King Salman International University

Branch: Ras Sudr

Faculty: Pharmacy
Program: Bachelor of Pharmacy-PharmD (Clinical Pharmacy)
Department: ……………………………….

Assignment #2
SPRING SEMESTER (2222)
ACADEMIC YEAR (2022- 2023)

Course Name Pharmaceutical Course Code PMB202 Date

Microbiology
No. of pages …. Total Marks 10 Time Allowed …….

Student Name Student ID Level …..

Please Answer All Questions

Choose the correct answer for each of the following Each …. Mark, …… Min

1. By the end of the sterilizing period, the autoclave is opened:

(a) while air and steam discharge valve is closed (b) when pressure gauge falls to zero
(c) when steam is seen to emit from the safety valve (d) when temperature falls to zero
2. All the following are correct regarding saturated steam, EXCEPT:
(a) sterilizes objects in shorter time (b) behaves like hot air (condensate less readily)
(c) has high penetration power (d) gives uniform temperature
3. All the following objects can be sterilized by ethylene oxide, EXCEPT:
(a) preparations in sealed glass or metal containers (b) plastic syringes
(c) reusable medical and surgical instruments (d) surgical dressings and sutures
4. The best method for sterilizing glass petri dishes is:
(a) dry heat (b) gamma-rays (c) formaldehyde (d) moist heat

5. Incinerators are:
(a) less effective method of killing harmful microorganisms
(b) used to destroy contaminated waste materials from hospital
(c) used for sterilization of petri dishes (d) examples of moist heat sterilizers

6. Exponential killing kinetics are represented by:

(a) a linear portion on survivor curves (b) an irregular tailing on survivor curves
(c) a peak on survivor curves (d) all of these

7. Thermal death point is defined as the lowest temperature required to kill a microbial population
in 10 min
(a) True (b) False
8. De-pyrogenation of glassware can be achieved by:
(a) washing (b) disinfection (c) boiling (d) gamma radiation (e) any of these

Course Code PMB202 Page 1 of 3

 King Salman International University
Branch: Ras Sudr
Faculty: Pharmacy
Program: Bachelor of Pharmacy-PharmD (Clinical Pharmacy)
Department: ……………………………….
9. Bacterial spores are a problem in sterilizing instruments and equipment because:
(a) they are easy to kill, but are usually protected by organic matter
(b) they are resistant to physical and chemical agents (c) they are resistant to antibiotics
(d) most pathogenic bacteria are spore-formers (e) all of these

10. In autoclave, applying pressure:

(a) denature cellular proteins (b) disrupt cell membrane
(c) increase microbial sensitivity to heat (d) non of the above

11. Latent heat is released more readily in case of:

(a) dry saturated steam (b) boiling water (c) superheated steam
(d) both a & c (e) all of the above

12. Ultra violet (UV) radiation is antimicrobial because the energy present causes links between
adjacent thymines in the DNA molecules, which can cause accumulation of lethal mutations.
(a) True (b) False

13. Sterilization can be achieved by:

(a) boiling for 10 min (b) boiling for 60 min
(c) steaming at 121oC/5 min (d) free flowing steam/10 min (e) non of these

14. The presence of air in steam should be avoided because the former:
(a) causes superheating (b) hinders steam penetration
(c) prevents load drying (d) increases steam temperature

15. Calcium and dipicolinic acid make bacterial spores resist thermal treatment by preventing:
(a) moisture penetration (b) heat penetration (c) both a&b (d) none of these

16. For which of the following sterilants Z value can be determined:

(a) ethylene oxide (b) gamma radiation (c) IR radiation (d) none of these

17. HEPA filters

(a) function more like sieves (b) are used filtration of chemical reagents
(c) can remove both dead and viable bacteria (d) none of these

18. In sterility testing, aerobic bacterial contaminants can be recovered using:

(a) fluid thioglycolate medium/35oC/48 h (b) Sabouraud’s dextrose broth/35oC/14 days
(c) Sabouraud’s dextrose broth/25 C/48 h
o
(d) fluid thioglycolate medium/35oC/14 days

19. Air quality of class 100

(a) is required for manufacturing oral antibiotic suspensions
(b) is required for terminally sterilized injectable ampoules
(c) contains 100 viable particles per one cubic foot (d) non of these

20. Rapidity of sterilization by heat depends on:

Course Code PMB202 Page 2 of 3

 King Salman International University
Branch: Ras Sudr
Faculty: Pharmacy
Program: Bachelor of Pharmacy-PharmD (Clinical Pharmacy)
Department: ……………………………….
(a) number of microorganisms (b) exposure temperature
(c) contained medium of microorganisms (d) only a & b (e) all of these

END OF Quiz QUESTIONS

Good Luck
Examiners
Course Coordinator

Course Code PMB202 Page 3 of 3