Ultimate 3000 2g Pumps Service Instructions v1-6

Thermo Scientific
UltiMate 3000 Series

SD, RS, BM, and BX Pumps
(Second-Generation Pumps)
Service Instructions
Revision: 1.6
Date: January 2017
© 2017 Thermo Fisher Scientific

Doc. No. 4820.4060
- Thermo Fisher Scientific Confidential -
UltiMate 3000 Series:
Table of Contents
1 Introduction ................................................................................................................... 1
1.1 How to Use these Service Instructions ...................................................................... 1
1.2 Additional Documents ............................................................................................... 3
1.3 Instructions for Returning Instruments or Parts ......................................................... 4
2 Preparatory Measures .................................................................................................. 5

2.1 General Care and Precautions .................................................................................... 5
2.2 Safety Precautions ...................................................................................................... 6
2.3 Precautions for Connecting and Operating an ISO-3100BM .................................... 7
3 General Pump Design ................................................................................................... 8

3.1 Main Instrument Modifications ................................................................................. 8
3.2 Rear Panel ................................................................................................................ 10
3.3 Power Connection .................................................................................................... 11
3.4 Fuse Cartridge (Main Power Fuses) ........................................................................ 11
3.5 Interior Components and Liquid Flow Path............................................................. 11
3.6 View into the Enclosure ........................................................................................... 12
4 Instrument Checkout .................................................................................................. 14

4.1 Main Board .............................................................................................................. 14
4.2 Pump Block Boards ................................................................................................. 16
4.3 Switching Power Supply .......................................................................................... 17
5 Chromeleon Service Functions .................................................................................. 18

5.1 Pump Diagnostics .................................................................................................... 18
5.2 Predictive Performance and Service Interval ........................................................... 19
5.3 Service Commands and Properties .......................................................................... 20
5.3.1 Changing the Display Filter Level ................................................................... 20
5.3.2 Service Level Commands and Properties ........................................................ 22
5.3.3 Setting the Pump into the Service Mode.......................................................... 25
5.4 Service Panel (Chromeleon 6.80 only) .................................................................... 26
6 Maintenance and Service ........................................................................................... 28

6.1 General Information and Precautions ...................................................................... 28
6.2 Connecting Capillaries ............................................................................................. 30
6.3 Recommended Tools ............................................................................................... 33
6.4 Required Parameter Updates or Adjustments .......................................................... 35
6.4.1 Calibrating the Pressure Transducer for the System Pressure ......................... 37
6.4.2 Setting the Offset for the Pressure Transducer (System Pressure) .................. 38
6.4.3 Adapting the Brightness and Contrast of the Front Panel Display .................. 38
6.5 Removing and Installing the Side Panels................................................................. 40
Service Instructions Thermo Fisher Scientific Confidential Page i

6.6 Top Cover ................................................................................................................ 42

6.6.1 Removing and Reinstalling the (Existing) Top Cover ..................................... 42
6.6.2 Replacing the Top Cover ................................................................................. 43
6.7 Replacing the Main Power Fuses ............................................................................. 44
6.8 Purge Unit ................................................................................................................ 45
6.8.1 Replacing the Purge Unit ................................................................................. 45
6.8.2 Replacing the Purge Valve Knob ..................................................................... 47
6.8.3 Replacing the Cap Seal .................................................................................... 48
6.9 Replacing the Check Valve Cartridges .................................................................... 49
6.10 Pump Head, Pistons, and Piston Seals ................................................................. 51
6.10.1 Visually Inspecting the Pump for Piston Seal Leakage ............................... 57
6.10.2 Pump Head and Pistons ............................................................................... 60
6.10.3 Replacing the Piston Seals ........................................................................... 65
6.11 Rear Seal Wash System ....................................................................................... 77
6.11.1 Inspecting the Rear Seal Wash System for Leakage ................................... 77
6.11.2 Replacing the Detector ................................................................................. 77
6.11.3 Cleaning the Detector Electrodes................................................................. 78
6.11.4 Replacing the Peristaltic Tubing .................................................................. 79
6.11.5 Replacing the Peristaltic Pump .................................................................... 80
6.12 Leak Sensor .......................................................................................................... 82
6.12.1 Drying the Leak Sensor (Eliminating Leakage) .......................................... 82
6.12.2 Replacing the Leak Sensor........................................................................... 83
6.12.3 Adjusting the Leak Sensor Sensitivity ......................................................... 84
6.13 Mixing System ..................................................................................................... 85
6.13.1 Replacing the Capillary Mixer and/or Static Mixer..................................... 85
6.13.2 Checking the Static Mixer for Permeability ................................................ 87
6.13.3 Replacing the Filter Frit in the Previous Static Mixers ............................... 88
6.14 Inline Filter........................................................................................................... 92
6.14.1 Inline Filter (ISO-3100) ............................................................................... 92
6.14.2 Inline Filter (LPG-3400BM, DGP-3600BM) .............................................. 94
6.14.3 Checking the Inline Filter for Permeability ................................................. 96
6.15 Replacing the Solvent Selector or Proportioning Valve ...................................... 97
6.16 Replacing the Main Board ................................................................................. 100
6.17 Replacing a Pump Block .................................................................................... 103
6.18 Replacing the Switching Power Supply............................................................. 108
6.19 Testing the Pump for Leakage ........................................................................... 110
6.20 Front Panel Door ................................................................................................ 113
6.20.1 Replacing the Front Panel Display ............................................................ 113
6.20.2 Replacing the Front Panel LED Board ...................................................... 115
6.21 Vacuum Degasser (LPG-3400) .......................................................................... 116
6.21.1 Rinsing the Degassing Channels ................................................................ 116
6.21.2 Replacing a Degassing Chamber ............................................................... 117
6.21.3 Degasser Vacuum Pump ............................................................................ 118
6.22 Pulse Damper ..................................................................................................... 121
6.22.1 Replacing the Pulse Damper ...................................................................... 121
6.22.2 Replacing the Pulse Damper Membrane.................................................... 122
6.23 Shutting Down the Pump ................................................................................... 123
Service Instructions Thermo Fisher Scientific Confidential Page ii

7 Troubleshooting ........................................................................................................ 124

7.1 Overview ................................................................................................................ 124
7.2 Pump Block Status LED ........................................................................................ 125
7.3 Messages on the Pump Display ............................................................................. 126
7.4 Chromeleon Diagnostics Messages ....................................................................... 137
7.5 Operating Problems ............................................................................................... 140
7.6 Checking the Compression Values ........................................................................ 148
7.7 Checking the Pressure Transducers ....................................................................... 149
7.8 Recording the Service Channels/Contacting Support ............................................ 151
8 Updating the Pump Firmware ................................................................................. 154
9 Interior Components and Flow Path ...................................................................... 156

