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2ND QUARTER
PERFORMANCE TASK# 3
CELLULAR METABOLISM

NAME: Ortega, Jana Marie D. SUBMITTED TO: KELLY JANSON

DATE SUBMITTED: DECEMEBER 19, 2022

You will be examining photosynthesis in an aquatic plant using dissolved oxygen (DO).

Go to the cell energy simulation at Biology Simulations.

I. Background
Plants perform both photosynthesis and cellular respiration.

1. How will photosynthesis affect oxygen levels?

It converts carbon dioxide and water intro glucose and oxygen as the resource of the plant.

2. How will cellular respiration affect oxygen levels?

Cellular respiration converts oxygen and glucose into water and carbon dioxide, and ATP is the
energy that is transformed during the process.

II. Respiration
Find the respiration for the plant.
1. Set the fish number to 0 and the plant number to 1.
2. Set the light intensity to 0% (With no light, there will be no photosynthesis and all changes to oxygen will
be due to respiration).
3. Leave the other variables at the default values (25 degrees, white light)
4. Run the simulation three times, recording the starting and ending oxygen values in the table.
5. Find the average difference to determine the respiration per hour.

Trial DO Start DO End DO Difference

1 7.0779 6.8929 0.185

2 7.0267 6.8327 0.194

3 7.0347 6.8485 0.1862

Average 7.04 6.86 0.19

Average DO Difference = Respiration / hour

Respiration / hour = 0.19mg/L/hour = There is 0.19 mg/L respiration per hour

III. Light Intensity

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Experimental Question: How does light intensity affect oxygen production?

1. Write your hypothesis.

Hypothesis: I think if the intensity of light is higher, then the production of oxygen will increase its amount
being its amount being produced under the process of photosynthesis, creating a favorable
environment for the plants?

2. Set the fish number to 0 and the plant number to 1. Leave the other variables at the default values (25
degrees, white light).
3. Set the light intensity to 100%.
4. Record the starting and ending oxygen values.
5. Repeat steps 3 and 4 for 80%, 60%, 40%, and 20%.
6. Calculate and record the DO difference, Oxygen (O2) production (in 60 minutes), and Oxygen production
per minute.

Light Intensity (%) DO start DO end DO Difference O2 Production* O2 Production/min

100 7.0815 7.49 -0.41 -0.45 -0.0075

80 7.084 7.29 -0.21 -0.26 -0.0042

60 7.0953 7.09 -0.0017 -0.05 -0.0008

40 7.039 6.84 0.19 0.15 -0.0024

20 7.0334 6.84 0.19 0.14 0.0024

*O2 Production = DO Difference + Respiration

7. Insert a graph displaying how light intensity affects O2 production per minute.

8. Write a sentence conclusion.

The expanding amount of light intensity corresponds to the increasing amount of oxygen production from
photosynthesis, which means the higher the light intensity, the more amount of oxygen are being produced.

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9. Write a paragraph explaining your conclusion in terms of the process of photosynthesis.

Plants contain green pigment that absorbs the solar of the sun or sunlight called chloropyll, these are tiny
structures in plant’s cell, located in a plant’s chloroplast . It’s classified as chlorins, which are reduced relatives or
porphyrins and its purpose is to absorb light that stores energy that converts it into carbon dioxide, absorbed
from the environment, and water glucose. Under this broader definition, I claim that the increased light
intensity also corresponds to the oxygen production which means it also increases and happens during
photosynthesis.

IV. Light Color

Experimental Question: How does light color affect oxygen production?

1. Write your hypothesis.

Hypothesis: If the light is higher based on the light spectrum, like blue and violet, then it would present us the
fastest rate of photosynthesis because they have the shortest wavelength, followed by green,
yellow, orange, and red.

2. Set the fish number to 0 and the plant number to 1. Leave the other variables at the default values
(100% light intensity, 25 degrees).
3. Set the light color to white (combination of all wavelengths/colors).
4. Record the starting and ending oxygen values.
5. Repeat steps 3 and 4 for violet, blue, green, yellow, orange, and red light.
6. Calculate and record the DO difference, Oxygen (O2) production (in 60 minutes), and Oxygen production
per minute.

Light Color DO start DO end DO Difference O2 Production* O2 Production/min

White 7.0304 7.4436 -0.4032 -0.6022 -0.10036

Violet 7.0472 7.1498 -0.1026 -0.3016 -5.0266

Blue 7.0727 7.1805 -0.1078 -0.3068 -5.1133

Green 7.0449 6.8531 -0.1918 -0.3908 -6.5133

Yellow 7.0796 6.8849 -0.1947 -0.3937 -6.5616

Red 7.0544 7.1141 -0.1393 -0.1393 -2.3216

*O2 Production = DO Difference + Respiration

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7. Insert a graph displaying how light color affects O2 production per minute.

8. Write a sentence conclusion.

The results show that each wavelength of light has a different impact on oxygen production by Clamydomonas
reinhardtii.

9. Write a paragraph explaining your conclusion in terms of the process of photosynthesis.

Clamydomonas reinhardtii plays an essential role in aquatic ecosystems. Exposure to white light producs the
highest water oxygen content, followed by green light, blue light, ang lastly redlight. This means the higher the
intensity of light, the more will be the production of oxygen in the form of bubbles by the plants under the
process of photosynthesis. Therefore, when you reduce the distance between the light and plant, there are
more oxygen bubbles produced by the plants.

V. Temperature
Experimental Question: How does temperature affect oxygen production?

1. Write your hypothesis.

Hypothesis: If there is an increase of temperature during the process, then the increase of oxygen also rises,
and if the temperature decreases, the oxygen production also decreases.

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2. Using the procedure from part II (respiration) find the plant respiration for 15, 20, 30, and 35 degrees.

Temperature (C) Trial 1 DO Difference Trial 2 DO Difference Trial 3 DO Difference Average

(Respiration/Hour)

15 .12 .1259 .1262 .12403

20 .1774 .1704 .1753 .5231

30 .1354 .1402 .1412 .4168

35 .0675 0.0719 .0653 .06823

3. Set the fish number to 0 and the plant number to 1. Leave the other variables at the default values
(100% light intensity, white light).
4. Set the temperature to 15 degrees.
5. Record the starting and ending oxygen values.
6. Repeat steps 3 and 4 for 20, 25, 30, and 35 degrees.
7. Calculate and record the DO difference, Oxygen (O2) production (in 60 minutes), and Oxygen production
per minute. For respiration, use the value determined for each temperature.

Temperature (C) DO start DO end DO Difference O2 Production* O2 Production/min

15 7.4766 7.7069 -0.2303 1.01 0.6183

20 7.2754 7.6328 -0.3754 .1477 0.0022461

25 7.0459 7.4392 -0.3933 -0.19704 -0.003284

30 6.5949 6.8694 -0.2745 .1423 0.0023716

35 6.0625 6.1772 -0.1147 -0.4647 0.0007783

*O2 Production = DO Difference + Respiration

8. Insert a graph displaying how temperature affects O2 production per minute.

9. Write a sentence conclusion.

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In conclusion, I found significant differences showing that increasing temperature increase oxygen production.
Reinhardtii production significantly more oxygen than the mutant 15°C and 27°C.

10. Write a paragraph explaining your conclusion in terms of the process of photosynthesis.

Based on statistical analysis, the results from the simulation showed that the degree at 35°C produced
significantly at 25. At the degree, it still produced more oxygen than 20-15

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