TEST FOR NUTRIENTS [comparison]

Particulars Starch Sugar Protein Fat VITAMIN C

Name of Test Iodine Test Benedicts Test Biuret Test Ethanol / Emulsion DCPIP TEST
Test
Name of Iodine Benedicts Biuret Solution Ethanol DCPIP SOLUTION
Reagent Reagent
Colour of Brown Blue Blue Colourless Blue
Reagent
Procedure Take 2ml of Take 2ml of sugar Take 2ml of protein Take 3ml of fat/oil Take 3ml of DCPIP
starch solution and add solution and add few solution and solution and pour drops
solution and 2ml of benedict’s drops of biuret solution dissolve in ethanol. of Vitamin C
add iodine solution. (add potassium Add few drops of solution
drops Heat it gently hydroxide and drops of water.
copper sulphate)
Observation Deep blue/ Colour changes Purple/ mauve / lilac Milky emulsion / The solution becomes
(Positive result) bluish black from blue to colour is observed Turbid colour is colourless.
colour is green – yellow – formed.
observed orange- Red (red
ppt on cooling)
Negative result Brown Blue Blue Colourless Blue

Control Take 2ml of Take 2ml of water Take 2ml of water and Take 3ml of water Take 3ml of DCPIP
experiment water and and add 2ml of add few drops of biuret and dissolve in solution and pour drops
add iodine benedict’s solution (add potassium ethanol. of water
drops solution. Heat it hydroxide and drops of Add few drops of solution
gently copper sulphate) water.
Control experiment is the similar experimental setup without a factor under observation. It is used to check the validity of the
experiment.