Peptone water Nutrient Broth Nutrient agar Blood agar MacConkey agar Chocolate agar

BASAL LIQUID BASAL BASAL ENRICHED+ ENRICHED+ ENRICHED

DIFFERENTIAL+ DIFFERENTIAL+
INDICATOR INDICATOR
AUTOCLAE AUTOCLAVE AUTOCLAVE AUTOCLAVE AUTOCLAVE , AUTOCLAVE
TYNDALLIZATION

PEPTONE+WATER PEPTONE+WATER+ Nutrient Nutrient Na- Nutrient agar+

+NACL NACL+ LEAN MEAT Broth+ AGAR agar+10% TAUROCHOLATE(SELEC DEFIBRINATED
AGAR DEFIBRINATED TIVE, SUPPRESS THE AND
AND GROWTH OF GRAM + ANTICOAGULATE
ANTICOAGULATED BACT.)+ D BLOOD
BLOOD LACTOSE+NEUTRAL
RED (
DIFFERENTIATION
BETWEEN LACTOSE
FERMANTING OR NOT)
BACTERIAL BACTERIAL OTHER MEDIA TO TEST GROWTH OF GRAM - Haemophilus,
MOTILITY,INDOLE MOTILITY, OTHER PREPARATION HEMOLYTIC BACTERIA , Neisseria
TEST, OTHER MEDIA + TO TEST PORPERTIES AND DIFFERENTIATION
MEDIA PREPARATION PIGMENT GROWTH OF BETWEEN LF AND NLF
PREPARATION PRODUCTION SOME BACTERIA
7.4-7.5 7.4-7.5 7.5 7.5
NUTRIENT AGAR NUTRIENT AGAR
STERILIZED BY STERILIZED BY
AUTOCLAVING AUTOCLAVING
AND THEN COOL AND THEN COOL
DOWN TO 50 DOWN TO 75-80
DEGREE C DEGREE C
THEN ADD THEN ADD
ASEPTICALLY ASEPTICALLY
HUMAN / ANIMAL HUMAN /
STARILE BLOOD ANIMAL STARILE
BLOOD
Loeffler's Serum Dorsett's egg Robertson's cooked meat
Slope medium Lowenstein - Jensen's media media

ENRICHED ENRICHED ENRICHED+ SELECTIVE+DIFFERENTIAL ANAEROBIC+DIFFERENTIAL

INSPISSATION INSPISSATION INSPISSATION AUTOCLAVE

NUTRIENT BROTH NUTRIENT EGG( MAKES THE MEDIA ENRICHED)+ NUTRIENT BROTH +
+ BROTH + MALACHITE GREEN( SELECTIVE BY OX/SHEEP/HEART MUSCLE
OX/SHEEP/HORSE EGGS(HEN'S) SUPPRESS THE GROWTH OF ALL BACT. + 0.5% GLUCOSE
SERUM + EXCEPT MICOBACTERIA) + GLYCEROL(
GLUCOSE GROWTH, DIFFERENTIATION
BETWEEN HUMAN AND BOVINE TYPE
OF MICROBACTERIUM) + MINERAL
SALT SOLUTION

GROWTH OF MAINTAINS GROWTH AND DRUG SENSITIVITY OF GROWTH OF ANAEROBIC

DIPTHERIA AND LABORETORY M. TB BACTERIA
PROTEOLYTIC STAINS OF M.
ACTIVITY OF TB
CLOSTRIDIUM
PARASITOLOGY DRAWING
BACTERIA GRAM MOTILITY COAGULASE CONFIRMATORY INDOLE SUGAR UREASE MEDIA NATURE
TESTS
Staphylococcus +ve coccus Non motile Coagulase +ve Tube coagulase +ve Catalase Oxidase -ve Gelatinase,Phosphatase, Nutrient Colony are circular,
aureus are arranged +ve Dnase +ve agar convex,opaque,golden yellow
in cluster pigment
Bacillus subtilis +ve spore Motile Coagulase not Capsular staining reaction Nutrient Large circular,rough
bearing done ,no medusa hair agar pigmented margin irregular
bacilli, short appearance on nutrient greyish colour
chain , agar and no inverted far
spores are tree appearance on gelatin
central or media
subterminal
Pseudomonus -ve non motile Catalase,oxidase,nitrate Indole Glucose +(acid) Urease -ve Nutrient Smooth shiney translucent
aeruginosa sporing test , citrate test H2S +ve ring -ve Lactose and agar Moist
bacilli mannitol -ve Sweet grape like
haphazardly V.P test, MR test -ve

Escherichia coli Gram -ve motile Catalase ,nitrate test , V.P Indole Glucose and urease -ve MacConkey Consflucent and discrete
Straight rods test, MR test +ve +ve lactose acid + gas agar Smooth shiny non mucoid
haphazardly convex pink lactose
fermenting
Oxidase, citrate test H2S -
ve
Klebsiella Gram -ve Non motile Catalase ,nitrate test , V.P Indole - Glucose and Urease +ve MacConkey Consflucent and discrete
pneumoniae Straight rods test, citrate test +ve ve mannitol ,lactose agar circular
haphazardly acid + gas mucoid dome shaped pink
Oxidase MR test H2S -ve lactose fermenting
Salmonella typhi Gram -ve Non motile Catalase ,nitrate test MR Indole - Glucose and Urease -ve MacConkey Colour less and non lactose
Straight rods test H2S +ve ve mannitol acid agar fermenting
haphazardly
Oxidase, citrate test, V.P Lactose -ve
test-ve
Proteus vulgaris Gram -ve motile Catalase, nitrate, H2S Indole Glucose acid + Urease +ve MacConkey Consflucent and few discrete
Straight rods KCN sensitivity +ve +ve gas agar Circular small NLF
haphazardly low convex
Oxidase, PPA, ODC -ve mannitol , Smooth moist pale
Lactose -ve
Proteus mirabilis Gram -ve motile Catalase, nitrate, H2S INDOLE Glucose acid + Urease +ve MacConkey Consflucent and few discrete
Straight rods KCN sensitivityODC +ve -ve gas agar Circular small NLF
haphazardly low convex
Oxidase, PPA -ve mannitol , Smooth moist pale
Lactose -ve