QuantStudio™ Design & Analysis

Software v1.5.1
Experiment Results Report

Experiment Summary
Experiment Name: COVID-19 MERIL RUN TEMPLATE
Experiment Type: Standard Curve

Chemistry: TaqMan® Reagents

BarCode:

File Name: COVID-19 MERIL 2ND RUN 16-11-2022.eds

Run Started: 11-16-2022 05:40:34 PST

Run Finished: 11-16-2022 07:10:39 PST

Run Duration: 90 minutes 4 seconds

Date EDS Modified: 11-16-2022 15:08:01 PST

Date EDS Created: 12-14-2020 23:18:59 PST

User Name:

Number of Wells Used: 22

Number of Wells with Results: 22

Instrument Name: 272526081

Instrument Type: QuantStudio™ 5 System

Instrument Serial Number: 272526081

Model/Block Type: QuantStudio™ 5 System / 96-Well 0.2-mL Block

Quantification Cycle Setting: Cт

Stage/Step for Analysis: Stage2, Step2

Comments:

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Experiment Results Report

Reagent Information
Type Name Part Number Lot Number Expiration Date

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Experiment Results Report

Results Summary
Sample Target Qty Mean Qty SD Cт Mean Cт SD

10172897 IC 0.000 25.132

10177425 IC 0.000 27.287

10177564 IC 0.000 25.657

10177566 IC 0.000 27.180

10177641 IC 0.000 27.311

10178748 IC 0.000 27.752

10178749 IC 0.000 25.532

10178751 IC 0.000 25.875

10178752 IC 0.000 26.337

10178753 IC 0.000 25.898

10178754 IC 0.000 26.393

10178755 IC 0.000 26.005

10178756 IC 0.000 26.420

10178758 IC 0.000 26.541

10178760 IC 0.000 26.186

10178761 IC 0.000 23.982

10178762 IC 0.000 25.977

1178756 IC 0.000 25.920

NC IC

PC IC 24.691

10172897 N GENE 0.000 20.078

10177425 N GENE 0.000 27.293

10177564 N GENE 0.000 23.441

10177566 N GENE

10177641 N GENE

10178748 N GENE

10178749 N GENE

10178751 N GENE

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Sample Target Qty Mean Qty SD Cт Mean Cт SD

10178752 N GENE

10178753 N GENE

10178754 N GENE

10178755 N GENE

10178756 N GENE

10178758 N GENE

10178760 N GENE

10178761 N GENE

10178762 N GENE

1178756 N GENE

NC N GENE

PC N GENE 24.167

10172897 ORF1ab 0.000 25.004

10177425 ORF1ab 0.000 28.012

10177564 ORF1ab 0.000 24.538

10177566 ORF1ab

10177641 ORF1ab

10178748 ORF1ab

10178749 ORF1ab

10178751 ORF1ab

10178752 ORF1ab

10178753 ORF1ab

10178754 ORF1ab

10178755 ORF1ab

10178756 ORF1ab

10178758 ORF1ab

10178760 ORF1ab

10178761 ORF1ab

10178762 ORF1ab

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Sample Target Qty Mean Qty SD Cт Mean Cт SD

1178756 ORF1ab

NC ORF1ab

PC ORF1ab 24.630

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Experiment Results Report

Plate Layout

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Experiment Results Report

Amplification Plot (ΔRn vs Cycle)

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Experiment Results Report

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Amplification Plot (Rn vs Cycle)

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Experiment Results Report

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Experiment Results Report

Amplification Plot (Cт vs Well)

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Standard Curves

Slope: 0.000 Y-Intercept: 0.000 R Square: 0.000 Efficiency: -100.000

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Slope: 0.000 Y-Intercept: 0.000 R Square: 0.000 Efficiency: -100.000

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Slope: 0.000 Y-Intercept: 0.000 R Square: 0.000 Efficiency: -100.000

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Experiment Results Report

Results Table
Cт Cт Baseline Baseline
Well Sample Target Task Cт Cт SD Qty Qty Mean Qty SD Cq Conf
Mean Threshold Start End

