PMID- 16922713

OWN - NLM
STAT- MEDLINE
DCOM- 20070404
LR - 20211203
IS - 0742-3071 (Print)
IS - 0742-3071 (Linking)
VI - 23
IP - 9
DP - 2006 Sep
TI - Relationship between common functional polymorphisms of the p22phox gene (-
930A >
G and +242C > T) and nephropathy as a result of Type 2 diabetes in a Chinese
population.
PG - 1037-41
AB - OBJECTIVE: Genetic determinants are important in diabetic nephropathy (DN).
Oxidative stress has also emerged as an important pathogenic factor in DN and

vascular NADH oxidase is a major source of reactive oxygen species (ROS).

Previous small studies reported a strong but contradictory association
between
functional genetic variation of p22(phox), an important subcomponent of NADH
oxidase, and DN. We investigated the association between two common
functional
single nucleotide polymorphisms (SNPs) (-930 A > G and +242 C > T) and DN in
a
much larger group of Chinese patients with Type 2 diabetes mellitus (T2DM).
RESEARCH DESIGN AND METHODS: Case-control study of Chinese subjects with
long-standing T2DM (> 10 years). Cases (n = 306) were subjects with a spot
urinary albumin : creatinine ratio (ACR) of > 113 mg/mmol or elevated serum
creatinine. Control subjects (n = 306) had ACR < 3.3 mg/mmol and normal serum

creatinine. Genotyping was carried out by standard PCR and restriction

fragment
length polymorphism analysis. RESULTS: Gender distribution, age, duration of
diabetes and HbA(1c) were similar in cases and control subjects. Distribution
of
genotypes in the control subjects for both SNPs was consistent with the
Hardy-Weinberg equilibrium. Distribution of genotypes did not differ
significantly between cases and control subjects for both polymorphisms-
+2424C >
T: cases CC 84.6%, CT 15.0%, TT 0.4% and control subjects CC 87.6%, CT 11.8%,
TT
0.6% (P = 0.45); -930 A > G: cases AA 40.5%, AG 41.8%, GG 17.7% and control
subjects AA 38.2%, AG 49.0%, GG 12.8% (P = 0.12). Distribution of alleles was

also similar-+2424 C > T: cases C 92.2%, T 7.8% and control subjects C 93.5%,
T
6.5% (P = 0.66); -930 A > G cases A 61.4%, G 38.6% and control subjects A
62.7%,
G 37.3% (P = 0.38). We estimated that our study has approximately 80% power
to
detect a relative risk of 1.65 (for +242 C > T) and 1.35 (for -930 A > G)
conferred by the minor allele, respectively. CONCLUSIONS: In contrast with
previous small studies, our data suggest that these SNPs do not confer
significantly increased susceptibility to DN secondary to T2DM in Chinese
subjects.
FAU - Lim, S C
AU - Lim SC
AD - Department of Medicine, Alexandra Hospital, Singapore.
Su_chi_lim@alexhosp.com.sg
FAU - Goh, S K
AU - Goh SK
FAU - Lai, Y R
AU - Lai YR
FAU - Tee, W W
AU - Tee WW
FAU - Koh, A
AU - Koh A
FAU - Xu, X H
AU - Xu XH
FAU - Wu, Y S
AU - Wu YS
FAU - Yap, E
AU - Yap E
FAU - Subramaniam, T
AU - Subramaniam T
FAU - Sum, C F
AU - Sum CF
LA - eng
PT - Journal Article
PT - Multicenter Study
PL - England
TA - Diabet Med
JT - Diabetic medicine : a journal of the British Diabetic Association
JID - 8500858
RN - EC 1.6.3.- (NADPH Oxidases)
RN - EC 1.6.3.1 (CYBA protein, human)
SB - IM
MH - Aged
MH - Asians/*genetics
MH - Case-Control Studies
MH - Diabetes Mellitus, Type 2/ethnology/*genetics
MH - Diabetic Nephropathies/ethnology/*genetics
MH - Female
MH - Gene Frequency
MH - Genetic Predisposition to Disease
MH - Genotype
MH - Humans
MH - Male
MH - Middle Aged
MH - NADPH Oxidases/*genetics
MH - *Polymorphism, Single Nucleotide
MH - Singapore/epidemiology
EDAT- 2006/08/23 09:00
MHDA- 2007/04/05 09:00
CRDT- 2006/08/23 09:00
PHST- 2006/08/23 09:00 [pubmed]
PHST- 2007/04/05 09:00 [medline]
PHST- 2006/08/23 09:00 [entrez]
AID - DME1916 [pii]
AID - 10.1111/j.1464-5491.2006.01916.x [doi]
PST - ppublish
SO - Diabet Med. 2006 Sep;23(9):1037-41. doi: 10.1111/j.1464-5491.2006.01916.x.