CER135 M1 - Fundamanetals - Micros

MICROSCOPY AND X-RAY CHARACTERIZATION
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CER135
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G I L WU
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Fundamentals of Microscopy
Module 1
(Slides from Dr.Ivyleen C. Bernardo-Arugay)
1
MICROSCOPY AND X-RAY
I C E
CHARACTERIZATION
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C CER135
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G
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L Minimum preparations (12 units of graduate courses)
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3-UNIT GRAD COURSE ON ELECTRON MICROSCOPY
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1-UNIT GRAD COURSE ON SCANNING ELECTRON MICROSCOPY
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LABORATORY
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3-UNIT GRAD COURSE ON X-RAY CRYSTALLOGRAPHY AND
E O SPECTROSCOPY
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M N
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3-UNIT GRAD COURSE ON XRD AND THE IDENTIFICATION OF CLAY
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A MINERALS & OTHER CLAY-SIZED MATERIALS
B
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I 1-UNIT GRAD COURSE ON CRYSTALLOGRAPHY LABORATORY
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1-UNIT GRAD COURSE ON METALLOGRAPHY
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Learning Objectives
FuNDAMENTAlS OF MICROSCOPY CER135 Module 1
G.A. #1� Group Activity #1-Illustration of Microscopes
I C E
CER135-1_IllustrationMicroscope_family name
N O X DUE ON OCTOBER 5,2022 3:00PM
T M C
E M E
G I L GROUP 1:
R T L GROUP 2:
I M E
T E N
GROUP 3:
Y N C GROUP 4:
T E GROUP 5:
A
C
GROUP 6:
T C GROUP 7:
E O
A U
GROUP 8:
M N
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K L
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Learning Objectives
G.A. #1� Group Activity #1-Illustration of Microscopes
I C E
CER135-1_IllustrationMicroscope_family name
N O X DUE ON OCTOBER 5,2022 3:00PM
T
E
M
M
C
E Illustrate a typical Optical microscope and a typical Electron
G I L microscope. Label essential parts and their respective functions .
R T L OBSERVE PROPER CITATION.
I M E
T E N
Y N C
T E
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T C
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FuNDAMENTAlS OF MICROSCOPY CER135 Module 1 G.A.1
Criteria Description almost above fair poor

complete satisfactory (essential
I C E information (clear information (no
N O X (clear presentation are answer,
T M C presentation of essential available) plagiarise
E M E of important information d answer
G I L
information) are available)
R T L
I M E score counts 10-9 8-7 6-5 0
T E N
Y N C Sketch of a Illustration of a typical Optical
T E OM 10% microscope.
A
C OM parts 15% Important parts for image
T C production are identified.
E O
A U Sketch of a Illustration of a typical Scanning
M N
T
EM 10% Electron Microscope.
W
O
A EM parts 15% Important parts for image
B
R I production are identified
K L
I Citation 10% Proper citation of references
Y
FuNDAMENTAlS OF MICROSCOPY CER135 Module 1-G.A.1

T M C presentatio of essential available) plagiarise
E M E n of information d answer
G I L
important are available)
R T L
I M E information)
T E N
Y N C
score counts 10-9 8-7 6-5 0
T E Principle of Optical definition of fundamental
A Microscopy A 10% principles in optical microscopy
C
T C
Principle of Optical illustration of fundamental
E O Microscopy B 10% principles in optical microscopy
A U
N Principle of Electron definition of fundamental
M
W T Microscopy A 10% principles in electron
A microscopy
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B
R I Principle of Electron illustration of fundamental
K L Microscopy B 10% principles in electron
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T microscopy IVYLEEN C. BERNARDO-ARUGAY 6
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G.A. 1 -3. due 2022OCT05.WED. 3:00PM. REMEMBER ICETA always.

I C E
N O X • CER135-1_IllustrationMicroscope_family name
T M C
E M E • CER135-2_CnContrastMicroscopes_family name
G I L
R T L • CER135-3_SemTem_family name (no due date yet)
I M E
T E N • Each GA will be accomplished on a 5-slide powerpoint presentation
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(including references). Upload to your assigned google folder. Make
A
sure all essential details are legible.
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• Observe proper citation. Place the reference on the 5th slide of the
E O powerpoint presentation.
A U
M N
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• Observe proper filename of your submission and upload your G.A. on
W
O
A your prescribed google folder. ALWAYS have your family name/s
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B
I
appear on the footer of the slides
K L
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Optical Microscopy: Illustration of the Principle

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G.A.#1. EXPECTATIONS
T M C
E M E • Illustrate how is light is being harnessed in optical microscopy.
G I L
R T L • Show and label essential parts of the optical microscopes in
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N harnessing light in optical microscopy
Y N C
T E • What are the roles of the essential parts of the optical microscopes?
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I C E
OPTICAL MICROSCOPE ELECTRON MICROSCOPE
N O X
T M C • Dutch spectacles makers • In 1931 physicist Ernst Ruska and
E M E Zacharius Jansen and his father German engineer Max Knoll.
G
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L Hans were the first to invent the
I M E compound microscope in the • Complicated operating system
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16th century. • Users require technical skills
T E • Less complex operating system • Very expensive to buy as well as to
A maintain.
C • Simple to use
T C • Expensive replacement parts
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• Cheap to buy and has low • 2D only in a Transmission electron
M N maintenance costs. microscope (TEM);
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A • Cheap replacement parts Scanning electron microscope
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B (SEM) images give depth
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• Image plane “flat” (2D). information which seems like 3D.
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OPTICAL MICROSCOPE ELECTRON MICROSCOPE
N O X • Living processes cannot be viewed
T M C • Visualization of living processes
E M E such as microscopic pond life in • Risk of radiation leakage.
G
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L action and even cell division is • Long period of training and highly
I M E possible. skilled
T E N
Y N C • No radiation risk. • Discipline-inclined
T E
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• Short and above average skill • It is used in the study of the external
surface, the ultrastructure of cell and
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C HS students very small organisms.
E O
A U It is used for the study of detailed • It is used in the study of external and
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gross internal structure. shallow surfaces of inorganic materials
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A ex. Depth, size, PSD, morphology
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I • It can also include elemental analysis on
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I the surface of materials ex. EDS/EDX
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Optical Microscopy:
I C E Definition &
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Principle
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• ILLUMINATING SOURCE:
T E N • Uses light (400-700
Y N C
T E
nm) to illuminate the
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objects under view in
C an optical microscope
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M N • Image is formed by
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absorption of light waves.
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K Fig. 1. Scheme of a light microscope.
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Optical Microscopy: Typical Optical Microscopes