9.1 ISO-3100 ................................................................................................................ 157
9.1.1 ISO-3100SD ................................................................................................... 157
9.1.2 ISO-3100BM.................................................................................................. 159
9.2 LPG-3400 ............................................................................................................... 161
9.2.1 Interior Components ...................................................................................... 161
9.2.2 Flow Path ....................................................................................................... 162
9.3 DGP-3600 .............................................................................................................. 164
9.3.2 Flow Path ....................................................................................................... 165
9.4 HPG-3200 .............................................................................................................. 167
9.4.2 Flow Path ....................................................................................................... 168
9.5 HPG-3400 .............................................................................................................. 170
9.5.2 Flow Path ....................................................................................................... 171
10 Optimizing the Pump for Specific Applications..................................................... 173
11 Connecting a Corona or Colouchem III Detector.................................................. 177
12 Consumables and Spare Parts ................................................................................. 178
13 Appendix .................................................................................................................... 191

13.1 Digital I/O (Pin Assignment) ............................................................................. 191
13.2 Solvent Rack (Pin Assignment) ......................................................................... 192
Addendum :
Troubleshooting— Recommendations for Leakage-Free Pump Operation
Service Instructions Thermo Fisher Scientific Confidential Page iii

1 Introduction
1.1 How to Use these Service Instructions
The service manual is intended only for authorized Thermo Fisher Scientific service
personnel or service personnel specifically authorized by Thermo Fisher Scientific. It is
intended to assist in the maintenance and repair of the second-generation pumps in the
Thermo Scientific™ UltiMate 3000 series, which are listed further down in this section.
Do not make the manual available to other individuals.
The individual using this manual must have sufficient training in the service of analytical
instrumentation. The individual must be aware of the potential hazards including (but not
limited to) electrical hazards, chemical solvent hazards, and the exposure to pressurized
solvents.
The service manual is intended as a supplementary guide to the Operating Instructions for
qualified service engineers. For the latest information, also see the Product Support
Bulletins (= PSBs) for the autosamplers. Thermo Fisher Scientific cannot be held liable for
any repairs or maintenance work carried out by unqualified personnel.
This manual enables you to solve problems effectively that may occur with the following
pumps in the UltiMate™ 3000 series:
• SD pumps (ISO-3100SD, LPG-3400SD, DGP-3600SD, and HPG-3x00SD)
• SDN pumps (LPG-3400SDN and DGP-3600SDN)
• RS pumps (LPG-3400RS, DGP-3600RS, and HPG-3x00RS)
• BM pumps (ISO-3100BM, LPG-3400BM, and DGP-3600BM)
• HPG-3200BX
Before performing any repair or maintenance procedures, read both the Operating
Instructions and these Service Instructions thoroughly to obtain a general idea of the design of
the instrument.
The following conventions apply to the descriptions throughout this manual:
• Almost all descriptions in this manual apply to all pumps listed above. Therefore, the
term "the pump" is used throughout the manual. If some detail applies to only one pump
model or version, the model (version) is identified by name.
For first-generation pumps, such as A, AB, M, MB, and P pumps (for example,
LPG-3400A, DGP-3600MB, or HPG-3200P), a separate service manual is available (see
UltiMate 3000 Pump Series Service Instructions).
• If not otherwise stated, the descriptions for Viper™ capillary connections apply also to
nanoViper™ and other Viper capillary connections. Therefore, the term "Viper" is used.
• The module configuration may vary. Therefore, not all descriptions necessarily apply to
your particular instrument.
Service Instructions Thermo Fisher Scientific Confidential Page 1

• The representation of a component in this manual may be different from the real
component. However, this does not influence the descriptions.
• The software descriptions in this manual refer to Chromeleon™ 6.80. If the pump is
operated from Chromeleon 7, refer to section 5 for information about the main
differences (→ page 18).
At various points throughout the manual, the following symbols indicate messages of
particular importance:
Tip: Indicates general information intended to optimize the service and

maintenance steps or the performance of the instrument.
Important: Indicates that failure to take note of the accompanying information may
result in damage to the instrument.
Warning: Indicates that failure to take note of the accompanying information may
result in personal injury.
This manual is provided "as is". Every effort has been made to supply complete and
accurate information and all technical specifications have been developed with the utmost
care. The information contained in this manual should not be construed as a commitment
by Thermo Fisher Scientific. Thermo Fisher Scientific assumes no responsibility for any
errors that may appear in this document that is believed to be complete and accurate at the
time of publication and, in no event, shall Thermo Fisher Scientific be liable for incidental
or consequential damages in connection with or arising from the use of this document. We
appreciate your help in eliminating any errors that may appear in this document.
The information contained in this document is subject to change without notice.
All rights reserved, including those for photomechanical reproduction and storage on
electronic media. No part of this publication may be copied or distributed, transmitted,
transcribed, stored in a retrieval system, or transmitted into any human or computer language,
in any form or by any means, electronic, mechanical, magnetic, manual or otherwise, or
disclosed to third parties without the express written permission of Thermo Fisher Scientific
Inc.
Trademarks
Luer-Lok is a trademark of BD Becton, Dickinson and Company.
MP35N is a trademark of SPS Technologies.
PharMed is a trademark of Saint -Gobain Performance Plastics.
Torx is a trademark of TEXTRON INDUSTRIES, Inc.
Microsoft and Windows are trademarks of Microsoft Corp.
All other trademarks are property of Thermo Fisher Scientific and its subsidiaries.

1.2 Additional Documents

In addition to the service instructions, the following documents are available:
• Operating Instructions
• Service Return Form (including Decontamination Certificate)
• Warranty Return Form
• OQ PQ Instructions
• Product Support Bulletins
• Maintenance instructions for UltiMate 3000 Modules
• Packing Instructions (for shipping the instrument)

1.3 Instructions for Returning Instruments or Parts

If you need to return the instrument (or part) for depot repair or for exchange under
warranty, observe the following:
1. Contact your local Thermo Fisher Scientific support center or the support center
authorized by Thermo Fisher Scientific for your region.
The support center will let you know the procedure that you have to follow and the
information and documentation that you have to provide with the instrument (or part).
Warning: Possible Contamination

Hazardous substances may have contaminated the instrument (or part)
during operation and may cause personal injury to service personnel.
• Decontaminate all parts of the instrument (or part) that you want to
return.
• Fill in and sign the Decontamination Certificate to confirm that the
instrument (or part) has been properly decontaminated and that it is
free of hazardous substances.
• Missing or incompletely filled in Decontamination Certificates will
delay processing and may result in the instrument (or part) being
returned without being inspected or repaired, or without credit being
issued.
2. Shut down and prepare the instrument for transport, observing the instructions
on page 123.
3. Carefully pack the instrument (or part), observing the packing instructions for the
instrument.

