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  • Mass Production of Beauveria

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    Bilge Deniz
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    The document discusses the mass production of the entomopathogenic fungus Beauveria for biological control. It describes preparing a mother culture by growing the fungus on SMAY media in test tubes for 10-15 days. For mass multiplication, the fungus is grown in bottles of sterile broth inoculated with the mother culture, then blended and mixed with a formulation like talc or oil. When packaged, the fungus spores can help control caterpillar pests by infecting insects like Spodoptera litura.

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    4. MASS PRODUCTION OF BEAUVERIA

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    The document discusses the mass production of the entomopathogenic fungus Beauveria for biological control. It describes preparing a mother culture by growing the fungus on SMAY media in test tubes for 10-15 days. For mass multiplication, the fungus is grown in bottles of sterile broth inoculated with the mother culture, then blended and mixed with a formulation like talc or oil. When packaged, the fungus spores can help control caterpillar pests by infecting insects like Spodoptera litura.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    22 views2 pages

    Mass Production of Beauveria

    Uploaded by

    Bilge Deniz
    The document discusses the mass production of the entomopathogenic fungus Beauveria for biological control. It describes preparing a mother culture by growing the fungus on SMAY media in test tubes for 10-15 days. For mass multiplication, the fungus is grown in bottles of sterile broth inoculated with the mother culture, then blended and mixed with a formulation like talc or oil. When packaged, the fungus spores can help control caterpillar pests by infecting insects like Spodoptera litura.

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    MASS PRODUCTION OF BEAUVERIA

    Entomopathogenic fungus used for the biological control of Lepidopteran


    caterpillars, grubs of pseudostem weevil of banana etc. The fungus is otherwise
    called as white muscardine fungus. The fungus spores and mycelia are milky
    white and found sprouting on the body of lepidopterous insects like Helicoverpa
    armigera, Spodoptera litura and Anadevidia peponis.

    PREPARATION OF MOTHER CULTURE

    • Media – SMAY (Sabourd’s Maltose Agar and


    Yeast) media
    • Media composition- Peptone(10g),
    Maltose(40g), Yeast(2g), Agar(20g), Distilled
    water(1000ml)
    • Sterilization of media at 121 degree Celsius for
    20 min in autoclave
    • Medium cooling
    • Test tubes kept in slanting position in Laminar air flow apparatus
    • Inoculation with 22 to 30 days old fungal disc
    • Incubation at room temperature for 10 to 15 days
    MASS MULTIPLICATION

    • Broth is poured at the rate of 300 ml per bottle


    • Sterilized at 121 degree Celsius for 20 min in autoclave Cooling
    • Inoculated with mother culture
    • Kept for 10 to 15 days at room temperature
    • Fungal growth along with broth collected and homogenized in a blender
    • Mixed with particular formulation (talc or oil)
    • Packed, sealed and labelled.

    Beauveria infested Spodoptera litura

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