SYNTHESIS OF IRON OXIDE NANOPARTICLES USING

LAWSONIA INERMIS EXTRACT

https://doi.org/10.1007/s12668-019-00665-5

Plant used - L. inermis (Henna)

Plant part leaves – Leaves

NP synthesized - Iron Oxide Nanoparticles

Materials used - Fresh leaves of L. inermis (Henna), Iron sulphate (FeSO 4), Sodium hydroxide (NaOH) [All
the reagents were of analytical grade]

Preparation of Plant Extract from Leaves

 Thoroughly wash fresh leaves of L. inermis (henna). Rinse several times with distilled water to
remove dirt particles.
1
 Washed leaves are ground in deionized water in the kitchen grinder (w/v, 1.0 g/10.0 ml) .
 Extract sieved through muslin cloth.
 Centrifuge to remove leaf debris present in the extract.

1 - "w/v" stands for weight/volume ratio, "1.0 g/10.0 ml" means that, while grinding 1.0 gram of the ingredient should be used
for every 10.0 milliliters of volume.

Preparation of Iron Oxide Nanoparticles

FeSO4 is used as the precursor salt for the synthesis of Iron Oxide NPs. Henna leaf extract served as the
natural reducing and capping/stabilizing agent.

 A 10 millimolar FeSO4 solution is prepared by dissolving it in 50 mL of deionized water.

 Henna extract and the salt were mixed in a 1:1 volumetric ratio.
 The pH of the mixture is adjusted to 11 using a 1 N NaOH solution.
 The synthesis mixture is incubated at 80 °C in a water bath for 30 minutes.
 After incubation, the solution is cooled to room temperature.
 The solution is then centrifuged at 10,000 rpm at room temperature.
 The resulting pellet containing the nanoparticles is washed multiple times using water and
methanol.
 The washed nanoparticles were dried at 50 °C in an oven.
SYNTHESIS OF TITANIUM DIOXIDE NANOPARTICLES
USING AZADIRACHTA INDICA EXTRACT
https://doi.org/10.1016/j.sajb.2019.05.024

Krishnasamy et al. (2015)

Plant used - Azadirachta indica (Neem)

Plant part leaves – Leaves

NP synthesized - TiO2 Nanoparticles

Materials used - Fresh leaves of Azadirachta indica (Neem), aqueous TiO2

Preparation of Plant Extract from Leaves

 20 g of finely cut fresh Azadirachta indica leaves are used.
 The leaves are washed thoroughly with distilled water.
 The washed leaves boiled in 100 mL of sterile double distilled water for 30 min using a water bath.
 After boiling, the resulting extract is filtered to remove any particulate matter.
 The filtered extract is then stored at 4 °C for future use.

Preparation of Titanium Oxide Nanoparticles

 10 mL of Azadirachta indica leaf broth (prepared above) is added to 90 mL of 5 mM aqueous TiO 2.
The purpose of this addition is to synthesize titanium nanoparticles. (Both the papers referred just
mentions the leaf broth to be added to aq. TiO 2 soln. no intermediate steps or specific conditions were
specified.)

 The resulting mixture is purified through repeated centrifugation.

 Centrifugation performed at 11,000 rpm for 15 minutes. Double distilled water used for the
centrifugation process.

 After centrifugation, the pellet containing the titanium nanoparticles is obtained.

 The pellet is then re-dispersed in deionized water.