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N O X • Light microscopes are smaller and weighs lighter than Electron Microscopes
T M C
E M E
G.A. #2 Compare and Contrast (primary common & primary unique)
G I L CER135-2_CnContrastMicroscopes_family name
R T L
I M E DUE ON OCTOBER 5 at 3:00PM
T E N
Y N C • Dark-field microscope
T E
• Phase-contrast microscope
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C • Fluorescent microscope
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O Confocal microscope
A U Polarized microscope
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W T • Differential interference contrast microscope
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FuNDAMENTAlS OF MICROSCOPY CER135 Module 1-G.A.2

G I L
R T L
I M E information)
T E N
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T E COMMON IDENTIFY PRIMARY SIMILARITIES
A FEATURES OF THE OF THE MICROSCOPES
C MICROSCOPES
T C
E O 18%
A U DARK-FIELD CORRESPONDING UNIQUE
M N
T MICROSCOPE 12% FEATURES
W
A PHASE-CONTRAST CORRESPONDING UNIQUE
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B
R I MICROSCOPE 12% FEATURES
K L FLUORESCENT CORRESPONDING UNIQUE
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T MICROSCOPE 12% FEATURES IVYLEEN C. BERNARDO-ARUGAY 13
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FuNDAMENTAlS OF MICROSCOPY CER135 Module 1 S.A.2

T M C presentation of essential available) plagiarise
E M E of important information d answer
G I L
information) are available)
R T L
I M E score counts 10-9 8-7 6-5 0
T E N
Y N C CONFOCAL MICROSCOPE CORRESPONDING
T E 12% UNIQUE FEATURES
A
C POLARIZED MICROSCOPE CORRESPONDING
T C 12% UNIQUE FEATURES
E O
A U DIFFERENTIAL CORRESPONDING
M N
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INTERFERENCE CONTRAST UNIQUE FEATURES
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A MICROSCOPE 12 %
B
R I Citation 10% Proper citation of
K L references
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Electron Microscopy: Types of Electron
I C E Microscopes S.A. #3 (cite 3 major differences)
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CER135-3_SemTem_family name
G I L Scanning electron microscope (SEM) Transmission electron microscope (TEM)
R T L
I M E
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T E
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T C
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Electron
I C E Microscopy:Illustration
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C of the Principle
E M E
G I L • ILLUMINATING SOURCE:
R T L
I M E • Uses a beam of
T
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E
N
N
C
electrons (approx
T E equivalent wavelength
A 1 nm) to make objects
C
T C larger for a detailed
E
A
O
U view.
M N
W T • Image is formed by
A
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B scattering or transmission
K
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L of electrons.
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FuNDAMENTAlS OF MICROSCOPY CER135 Module 1-S.A.3

G I L
R T L
I M E information)
T E N
Y N C
T E 1ST MAJOR SAMPLE PREPARATION
A DIFFERENCE 30%
C
T C 2ND MAJOR INFORMATION UNIQUE FROM
E O
A U
DIFFERENCE 30% SEM & TEM
M N 3RD MAJOR INFORMATION UNIQUE FROM
W T
A DIFFERENCE 30% SEM & TEM
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R I Citation 10% Proper citation of references
K L
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FuNDAMENTAlS OF MICROSCOPY CER135 Module 1 ARCHIVE
S.A. 1 -3. due 2022OCT5.WED. 6:10PM after CER135 AD HOC SESSION

I C E
REMEMBER ICETA always.
N O X • CER135-1_IllustrationMicroscope_family name
T M C
E M E • CER135-2_CaContrastMicroscopes_family name
G
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• CER135-3_SemTem_family name
I M E • Each SA will be accomplished EITHER on a 1-page short bond paper (for
T E N those who does not have free access to computer) OR on a 3-slide
Y N C powerpoint presentation (for those who have free access to computer). For
T E those who will be submitting the 1-page short bond paper just take a clear
A picture of your work and upload to your assigned google folder. Make sure
T
C
C
all essential details are legible.
E
A
O • Observe proper citation. Place the reference at the bottom of the 1-page
M
U
N short bond paper OR on the 4th slide of the powerpoint presentation.
W T
A • Observe proper filename of your submission and upload your S.A. on your
O
B prescribed google folder. ALWAYS include your name on top portion of the
R
K
I paper OR have your name appear on the footer of the slides
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MICROSCOPY AND X-RAY CHARACTERIZATION

Criteria Description almost above fair poor

Criteria Description almost above fair poor

G.A. 1 -3. due 2022OCT05.WED. 3:00PM. REMEMBER ICETA always.

Optical Microscopy: Illustration of the Principle

Optical Microscopy: Typical Optical Microscopes

Criteria Description almost above fair poor

Criteria Description almost above fair poor

Criteria Description almost above fair poor

S.A. 1 -3. due 2022OCT5.WED. 6:10PM after CER135 AD HOC SESSION

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