B1 10178760 IC U 26.186 26.186 0.000 0.000 21,368.457 3 19 0.979

B1 10178760 N GENE U UND. 6,509.215 3 7 0.000

B1 10178760 ORF1ab U UND. 30,749.809 3 39 0.000

B3 10178762 IC U 25.977 25.977 0.000 0.000 21,368.457 3 17 0.986

B3 10178762 N GENE U UND. 6,509.215 3 21 0.000

B3 10178762 ORF1ab U UND. 30,749.809 3 39 0.000

B2 10178761 IC U 23.982 23.982 0.000 0.000 21,368.457 3 13 0.990

B2 10178761 N GENE U UND. 6,509.215 3 26 0.000

B2 10178761 ORF1ab U UND. 30,749.809 3 39 0.000

B5 10172897 IC U 31.732 31.732 0.000 0.000 21,368.457 3 18 0.590

B5 10172897 N GENE U 20.078 20.078 0.000 0.000 6,509.215 3 13 0.865

B5 10172897 ORF1ab U 25.004 25.004 0.000 0.000 30,749.809 3 14 0.343

B4 10177425 IC U 27.287 27.287 0.000 0.000 21,368.457 3 19 0.990

B4 10177425 N GENE U 27.293 27.293 0.000 0.000 22,265.457 3 30 0.924

B4 10177425 ORF1ab U 28.012 28.012 0.000 0.000 25,753.180 3 32 0.000

B7 10177566 IC U 27.180 27.180 0.000 0.000 21,368.457 3 17 0.985

B7 10177566 N GENE U UND. 6,509.215 3 3 0.000

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Experiment Results Report

Cт Cт Baseline Baseline
Well Sample Target Task Cт Cт SD Qty Qty Mean Qty SD Cq Conf
Mean Threshold Start End

B7 10177566 ORF1ab U UND. 30,749.809 3 39 0.000

B6 10177564 IC U 25.657 25.657 0.000 0.000 21,368.457 3 16 0.991

B6 10177564 N GENE U 23.441 23.441 0.000 0.000 6,509.215 3 17 0.962

B6 10177564 ORF1ab U 24.538 24.538 0.000 0.000 30,749.809 3 17 0.970

B8 10177641 IC U 27.311 27.311 0.000 0.000 21,368.457 3 19 0.988

B8 10177641 N GENE U UND. 6,509.215 3 21 0.000

B8 10177641 ORF1ab U UND. 30,749.809 3 39 0.000

B11 NC IC N 26.824 21,368.457 3 17 0.981

B11 NC N GENE N UND. 6,509.215 3 16 0.000

B11 NC ORF1ab N UND. 30,749.809 3 8 0.000

B12 PC IC S 24.691 24.691 1.000 21,368.457 3 17 0.984

B12 PC N GENE S 24.167 24.167 1.000 6,509.215 3 19 0.958

B12 PC ORF1ab S 24.630 24.630 1.000 30,749.809 3 18 0.932

Legend: S = Standard, N = Non Template Control, U = Unknown, UND. = Undetermined

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Experiment Results Report

QC Summary
Total Wells:96 Processed Wells:22 Targets Used:3 Well Setup:22 Flagged Wells:20 Samples Used:20

Flag Description Frequency Locations

AMPNC Amplification in negative control 1 B11

BADROX Bad passive reference signal 0

BLFAIL Baseline algorithm failed 0

CQCONF Low Cq confidence 5 B1, B3, B2, B7, B8

CQCONF Low Cq confidence 1 B5

CQCONF Low Cq confidence 7 B1, B3, B2, B5, B4, B7, B8

CTFAIL Cт algorithm failed 0

DRNMIN Define acceptable delta Rn based on Cт range 0

EXPFAIL Exponential algorithm failed 5 B1, B3, B2, B7, B8

HIGHSD High standard deviation in replicate group 0

NOAMP No amplification 0

NOISE Noise higher than others in plate 0

NOSIGNAL No signal in well 0

OFFSCALE Fluorescence is offscale 0

OUTLIERRG Outlier in replicate group 0

PRFDROP Passive reference signal changes near Cт 0

PRFLOW Low passive reference signal 0

SPIKE Noise spikes 0

THOLDFAIL Thresholding algorithm failed 0

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TC Protocol
Block Type: 96-Well 0.2-mL Block
Run Mode: Standard

Sample Volume: 20.0

Cover Temperature: 105.0

PCR Filter Set: m1,x1 m2,x1 m2,x2 m3,x2 m3,x3 m4,x3 m4,x4 m5,x4 m5,x5 m6,x5

Melt Curve Filter Set: Not Applicable

Stage No. of Repetitions Starting Cycle Auto Delta Enabled

Hold Stage 1 1 false

Cycling Stage 40 1 false

Auto Delta Auto Delta

Stage Step Collection Flag Ramp Rate Temperature Hold Time
Temperature Hold Time

Hold Stage 1 Disabled 1.6 °C/s 50.0 900 0.0 0

Hold Stage 2 Disabled 1.6 °C/s 95.0 180 0.0 0

Cycling Stage 1 Disabled 1.6 °C/s 95.0 15 0.0 0

Cycling Stage 2 Enabled 1.6 °C/s 55.0 40 0.0 0

Customer is responsible for any validation of assays and compliance with regulatory requirements that
pertain to their procedures and uses of reagents and/or instruments.©2018 Thermo Fisher Scientific. All
rights reserved. The trademarks mentioned herein are the property of Thermo Fisher Scientific or their
respective owners.

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