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General Biology 1
Quarter 2 Module 2
Photosynthesis: ATP & ADP Cycle and Pigments
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Republic of the Philippines
DEPARTMENT OF EDUCATION
Region VII, Central Visayas
DIVISION OF CITY SCHOOLS
City of Naga, Cebu
ATP is one of the most important compounds inside a cell because it is the energy
transport molecule. ATP (Adenosine TriPhosphate) is considered a transporter of
energy because when one of the phosphate groups is broken off, turning it into
Adenosine DiPhosphate (the Tri means 3 phosphate groups, the Di means 2
phosphate groups). When a phosphate breaks off, through chemical reactions in a
cell, energy is released which the plant uses for cellular processes and to power
many energy-requiring cellular reactions.
https://www.bealsscience.com/post/2015/02/26/photosynthesis-atp-and-adp-cycle
Plants, on the other hand, are experts at capturing light energy and using it to make
sugars through a process called photosynthesis. This process begins with the
absorption of light by specialized organic molecules, called pigments, that are found
in the chloroplasts of plant cells. Here, we’ll consider light as a form of energy, and
we'll also see how pigments – such as the chlorophylls that make plants green –
Learning Objectives:
In this module, you are expected to:
1. Explain coupled reaction processes and describe the role of ATP in energy
coupling and transfer
2. Explain the importance of chlorophyll and other pigments
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What I Know
(based on https://www.proprofs.com/quiz-school/quizreport.php?title=cell-respiration-
cycle-test)
1.Adenosine Diphosphate (ADP) is a(n)_____ level energy molecule in the scheme
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6.Animals and plants uses ATP - ADP cycle to carry out cellular respiration.
A. TRUE B. FALSE C. slightly correct D. none of these
7.Please observe the molecule attached to this question. How many Nitrogen atoms
are seen?
A. 5 B. 1 C.3 D.7
8.What is the name of the green structure in the Adenosine Triphosphate molecule?
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A. 1 B. 5 C.3 D.13
What’s In
1. What composes the ATP? How does ATP fuel the cellular processes?
describe the role of ATP in energy coupling and transfer. On the other hand,
What is the relationship of ATP and ADP cycle to photosynthesis? What is the
importance of chlorophyll and other pigments.?
What’s New!
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Activity 1: ATP-ADP Cycle
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What Is it!
Structure of Adenosine triphosphate
https://www.bealsscience.com/post/2015/02/26/photosynthesis-atp-and-adp-cycle).
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https://www.studyandscore.com/studymaterial-detail/atp-cycle-structure-and-role-of-
atp-in-biological-reactions
https://slideplayer.com/slide/9488865/
A cell can be thought of as a small, bustling town. Carrier proteins move substances
into and out of the cell, motor proteins carry cargoes along microtubule tracks, and
metabolic enzymes busily break down and build up macromolecules.
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Even if they would not be energetically favorable (energy-releasing, or exergonic) in
isolation, these processes will continue merrily along if there is energy available to
power them (much as business will continue to be done in a town as long as there is
money flowing in). However, if the energy runs out, the reactions will grind to a halt,
and the cell will begin to die.
Structure of ATP. At the center of the molecule lies a sugar (ribose), with the base
adenine attached to one side and a string of three phosphates attached to the other.
The phosphate group closest to the ribose sugar is called the alpha phosphate
group; the one in the middle of the chain is the beta phosphate group; and the one at
the end is the gamma phosphate group.
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Image credit: OpenStax Biology.
The three phosphate groups, in order of closest to furthest from the ribose sugar, are
labeled alpha, beta, and gamma. ATP is made unstable by the three adjacent
negative charges in its phosphate tail, which "want" very badly to get further away
from each other. The bonds between the phosphate groups are
called phosphoanhydride bonds, and you may hear them referred to as “high-
energy” bonds.
Hydrolysis of ATP
Why are the phosphoanhydride bonds considered high-energy? All this really means
is that an appreciable amount of energy is released when one of these bonds is
broken in a hydrolysis (water-mediated breakdown) reaction. ATP is hydrolyzed to
ADP in the following reaction:
Like most chemical reactions, the hydrolysis of ATP to ADP is reversible. The
reverse reaction, which regenerates ATP from ADP .
