"""""""""""""""""""""""""""""""""""Secuencia nativa de la proteína """"""""""""""""""""""""""""""""""

ATG-GAG-CAC-ACA-CAC-TCA-ACG-CTC-CTC-CCC-CGC-ATG-GCC-GGC-ATG-GAT-ATG-TCC-
TCG-TCA-GCG-ACT-AAT-GCT-TCC-TGC-TCT-GTC-GAG-ATG-CTC-TGG-AAC-TGG-AAA-GTG-
ATC-GAC-TCA-TGC-TTT-CTT-TCA-TCG-ACA-TGG-CAC-GTC-ACT-ACA-AAA-GGC-ATG-TTT-
GCC-GTC-TCC-TGC-ATC-GGC-GTT-GCG-CTC-CTG-GGG-GTC-GCG-CTC-GAG-TTC-CTG-CGG-
CGC-GTA-TCC-AAG-GAT-TAC-GAA-GAG-AGC-CTG-CAG-CGG-CAG-TTT-CAG-CGG-CAT-GCG-
ATT-GCG-CAG-CTT-GAC-GAC-CCG-CTG-GTG-TGC-GGT-GTG-CCC-GCC-AGT-GTG-GTC-ACC-
TAC-CGA-GCG-AGC-CCG-CTG-CAG-CAA-ATT-ATC-CGG-GCG-GTA-CTG-CAT-GTA-GCG-CAG-
TTC-GGG-GTG-GCA-TAT-ATC-ACC-ATG-CTG-ATC-GCG-ATG-TAT-TAC-AAC-GGG-TAC-ATG-
ATC-ATC-TCG-ATC-TTT-ATC-GGT-GCG-TTC-TTG-GGC-AAA-CTC-TTC-TTC-GAC-TGG-GGG-
CAA-TAT-AAG-GTC-GTG-CTG-GGG-CAG-ACG-CAG-GGA-CCT-GCA-GCG-GCT-AAG-GTC-GAG-
GAG-GAA-GCG-ACC-AAG-TGT-TGT-GGC-TAG

M-E-H-T-H-S-T-L-L-P-R-M-A-G-M-D-M-S-S-S-A-T-N-A-S-C-S-V-E-M-L-W-N-W-K-V-I-D-S-C-F-L-S-S-
T-W-H-V-T-T-K-G-M-F-A-V-S-C-I-G-V-A-L-L-G-V-A-L-E-F-L-R-R-V-S-K-D-Y-E-E-S-L-Q-R-Q-F-Q-R-
H-A-I-A-Q-L-D-D-P-L-V-C-G-V-P-A-S-V-V-T-Y-R-A-S-P-L-Q-Q-I-I-R-A-V-L-H-V-A-Q-F-G-V-A-Y-I-T-M-
L-I-A-M-Y-Y-N-G-Y-M-I-I-S-I-F-I-G-A-F-L-G-K-L-F-F-D-W-G-Q-Y-K-V-V-L-G-Q-T-Q-G-P-A-A-A-K-V-E-
-E-E-A-T-K-C-C-G
“”""""""""""""CASO A: Modificar las 5 primeras metioninas por alaninas) ”””"""""""""""""”””””””””””

ATG Iniciacion-GAG-CAC-ACA-CAC-TCA-ACG-CTC-CTC-CCC-CGC-GCC-GCC-GGC-GCC-GAT-GCC-
TCC-TCG-TCA-GCG-ACT-AAT-GCT-TCC-TGC-TCT-GTC-GAG-ATG-CTC-TGG-AAC-TGG-AAA-
GTG-ATC-GAC-TCA-TGC-TTT-CTT-TCA-TCG-ACA-TGG-CAC-GTC-ACT-ACA-AAA-GGC-ATG-
TTT-GCC-GTC-TCC-TGC-ATC-GGC-GTT-GCG-CTC-CTG-GGG-GTC-GCG-CTC-GAG-TTC-CTG-
CGG-CGC-GTA-TCC-AAG-GAT-TAC-GAA-GAG-AGC-CTG-CAG-CGG-CAG-TTT-CAG-CGG-CAT-
GCG-ATT-GCG-CAG-CTT-GAC-GAC-CCG-CTG-GTG-TGC-GGT-GTG-CCC-GCC-AGT-GTG-GTC-
ACC-TAC-CGA-GCG-AGC-CCG-CTG-CAG-CAA-ATT-ATC-CGG-GCG-GTA-CTG-CAT-GTA-GCG-
CAG-TTC-GGG-GTG-GCA-TAT-ATC-ACC-ATG-CTG-ATC-GCG-ATG-TAT-TAC-AAC-GGG-TAC-
ATG-ATC-ATC-TCG-ATC-TTT-ATC-GGT-GCG-TTC-TTG-GGC-AAA-CTC-TTC-TTC-GAC-TGG-
GGG-CAA-TAT-AAG-GTC-GTG-CTG-GGG-CAG-ACG-CAG-GGA-CCT-GCA-GCG-GCT-AAG-GTC-
GAG-GAG-GAA-GCG-ACC-AAG-TGT-TGT-GGC-TAG Terminación

El primer codon creo que no debería cambiarse ya que es el triplete de iniciación ¿no impediría la
transcripción a RNAm?

