

    10 Standard_VITB_5_inj5 0.48 500.00 187,476.95 391,336.26 162,889.71

    450,000.00

    400,000.00

    f(x) = 774.814879146328 x − 2634.9241158363


    R² = 0.992786475180809
    350,000.00

    300,000.00

    250,000.00

    200,000.00
    f(x) = 355.621090970331 x + 12600.9559719826
    R² = 0.996692975004079
    150,000.00 f(x) = 304.460733656282 x + 12342.7580650447
    R² = 0.992475022553743

    100,000.00

    50,000.00

    -
    50.00
    Vit_B1 100.00 150.00 200.00
    Linear (Vit_B1)250.00 Vit
    300.00
    B3 350.00 400.00
    Linear450.00
    (Vit B3) 500.00 Vit_B6
    550.00
    Linear (Vit_B6) Linear (Vit_B6) Linear (Vit_B6)

    Page 1 of 6
    Component Summary: ESTD Sunday, May 11, 2014 12:53:17 PM

    REKAPITULASI PERHITUNGAN DENGAN ESTD

    VITAMIN B1, B3, B6

    RT konsentrasi AREA
    NO NAMA STANDARD
    (Menit) (ppb) Vitamin B1 Vitamin B3 Vitamin B6
    LUAS AREA
    VIT LUAS AREA PPB PPM VIAL
    TERHITUNG
    B3 5,225,872.00 1,088,723.33 1,408.55 1.41 2.82
    B1 287,387.00 59,872.29 80.67 0.08 0.16
    B6 1,507,127.00 313,984.79 408.64 0.41 0.82

    Page 2 of 6
    Component Summary: ISTD Sunday, May 11, 2014 12:53:17 PM

    REKAPITULASI PERHITUNGAN DENGAN ISTD

    VITAMIN B1, B3, B6 DENGAN NONIVAMID 500 PPB

    RT konsentrasi AREA konsentrasi


    NO NAMA STANDARD
    (Menit) (ppb) Vitamin B1 Vitamin B3 Vitamin B6 Nonivamid Rasio

    1 Standard_VITB_1_inj2 0.48 75.00 35,243.14 66,253.38 35,052.03 283,962.39 0.150

    2 Standard_VITB_1_inj3 0.46 75.00 35,489.77 66,583.87 35,221.15 284,851.88 0.150

    3 Standard_VITB_2_inj4 0.48 200.00 87,115.73 146,111.75 75,825.09 285,012.00 0.400

    4 Standard_VITB_2_inj5 0.47 200.00 86,885.86 144,479.55 74,173.29 286,254.00 0.400

    5 Standard_VITB_3_inj2 0.47 300.00 122,755.81 213,780.01 98,366.77 283,012.00 0.600

    6 Standard_VITB_3_inj3 0.46 300.00 121,632.15 214,661.65 97,785.06 284,025.00 0.600

    7 Standard_VITB_4_inj2 0.47 400.00 156,120.00 313,591.63 139,885.65 290,613.29 0.800

    8 Standard_VITB_4_inj4 0.47 400.00 155,500.00 312,529.00 140,549.14 290,385.99 0.800

    9 Standard_VITB_5_inj4 0.47 500.00 186,872.37 390,027.56 161,838.87 291,465.49 1.000

    10 Standard_VITB_5_inj5 0.48 500.00 187,476.95 391,336.26 162,889.71 291,830.07 1.000

    1.600

    1.400

    f(x) = 1.32054657769551 x + 0.00388322025203447


    R² = 0.995164429638635
    1.200

    1.000

    0.800
    Page 3 of 6
    f(x) = 0.626176385921863 x + 0.0443754393091096
    0.600 R² = 0.996692975004079
    f(x) = 0.51770276878473 x + 0.0488432708283507
    1.600
    Component Summary: ISTD Sunday, May 11, 2014 12:53:17 PM

    1.400
    REKAPITULASI PERHITUNGAN DENGAN ISTD

    VITAMIN B1, B3, B6f(x) = 1.32054657769551


    DENGAN NONIVAMIDx 500
    + 0.00388322025203447
    PPB
    R² = 0.995164429638635
    1.200

    RT konsentrasi AREA konsentrasi


    NO NAMA STANDARD
    1.000 (Menit) (ppb) Vitamin B1 Vitamin B3 Vitamin B6 Nonivamid Rasio

    0.800

    f(x) = 0.626176385921863 x + 0.0443754393091096


    0.600 R² = 0.996692975004079
    f(x) = 0.51770276878473 x + 0.0488432708283507
    R² = 0.993804189427008
    0.400

    0.200

    -
    0.100 0.200 0.300 0.400 0.500 0.600 0.700 0.800 0.900 1.000 1.100
    Vit B1 Linear (Vit B1) Vit B3 Linear (Vit B3) Vit B6 Linear (Vit B6)

    Page 4 of 6
    Component Summary: ISTD Sunday, May 11, 2014 12:53:17 PM

    REKAPITULASI PERHITUNGAN DENGAN ISTD

    VITAMIN B1, B3, B6 DENGAN NONIVAMID 500 PPB

    AREA
    NO
    Vitamin B1 Vitamin B3 Vitamin B6

    1 0.124 0.233 0.123

    2 0.125 0.234 0.124

    3 0.307 0.513 0.266

    4 0.306 0.505 0.259

    5 0.432 0.755 0.348

    6 0.428 0.756 0.344

    7 0.550 1.079 0.481

    8 0.548 1.076 0.484

    9 0.658 1.338 0.555

    10 0.660 1.341 0.558

    1.600

    1.400

    f(x) = 1.32054657769551 x + 0.00388322025203447


    R² = 0.995164429638635
    1.200

    1.000

    0.800
    Page 5 of 6
    f(x) = 0.626176385921863 x + 0.0443754393091096
    0.600 R² = 0.996692975004079
    f(x) = 0.51770276878473 x + 0.0488432708283507
    PROSEDUR ISTD

    A STANDARD VALIDASI
    1 DITIMBANG STANDARD FAME DENGAN BERBAGAI KONSENTRASI
    2 DITIMBANG BENZYL ALCOHOL SEBAGAI PEMBANDING
    3 DIANALISIS DENGAN GC
    4 DIANALISIS HASIL GC UNTUK MENENTUKAN VALIDASI FAME
    5 DIDAPATKAN PERSAMAAN DAN KOEFISIEN KORELASINYA

    B PENENTUAN SAMPEL FAME


    1 DITIMBANG SAMPEL FAME (Gr)
    2 DITIMBANG BENZYL ALCOHOL SEBAGAI PEMBANDING (Gr)
    3 DIANALISIS DENGAN GC
    4 DIANALISIS HASIL GC UNTUK MENENTUKAN KADAR (%) FAME

    C KONDISI OPERASI
    1 NAMA ALAT = HP 5890
    2 KOLOM = OV-17
    3 GAS PEMBAWA = NITROGEN
    4 KECEPATAN = 28 ML/MENIT
    5 INITIAL TEMP = 125
    6 INITIAL TIME = 3 MENIT
    7 RATE = 15 DEG/MENIT
    8 FINAL TEMP = 275
    9 DETEKTOR = FID

    D PERHITUNGAN DENGAN EXCELL


    STANDARD
    MASUKKAN HASIL AREA BENZYL ALKOHOL PADA KOLOM YANG DITENTUKAN
    MASUKKAN HASIL AREA TOTAL PADA KOLOM YANG DITENTUKAN
    KURANGI AREA TOTAL DENGAN AREA BENZYL ALKOHOL

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