Unit 1: Cell and reproduction

Laboratory Practice 4: “Sexual reproduction”

[Delivery date]

Professor: MD. in ECh. Miguel Alberto López Sánchez

[Student’s full name]
[Student’s full name]
[Student’s full name]
[Student’s full name]
Subject: Biology II
Semester: 4th. Group:
Bicultural Highschool
Lab practice 4. Sexual reproduction
Objective
The student will distinguish the types of gametes and appropriately correlate their role in the
sexual reproduction process.

Hypothesis
distinguish the types of gametes and appropriately correlate their role in the sexual reproduction
process. through practice where we will observe and analyze the sexual reproduction of a flower

Previous knowledge

1. What is sexual reproduction?

the production of new living organisms by combining genetic information from two individuals of
different types (sexes). In most higher organisms, one sex (male) produces a small motile gamete
which travels to fuse with a larger stationary gamete produced by the other (female).

2. What are gametes?

a reproductive cell of an animal or plant

3. What is the role of gametes in sexual reproduction?

reproductive cells that unite during sexual reproduction to form a new cell called a zygote

INTRODUCTION
[Write a brief introduction of the main topic]

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MATERIALS AND SUBSTANCES

Material provided by Material provided by

Qty. Materials
the laboratory the student
1 Plastic Pasteur pipette *
1 Dissection needle *
2 Slide *
2 Coverslip *
1 Microscope *
1 Scalpel *
Biological material
1 Tulip or rose *
*Wash your hands thoroughly after the practice.

PROCEDURE
Male gametes observation
1. Look carefully at the flower and its receptacle.
2. Carefully remove the sepals.
3. Remove the petals without damaging the internal structures and identify them.
4. Place an anther on a slide and add a droplet of water.
5. Cover the sample with a coverslip and carefully crush it.
6. Observe under a light microscope at 10x and 40x. Draw your observations.

Female gametes observation

1. Make a cross section of the ovary.
2. Place the section on a slide, adding a drop of water.
3. Place the coverslip avoiding the formation of bubbles and observe under a light
microscope. Make your drawings.

PROCEDURE FLOWCHART

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OBSERVATIONS
Draw the pollen grains and the cross section of the ovary.

Pollen grains Ovary cross section

Objective lens: Objective lens:

RESULTS AND DISCUSSIONS

Discuss the results found.

QUIZ
1. Where are the sperms cells located in the flower?
A stamen consists of an anther (which produces pollen, the male reproductive cell) and a filament.

2. Where are the ova located in the flower?

beneath or above, or at the point of connection with the bottom of sepals and petals

3. What does the term haploid mean?

the presence of a single set of chromosomes in an organism's cells

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4. What are the gametes of the flower?
male and female gametes

5. Why do gametes need to be haploid cells?

because they will be combining with another gamete

CONCLUSIONS
The life cycles of plants are very different from those of animals and humans.

In sexual cycles of plants there are two phases or multicellular forms, the sporophyte, made up of
diploid spore forming cells, and the gametophyte of haploid cells and gamete forming plant sexual
cycles involve alternation of generations.

REFERENCES
Sexual reproduction in Plants and Animals: Stages, Examples, Videos etc. (2019,
September 17). Toppr-
guides. https://www.toppr.com/guides/biology/reproduction-in-organisms/
sexual-reproduction/

V. (n.d.). Sexual Reproduction in Plants.

VEDANTU. https://www.vedantu.com/biology/sexual-reproduction-in-plants

YR 9: Topic 7: Reproduction in Plants. (n.d.). AMAZING WORLD OF SCIENCE WITH

MR. GREEN. https://www.mrgscience.com/yr-9-topic-7-reproduction-in-
plants.html

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