A-E-H-T-H-S-T-L-L-P-R-A-A-G-A-D-A-S-S-S-A-T-N-A-S-C-S-V-E-M-L-W-N-W-K-V-I-D-S-C-F-L-S-S-
T-W-H-V-T-T-K-G-M-F-A-V-S-C-I-G-V-A-L-L-G-V-A-L-E-F-L-R-R-V-S-K-D-Y-E-E-S-L-Q-R-Q-F-Q-R-
H-A-I-A-Q-L-D-D-P-L-V-C-G-V-P-A-S-V-V-T-Y-R-A-S-P-L-Q-Q-I-I-R-A-V-L-H-V-A-Q-F-G-V-A-Y-I-T-M-
L-I-A-M-Y-Y-N-G-Y-M-I-I-S-I-F-I-G-A-F-L-G-K-L-F-F-D-W-G-Q-Y-K-V-V-L-G-Q-T-Q-G-P-A-A-A-K-V-E-
-E-E-A-T-K-C-C-G

Código (1 letra) Código (3 letras) Aminoácido

A Ala Alanina
R Arg Arginina
N Asn Asparagina
D Asp Aspartato
C Cys Cisteína
Q Gln Glutamina
E Glu Ácido glutámico
G Gly Glicina
H His Histidina
I Ile Isoleucina
L Leu Leucina
K Lys Lisina
M Met Metionina
F Phe Fenilalanina
P Pro Prolina
S Ser Serina
T Thr Treonina
W Trp Triptófano
Y Tyr Tirosina
 V Val Valina
“”""""”””""""""CASO B: Delección de la secuencia polipeptídica indicada ”””"""”""””””””””””

ATG Iniciacion-GAG-CAC-ACA-CAC-TCA-ACG-CTC-CTC-CCC-CGC-ATG-GCC-GGC-ATG-GAT-ATG-
TCC-TCG-TCA-GCG-ACT-AAT-GCT-TCC-TGC-TCT-GTC-GAG-ATG-CTC-TGG-AAC-TGG-AAA-
GTG-ATC-GAC-TCA-TGC-TTT-CTT-TCA-TCG-ACA-TGG-CAC-GTC-ACT-ACA-AAA-GGC-ATG-
TTT-GCC-GTC-TCC-TGC-ATC-GGC-GTT-GCG-CTC-CTG-GGG-GTC-GCG-CTC-GAG-TTC-CTG-
CGG-CGC-GTA-TCC-AAG-GAT-TAC-GAA-GAG-AGC-CTG-CAG-CGG-CAG-TTT-CAG-CGG-CAT-
GCG-ATT-GCG-CAG-CTT-GAC-GAC-CCG-CTG-GTG-TGC-GGT-GTG-CCC-GCC-AGT-GTG-GTC-
ACC-TAC-CGA-GCG-AGC-CCG-CTG-CAG-CAA-ATT-ATC-CGG-GCG-GTA-CTG-CAT-GTA-GCG-
CAG-TTC-GGG-GTG-GCA-TAT-ATC-ACC-ATG-CTG-ATC-GCG-ATG-TAT-TAC-AAC-GGG-TAC-
ATG-ATC-ATC-TCG-ATC-TTT-ATC-GGT-GCG-TTC-TTG-GGC-AAA-CTC-TTC-TTC-GAC-TGG-
GGG-CAA-TAT-AAG-GTC-GTG-CTG-GGG-CAG-ACG-CAG-GGA-CCT-GCA-GCG-GCT-AAG-GTC-
GAG-GAG-GAA-GCG-ACC-AAG-TGT-TGT-GGC- TAG Terminación

M-E-H-T-H-S-T-L-L-P-R-M-A-G-M-D-M-S-S-S-A-T-N-A-S-C-S-V-E-M-L-W-N-W-K-V-I-D-S-C-F-L-S-S-
T-W-H-V-T-T-K-G-M-F-A-V-S-C-I-G-V-A-L-L-G-V-A-L-E-F-L-R-R-V-S-K-D-Y-E-E-S-L-Q-R-Q-F-Q-R-
H-A-I-A-Q-L-D-D-P-L-V-C-G-V-P-A-S-V-V-T-Y-R-A-S-P-L-Q-Q-I-I-R-A-V-L-H-V-A-Q-F-G-V-A-Y-I-T-M-
L-I-A-M-Y-Y-N-G-Y-M-I-I-S-I-F-I-G-A-F-L-G-K-L-F-F-D-W-G-Q-Y-K-V-V-L-G-Q-T-Q-G-P-A-A-A-K-V-E-
E-E-A-T-K-C-C-G

