Professional Documents
Culture Documents
By
Assist. Prof. Fatma Abbas Salem
Learning Objectives
19/12/2022
Contaminated – means dirty, soiled, unclean
19/12/2022
Colonization is used to describe
microorganisms present without host
interference or interaction. Organisms reported
in microbiology test result often reflect
colonization rather than infection
19/12/2022
The chain of infection: the necessary elements for
infection to occur are the following:
1. A Causative organism
2. A reservoir of available organisms
3. A mode of entry to host
4. Route of transmission
5. Mode of exit
6. A susceptible host
19/12/2022
A Causative organism, the types of
microorganisms that cause infections are
bacteria, rickettsia, viruses, protozoa, and
fungi
19/12/2022
A reservoir of available organisms, reservoir
is the term used for any person, plant, animal,
substance, or location that provides
nourishment for microorganisms and enables
further dispersal of the organism.
19/12/2022
A mode of entry to host
a portal of entry is needed for the organism to
gain access to the host
19/12/2022
Route of transmission, a route of transmission is
necessary to connect to the infectious source with its
new host. organisms may be transmitted through
sexual contact, skin to skin contact, percutaneuos
injection, or droplet infection
19/12/2022
Mode of exit, the organism must have a mode of exit
from a reservoir. Infected host must shed organisms
to another or to the environment before transmission
occur. organisms exit through the respiratory tract, the
gastrointestinal tract, the genitourinary tract or the
blood
19/12/2022
A susceptible host, for infection to occur, the
host must be susceptible. Previous infection or
vaccine administration may render the host
immune to further infection with an agent. a
person who is immunosuppressed has much
greater susceptibility to infection than a
healthy person
19/12/2022
I. At Cellular LeveL
1. death and lysis of cell
2. dying cell
3. severe impairment of cell function
4. moderate impairment of cell function
5. mild impairment of cell function
19/12/2022
At Host Response
1- death of host
2- septicemia or fulminates disease
3- severe disease require hospitalization
4- moderate illness requiring medical care
5- mild, ambulatory illness
19/12/2022
1- attachment, entry and
multiplication without cell injury
2- attachment with surface
multiplication
3- exposure without attachment to
cell
19/12/2022
1- infection without disease
2- colonization without tissue injury
3- exposure without multiplication of bacteria
19/12/2022
Preventing infection in the hospital
Isolation precautions , are guidelines created to
prevent transmission of microorganisms in
hospitals .the CDC recommends two tiers of
isolation precautions:
19/12/2022
standard precautions, is designed for the care of all
patients in the hospital and is the primary strategy for
preventing HAIS. The elements of standard precaution
include:-
19/12/2022
Hand hygiene
Glove use
needle stick prevention.
Mechanical devices
Avoidance of splash and spray
vaccination health care workers
19/12/2022
Use practical, common sense
19/12/2022
Wear gloves if you may come in contact with
blood, body fluids, secretions and excretions,
broken or open skin, human tissue of mucous
membranes
19/12/2022
Good hand washing is the most effective
method to prevent the spread of infection
19/12/2022
Cover your nose and mouth with a tissue
every time you cough or sneeze
19/12/2022
Remove any jewelry or watch
Wet hands with warm, running water
Add soap
Rub hands vigorously for 20 seconds,
washing all surfaces (about the time it takes
to sing “Happy Birthday” twice)
Rinse, keeping fingers pointing down
Dry with paper or clean cloth towel
Turn off faucet with towel and open door with
towel
19/12/2022
Make sure all visible dirt is removed from
your hands
Apply a dime sized amount of waterless hand
sanitizer to the palm of one hand or use a
waterless hand sanitizer wipe
Rub hands together covering all surfaces of
hands and fingers
Rub until waterless hand sanitizer is absorbed
Remember, waterless sanitizers are not
effective if dirt is visible on your hands
19/12/2022
View these videos on hand hygiene:
http://www.cdc.gov/CDCTV/HandsTogether/
19/12/2022
When you may come in contact with blood or
any body fluids, open wounds, or mucous
membranes
19/12/2022
When you have open sores or cuts on your
hands
19/12/2022
When touching surfaces that may be
contaminated
19/12/2022
Wear gloves when handling soiled linens
19/12/2022
Use appropriate receptacles for disposal
19/12/2022
1. Remove any sharp jewelry
19/12/2022
1. Pinch the palm of one glove and pull away
from the palm.
19/12/2022
4. DO NOT take the glove completely off.
19/12/2022
Click for video instruction for removing
contaminated gloves:
http://www.hse.gov.uk/mvr/myths/video/vid
eo9.htm
19/12/2022
Put on clean gloves
19/12/2022
Transmission –based precaution, is designed for
care of patients with known or suspected infectious
diseases spread by airborne, droplet or contact routes:
19/12/2022
are required for patients with presumed or proven
pulmonary TB, chickenpox, or other air borne
pathogen. When hospitalized patient should be in
room with negative pressure, the door should
remain closed, and health care worker should
wear protective mask at all times while in the
patient room.
19/12/2022
are used for organisms that can be transmitted
by close, face to face contact, such as
influenza or meningococcal meningitis. the
nurse should wear a face mask , but because
the risk of transmission is limited to close
contact the door may remain open
19/12/2022
are used for organisms that are spread by skin
to skin contact, such as antibiotics – resistant
organisms. Contact precautions are designed to
emphasize cautious technique and the use of
barriers for organisms that have serious
epidemiological consequences or those easily
transmitted by contact between health care
worker and patient
19/12/2022
Clostridium difficile , this spore-forming
bacterium has significant HAI. Infection is
usually preceded by antibiotics that disrupt
normal intestinal flora and allow the
antibiotics –resistant C. difficile spores to
proliferate within the intestine
19/12/2022
19/12/2022