EFFICACY OF OREGANO LEAVES AND AFRICAN TALISAY LEAVES AGAINST

MOSQUITO

RESEARCHER PROPONENTS:

PEARL ZOE P. GENCIANOS

JOYELLE N. GALLARDO

GIANNINA FAYE C. BARRIENTOS

JANNA CARLA DE GUZMAN

ARHIANA NICOLE P. LLANOS

FRANCIS DAVE L. TRESPLACIOS

JOSHUA N. LANTINGAN

JAYMAR QUITOR
PROJECT QUESTION:

Is Oregano leaves and African Talisay leave extracts effective as an alternative mosquito
repellent in helping us avoid and lower the case of Dengue?

INTRODUCTION

Dengue is one of the most alarming diseases that if faced globally and nationally.

Dengue is a mosquito-borne illness that occurs in tropical and subtropical areas of the world.

It has remained an endemic disease ever since the first epidemic in Southeast Asia was

documented in Manila 1954. The viral illness dengue, which is spread by mosquitoes, has

recently spread quickly throughout all WHO areas. Female mosquitoes, primarily of the

species Aedes aegypti and, to a lesser extent, Ae, carry the dengue virus. Albopictus. These

mosquitoes can transmit the Zika, yellow fever, and chikunguya viruses. The risk of dengue

varies locally depending on local climate, socioeconomic, and environmental factors, which

are both common in tropics.

Dengue is endemic in more than 100 countries in Asia, the Pacific, the Americas,

Africa, and the Caribbean. Each year, up to 400 Million people get infected with dengue

globally. Up to 100 Million gets infected severely, and up to 40,00 people die from the said

disease. According to the World Health Organization (WHO), there were 4.2 million reported

cases of dengue in the Americas in 2019, with 1,489 deaths. In the Western Pacific region,

there were 1.2 million reported cases of dengue in 2019, with 1,254 deaths. Southeast Asia

has the highest burden of dengue in the world, with an estimated 67 million cases per year. In

our country, an increase of 191% from the 67,357 cases reported during the same period in

2021 to 196,728 cases and 642 deaths between 1 January and 5 November 2022(Department

of Health, 2022).
 Currently, there is no treatment for dengue. But a vaccine was made that was

supposed to help prevent dengue from spreading. Dengvaxia was the name of the vaccine and

it was given to people ages 6 to 45 who had previously got the dengue virus (EUROPEAN

MEDICINES AGENCY, 2022). It was created by Sanofi Pasteur inc., a division of the

French pharmaceutical. They created the vaccine using recombinant DNA technology and

substituted homologous sequences from the four dengue virus serotypes with numerous

genetic regions found in the genome of a yellow fever vaccine virus (Centers for Disease

Control and Prevention). Dengvaxia was introduced in the Philippines, in April 2016. In

November 2017, more than 830,000 kids had at least one dose of the vaccine (Dengvaxia is

administered as three injections, with six-month intervals). In February 2018, the vaccination

had become a national scandal. Rumors says that Dengvaxia was linked to the deaths of

children. Many parents of children who died blamed the vaccine. Most of the deaths were

caused by internal bleeding in the heart, lungs, and brain, which are symptoms of

hemorrhagic dengue. (Wikipedia, 2021).

It still remains unclear why dengvaxia is still considered as an effective prevention

for dengue when in 2018, Sanofi Pasteur admitted that dengvaxia may increase the risk of

severe dengue in those who has no dengue infection before they were vaccinated

(Inquirer.net, 2019). Dengvaxia vaccine will only allow people who had been exposed to

dengue virus before to take it, but why make a prevention to dengue when it can only be

apply to specific people?

A considerable amount of research of the plant Oregano and African Talisay will

show us it’s efficacy on preventing the spread of dengue cases here in our country and

globally. Oregano is a plant that contains thymol, carvacrol, 1,8-cineole and p-cymene

substance (Science Direct, 2016), while African Talisay has tannins, saponins, Flavonoids,
and Terpenoids which is toxic for insects (Farrah, 2019). Different kinds of test will also be

conducted to show the efficacy of Oregano and African Talisay Leaves as mosquito repellent.

Test will also help us prove its safeness to people who already experienced dengue and to

people who did not experience the said disease before using it.

Hypothesis

HO: There is no significant effect on using Mosquito Repellent Spray made from Oregano

leaves and African Talisay leaves as a mosquito repellent.

H1: There is a significant effect on using Mosquito Repellent Spray made from

Oregano leaves and African Talisay leaves as a mosquito repellent.

Procedure

1. Making experimental box

2. Collection of mosquito wrigglers

3. Collect the plant materials

4. Making the Mosquito Repellent

a. Pour half a cup of distilled water with 10pcs.Oregano and 1/8kilo of African

Talisay leaves into a sauce pan, then boil, to extract the leaves’ sap.

b. Chop the remaining oregano and African Talisay leaves to be put on the spray

bottle later on.

c. If the water with oregano leaves and African Talisay leaves have boiled,

transfer the water into the spray bottles and add the chopped leaves into it.

d. Using the boiled leaves, extract the oil of the leaves and put it on the spray

bottles for the scent.

e. Mix thoroughly the extracted oil of the leaves with the boiled distilled water.

5. Spray the finish product and observe the mosquito after spraying the repellent
 6. Test the finish product to the laboratory before using it.

Materials

 1kl of nails

 45 pieces of 20x20 plywood

 (measurement of net)

 Mosquito wrigglers

 50 pieces of Oregano leaves

 1 kilogram of African Talisay leaves

Data List

1. Effectiveness of the alternative mosquito repellent.

2. Number of mosquitoes are injured, collapsed, dead.

3. Minuet it takes for the mosquitoes to reacts.

Risk Assessment

This pesticide study is supposed to kill mosquitoes, though a large amount of

pesticide on human skin may lead to irritation and dermatitis (Environ Med, 2001). Making

sure that this pesticide should only be use on mosquitoes and not for other animals is a must.

Upon conducting this study, we will strictly follow a proper and safe way to perform this

study. Proper observation is a must upon conducting this experiment.

Bibliography