2 Preparatory Measures
2.1 General Care and Precautions
The surfaces of the pump are resistant to weak acids, alkali, and organic solvents.
Nevertheless, immediately wipe up all liquids spilled onto the pump surface, using lint-free
cloth or tissue (avoid rubbing). If surfaces are exposed for longer periods, these liquids can
cause damage.
Observe the following general precautions:

• Maintenance and service procedures must be carried out only by qualified personnel. All
service personnel must observe the general care and precautions and all safety
information, specific safety messages, and precautionary statements presented in these
service instructions.
In addition, all service personnel must be familiar with and observe all safety precautions
presented in the most recent revision of the Operating Instructions for the pump.
• The fluid components of the pump may be filled with toxic solvents. Before starting
maintenance or repair work, rinse the fluidics with a suitable solvent. Put on appropriate
protective clothing and wear goggles.
• Some capillaries of the RS and ISO-3100BM pumps and some Viper system capillaries
are made of MP35N™, a nickel-cobalt based alloy. Individuals with sensitivity to
nickel/cobalt may show an allergic reaction from skin contact. Put on protective clothing
as appropriate.
• Use only the replacement parts and additional components, options, and peripherals
specifically authorized and qualified for the pump by Thermo Fisher Scientific. Thermo
Fisher Scientific cannot be held liable for any damage, material or otherwise, resulting
from the use of non-authorized components or parts.
• When operating the pump with the enclosure open, take special caution because of the
line voltage. Observe the warning notes in these service instructions.
• Do not forget to test the pump for leakage after you have carried out maintenance or
repair work on the fluid connections (→ page 110).
• When turning on the pump for test purposes, observe the installation instructions in the
Operating Instructions.
• Before you start any maintenance or service procedure, see page 28 for specific
information to be considered.

2.2 Safety Precautions

When working with analytical instrumentation, you must know the potential hazards of
using chemical solvents. Be familiar with and observe all safety precautions presented in
the most recent revision of the Operating Instructions for the pump.
Before you carry out any maintenance or repair procedures inside the pump enclosure, take
the following measures to prevent operator injury or damage to the pump:
1. Rinse toxic solvents from the pump if applicable. Avoid inhaling any vapors. Always
wear gloves and goggles.
2. Set the pump flow to 0 and wait until the system pressure is almost down (< 1 MPa).
3. If the pump is connected in Chromeleon, select the Disconnect command in
Chromeleon to terminate communication with the data system.
4. Turn off the pump and disconnect the power cord from its source.
5. ISO-3100 and HPG-3200
Keep the following in mind when performing pump maintenance:
Hydrostatic pressure builds up in the system when the fluid connections of the pump are
filled with liquid and the solvent reservoir are located above the pump outlet during
pump operation. Thus, eluent may escape when you open a fluid connection in the pump.
Therefore, before you open a fluid connection, position the reservoirs below the
connection to be opened.
Warnings: Do not operate the pump when the enclosure is open. The drive belts on
the pump blocks are running (risk of injury).
Be aware that some components inside the enclosure may carry line
voltage even after the power cord has been disconnected from its
source.Wait 10 seconds (minimum) before disconnecting or connecting
cables in the module.
Sharp edges may be present inside the device and can cause minor
injury. To prevent injury, be careful when getting in contact with sharp-
edged parts.
Avoid looking directly into the pump light LED. Do not use light
focusing instruments for viewing the light beam. The high luminosity of
the lamp can be harmful to the eyes.
Important: To avoid electrostatic discharge, which may result in damage to

electronic components, the persons performing the service must use
appropriate grounding protection.

2.3 Precautions for Connecting and Operating an ISO-3100BM

Note the following if you want to connect a Corona™ or Coulochem™ detector to the
pump (for more details, see the Chromeleon Help):
• To connect the detector to a digital I/O port on the rear panel of the pump, use the 6-pin
mini-DIN signal cable (part no. 6000.1004).
• To connect the detector to the pump, follow the instructions in the Chromeleon Help. The
Help provides separate sections for connecting these detectors to an UltiMate 3000
pump, including details about the appropriate cabling and settings required in the Server
Configuration program.
• Only Corona
If an error occurs in the detector, pumping is stopped and a message appears on the pump
display and in the Chromeleon Audit Trail ('An emergency stop was requested over the
digital input line') only if the pump and detector are connected and configured as
described in the Chromeleon Help. Check the detector and take appropriate remedial
action (→ Detector manual).
• Only Coulochem III
When the pump and detector are connected and configured as described in the
Chromeleon Help, the detector cell is automatically turned off when the pump flow is
zero.
When operating an ISO-3100BM, observe the following:

• Hydrostatic pressure builds up in the system when the fluid connections of the pump are
• Avoid sudden pressure drops at the pulse damper, for example, by opening the purge
valve. Damage to the pulse damper may result.
• To prevent eluent from suddenly escaping any connections, open capillary connections in
the pump and on to the column only when the system pressure is almost down. Wait until
the pressure is less than 1 MPa before opening a connection.
• If you can reduce the pressure only by opening the purge valve, turn the valve knob
slowly counterclockwise to allow sufficient time (minimum 5 seconds) for the pressure to
bleed to zero.
• When using the pump with an electrochemical (EC) Coulochem III detector:
♦ Configure the system to automatically turn off the EC cells when a pump or system
error occurs (→ Coulochem III User Manual, section 2.7.7 Inputs).
♦ Always turn off the EC cells first before shutting down the pump or performing
maintenance tasks.

3 General Pump Design

3.1 Main Instrument Modifications
The serial numbers in the table refer to the first module shipped with the described
modifications.
Serial Number Modifications Remarks
8086058 ISO-3100BM pump is suitable for a To operate the pump up to the maximum
maximum working pressure of working pressure, pump firmware version
62 MPa as a standard (≥ 3.43) and Chromeleon 6.80SR10 or
7.2SR1 or later are required.
All capillaries used with the ISO-3100BM
pump are now MP35N Viper capillaries.
8063776 RS pumps are fully biocompatible Internal pump capillaries are MP35N
Viper capillaries.
8062411 Pump heads of RS, SD, and BM Pump heads have NP seals installed in the
pumps plate of the rear seal wash system
8034523 Main board revision B Extended Digital I/O functionality

(→ page 191)
LPG-3400 pumps:
The connector for the degasser data cable
is no longer available on the main board
(→ page 15).
8030113 HPG-3x00RS pumps are shipped For information about the mixing systems
with the 200 µL mixing system as a that are available for SD and RS pumps,
standard (instead of the 400 µL see section 10 (→ page 173).
mixing system.)
8030113 RS pumps are suitable for a To operate the pump up to the maximum
maximum working pressure of working pressure, Chromeleon 6.80 SR10
103 MPa as a standard. or later and an appropriate firmware
version (≥ 3.00) are required.
8027648 SD pumps are suitable for a To operate the pump up to the maximum
maximum working pressure of working pressure, Chromeleon 6.80 SR10
62 MPa as a standard. or later and an appropriate firmware
version (≥ 2.40) are required.
SD pumps with a maximum pressure
specification of 50 MPa can be upgraded
to 62 MPa. For part number information,
see the next table.
Note: All pumps shipped from July 2012 on have ceramic check valve cartridges installed as a standard.
103 MPa RS pumps have always been shipped with ceramics check valve cartridges as a standard.

