RBC COUNT HEMATOCRIT DETERMINATION

WINTROBE
RBC count/uL = # of cell counted x DF x Depth x Area
𝐋𝟏 (𝐡𝐞𝐢𝐠𝐡𝐭 𝐨𝐟 𝐫𝐞𝐝 𝐜𝐞𝐥𝐥 𝐜𝐨𝐥𝐮𝐦𝐧 − 𝐦𝐦)
𝐇𝐜𝐭 (𝐄𝐅𝐕) = 𝒙 𝟏𝟎𝟎
RBC no. conc. → SI 𝐋𝟐 (𝐡𝐞𝐢𝐠𝐡𝐭 𝐨𝐟 𝐰𝐡𝐨𝐥𝐞 𝐛𝐥𝐨𝐨𝐝 − 𝐦𝐦)
RBC count x .000001 = _______ x 1012/L or
𝐯𝐨𝐥𝐮𝐦𝐞 𝐨𝐟 𝐏𝐑𝐁𝐂𝐬
𝐇𝐜𝐭 (%) = 𝒙 𝟏𝟎𝟎
NORMAL VALUES 𝐯𝐨𝐥𝐮𝐦𝐞 𝐨𝐟 𝐰𝐡𝐨𝐥𝐞 𝐛𝐥𝐨𝐨𝐝 𝐮𝐬𝐞𝐝
MEN: 5.0-6.2 x 1012/L
WOMEN: 4.0-5.0 x 1012/L
RBC INDICES
PREGNANT: 7.0 x 1012/L
MEAN CELL VOLUME

𝐇𝐜𝐭
𝐌𝐂𝐕 = 𝒙 𝟏𝟎 (𝒇𝑳)
𝐑𝐁𝐂 𝐜𝐭 𝐢𝐧 𝐒𝐈
WBC COUNT
NORMAL VALUES
# 𝒐𝒇 𝒄𝒆𝒍𝒍 𝒄𝒐𝒖𝒏𝒕𝒆𝒅 𝒙 𝑫𝒆𝒑𝒕𝒉 𝒙 𝑫𝑭
WBC count/uL = 80 to 100fL
𝑨𝒓𝒆𝒂
MICROCYTIC: < 80fL
WBC no. conc. → SI MACROCYTIC: >100fL
RBC count x .001 = _______ x 109/L MEGALOCYTIC: >120fL

CORRECTED WBC COUNT MEAN CELL HEMOGLOBIN

𝑨𝒄𝒕𝒖𝒂𝒍 𝑾𝑩𝑪 𝒄𝒐𝒖𝒏𝒕 𝒙 𝟏𝟎𝟎 𝐇𝐠𝐛 (𝐠/𝐝𝐋)

Corrected WBC count/uL = 𝐌𝐂𝐇 = 𝒙 𝟏𝟎𝟎 (𝒑𝒈)
𝒏𝑹𝑩𝑪+𝟏𝟎𝟎 𝐑𝐁𝐂 𝐜𝐭 𝐢𝐧 𝐒𝐈

or Corrected HGB = Actual HGB – (HCT x HGB)

Corrected WBC count/uL = Actual WBC count – nRBC/uL NORMAL VALUES

26 to 32 pg
𝒏𝑹𝑩𝑪
nRBC = 𝒏𝑹𝑩𝑪+𝟏𝟎𝟎 𝒙 𝑨𝒄𝒕𝒖𝒂𝒍 𝑾𝑩𝑪 𝒄𝒐𝒖𝒏𝒕 HYPOCHROMIC: < 26pg
HYPERCHROMIC: >32pg
NORMAL VALUES
ADULT: 4.5-11.0 x 109/L MEAN CELL HEMOGLOBIN CONCENTRATION
INFANT: 10-25 x 109/L
𝐇𝐠𝐛
𝐌𝐂𝐇𝐂 = 𝒙 𝟏𝟎𝟎 (𝒈/𝒅𝑳)
𝐇𝐜𝐭

WBC DIFFERENTIAL COUNT NORMAL VALUES

RELATIVE COUNT 32 to 36 g/dL
HYPOCHROMIA: 36 g/dL
# 𝒐𝒇 𝑾𝑩𝑪 𝒕𝒚𝒑𝒆 HYPERCHROMIC: >36 g/dL
Relative Count = 𝒙 𝟏𝟎𝟎
𝟏𝟎𝟎

Relative Count. → SI
WBC count x .01 = _______ x 109/L

ABSOLUTE COUNT
𝑹𝒆𝒍𝒂𝒕𝒊𝒗𝒆 %
Absolute Count = 𝒙 𝑾𝑩𝑪 𝒄𝒐𝒖𝒏𝒕
𝟏𝟎𝟎

Absolute Count → SI
WBC count x .001 = _______ x 109/L

NORMAL VALUES
 PLATELET COUNT
INDIRECT METHOD
(Fonio, Dameshek, Olef)

𝐏𝐥𝐚𝐭𝐞𝐥𝐞𝐭 𝐱 𝐑𝐁𝐂 𝐜𝐭.

