10th Malaysia-Indonesia-Brunei

Medical Sciences Conference 2017

"Current Healthcare Challenges in

South East Asia"

26-28 July 2017

Universiti Kebangsaan Malaysia

Medical Centre
Med & Health Jul 2017; 12(1) (Suppl): 1-149 https://doi.org/10.17576/MH.2017.s1201

Biomedical Sciences Oral

THE EXPANSION AND CHONDROGENIC DIFFERENTIATION

POTENTIAL OF MESENCHYMAL STEM CELLS ON GELATIN
MICROSPHERE: DYNAMIC VS STATIC CULTURE CONDITIONS

SHAMSUL SULAIMAN1, MOHD FAUZI MH BUSRA1, NG

MIN HWEI1 , RIZAL ABDUL RANI2 , NOR HAMDAN
MOHAMAD YAHAYA2 , YASUHIKO TABATA3, RUSZYMAH
IDRUS4, SHIPLU ROY CHOWDHURY1

Tissue Engineering Centre, 2Department of Orthopedic & Traumatology,

1

4
Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia,
Clinical Block, Jalan Yaacob Latiff, 56000 Cheras, Kuala Lumpur, Malaysia.
3
Department of Biomaterials, Institute for Frontier Medical Sciences, Kyoto
University, 53 Kawara-cho Shogoin, Sakyo-ku Kyoto 606-8507, Japan

Introduction: Mesenchymal stem cells (MSCs) known to have criteria as self-

renewal ability in monolayer culture and can be differentiated into mesodermal
lineages, including osteogenic, adipogenic, and chondrogenic. However,
numerous studies have reported a loss of the mitotic ability and capacity of MSCs
to differentiate in monolayer culture. Thus, the aim of the study was to evaluate
the expansion and chondrogenic differentiation potential of MSCs cultured on
gelatin microsphere (GM) under static and dynamic conditions. Methodology:
MSCs was isolated from the bone marrow sample of consented patients. Cells were
seeded with GM, and cultured with chondrogenic induction medium (CIM) under
static and dynamic conditions. Cell attachment, proliferation, and chondrogenic
differentiation were evaluated via confocal microscopy, presto blue assay and RT-
PCR (expression of collagen type I and II and aggrecan), respectively. Moreover,
sulfated glycosaminoglycan  (sGAG) production was also measured to confirm
the chondrogenic differentiation. Results: MSCs attached and spreaded on the
microspheres. Cells formed large cell–microsphere clusters with strong cell–cell and
cell-matrix interactions. We demonstrated higher attachment and proliferation of
MSCs on GM under dynamic condition as compared to that under static condition.
Moreover, expression of collagen type II, aggrecan genes and production of
sGAG were higher when cultured under dynamic condition as compared to static
condition. Conclusion: MSCs on GM under dynamic culture condition facilitates
cell proliferation and chondrogenic differentiation.

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