The following upgrade kits (including detailed installation instructions) are available:
Upgrade Kit Part No.
To upgrade SD pumps to 62 MPa

ISO-3100SD and LPG-3400SD 6040.0011
HPG-3x00SD and DGP-3600SD 6040.0021

3.2 Rear Panel
1 2 3 4 5 6 7
Fig. 1: Rear panel view
No. Description
1 Power switch
2 Fuse cartridge
3 Main power receptacle
4 Digital I/O ports for communication with external devices, by using the 6-pin Mini-DIN
signal cable (part no. 6000.1004). For pin out and cable details, see page 191.
5 Solvent Rack port for connection of a SRD-3x00 Solvent Rack
For pin out details, see page 192.
6 USB hub (3 USB ports, USB 2.0 or 1.1)
Depending on the UltiMate 3000 system configuration, for connection of one UltiMate 3000
system module each or for connection of one USB hub each.
7 USB port (USB 2.0 or 1.1) for connecting the pump to the Chromeleon computer.
Note: If you want to connect a Corona or Coulochem III detector to the pump, refer to
the pump manual and/or the Chromeleon Help for details about the correct
cabling, pin assignment, and settings required in the Server Configuration
program.

3.3 Power Connection

Use the power cord shipped with the pump to connect the instrument to the main power
source. Connect the power cord from the main power receptacle on the rear panel to a
power source that is connected to a true ground. You need not adapt the line voltage to
local voltage requirements.
For minimum interference effects, all components of the UltiMate 3000 system and the
Chromeleon computer should be connected to the same mains output (same phase)
Use the main power switch on the rear panel to turn the pump on and off.
3.4 Fuse Cartridge (Main Power Fuses)

The pump uses two slow-blow fuses rated at 2 A, 250 V. They are installed in a fuse
cartridge that is part of the main power receptacle on the rear panel.
For replacement instructions, see page 44. Replacement fuses must be the same type and
rating as specified in this manual.
3.5 Interior Components and Liquid Flow Path

The interior components and liquid flow path depend on the pump.
For the … Find the … On page
ISO-3100SD Interior components 157

Liquid flow path 158
ISO-3100BM Interior components 159
LPG-3400 Interior components 161
DGP-3600 Interior components 164
HPG-3200 Interior components 167

3.6 View into the Enclosure

The photo provides an overview of how the components of a pump are arranged in the
enclosure (here HPG-3400RS). However, the arrangement is similar for all pump types.
2
14
5a
5b
13
6
12
7
11
10
8 8
Fig. 2: Interior view—view from above into the enclosure (here HPG-3400RS)
No. Description
1 Rear panel
2 Main power receptacle
3 Switching power supply
4 Depending on the pump type and/or pressure specification of the pump
A fan is installed in this position (→ page 13)
5a Pump block (right, HPG and DGP only)
5b Pump block (left)
6 HPG only: Pressure transducer cable from right pump block (working pressure)
7 HPG and DGP (right pump) only:
Pressure transducer cable from purge unit (system pressure)
8 Pump lights
9 Interior front panel

No. Description
10 Cable from detector of seal wash system
11 HPG only:
Pressure transducer cable from left pump block (working pressure)
12 ISO, LPG, and DGP (left pump) only:
Connector for pressure transducer cable from purge unit (system pressure)
13 Motor of peristaltic pump
14 Main board
It depends on the pump type and/or pressure specification of the pump whether a fan is
installed in the position indicated in the picture.
Fig. 3: Interior view—here fan installed in an HPG-3400RS

4 Instrument Checkout
Warning: When working on the electronics, verify that the power cord is
disconnected from its source. Use appropriate earthing protection to avoid
electrostatic discharge, which may result in damage to electronic
components.
4.1 Main Board

To access the main board follow the steps in section 6.16 (→ page 100).
1 2 3 4
8d
11
10
9 8c 8b 8a 7 6 5
Fig. 4: Main board (here revision A)
No. Function Remark
1 USB port (USB 2.0 or 1.1) Connection between the pump and the Chromeleon
computer
2 USB hub Depending on the UltiMate 3000 system configuration,
(3 USB ports, USB 2.0 or 1.1) for connection of either one UltiMate 3000 system
module or one USB hub each.
3 Solvent Rack port Connection of a SRD-3x00 Solvent Rack
4 Connectors for digital I/O See section 13.1 (→ page 191)
ports 1 and 2
5 Connector for the power supply The low voltage cable from the switching power supply
connects here.
6 Connector for the pump block cable Pumps with two pump blocks only:
The control cable from the right pump block is
connected here.
7 Connector for the fan cable Depending on the pump type and/or pressure
specification of the pump
The control cable for the fan is connected here.

No. Function Remark
8 Connectors for the degasser vacuum With LPG-3400 pumps only

pump - with: Depending on the degasser vacuum pump
8d Ribbon cable for degasser Connected with the degasser vacuum pump with which
or status and power supply the pump is shipped from the factory
8a + Status cable Connected with replacement degasser vacuum pumps

8b power supply cable Note: The connector for the data cable is no longer
(+ 8c) data cable available on main board revision B. With this revision,
connecting the data cable is no longer required.
9 Connector for the pump block cable The control cable from the pump block is connected
here.
Pumps with two pump blocks:
This is the cable from the left pump block.
10 Connector for the standby button
11 Connector for the display cable

4.2 Pump Block Boards

To access the pump block boards follow the steps in section 6.17 (→ page 103).
The pump type determines whether the pump has one or two pump blocks and which
components are connected. However, the arrangement of the components on the boards is the
same for all pump types. The picture shows the pump blocks in a two-block pump.
11 12 13 1 2 11 12 13 1 2
9 8 7 6 5 4b 10 3 9 6 5 4a
Fig. 5: Pump block boards (here in an HPG-3400RS)
(here: board version without revision letter on the board)
No. Description
1 Pump block cable

