Biology Lab Report

Quarter 2 Laboratory Activity #2

Subject & Teacher: Biology 2, Mary Sheryl Saldon-Raznee

Group Number: 2

Group Members (Last name, First Name):

Adelante, Leila Loraine

Salomes, Godiva Bernice

Realiza, Aziraj Kent

Garcinces, Rhussel

Gelsano, Ethan

Tañan, Theojay Clarence

Laboratory Activity Name: Plant Cell Microscopy part II

Materials:

Microscope

Toothpicks/Cotton buds

Wet tissue

Surgical gloves

Methylene blue

Dropper

Glass and cover slips

Beaker

Pond water with microorganisms

Animal cells (procured from a subject’s mouth)

Algae
Procedure:

1.) Prepare glass slide and cover slip

2.) Double check if the cover slip is doubled with bare hands
3.) Wear surgical gloves

FOR THE FIRST ACTIVITY (Animal Cells):

4.) Using a dropper, put tiny drop methylene blue on the glass slide
5.) Procure animal cells
a. Dry a part of the cheeks inside the mouth with a towel or by airdrying (leaving
your mouth open for a minute)
b. Rub a toothpick or cotton bud along the inner cheek’s lining
6.) Blot the part of the toothpick which was used to collect cells on the area with
methylene blue on the glass slide

FOR THE SECOND ACTIVITY (Algae and Microorganisms):

4.) Select a small amount of algae from the given sample and extract it with a toothpick

5.) Place the algae on a glass slip and, after making sure that it makes a thin layer, cover it
with a cover slip

6.) Using a dropper, place a drop of the pond water which comes with the algae to seal the
coverslip

7.) Cover the sample with the cover slip

8.) Secure the glass slide with the sample on the microscope with the stage clips

9.) Turn on the microscope

10.) Start looking for the specimen with low power

11.) Locate the specimen using the course adjustment knob

12.) Locate the specimen using the stage adjustment knobs (for side-to-side movement)

13.) Repeat steps 11.) and 12.) for mid power

14.) Once a possible specimen has been located, switch to high power and repeat step 12.)
if needed

14.) Refine the resolution of the view of the specimen using the fine adjustment knob

15.) (Optional) Increase or decrease the lighting using the iris diaphragm for better
viewing of the specimen
16.) After finishing observing the specimen, clean the glass slides with gloves still on

17.) Leave glass slides on a safe place to air dry

18.) Turn off microscope

19.) Return microscope

20.) Clean working station

Results (post pictures and total resolution):

Animal Cells
160 magnification

Animal Cells Animal Cells

160 magnification 170 magnification
Analysis: (5pts each)

1.

Question: What are the 3 parts of the animal cell visible from your slide/s?

Answer: The parts of the animal cell seen from the microscope are the Cytoplasm, Plasma
membrane and the nucleus.

2.

Question: Why do we use methylene blue?

Answer: We use methylene blue to put more emphasis on the cells since it adds this pigment
that stains them. Without such, it would be transparent, resulting in an extremely harder time
locating what we were looking for.

3.

Question: How does the dye(methylene blue) work in staining the animal cell?

Answer: The dye itself stains the animal cell through the usage of positively charged dye
being attracted and staining the negatively charged particles/molecules within the cell
including the RNA and DNA.

4. .cv

Question: What microscopic organisms you found in the second activity? (list them and label
the images shown in the result section)

Answer: The microscopic organism we have found is most likely an arthropod, as stated by
the teacher.

5.

Question: Which domain do you think those microorganisms belong? (list them and label
their domain)

Answer: The domain which the aforementioned microorganism belongs to is most likely
Eukarya. Additionally, the green algae utilized in the experiment is part of the domain
Eukarya.