EXTRACTION PROCEDURE :

Essential Oil Isolation from Buds, Leaves and Stems

The dried plant materials of buds, leaves (1.0 kg) were
treated by steam-distillation method by conventional steam distiller, for 6 hours.
The essential oil samples were dried by anhydrous sodium sulfate (Na 2SO4) and
stored in cool until further analysis conducted.

Miswak samples
The sticks were dried in a 55◦C oven for three days and then sliced or ground into a fine powder using a
coffee grinder .Miswak extracts were prepared by adding 40 g of miswak powder to 200 ml of solvent
(water, ethanol and hexane) in a closed container and soaked at room temperature for 48 h. The solvents
were filtrated through a Whatman No. 1 filter paper and allowed to evaporate in a40 ◦C oven for 72 h.

OR

20 Gram of miswak was added into chloroform ,acetone, ethanol and the extraction was carried by

1. Maceration at room temperature

2. Continuous hot extraction using Soxhlet apparatus

Preparation of extracts of rosemarry

Aqueous extract (AE) of sage was obtained
by extraction of crushed dried leaves (10 g, <710 μm)
with water at 80 °C (100 mL) for 15 min. The mixture
was filtered (Whatman nº 1), and the extract volume
completed to 100 mL. Fluid extract (FE) was prepared
by successive percolations of crushed dried leaves of
sage (20 g, <710 μm) with 56% ethanol (v/v) for three
days, in the dark, at room temperature (Farm Bras, 2002).
The extracts were combined and concentrated to 20 mL.
Samples were sterilized by filtration through a 0.22 μm
membrane filter and stored at -25 °C till use.