2 Connector for the hall sensor
3 Connector for the leak sensor
4a HPG-3x00: Connector for the system pressure transducer
DGP-3600: Connector for the system pressure transducer of the right pump
4b LPG-3400 and ISO-3100: Connector for the system pressure transducer
DGP-3600: Connector for the system pressure transducer of the left pump
5 Connector for the working pressure transducer
6 Pump light board
Note: The pump light board on the right is available also in LPG-3400 pumps. The supply cable
from the board is connected to connector no. 10.
7 Connector for the detector of the rear seal wash system
8 Connector for the peristaltic pump
9 Depending on the pump type:
HPG-3400: Connector for the Solvent Selector
LPG-3400 and DGP-3600: Connector for the proportioning valve
10 LPG-3400 only: Connector for the cable from the pump light board on the right
11 Connector for the motor encoder
12 Connector for the motor hall sensor
13 Connector for the motor power

4.3 Switching Power Supply

To access the switching power supply follow the steps in section 6.18 (→ page 108).
Fig. 6: Switching power supply (24 V/150 W)
Name Function Remark
+V Terminals for 24 V, 6.3 A output. Power supply of the main board

Used to connect the red cable from the power
supply connector on the main board.
-V Terminals for 0 V (ground) output Power supply of the main board
Used to connect the black cable from the power
supply connector on the main board.
Earth terminals (ground) The ground cables must be connected to avoid
personal injury.
N Input terminal for line voltage Used to connect the blue cable
L Input terminal for line voltage Used to connect the black cable
Warning: Do not confuse the line voltage cables with the low-voltage cables!
+V ADJ Potentiometer for adjusting the supply The output voltage is set at the factory. The
voltage of the main board setting should not be changed.
Important: Whenever you have performed maintenance work on the switching

power supply, make sure that all cables are secured properly in the
correct terminals on the switching power supply. The line voltage
cables are secured by a cable tie. Verify that they are secured this way.
This is important for the operational safety of the pump.

5 Chromeleon Service Functions

The software details and terminology in this manual refer to Chromeleon 6.80.
If the pump is operated from Chromeleon 7, note the following main differences:
• Chromeleon 7 terminology is different from the terminology used in
Chromeleon 6.80. For details, refer to the 'Glossary - Chromeleon 7', which is
available in the Documents folder of the Chromeleon 7 installation.
• Diagnostic functions are not yet supported in Chromeleon 7.
For information about the general operating procedures and workflows in Chromeleon 7,
refer to the documents that are available in the Documents folder of the Chromeleon 7
installation and to the Chromeleon 7 Help.
5.1 Pump Diagnostics

All pumps except HPG-3200BX
Chromeleon 6.80 supports diagnostics functions for the pump. To run the diagnostics tests,
the Diagnostics Tool kit (part no. 6040.3099) is required. The kit includes all materials
required for performing the tests.
1. On the Control menu, select Diagnostics.

(The Control menu is visible only when a control panel is open.)
2. The Diagnostics dialog box lists all tests that are available for all devices in the
current timebase. Select a test for the pump. A wizard guides you through the test. For
more information about a test, see the Chromeleon Help.
To check the … Run the …

Pump system, especially the fitting connections, for General Leak Test*
leakage.
Pump valves and seals for leakage. The test helps you Detailed Leak Test*
to identify the source for the leak.
Overall performance of the pump, that is, pressure Performance Test
pulsation and compression.
Backpressure caused by the static mixer, inline filter, or Mixer Frit Test
the filter frit in the mixing system.
The vacuum of the degasser in a LPG-3400 or Degasser Vacuum Test
SRD-3x00 Solvent Rack
* Before running the leak tests, be sure that the Static Mixer property is set to the
correct value. You have to set the property also for the inline filter (ISO-3100, BM
pumps, and single-step mixing system). If the property is not set to the correct

value, the leak tests may not provide reliable results. Therefore, verify (for
example, in the Commands dialog box) that the property is set to the value
indicated on the mixer (inline filter). For the inline filter in LPG-3400BM and
DGP-3600BM pumps and in the single-step mixing system, select
InlineFilter_10µL on the list. For the inline filter in the ISO-3100BM, select
150µL.
If a test fails, check the Chromeleon Diagnostics Messages section for a short description
of possible causes along with recommended courses of action (→ page 137).
5.2 Predictive Performance and Service Interval

Chromeleon supports various functions for estimating the lifetime of consumables and
monitoring and recording service and (re)qualification information. The commands and
properties are available in the Commands dialog box and on the control panel for the
pump.
Tip: Predictive Performance is available also in Chromeleon 7.
On the control panel, wellness bars provide visual indicators of qualification and service
periods. The color-coding of the wellness bars provides information about the device
status:
Color Description
Green OK.
Yellow The value will soon reach the specified limit and/or the related component needs
servicing or should be replaced soon.
Orange Only for monitoring Qualification properties
The value has reached the specified limit. However, a Grace Period has been specified
during which the component can still be operated.
Red The value has reached the specified limit or the specified grace period has expired.
Replacement, servicing, or qualification of the component is overdue. The component
can no longer be operated and it is not possible to start a batch.
To keep the predictive performance information up-to-date, reset the related counter after
you have exchanged certain components (→ page 35). For more information about
predictive performance, see the Chromeleon Help. For information about the
recommended maintenance procedures and intervals, refer to the Maintenance Instructions
for the UltiMate 3000 Modules.
In addition, the seal workload can provide an indication as to when the pump should be
serviced. In Chromeleon, check the SealWorkLoad.Value property. As seal life depends on
the application and laboratory equipment, the following values can only be guiding values.
If SealWorkLoad.Value is higher than 8.0 MJ, the pump should be serviced. The value
corresponds to a workload of 10 months, 24 hours/day at a flow rate of 1 mL/min and a
backpressure of 20 MPa (2900 psi).

5.3 Service Commands and Properties

In addition to the pump commands and properties that are visible to the user in the
Commands (F8) dialog box under PumpModule (see the Chromeleon Help for details),
Chromeleon supports various commands and properties that are reserved for authorized
service personnel (→ table on page 22).
1. To see the service commands and properties in the Commands dialog box, change the
display filter level to Service (→ section 5.3.1)
2. Chromeleon can communicate certain service level commands and properties to the
pump firmware only when the pump is in the service mode. To activate the service
mode you have to enter a special service code (→ page 25).
5.3.1 Changing the Display Filter Level
To see the service level commands and properties in the Commands dialog box, you have
to change the display filter level.
IMPORTANT: Do not communicate these steps to the user.
Tip: The steps below are similar for Chromeleon 7.
1. In the Chromeleon Client program, simultaneously hold down CTRL+SHIFT and

select About Chromeleon on the Help menu to open the Restricted Area dialog.
Fig. 7: Restricted Area dialog box for Service level access
2. In the ID field, enter any character string. It must be a minimum of two characters and
be any combination of 0 - 9, a - z, and A - Z. The ID is not fixed and a different ID can
be used when you access the dialog box again in the future.
3. Make note of the five-digit number displayed at the bottom right corner of the dialog
box, here: 77762. This is a randomly generated number and will be different each time
the dialog box is accessed.
4. The password is created by alternately combining the characters used for the ID with
the reverse digits in the number code. To enter the password, type the first letter of the
ID, then the last number of the code, then type the second letter of the ID, then the

second to last number of the code. Continue in this pattern until all characters in the ID
and the number code have been entered. The ID is case sensitive.
Examples for the dialog box shown in Fig. 7:
Example 1 Example 2
ID Cookie Ok
Code 77973 12345
Code reversed 37977 54321
Password C2o6o7k7i7e O5k4321
5. On the Chromeleon control panel, open the Commands dialog box (press the F8 key
or select Commands on the Control menu).
6. Right-click in the devices list and select Service on the menu.
Fig. 8: Service level access