Código (1 letra) Código (3 letras) Aminoácido

A Ala Alanina
R Arg Arginina
N Asn Asparagina
D Asp Aspartato
C Cys Cisteína
Q Gln Glutamina
E Glu Ácido glutámico
G Gly Glicina
H His Histidina
I Ile Isoleucina
L Leu Leucina
K Lys Lisina
M Met Metionina
F Phe Fenilalanina
P Pro Prolina
S Ser Serina
T Thr Treonina
W Trp Triptófano
 Y Tyr Tirosina
V Val Valina
“""""""""""”""""""""""""""""""""" CASO C: Inserción de la secuencia polipeptídica indicada """""""""""”"”””""""""""""""""""""
ATG Iniciacion-GAG-CAC-ACA-CAC-TCA-ACG-CTC-CTC-CCC-CGC-ATG-GCC-GGC-ATG-GAT-ATG-
TCC-TCG-TCA-GCG-ACT-AAT-GCT-TCC-TGC-TCT-GTC-GAG-ATG-CTC-TGG-AAC-TGG-AAA-
GTG-ATC-GAC-TCA-TGC-TTT-CTT-TCA-TCG-ACA-TGG-CAC-GTC-ACT-ACA-AAA-GGC-ATG-
TTT-GCC-GTC-TCC-TGC-ATC-GGC-GTT-GCG-CTC-CTG-GGG-GTC-GCG-CTC-GAG-TTC-CTG-
CGG-CGC-GTA-TCC-AAG-GAT-TAC-GAA-GAG-AGC-CTG-CAG-CGG-CAG-TTT-CAG-CGG-CAT-
GCG-ATT- GCG-CAG-CTT-GAC-ATT-CAG-TGT-CGT-GAC-CCG-CTG-GTG-TGC-GGT-GTG-CCC-
GCC-AGT-GTG-GTC-ACC-TAC-CGA-GCG-AGC-CCG-CTG-CAG-CAA-ATT-ATC-CGG-GCG-GTA-
CTG-CAT-GTA-GCG-CAG-TTC-GGG-GTG-GCA-TAT-ATC-ACC-ATG-CTG-ATC-GCG-ATG-TAT-
TAC-AAC-GGG-TAC-ATG-ATC-ATC-TCG-ATC-TTT-ATC-GGT-GCG-TTC-TTG-GGC-AAA-CTC-
TTC-TTC-GAC-TGG-GGG-CAA-TAT-AAG-GTC-GTG-CTG-GGG-CAG-ACG-CAG-GGA-CCT-GCA-
GCG-GCT-AAG-GTC-GAG-GAG-GAA-GCG-ACC-AAG-TGT-TGT-GGC- TAG Terminación

M-E-H-T-H-S-T-L-L-P-R-M-A-G-M-D-M-S-S-S-A-T-N-A-S-C-S-V-E-M-L-W-N-W-K-V-I-D-S-C-F-L-S-S-
T-W-H-V-T-T-K-G-M-F-A-V-S-C-I-G-V-A-L-L-G-V-A-L-E-F-L-R-R-V-S-K-D-Y-E-E-S-L-Q-R-Q-F-Q-R-
H-A-I-A-Q-L-D-I-Q-C-R-D-P-L-V-C-G-V-P-A-S-V-V-T-Y-R-A-S-P-L-Q-Q-I-I-R-A-V-L-H-V-A-Q-F-G-V-
A-Y-I-T-M-L-I-A-M-Y-Y-N-G-Y-M-I-I-S-I-F-I-G-A-F-L-G-K-L-F-F-D-W-G-Q-Y-K-V-V-L-G-Q-T-Q-G-P-A-
A-A-K-V-E-E-E-A-T-K-C-C-G

Código (1 letra) Código (3 letras) Aminoácido

A Ala Alanina
R Arg Arginina
N Asn Asparagina
D Asp Aspartato
C Cys Cisteína
Q Gln Glutamina
E Glu Ácido glutámico
G Gly Glicina
H His Histidina
I Ile Isoleucina
L Leu Leucina
K Lys Lisina
M Met Metionina
F Phe Fenilalanina
P Pro Prolina
S Ser Serina
T Thr Treonina
W Trp Triptófano
Y Tyr Tirosina
V Val Valina