DNA AND ITS

EXTRACTION

WHAT IT IS...?

Deoxyribonucleic Acid, is a large We need cells because

molecule made of nucleotides, that
together form a double helix shape,
is a type of nucleic acid, nucleic
acids are a type of biomolecules, the
nucleic acids are made up of
building blocks called nucleotides
thats where the DNA is,
the nucleus is inside of
almost all of our cells and
inside each nucleus is
about 2 meters of DNA

DNA EXTRACTION

COLLECT BURST CELLS SEPARATE DNA ISOLATED

OPEN TO Scientists isolate DNA first needs to
CHEEK CELLS FROM PROTEINS CONCENTRATED
RELEASE DNA DNA for many be purified away
AND DEBRIS DNA
reason, some of from proteins and
these are... other cellular
contaminants
Drag the swab It must be cut off the The cells have
over to the inside Inverting the tube
end of the swab in order sayed in the warm
of the test BODY several times mixes
that it will be able that water bath long
subject`s mouth IDENTIFICATION the isopropyl alcohol
the tube can close enough for the DNA
this to collect a into the DNA
to be freed from the
sample solution
cells, and add some
concentrated salt to
With the GENETIC your solution to your
micropipettor add INFORMATION tube
Place the swab some lysis solution
to the tube DNA is NOT
into the
soluble
Eppendorf tube ANALYSIS OF The salt causes
FORENSIC proteins and other
EVIDENCE cellular debris to
After the liquid is
Lysis: " to clump together
removed and the
separate" DNA is allowed to
Inside of the cheek
cell is a nucleus and dry, it has to be
inside the nucleus is re-dissolve it in the
a DNA Detergent: it Inside the solution
causes that Proteinase centrifuge the
K: cuts
cells burst tubes spine
apart the
open and around at high
histones to It can be
release the speed
free te DNA placed in the
DNA
freezer for
many years or
Use the be use inother
micropipettor experiment
to remove the
top liquid and
place it to a
clean tube