To see messages related to the service level properties in a specific Audit Trail, change
the display filter level to Service also in the Audit Trail. Note that this change will take
effect only for the specific Audit Trail. Important: Do not forget to change the display
filter level in the Audit Trail back to previous customer level, and then exit Chromeleon
to disable the Service level.
Tip: In the Commands dialog box, you need not change the display filter level back
to a customer level manually. Whenever you exit the Commands dialog box,
the filter level is automatically reset to Expert. However, to make the Service
level unavailable for selection on the menu, simultaneously hold down
CTRL+SHIFT and select About Chromeleon on the Help menu or exit the
Chromeleon Client program.

5.3.2 Service Level Commands and Properties
The service level commands and parameters that are available for the pump in the
Commands dialog box are grouped by hardware units and/or applicative tasks. They vary,
depending on the pump type, Chromeleon version, and options selected in the Properties
dialog for the pump. The table lists the service level commands and properties that are
important for service and repair work in the field. It does not include commands and
properties that are required for programming and debugging purposes at the factory or that
are reserved for future use.
The table lists the entries in alphabetical order, indicating the group in which the entry appears
at the end of the table. Most of these commands and properties are available also on the
service panel for the pump (→ page 26).
Tip: If the entries in the Commands dialog box are grouped by device hierarchy
(→ Fig. 8, page 21), consider to cancel the selection. All groups will then be
displayed on the same level, making it much easier to find the entry you are
looking for.
Chromeleon Description
_SendCommand1 Sends commands to the pump firmware, for example, to start the
brightness and contrast adjustment for the front panel display
(→ page 38).
CamZero2 The compensation value for the zero point of the compression start
reduces the pulsation of the pump block.
Note: With second-generation UltiMate 3000 pumps, it is no longer
necessary that you adapt this value after you have replaced a pump
block. The compensation value for the replacement pump block is
established at the factory and saved on the pump block board. The
value that comes with the replacement pump block and the values
available in the pump firmware and in Chromeleon are automatically
synchronized when the pump is turned on and/or connected in
Chromeleon for the first time after the replacement procedure.
CamRevolutions2 Reports the total number of pump strokes since the pump was used
for the first time. You cannot reset this counter.
CompressionMode2 The parameter is set at the factory to the pump type, for example,
HPG, and must not be changed.
With the factory setting, the firmware calculates the compression
automatically once per revolution and updates the related
Compression value (listed under Pump_Wellness_LeftBlock and
Pump_Wellness_RightBlock) accordingly.

DegasserType1 Reserved for pump programming at the factory:

• If the pump is a LPG-3400 with integrated degasser, the setting is
Internal.
• If a SRD-3x00 Solvent Rack with integrated degasser is operated
via the pump, the setting is External.
If the setting is not correct, check and change the Degasser Control
setting in the Properties dialog for the pump in the Server
FlowCalibration2 Reports the correction factor used for flow calibration. Do not
change this factor.
GetServiceCode1 Perform this command before you enter the service code. Only then,
the service code will take effect and set the pump into the service
mode. For more information, see page 25.
PressureFactor3 Sets the calibration factor for the pressure transducer in the purge
unit.
After you have replaced the purge unit, enter the value from the label
on the replacement purge unit.
For more information, see section 6.4.1 (→ page 37).
PressureOffset3 Reports the offset for the pressure transducer in the purge unit.
RearSealLeakCounter4 Reports how many drops per hour passed the main piston seals
between the past two wash cycles.
You can see the value also in the Audit Trail for the pump (Service
level only). The value may provide helpful information for
troubleshooting:
• If the value exceeds 100 drops per hour but the retention time
reproducibility of a peak is acceptable, the detector functionality
may be affected by the reasons indicated in the 'Messages on the
Pump Display' section. Check the following messages ' The rear seal
leak sensor detects drops constantly' (→ page 133) and 'The rear seal
wash system has run out of wash solution' (→ page 133). Possible
remedial actions are provided along with the reasons.
• Values between 20 and 100 drops per hour may indicate possible
leakage of the main piston seals. In the 'Messages on the Pump
Display' section, refer to the 'The piston seal leakage has exceeded
the recommended limit' message for remedial actions (→ page 131).
RearSealLeakCurrent4 Reports how many drops actually passed the main piston seals since
the last seal wash cycle.
RearSealWashPumpChanged1 Resets the counters for the rear seal wash pump (peristaltic pump)
and the rear seal wash tubing. Perform this command after you have
replaced the peristaltic pump.
ResetProportioningValveCounter 5 Resets the counter for the related proportioning valve to zero.
(Available with Chromeleon 6.80 Perform this command after you have replaced the proportioning
Service Release SR8 and later) valve. Before you perform this command, stop the pump (if it is
running).

SetPressureZero5 Calibrates the pressure transducer offset value.

Before executing this command, be sure that the system pressure is
down to 0 MPa. For more information, see section 6.4.2
(→ page 38).
ServiceCode1 Enter the service code to make the service mode available for the
pump. This is to allow Chromeleon communicate certain service
commands and properties to the pump firmware. For more
information, see page 25.
IMPORTANT: Do not communicate the service code to the user.
Make the service mode unavailable after you have performed
maintenance or repair work. To do so, set ServiceCode to
NoService or disconnect the pump in Chromeleon.
UpgradeInstalled1 Indicates and/or sets the pressure specification of the pump.
Yes—the pump is suitable for a maximum working pressure of
62 MPa (SD pumps, ISO-3100BM) or 103 MPa (RS pumps).
No—the pump is suitable for a maximum working pressure of
41 MPa (ISO-3100BM), 50 MPa (SD pumps, LPG-3400BM,
DGP-3600 BM) or 80 MPa (RS pumps)
1
Listed under [Main Device Name]_Service
2
Listed under [Pump Device Name]_Service_LeftBlock and/or [Pump Device Name]_Service_RightBlock
3
Listed under [Pump Device Name]_Pressure
4
Listed under [Main Device Name]
5
Listed under [Pump Device Name]_Service (visible only when the display filter level is Service)
The [Main Device Name] and [Pump Device Name] are specified in the Properties dialog for the pump on
the Devices page. The following names are the default names:
Pump Default Name

Main Pump Left Pump Right Pump
Device Name Device Name Device Name Device Name
ISO-3100 PumpModule Pump ----- -----
HPG-3x00
LPG-3400
DGP-3600 PumpModule ----- PumpLeft PumpRight
Be sure that you have selected the correct device before you perform a command.

