Unit 1: Cell and reproduction

Laboratory Practice 3: “Asexual reproduction”

[Delivery date]

Professor: MD. in ECh. Miguel Alberto López Sánchez

[Student’s full name]
[Student’s full name]
[Student’s full name]
[Student’s full name]
Subject: Biology II
Semester: 4th. Group:
Bicultural Highschool
Lab practice 2. Mitosis
Objective
The student will establish the different phases of mitosis and its relationship to asexual
reproduction.

Hypothesis

Previous knowledge

1. What is asexual reproduction?

2. What is the importance of reproduction in living things?

3. Describe the types of asexual reproduction

INTRODUCTION
[Write a brief introduction of the main topic]

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MATERIALS AND SUBSTANCES

Material provided by Material provided by

Qty. Materials
the laboratory the student
1 Plastic Pasteur pipette *
1 Dissection needle *
1 Slide *
1 Coverslip *
1 Microscope *
1 Inoculation loop *
Biological material
Tortilla/bread with mold* *
Yeast *
Aloe vera plant with pups *
A piece of sud *
*Wash your hands thoroughly after touching mold samples.

PROCEDURE
Budding process in yeast
1. Place the sample on a slide and add a drop of water using the inoculation loop.
2. Mix and heat the slide gently using a match to stimulate yeast reproduction. Do not
overheat the slide.
3. Look at the sample under the microscope using the 10x lens.
4. Locate yeasts in budding.
5. Look at the sample under the microscope with the 40x lens for a few minutes.

Spore formation
1. Place a sample of the mold from the tortilla or bread on a slide using the inoculation loop.
2. Observe the sample under the microscope using the 10x and 40x lenses.
3. Draw all the structures you found.

Plant propagation
1. Observe the pups from the aloe vera plant. Draw your observations.
2. Observe the stems from the suds and identify the rhizomes. Draw your observations.

PROCEDURE FLOWCHART

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OBSERVATIONS
Draw the organisms in asexual reproduction. Indicate the names of the structures observed.

Yeast Mold

Aloe vera plant Sud

RESULTS AND DISCUSSIONS

Complete the following chart. Indicate with an “x” the type of reproduction observed in each
organism. Then, discuss the results found.

Spore formation Stolon Budding Sprouts

Yeast
Mold
Aloe vera
Sud

QUIZ
1. What would be the biological advantage of asexual reproduction?

2. Give five examples of organisms that can asexually reproduce and the name of the type

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3. How do farmers take advantage of plant propagation?

4. What is the relation between mitosis and asexual reproduction?

CONCLUSIONS

REFERENCES

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