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Image of the ATP cycle. ATP is like a charged battery, while ADP is like a dead
battery. ATP can be hydrolyzed to ADP and Pi by the addition of water, releasing
energy. ADP can be "recharged" to form ATP by the addition of energy, combining
with Pi in a process that releases a molecule of water.
You can think of ATP and ADP as being sort of like the charged and uncharged
forms of a rechargeable battery (as shown above). ATP, the charged battery, has
energy that can be used to power cellular reactions. Once the energy has been used
up, the uncharged battery (ADP) must be recharged before it can again be used as a
power source. The ATP regeneration reaction is just the reverse of the hydrolysis
reaction:
We’ve mentioned that a bunch of free energy is released during ATP hydrolysis, but
just how much are we talking? ∆G for the hydrolysis of one mole of ATP into ADP
and
Reaction coupling
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How is the energy released by ATP hydrolysis used to power other reactions in a
cell? In most cases, cells use a strategy called reaction coupling, in which an
energetically favorable reaction (like ATP hydrolysis) is directly linked with an
energetically unfavorable (endergonic) reaction. The linking often happens through
a shared intermediate, meaning that a product of one reaction is “picked up” and
used as a reactant in the second reaction.
When two reactions are coupled, they can be added together to give an overall
reaction, and the ΔG of this reaction will be the sum of the ΔG values of the
individual reactions. As long as the overall ΔG is negative, both reactions can take
place. Even a very endergonic reaction can occur if it is paired with a very exergonic
one (such as hydrolysis of ATP). For instance, we can add up a pair of generic
reactions coupled by a shared intermediate, B, as follows:
A⇋ B ΔG=X
+ B⇋C+D ΔG= Y
A ⇋C ΔG=X+Y
You might notice that the intermediate, B, doesn't appear in the overall coupled
reaction. This is because it appears as a both a product and a reactant, so two Bs
cancel each other out when the reactions are added.
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Case study: Let's make sucrose!
How is the energy released in ATP hydrolysis channeled into the production of a
sucrose molecule? As it turns out, there are actually two reactions that take place,
not just one big reaction, and the product of the first reaction acts as a reactant for
the second.
In the first reaction, a phosphate group is transferred from ATP to glucose, forming a
phosphorylated glucose intermediate (glucose-P). This is an energetically favorable
(energy-releasing) reaction because ATP is so unstable, i.e., really "wants" to lose its
phosphate group.
In the second reaction, the glucose-P intermediate reacts with fructose to form
sucrose. Because glucose-P is relatively unstable (thanks to its attached phosphate
group), this reaction also releases energy and is spontaneous.
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Illustration of reaction coupling using ATP.
In the uncoupled reaction, glucose and fructose combine to form sucrose. This
reaction is thermodynamically unfavorable (requires energy).
When this reaction is coupled to ATP hydrolysis, it can take place, occurring in two
energetically favorable steps. In the first step, a phosphate group is transferred from
ATP to glucose, making the intermediate molecule glucose-P. Glucose-P is reactive
(unstable) and can react with fructose to form sucrose, releasing an inorganic
phosphate in the process.
This example shows how reaction coupling involving ATP can work through
phosphorylation, breaking a reaction down into two energetically favored steps
connected by a phosphorylated (phosphate-bearing) intermediate. This strategy is
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used in many metabolic pathways in the cell, providing a way for the energy released
by converting ATP to ADP to drive other reactions forward.
The example above shows how ATP hydrolysis can be coupled to a biosynthetic
reaction. However, ATP hydrolysis can also be coupled to other classes of cellular
reactions, such as the shape changes of proteins that transport other molecules into
or out of the cell.
It’s energetically unfavorable to move sodium out of, or potassium into, a typical cell,
because this movement is against the concentration gradients of the ions. ATP
provides energy for the transport of sodium and potassium by way of a membrane-
embedded protein called the sodium-potassium pump (Na+/K+ pump).
1. Three sodium ions bind to the sodium-potassium pump, which is open to the interior
of the cell.
