GROVE CITY COLLEGE DEPARTMENT OF EDUCATION

Andrew Musacchio/ AP Biology/ 10-20-2022

 Topic
o Preparing potatoes for Osmosis and Diffusion Lab
o Potato Core Example Problem
o Water Potential Problems Worksheet
 Objectives
o ENE-2.H, After instruction TSWBAT explain how concentration gradients affect
the movement of molecules across membranes with 80% accuracy on an end of
unit exam.
o ENE-2.I, After instruction TSWBAT explain how osmoregulatory mechanisms
contribute to the health and survival of organisms with 80% accuracy on an end of
unit exam.
 Teaching Procedures
o Anticipatory Set (3 min)
 Ask the students to explain the video from yesterday
 The U-shaped tube video where water flowed towards the higher
solute concentration side
 Have the students explain to you how osmosis deals with free
water, and when there is a solute present free water turns into
bound water which therefore cannot act on the system
 This then results in water flowing from the low-concentration
solute side to the high-concentration solute side as it did in the
video
o Independent Practice 1 (10 min)
 Have the students choose 6 potato cubes and get 6 cups
 Have the students label one cup of distilled water, the next 0.2M, the next
0.4M, the next 0.6M, the next, 0.8M, and finally the last cup of 1.0M
sucrose
 Have students record the weight of each potato they are going to place in
each cup
 Have the students pour the respective solution into the appropriate cup just
so the potato cube is fully covered
 Have students cover the top of the cup with parafilm to prevent the
evaporation of water
 Have students put all cups in the back of the classroom so they will not be
disturbed over the next day
o Development 1 (8 min)
 Use the potato core example presentation to explain using a graph to find
out the water potential of a potato cell
 Go through each slide and have the students take notes
  Tell them this is what they are trying to make for the Osmosis and
Diffusion lab
o Independent Practice 2 (20 min)
 Pass out the Water Potential Problems worksheet
 Tell the students they can work quietly with the person next to them
 Walk around as the students work to make sure they are on task and
answer any questions they may have
o Closure (3 min)
 Ask the students what would happen to the water potential of a potato cell
if we left it out to dry
 It would decrease because as more water left the cell the
concentration of the solute will increase therefore being less free
water and therefore less water potential
 Materials
o 100 mL of Distilled water
o 100 mL of 0.2 M Sucrose
o 100 mL of 0.4 M Sucrose
o 100 mL of 0.6 M Sucrose
o 100 mL of 0.8 M Sucrose
o 100 mL of 1.0 M Sucrose
o 30 small cups
o 30 potato cores
o Scale
o Potato Core Example Presentation
o Water Potential Problems Worksheet
 Adaptations
o None
 Evaluation
o Formative
 Water Potential Practice Problems
o Summative
 There will be an end of unit exam where students will demonstrate their
knowledge of osmosis, diffusion, and how osmoregulatory mechanisms
help maintain homeostasis in organisms
 Reflection
o Did the students understand the potato core example? Was there any confusion on
a specific part? How can these misunderstandings be corrected? Is there a video
that can be posted so students can refer to it to help their understanding?