5.3.3 Setting the Pump into the Service Mode
Chromeleon can communicate certain service level commands and properties to the pump
firmware only when the pump is in the service mode. A message appears when the pump is
not in the service mode. To activate the service mode, you have to enter the service code.
Tip: The following steps are similar for Chromeleon 7.
1. In the Commands dialog box, set the display filter level to Service (→ page 20).
2. Under PumpModule_Service, select GetServiceCode and click Execute.
3. Select ServiceCode, type 0009137 in the input field, overwriting the NoService entry,
and click Execute.
Use only this service code: 0009137.
IMPORTANT: Do not communicate this service code to the user.
Fig. 9: Service mode
Only one entry is possible at a time. If you have to repeat the entry, for example, due to a
typing error, you have to perform the GetServiceCode command again before you
reenter the service code. Only then, the service mode will be activated. (Note: You are
allowed to perform steps 1 and 2 three times in succession. If the third attempt fails, you
have to turn the pump off and on again to get another three attempts.)
Tips: In the Commands dialog box, you need not change the display filter level back
to a customer level manually. Whenever you exit the Commands dialog box,
the filter level is automatically reset to Expert. However, to make the Service
level unavailable for selection on the menu, simultaneously hold down
CTRL+SHIFT and select About Chromeleon on the Help menu or exit the
Chromeleon Client program.

If you have changed the display filter level to Service also in the Audit Trail,
you have to set the level back to the previous customer level manually.
Afterward, exit Chromeleon to disable the Service level.
You can set the pump into the service mode also from the service panel for the
pump (→ page 26).
5.4 Service Panel (Chromeleon 6.80 only)

Most commands and parameters that are required for service and repair work in the field
(→ table on page 22) are available also on the service panel for the pump. The service
panel is reserved for authorized service personnel. Do not make the service panel available
to the user.
The service panel (= UltiMate_3000_RS_SD_BM_BX_Pumps_Service.pan) is intended for

use with second-generation UltiMate 3000 pumps. For the first-generation UltiMate 3000
pump series, a separate service panel is available.
1. If necessary, restore the panel in an appropriate folder in Chromeleon. For

information, about how to restore files in Chromeleon, refer to the Chromeleon Help.
2. Double-click the panel.
3. In the Restricted Area dialog box, enter the ID and password, following the
appropriate steps in section 5.3.1 (→ page 20).
4. Connect the service panel to the timebase in which the pump is installed.
On the Control menu, select Connect to Timebase, and then select the timebase on
the Timebase tab. For information about the Timebase dialog, click Help.
Fig. 10: Service panel for the pump (here for a DGP-3600RS)

The service panel provides information about the pump status and allows you to make
parameter settings and perform commands directly from the panel.
For example, you can reset the predictive performance counters from the Service and
Wellness sub-panel (→ page 35):
1. Click Service and Wellness.

2. Click Log Replacement / Services to open the service checklists for the pump.
Fig. 11: Service panel for the pump (here for a DGP-3600RS)
3. Select the components that you replaced and click Continue.

Depending on the components you replaced, one or more messages remind you that you
should perform certain actions. If you performed these actions, click OK. The related
counters (if any) will be reset automatically.
Tip: If you have to set the pump into the service mode, click Hardware on the main
service panel, and then click Serv. Code on the Hardware sub-panel.
However, do not forget to the make the service mode unavailable again. To do
so, click Disable Service on the Hardware sub-panel.

6 Maintenance and Service

6.1 General Information and Precautions
Every six weeks, the pump automatically performs an internal maintenance procedure
when you initiate a purge cycle. While internal maintenance is running, the purge valve
must remain open. When internal maintenance is complete, the purge cycle starts
automatically. If no purge cycle is initiated after another six weeks, a message appears in
the Chromeleon Audit Trail, reminding you to start a purge cycle.
Chromeleon supports
• Diagnostic tests to check the performance of certain pump components (→ page 18)
• Functions for estimating the lifetime of consumables (→ page 19)
Observe the following general precautions before you begin:

• Be familiar with the general care and precautions in sections 2.1 and 2.2 (→ page 5 and
following pages).
• Do not operate the pump when the enclosure is open unless instructed to do so. Observe
all warning notes.
• The fluid components of the system may be filled with toxic solvents. Before starting
maintenance, rinse toxic solvents from the system. Put on protective clothing.
• Some capillaries of the RS and ISO-3100BM pumps and some Viper system capillaries
are made of MP35N, a nickel-cobalt based alloy. Individuals with sensitivity to
nickel/cobalt may show an allergic reaction from skin contact. Put on protective clothing
as appropriate.
• ISO-3100 and HPG-3200
Hydrostatic pressure builds up in the system when the fluid connections of the pump are
• Biocompatible pumps
♦ Do not use stainless steel frits with the biocompatible pumps because this would
render the biocompatibility void. Frits are used on the drawing side (in the solvent
reservoirs) and on the high-pressure side (in the inline filter).
♦ When connecting the solvent reservoirs, make sure that the appropriate frits are
installed on the end of the solvent supply lines. As a standard, filter holders with
stainless steel frits are provided in the accessories kit of the Solvent Rack. Replace
these frits with the frits from the accessories kit of the biocompatible pump. Note that

you need not replace the filter frits in the inline filter of the pump; they are titanium
frits as a standard.
♦ When substituting parts be sure to install the appropriate replacement part for the
biocompatible pump. For part numbers, see the related service sections in these
instructions and the Consumables and Spare Parts section (→ page 178).
♦ Ring seals with a titanium spring are installed as main piston seals in the
biocompatible pumps, whereas common ring seals with a steel spring are used in the
non-biocompatible pumps. Do not confuse these seals.
• All parts that are replaced or checked must be clean. Clean the parts with an appropriate
cleanser if necessary.
• To avoid damage to the module, do not clean the enclosure or any interior parts of the
instrument with compressed air.
• Do not forget to test the pump for leakage after you have carried out maintenance or
repair work on the fluid connections (→ page 110).
• For instructions on shutting down the pump, see page 123.
• When connecting capillaries, observe the information in section 6.2 (→ page 30).
• For information on the tools recommended for performing maintenance and repair, see
section 6.3 (→ page 33).