2. The pump hydrolyzes ATP, phosphorylating itself (attaching a phosphate group to
itself) and releasing ATP. This phosphorylation event causes a shape change in the
pump, in which it closes off on the inside of the cell and opens up to the exterior of
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the cell. The three sodium ions are released, and two potassium ions bind to the
interior of the pump.
3. The binding of the potassium ions triggers another shape change in the pump, which
loses its phosphate group and returns to its inward-facing shape. The potassium ions
are released into the interior of the cell, and the pump cycle can begin again.
Image modified from The sodium-potassium exchange pump, by Blausen staff (CC
BY 3.0).
In this process, ATP transfers one of its phosphate groups to the pump protein,
forming ADP and a phosphorylated “intermediate” form of the pump. The
phosphorylated pump is unstable in its original conformation (facing the inside of the
cell), so it becomes more stable by changing shape, opening towards the outside of
the cell and releasing sodium ions outside. When extracellular potassium ions bind
to the phosphorylated pump, they trigger the removal of the phosphate group,
making the protein unstable in its outward-facing form. The protein will then become
more stable by returning to its original shape, releasing the potassium ions inside the
cell.
Although this example involves chemical gradients and protein transporters, the
basic principle is similar to the sucrose example above. ATP hydrolysis is coupled to
a work-requiring (energetically unfavorable) process through formation of an
unstable, phosphorylated intermediate, allowing the process to take place in a series
of steps that are each energetically favorable.
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The set of wavelengths absorbed by a pigment is its absorption spectrum. In the
diagram below, you can see the absorption spectra of three key pigments in
photosynthesis: chlorophyll a, chlorophyll b, and β-carotene. The set of wavelengths
that a pigment doesn't absorb are reflected, and the reflected light is what we see as
color. For instance, plants appear green to us because they contain many
chlorophyll a and b molecules, which reflect green light.
Chlorophylls
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Chlorophyll molecules absorb blue and red wavelengths, as shown by the peaks in
the absorption spectra above.
1.The most important being chlorophyll "a". This is the molecule which makes
2.A second kind of chlorophyll is chlorophyll "b", which occurs only in "green
algae" and in the plants.
3.A third form of chlorophyll which is common is (not surprisingly) called chlorophyll
"c", and is found only in the photosynthetic members of the Chromista as well as
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the dinoflagellates. The differences between the chlorophylls of these major groups
was one of the first clues that they were not as closely related as previously thought.
Carotenoids are usually red, orange, or yellow pigments, and include the familiar
compound carotene, which gives carrots their color. These compounds are
composed of two small six-carbon rings connected by a "chain" of carbon atoms. As
a result, they do not dissolve in water, and must be attached to membranes within
the cell. Carotenoids cannot transfer sunlight energy directly to the photosynthetic
pathway, but must pass their absorbed energy to chlorophyll. For this reason, they
are called accessory pigments. One very visible accessory pigment
is fucoxanthin the brown pigment which colors kelps and other brown algae as well
as the diatoms.
Carotenoids are another key group of pigments that absorb violet and blue-green
light (see spectrum graph above). The brightly colored carotenoids found in fruit—
such as the red of tomato (lycopene), the yellow of corn seeds (zeaxanthin), or the
orange of an orange peel (β-carotene)—are often used as advertisements to attract
animals, which can help disperse the plant's seeds.
In photosynthesis, carotenoids help capture light, but they also have an important
role in getting rid of excess light energy. When a leaf is exposed to full sun, it
receives a huge amount of energy; if that energy is not handled properly, it can
damage the photosynthetic machinery. Carotenoids in chloroplasts help absorb the
excess energy and dissipate it as heat.
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Because of the central role of chlorophyll a in photosynthesis, all pigments used in
addition to chlorophyll a are known as accessory pigments—including other
chlorophylls, as well as other classes of pigments like the carotenoids. The use of
accessory pigments allows a broader range of wavelengths to be absorbed, and
thus, more energy to be captured from sunlight.What does it mean for a pigment to
absorb light?
