James V of Scotland (1512) m.

Hedwig of Poland (1513)

Children

1. Stanislaus I of Scotland (11 January 1534) m. Eleanor of Austria

Children
● Sibylle (3 February 1550)
● Stanislaus II ( 12 July 1552)
● Ludmila (6 September 1564)

2. Isabelle (5 January 1534) m. Stephen Bathory

Children
● Dorothy (2 February 1560)
● Stanislaus I ( 4 July 1562)
● Ludmila (6 September 1564)

3.John Sigismund (5 January 1535) m. Joanna of Austria

Children
● Constantine (31 May 1564)
● Agnes ( 20 April 1566)
● Thomas ( 3 August 1569)

4. Beatrice ( 5 October 1536) m. Thomas Howard

Konrad of Poland (1515) m. Mary I of England (1516)

Children

1. Helena (16 July 1538) m. João Manuel of Portugal (1559)

Children
● Casimir I of Portugal and Dorothy (2 February 1560)
● Sigismund ( 4 July 1562)
● Ludmila (6 September 1564)
● Stephen (5 October 1566)

2. Barbara (2 May 1540) m. Jan Zamoyski (1563)

Children
● Constantine (31 May 1564)
● Agnes ( 20 April 1566)
● Thomas ( 3 August 1569)

3. Andrew (13 May 1543) m. Isabella de’ Medici (1564)

Children
● Matilda ( 18 September 1565)
● Beatrice ( 24 April 1568)
● Claudia ( 7 August 1570)
● Sophia (16 October 1572)

4. Louise (1 June 1545) m. Henry Stuart, Lord Darnley (1566)

Children
● Paul ( 4 December 1567)
● Matthias ( 12 December 1569)
● Joanna (3 May 1572)
● Joseph (25 March 1575)
5. John Ladislaus (1 June 1545) m. Lettice Knollys (
Children
● Paul ( 4 December 1567)
● Matthias ( 12 December 1569)
● Joanna (3 May 1572)
● Joseph (25 March 1575)
Sigismund II Augustus (1520) m. Dorothea of Denmark (1520)

Children

1. Alphons I, (20 January 1536) m. Margaret of Austria (1555)

Children
● Michael (4 December 1556)
● Christopher ( 5 June 1558)
● Amelia ( 16 July 1560)
● Maria Christina ( 24 May 1562)

2. Andrew (13 May 1538) m. Maria de’ Medici (1557)

Children
● Boleslaus (21 October 1558)
● Maria Helena ( 6 May 1560)
● Philip ( 12 June 1562)
● Anastasia ( 24 May 1564)

3. Wenceslaus (15 August 1544) m. Mary Stuart (1564)

Children
● Mary Margaret (7 October 1565)
● David ( 6 May 1567)
● Irene ( 12 June 1569)
● Albert ( 24 May 1570)

4. Agatha (4 June 1546) m. Cyprian of Tunis (1566)

Children
● John Dominic (16 November 1567)
● Adrian ( 20 April 1570)
● Elizabeth ( 3 August 1573)
● Monica ( 19 June 1576)
5. Alexander (5 July 1550) m. Maria Ivanovna (1571)
Children
● Joanna ( 15 September 1572)
● Nicholas ( 20 April 1574)
● Joseph ( 3 August 1576)
● Stephen (13 October November 1578)

6. Judith (8 September 1552) m. Henry Valois (1573)

Children
● Francis ( 4 October 1574)
● Casimir ( 8 December 1577)
● Adelaide (28 August 1580)
● Charles (7 March 1582)
William of Jülich-Cleves-Berge (1516) m. Sophia of Poland (1522)

Children

1. Francis of Jülich-Cleves-Berge ( 12 July 1543) m. Elizabeth of Valois

Children
● Michael (4 December 1556)
● Christopher ( 5 June 1558)
● Amelia ( 16 July 1560)
● Maria Christina ( 24 May 1562)

2. John (4 September 1544) m. Anastasia of Tripolitania

Children
● Boleslaus (21 October 1558)
● Maria Helena ( 6 May 1560)
● Philip ( 12 June 1562)
● Anastasia ( 24 May 1564)

3. Martha ( 13 October 1546) m. Eitel Friedrich IV

Children
● Mary Margaret (7 October 1565)
● David ( 6 May 1567)
● Irene ( 12 June 1569)
● Albert ( 24 May 1570)

4. Maria Helena (22 August 1547)

Children
● John Dominic (16 November 1567)
● Adrian ( 20 April 1570)
● Elizabeth ( 3 August 1573)
● Monica ( 19 June 1576)
5. Herman ( 4 November 1548)
Children
● Joanna ( 15 September 1572)
● Nicholas ( 20 April 1574)
● Joseph ( 3 August 1576)
● Stephen (13 October November 1578)

6. Ferdinand Casimir (10 June 1550)

Children
● Francis ( 4 October 1574)
● Casimir ( 8 December 1577)
● Adelaide (28 August 1580)
● Charles (7 March 1582)

7. Frederick Augustus (1 September 1551)

Children
● Francis ( 4 October 1574)
● Casimir ( 8 December 1577)
● Adelaide (28 August 1580)
● Charles (7 March 1582)