Sardar Patel University, Mandi

2nd Term Question Paper MSc Botany (1st semester)

Tools and Techniques in Biological Sciences (MBOT-104)

Total marks: 15 Time: 1.5 hr

Note: Section A is compulsory and attempt any two questions from section B
Section –A (2.5 x 2 =5)
Question 1: Explain the working principle of phase contrast microscopy?
Question 2: Differentiate between simple and compound microscopy?
Section –B (5 x 2 =10)
Question 3: Explain in detail croprotectants, freezing, verification involved in the process of
cryopreservation with the detailed application of cryopreservation?

Question 4: Explain in detail electron microscopy (either SEM or TEM), its working principle,
parts and applications with labelled diagrams?

Question 5: Explain in detail microtome knife, their types based on disposability, material used
for making knife, angle of knife and method of sharping?