NAME: SHARON CHEBET

REGISTRATION NUMBER: HB100/G/5550/18

COURSE: BACHELOR OF SCIENCE IN FORENSIC SCIENCE

UNIT: BIOSAFETY AND BIOETHICS

UNIT CODE: HFS 2415

 1. Microorganisms are considered to be dead if?

The cell membrane is important structure to bacterial cells as they serve as a barrier

to separate the inside of the cell from the outside. They also regulates what enter and

exits the cell. Since they are so important for bacterial survival, cells that have

damaged membranes are considered dead. This is by treating bacteria with

appropriate dyes (SYTO 9 and propidium iodide) and observing it using either a

microscope or a fluorescence spectrometer, where dead microorganisms appear red.

2. What are the safety equipment in a laboratory?

i) Eyewash stations- to provide quick access to decontamination to minimize

accidental exposure to chemicals.

ii) Lab coats- this provides a full body protection from hazardous material.

iii) Disposable gloves- to provide protection from chemicals and other irritants.

iv) Safety goggles- to protect eyes from being exposed to dangerous material.

v) Safety storage cabinets- it store flammable liquids, corrosives and other hazardous

materials.

3. What are the harmful effects of biohazards?

i) Fungal diseases e.g thrush

ii) Viral diseases e,g mumps, hepatitis

iii) Bacterial diseases e.g tuberculosis, tetanus, food poisoning

iv) Heart diseases- endocarditis

v) Brain diseases- encephalitis

4. What are the biosafety concerns of recombinant laboratory?

i) Risks related to containment

ii) Severity of the infection

iii) Nature of the work to be conducted

iv) Origin of the microorganisms

v) Route of exposure

5. What is the purpose of containment in a biomedical laboratory?

i) Use of good microbiological techniques and practices to protect the laboratory

workers and immediate laboratory environment from exposure to biological agents.

ii) Physical separation of laboratories from public access and designed ventilation

systems helps prevent airborne biological agents from migrating outside the

laboratory.

iii) Use of safety equipment, i.e biological safety cabinets, to provide containment of

infectious aerosols generated by many microbiological procedures.

iv) Use of personal protective equipment to supplement the containment provided by

laboratory practices and safety equipment.

v) Use of recommended biosafety level to give conditions under which the agent

should be handled safely.

6. What are the elements of Occupational Health Support Service for personnel

i) Management commitment and employee involvement

ii) Hazard prevention and control

iii) Worksite safety analysis

iv) Safety and health training

v) Hazard identification and assessment