Only a photon with just the right amount of energy to bump an electron between
orbitals can excite a pigment. In fact, this is why different pigments absorb different
wavelengths of light: the "energy gaps" between the orbitals are different in each
pigment, meaning that photons of different wavelengths are needed in each case to
provide an energy boost that matches the gap^44start superscript, 4, end
superscript.
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When a pigment molecule absorbs light, it is raised from a ground state to an excited
state. This means that an electron jumps to a higher-energy orbital ( an orbital that is
further from the nucleus).
An excited pigment is unstable, and it has various "options" available for becoming
more stable. For instance, it may transfer either its extra energy or its excited
electron to a neighboring molecule. We'll see how both of these processes work in
the next section: the light-dependent reactions.
Phycobilins are not only useful to the organisms which use them for soaking up light
energy; they have also found use as research tools. Both pycocyanin and
phycoerythrin fluoresce at a particular wavelength. That is, when they are exposed
to strong light, they absorb the light energy, and release it by emitting light of a very
narrow range of wavelengths. The light produced by this fluorescence is so
distinctive and reliable, that phycobilins may be used as chemical "tags". The
pigments are chemically bonded to antibodies, which are then put into a solution of
cells. When the solution is sprayed as a stream of fine droplets past a laser and
computer sensor, a machine can identify whether the cells in the droplets have been
"tagged" by the antibodies. This has found extensive use in cancer research, for
"tagging" tumor cells.
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What’s More!
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https://www.liveworksheets.com/ct1239586p
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1. Explain coupled reaction processes and describe the role of ATP in energy
coupling and transfer
2. Explain the importance of chlorophyll and other pigments
What I Can Do
Make a poem on what you can help as a young leaner and A NAGAHANON in
maintaining and uplifting the Clean and Green Environment Program of the LGU.
(relate it to the no use of plastic in our community)
GENERAL BIOLOGY 1
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SECOND Quarter Week 2
Post Assessment
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A. 1 B. 5 C.3 D.13
6-7.
6.Which energy molecule has the most chemical energy? (refer to the illustration
above)
A.ATP B. ADP C. GTP D. AMP
7.Please identify the low energy molecule? (refer to the illustration above)
B. ATP B. ADP C. Pi (inorganic sugar) D.AMP
8.What is the name of this energy molecule?
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10. How many oxygen atoms are found in triphosphate group attached to the
adenosine triphosphate molecule found attached to this question?
11. These are chemical compounds which reflect only certain wavelengths of visible
light. This makes them appear "colorful". Flowers, corals, and even animal skin
contain pigments which give them their colors.
13. This is the molecule which makes photosynthesis possible, by passing its
energized electrons on to molecules which will manufacture sugars.
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Republic of the Philippines
DEPARTMENT OF EDUCATION
Region VII, Central Visayas
DIVISION OF CITY SCHOOLS
CITY OF NAGA, CEBU
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Republic of the Philippines
DEPARTMENT OF EDUCATION
Region VII, Central Visayas
DIVISION OF CITY SCHOOLS
CITY OF NAGA, CEBU
Prepared by:
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JUDIMA A. SUMAYANG
(Writer)
CARMENCITA B. LOPEZ
Education Program Supervisor in Science
(Content and Language Evaluator / Reviewer)
MERLY J. OMAMBAC
Education Program Supervisor in LRMDS
(Book Design/Format/Layout Evaluator / Reviewer)
Quality Assurance Team
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Republic of the Philippines
DEPARTMENT OF EDUCATION
Region VII, Central Visayas
DIVISION OF CITY SCHOOLS
CITY OF NAGA, CEBU
GENERAL BIOLOGY 1
SECOND QUARTER WEEK 2
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Republic of the Philippines
DEPARTMENT OF EDUCATION
Region VII, Central Visayas
DIVISION OF CITY SCHOOLS
CITY OF NAGA, CEBU
GENERAL BIOLOGY 1
SECOND QUARTER WEEK 2
References:
Campbell, N. Reece, B., Taylor M., ‘et.al’. Tour of the cell. Biology Concepts and
Connection. 10th ed. Pearson Education Inc. Sansome St., San Francisco, A9411.
2013
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