Service and Repair Procedures

Certification / Verification Procedure

Certification / Verification Procedure

This Certification/Verification procedure is for the use of the Beckman Coulter Representative or
appropriately trained person to verify the operation of the instrument following a new installation,
at the end of each service visit, or upon certification.

Required Material
r Test Data Sheet
r Two size standards (one size nominally 10 to 20% of the size of the aperture tube, and one that
is any size within the dynamic range of the aperture tube.)

Information to be Recorded in the Test Data Sheet (TDS)

Record the following selected information in the Test Data Sheet (TDS).

r SOM Settings
r Waste Tank Settings
r Sample Delivery Settings
r Fill, Flush, and Drain Settings
r Aperture Resistance Settings
r Noise level and Background.

Maintenance
r Inspect the system for leaks, broken or frayed wiring, or any physical damage to
electromechanical assemblies. Repairs should be made before continuing with this procedure.
r View the Error Log and look for error messages. If you encounter error messages that may
indicate a problem, then the suspected assembly should be tested and inspected. Replace as
necessary.
r Fill the system. Look for error messages that might indicate a problem.
r Inspect tubing. Replace any deteriorating tubing.
r Purge and Adjust the metering pump via the software.
r Enter the Service mode and click on Diagnostic Settings. See Service Mode in Chapter 5,
Troubleshooting Guide (page 5-2) for details.
r Perform the Fixed 15k dummy load test.
r Ensure that the aperture tube, external electrode, and stir rod are free of damage, are clean
and fastened securely.
r Verify that the waste and diluent bottles are clean and free of damage.
r Under Service, select View Voltages . . . and measure all voltages via software.

4-36 MultisizerTM 4 Service Manual (PN A60936AA)

 Service and Repair Procedures
Certification / Verification Procedure 4

System Test
r Place a beaker of clean diluent under the aperture tube.
r On the toolbar select Service and then Instrument Schematic. Verify that the following
subassemblies are operating properly:

Valve Operations
r Verify that the system fills correctly and completely when the Fill System command is
activated in the software.
r Repeatedly activate the Flush Aperture Tube command. Verify in the instrument schematic
that the fluid level in the waste tank is incrementing.

Waste Tank
r When the waste tank has been half filled, activate the Empty Waste Tank command and verify
that the emptying cycle completes successfully.
r Verify that the Waste Tank reads 0% in the schematic when emptied. Calibrate the Tank if
needed.

Vacuum Regulator
r Under Run, Troubleshooting, select Sample Delivery Settings and set the vacuum level at Waste
Tank to 2.0” Hg. Verify the progress of this adjustment on the left panel. You might also verify
the vacuum level at the instrument schematic.
r Set the vacuum level at Waste Tank back to 6” Hg (default) and verify.

Door Switches
Verify that an error message is generated when you try to run an analysis with the door open.

Fans
From the instrument schematic, verify that the fans can be turned On and Off. After checking,
ensure that the fans are left on.

Stirrer Motor
Verify that the motor can be turned On and Off by raising and lowering the platform and that the
motor speed adjustment is working.

Aperture Lamp
Verify that the lamp can be turned On and Off in the instrument schematic panel.

MultisizerTM 4 Service Manual (PN A60936AA) 4-37

 Service and Repair Procedures
Certification / Verification Procedure

Ground
Verify that the ground terminal of the input filter module is secure and the resistance between the
ground terminal and chassis ground does not exceed 0.1 ohm.

Instrument Performance
r Perform a background count. Record the results on the Test Data Sheet.
r Verify Aperture Calibration on the two different size standards. Ensure that both results pass.
Results pass when the KD change is less than 3%. Record the results on the Test Data Sheet.
r Restore run settings to the customer’s original configuration.

Customer Review and Update

Review Preventative Maintenance with the customer. Cover the following subjects.

r Ensure that the aperture tube, external electrode, and stir rod are free of damage, are clean,
and fastened securely.
r Purge and adjust the metering pump via the software.
r Removal, cleaning and care of the aperture tube.

Customer Acceptance
• Have the customer run a sample or control if available, verify that the results are within the
expected range and that the instrument is operational.

NOTE
The customer is ultimately responsible for performing the final instrument calibration.

Certification Reporting
The certification procedure is performed yearly and at the time the service contract is renewed. It is
also performed when a customer requests a certification.

r Under Service select Update Multisizer Certification.

r From the Update Multisizer Certification dialog box, click on Mark as Certified button. Ensure
that the number of days is updated.

4-38 MultisizerTM 4 Service Manual (PN A60936AA)

 Service and Repair Procedures
Calibration/Adjustment Procedures 4

Figure 4.39 Update Certification

r Ensure that the correct phone number and Web page are entered.
r Exit the SERVICE mode.
r Fill out and sign the Certification Documents if appropriate. Place the Certification Sticker on
the instrument if appropriate.
r Leave a copy of the Test Data Sheet with the customer.

Calibration/Adjustment Procedures

Stirrer Position Calibration

r Move the Platform to the down position.
r Under Service select Configure Stirrer Position.
r Manually place the stirrer assembly all the way to the left.
r Click on Defaults.
r Click on Calibrate In and let the software perform the calibration routine.
r Once completed, click on Apply and then click on OK.

MultisizerTM 4 Service Manual (PN A60936AA) 4-39