F -X C h a n ge F -X C h a n ge

PD PD

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Laboratorium TUK SMK-SMAK Bogor - HPLC Hitachi Chromaster
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PUSAT PENDIDIKAN DAN PELATIHAN INDUSTRI

SEKOLAH MENENGAH KEJURUAN - SMAK BOGOR
LABORATORIUM TEMPAT UJI KOMPETENSI (TUK) SMAKBO

LAPORAN HASIL ANALISIS HPLC CHROMASTER

Nama : STD Thiamin 25 ppm Siti Hafizah Basmah - 13.4

Data File : D:\Thiamin\Result\b1 b4\Hasil Validasi B1B4 13.5.rslt\Cal_Vitamin B1004.dat
Method : D:\Thiamin\Method\untitled.met
Acquired : 10/7/2021 5:34:20 PM (GMT +07:00)
Printed : 10/11/2021 12:56:36 PM (GMT +07:00)

DAD-CH1 254 nm
Cal_Vitamin B1004
Retention Time

200 200
mAU

mAU
100 100

2.853

3.153
2.300

3.867
0.267

0.433

0.640

0.840

1.033

1.347

1.607

1.813

3.313

3.553
2.613

0 0

0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0

Minutes

DAD-CH1 254
nm Results
Retention Time Area Area % Height Height %
0.267 11612 0.13 646 0.05
0.433 32044 0.36 2436 0.20
0.640 57012 0.64 4578 0.37
0.840 50524 0.57 6724 0.55
1.033 113067 1.27 8903 0.72
1.347 331875 3.72 12592 1.02
1.607 97176 1.09 15070 1.23
1.813 181109 2.03 17398 1.42
2.300 691220 7.74 29450 2.40
2.613 5873197 65.79 977710 79.58
2.853 778940 8.73 87291 7.11
3.153 352499 3.95 35079 2.86
3.313 151563 1.70 15833 1.29
3.553 90183 1.01 10046 0.82
3.867 115433 1.29 4758 0.39

Totals
8927454 100.00 1228514 100.00
 F -X C h a n ge F -X C h a n ge
PD PD

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W

W
O

O
N

N
y

y
bu

bu
to

to
Laboratorium TUK SMK-SMAK Bogor - HPLC Hitachi Chromaster
ww

ww
om

om
k

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lic

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C

C
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w

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tr re tr re
.

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k e r- s o ft w a k e r- s o ft w a