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  • Cells & Extracellular Matrix (H&E) - Stained Tissues

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    Ishita guleria
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    Tissues are composed of cells and extracellular matrix. Slides used in histology labs contain formalin-fixed, paraffin-embedded tissues stained with hematoxylin and eosin. The preparation of tissues for examination under a microscope involves fixation with formalin, dehydration with ethanol, clearing with xylene, infiltration and embedding with paraffin, sectioning, staining with hematoxylin and eosin, and mounting with resin.

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    Tissues are composed of cells and extracellular matrix. Slides used in histology labs contain formalin-fixed, paraffin-embedded tissues stained with hematoxylin and eosin. The preparation of tissues for examination under a microscope involves fixation with formalin, dehydration with ethanol, clearing with xylene, infiltration and embedding with paraffin, sectioning, staining with hematoxylin and eosin, and mounting with resin.

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    5 views1 page

    Cells & Extracellular Matrix (H&E) - Stained Tissues

    Uploaded by

    Ishita guleria
    Tissues are composed of cells and extracellular matrix. Slides used in histology labs contain formalin-fixed, paraffin-embedded tissues stained with hematoxylin and eosin. The preparation of tissues for examination under a microscope involves fixation with formalin, dehydration with ethanol, clearing with xylene, infiltration and embedding with paraffin, sectioning, staining with hematoxylin and eosin, and mounting with resin.

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    © All Rights Reserved
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    of 1

    Tissues are made of two interacting components:

    Cells & extracellular matrix


    Slides used in the histology lab are of formalin-fixed, paraffin-embedded, hematoxylin & eosin
    (H&E)-stained tissues.
    Steps in preparation of tissues for examination by a (bright-field) compound microscope,
    &reagents used in each step, are:
    •Fixation (formalin)
    •Dehydration (ethanol)
    •Clearing (xylene)
    •Infiltration (paraffin)
    •Embedding (paraffin)
    •Sectioning (rotary microtome)
    •Staining (H&E)
    •Mounting (resin)

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