𝐏𝐥𝐚𝐭𝐞𝐥𝐞𝐭𝐬 /𝐮𝐋 =
𝟏𝟎𝟎𝟎
𝐡𝐢𝐠𝐡𝐞𝐬𝐭−𝐥𝐨𝐰𝐞𝐬𝐭
PLT ct/uL = (𝑎𝑣𝑒𝑟𝑎𝑔𝑒 # 𝑜𝑓 𝑝𝑙𝑎𝑡𝑒𝑙𝑒𝑡) 𝑥 20, 000
𝟐

Normal Value
Microliter Liter
250,000 – 500,000 /uL 250 – 500 x 109/L

DIRECT METHOD
(Guy and Leake, Rees and Ecker, Brecker-Cronkite)

total number of cells counted x dilution

𝐍𝐨. 𝐨𝐟 𝐜𝐞𝐥𝐥𝐬 /𝑚𝑚3 =
area (1 mm2) x depth (0.1 mm)

Normal Value
Microliter Liter
(Conventional) (SI)
150,000 – 450,000 /uL 150 – 450 x 109/L
↓
150,000,000,000 /L
150 – 450 x 103/L
↓
150,000-45,000 /uL

Anemic/Erythrocytosis

𝑎𝑣𝑒𝑟𝑎𝑔𝑒 # 𝑜𝑓 𝑝𝑙𝑎𝑡𝑒𝑙𝑒𝑡𝑠 𝐱 𝐑𝐁𝐂 𝐜𝐭.

PLT ct/uL =
𝟐𝟎𝟎 𝐑𝐁𝐂𝐬/𝐟𝐢𝐞𝐥𝐝

RETICULOCYTE COUNT
% RETICULOCYTE

# 𝒐𝒇 𝒓𝒆𝒕𝒊𝒄𝒖𝒍𝒐𝒄𝒚𝒕𝒆𝒔
% 𝑹𝒆𝒕𝒊𝒄𝒖𝒍𝒐𝒄𝒚𝒕𝒆 = 𝒙 𝟏𝟎𝟎
𝟏𝟎𝟎𝟎

Miller Disc:
# 𝒐𝒇 𝒓𝒆𝒕𝒊𝒄𝒖𝒍𝒐𝒄𝒚𝒕𝒆𝒔 𝒊𝒏 𝒍𝒂𝒓𝒈𝒆 𝒔𝒒𝒖𝒂𝒓𝒆 𝒙 𝟏𝟎𝟎
% 𝑹𝒆𝒕𝒊𝒄𝒖𝒍𝒐𝒄𝒚𝒕𝒆 =
# 𝒐𝒇 𝑹𝑩𝑪 𝒊𝒏 𝒔𝒎𝒂𝒍𝒍 𝒔𝒒𝒖𝒂𝒓𝒆 𝒙 𝟗

ABSOLUTE RETICULOCYTE COUNT (ARC)

% 𝒓𝒆𝒕𝒊𝒄𝒖𝒍𝒐𝒄𝒚𝒕𝒆𝒔 𝒙 𝑹𝑩𝑪 𝒄𝒐𝒖𝒏𝒕 (𝒙 𝟏𝟎𝟏𝟐 )

𝑨𝑹𝑪 =
𝟏𝟎𝟎

Reticulocyte Number Concentration: ARC x .001 = __ x 109/L

CORRECTED RETICULOCYTE COUNT

% 𝒓𝒆𝒕𝒊𝒄𝒖𝒍𝒐𝒄𝒚𝒕𝒆𝒔 𝒙 𝒑𝒂𝒕𝒊𝒆𝒏𝒕 𝑯𝑪𝑻

𝑪𝑹𝑪 =
𝟒𝟓

RETICULOCYTE PRODUCTION INDEX

𝑪𝑹𝑪
𝑹𝑷𝑰 =
𝒎𝒂𝒕𝒖𝒓𝒂𝒕𝒊𝒐𝒏 𝒕𝒊𝒎𝒆
!!! NO UNIT !!!
MATURATION TIME OF
HCT
RETICS IN BLOOD
45% ± 5% (40-50) 1.0 days
35% ± 5% (30-40) 1.5 days
25% ± 5% (20-30) 2.0 days
15% ± 5% (10-20) 2.5 days
5% ± 5% (10-20) Patient is dead