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    Chapter 1 - Topic 1 - BIOT3427 - 2023

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    Vĩnh Nguyễn Văn
    This document outlines course topics on genetic technology. Chapter 1 focuses on nucleic acid extraction and quantitation. Topic 1.1 discusses extracting deoxyribonucleic acid (DNA) and deoxyribonucleic acid (RNA), introducing molecular facilities and equipment used. It explains that nucleases like Dnase require cofactors like divalent cations Ca2+ and Mg2+ to function, while ethylenediaminetetraacetic acid (EDTA) chelates these ions to inhibit or deactivate nucleases, preserving genomic integrity. Nucleic acids should be stored in Tris-EDTA buffer solution at cold temperatures in ethanol or isopropanol for preservation.

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    Chapter 1 - Topic 1 - BIOT3427 - 2023

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    Vĩnh Nguyễn Văn
    7 views16 pages
    This document outlines course topics on genetic technology. Chapter 1 focuses on nucleic acid extraction and quantitation. Topic 1.1 discusses extracting deoxyribonucleic acid (DNA) and deoxyribonucleic acid (RNA), introducing molecular facilities and equipment used. It explains that nucleases like Dnase require cofactors like divalent cations Ca2+ and Mg2+ to function, while ethylenediaminetetraacetic acid (EDTA) chelates these ions to inhibit or deactivate nucleases, preserving genomic integrity. Nucleic acids should be stored in Tris-EDTA buffer solution at cold temperatures in ethanol or isopropanol for preservation.

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    This document outlines course topics on genetic technology. Chapter 1 focuses on nucleic acid extraction and quantitation. Topic 1.1 discusses extracting deoxyribonucleic acid (DNA) and deoxyribonucleic acid (RNA), introducing molecular facilities and equipment used. It explains that nucleases like Dnase require cofactors like divalent cations Ca2+ and Mg2+ to function, while ethylenediaminetetraacetic acid (EDTA) chelates these ions to inhibit or deactivate nucleases, preserving genomic integrity. Nucleic acids should be stored in Tris-EDTA buffer solution at cold temperatures in ethanol or isopropanol for preservation.

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    © All Rights Reserved
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    7 views16 pages

    Chapter 1 - Topic 1 - BIOT3427 - 2023

    Uploaded by

    Vĩnh Nguyễn Văn
    This document outlines course topics on genetic technology. Chapter 1 focuses on nucleic acid extraction and quantitation. Topic 1.1 discusses extracting deoxyribonucleic acid (DNA) and deoxyribonucleic acid (RNA), introducing molecular facilities and equipment used. It explains that nucleases like Dnase require cofactors like divalent cations Ca2+ and Mg2+ to function, while ethylenediaminetetraacetic acid (EDTA) chelates these ions to inhibit or deactivate nucleases, preserving genomic integrity. Nucleic acids should be stored in Tris-EDTA buffer solution at cold temperatures in ethanol or isopropanol for preservation.

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    of 16

    GENETIC TECHNOLOGY

    Le Huyen Ai Thuy, PhD, Professor


    Lao Duc Thuan, PhD
    Truong Kim Phuong, PhD
    Corresponding: phuong.tk@ou.edu.vn
    Course Topics (Theoretic lessons)
    Chapter 1. Nucleic acids extraction & Quantitation of nucleic acids
    Chapter 2. Working with enzymes
    Chapter 3. Molecular hybridization
    Chapter 4. Vectors and Gene cloning
    Chapter 5. Polymerase Chain Reaction method
    Chapter 6. Nucleic acid sequencing.
    Chapter 1. Nucleic acids extraction
    Quantitation of nucleic acids

    Topic 1.1. Nucleic acids extraction

    Topic 1.2. Quantitation of nucleic acids


    Topic 1.1. Nucleic acids extraction

    ❖ Topic contents

    1.1. Dideoxyribonucleic acid extraction.


    1.2. Deoxyribonucleic acid extraction.
    1.3. Introduction: molecular facilities and equipment.
    Nuclease: Dnase => thủy giải DNA
    Dnase: cần “cofactor” là các ion hóa trị
    2: Ca2+; Mg2+
    EDTA: hấp phụ các ion hóa trị 2: Ca2+;
    Mg2+
    Hệ quả: EDTA ức chế/bất hoạt Dnase
    => đảm bảo tính toàn vẹn của bộ gen,
    DNA cần tách chiết
    Bảo quản trong dung dịch TE: Ethanol tuyệt đối trữ lạnh.
    - Tris-HCl (pH 8) Isopropanol
    - EDTA
    - The end of Topic 1.1 -

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