6.2 Connecting Capillaries

When connecting capillaries to the module, observe the following:
• Different fitting systems are used in an UltiMate 3000 system. Install the capillaries and
fittings only at the positions for which they are intended.
• Use only the capillaries shipped with the module and original spare capillaries.
• When you connect capillaries, make sure that the connectors are free from contaminants.
Even minute particles may cause damage to the system.
• Use Viper or nanoViper capillary connections whenever possible.
• Depending on the fitting connection, observe the following:
♦ Viper connections- depending on the Viper fitting:
Fig. 12: Viper fitting connections (examples)

(left: with knurled screw, right: with torque toothing)
Tips: You can reuse capillaries with Viper fitting connections also for a
different connection.
♦ Viper fitting with torque toothing (RS pumps)
When connecting these Viper fittings, keep the following in mind:
• Loosen or tighten these Viper connections only with your fingers and the
special installation tool (part no. 6040.2314). The installation tool is provided
in the accessories kit for the RS pumps (serial number ≥ 8063776).
• To avoid damage to the capillary or connection, tighten and loosen the Viper
connections only when the system pressure is down to zero.
• Mind the correct orientation of the tool when tightening or loosening
connections (see images further down).
Follow these steps:
1. To tighten a connection, insert the Viper capillary into the connection port.
2. Tighten the connection with your fingers.

3. Use the installation tool to tighten the connection further.
Fig. 13: Tool on torque toothing for tightening connections
4. Tighten the connection further until the torque limitation has been reached,
which is indicated by a clicking sound.
5. If the connection leaks:
a) Remove the capillary.
b) Clean the capillary ends carefully by using a cloth or tissue wetted with
isopropanol.
c) Reinstall the capillary.
d) If the connection continues to leak, install a new Viper capillary.
To loosen a connection, use the opposite side of the tool:
Fig. 14: Tool on torque toothing for loosening connections
♦ Viper fitting with knurled screw

When connecting these Viper fittings, keep the following in mind:
• Loosen and tighten these Viper connections only with your fingers and the
knurled screw . Do ot use tools other than the knurled screw that comes with
capillary. T
• To avoid damage to the capillary or connection, tighten and loosen the Viper
connections only when the system pressure is down to zero.
Follow these steps:
1. To tighten a connection, insert the Viper capillary into the connection port.
2. Tighten the connection by the knurled screw.
Note the slot in the knurled screw. You can easily remove the knurled screws
from neighboring capillaries through this slot and attach them again later.

No. Description
1 Knurled screw
2 Capillary
3 Slot
Fig. 15: Viper fitting with knurl
3. If the connection leaks:

a) Tighten the connection a little more.
b) If leakage continues, remove the capillary.
c) Clean the capillary ends carefully buy using a lint-free tissue wetted with
isopropanol.
d) Reinstall the capillary.
e) If the connection continues to leek, install a new Viper capillary.
♦ Conventional fitting connections (non-Viper)
When connecting capillaries with conventional fitting connections, observe the
following:
♦ Do not over tighten these fitting connections.
(The torque should not exceed 3 Nm for steel capillaries or 1.6 Nm for titanium
capillaries.)
♦ If you observe leakage on the connection, tighten a little further.
♦ If leakage still exists, first consider cleaning the connection port with a cleaning
swab (part no. 6040.0006). If this does not eliminate the problem, replace the
capillary and/or fitting.
♦ To avoid increased dead volume or damage to the system and leakage, reuse
fittings and ferrules only for the same capillary connection.

6.3 Recommended Tools

The following tools are recommended for performing maintenance and repair work on the
pump:
Description
Cleaning swabs
Grounding protection as appropriate
Installation tool for Viper capillaries with torque toothing
Multimeter
Piston seal (removal and insertion) tool
Pressure gauge (calibrated)
Pump head tool (spacing tool)
Screwdrivers
Hexagon wrench, sizes (mm) 2.5, 3.0, 5, and 6
Flat-blade screwdriver, sizes (mm) 0.6 x 3.5, 1, and 4
Open-end wrench, sizes 5 mm, 13 mm, and 1/4"
Phillips screwdriver, size PH0
Socket wrench, size 7 mm
Torx™ screwdriver, size TX 10
Torque wrench (optional)
Syringe, 12 mL
Vise to hold the pulse damper
The following maintenance kits are available for the pumps:
Maintenance Kit for Part No.

ISO-3100SD 6040.1950
ISO-3100BM 6042.1950
HPG-3x00SD 6040.1953
HPG-3x00RS 6040.1956B
HPG-3200BX (RP kit) 6042.1953
HPG-3200BX (NP kit) 6042.1954
LPG-3400SD 6040.1951
LPG-3400RS 6040.1954A
LPG-3400BM 6042.1951

Maintenance Kit for Part No.

DGP-3600SD 6040.1952
DGP-3600RS 6040.1955A
DGP-3600BM 6042.1952
For information about the content of the kits, see section 12 (→ page 178).

6.4 Required Parameter Updates or Adjustments

After you have replaced any of the following components, update the corresponding
parameter settings or make the required adjustment or alignment.
Important: Improper changes to the setup data will result in functional errors of the
pump. Consider the consequences before you change any values.
After replacing the … Perform the following action …

Front panel door Check and adapt the contrast and brightness of the front
panel display if necessary (→ page 38).
Leak sensor Adjust the leak sensor sensitivity if necessary (→ page 84).
Main board Check and adapt the screen contrast and brightness if
necessary (→ page 38).
Purge unit Enter the calibration factor for the pressure transducer in the
purge unit (→ PressureFactor, page 23 and section 6.4.1,
page 37).
Note:
With second-generation UltiMate 3000 pumps, you do not need to adapt the CamZero value
after you have replaced a pump block (→ page 22).
As part of the wellness and predictive performance features, Chromeleon provides various
counters. To keep the predictive performance information up-to-date, reset the counter for the
component you replaced or the action you performed:
After you have … Perform the following command

from the Commands dialog box …
Replaced the check valve CheckValveServiceDone1
Replaced the static mixer, inline filter, or MixerFritChanged2
filter frit in the mixing system or inline filter
(depending on the pump)
Replaced a piston PistonsChanged1
Replaced the main piston seal SealChanged1
Replaced the peristaltic pump RearSealWashPumpChanged4
(This command updates also the service
information for the peristaltic tubing.)
Replaced the peristaltic tubing RearSealWashTubeChanged3

After you have … Perform the following command

from the Commands dialog box …
Replaced the proportioning valve ResetProportioningValveCounter5
(Available with Chromeleon 6.80
Service Release SR8 and later)
Replaced the pump head CheckValveServiceDone1,
PistonsChanged1, SealChanged1,
SupportRingChanged1
Replaced the support ring SupportRingChanged1
Serviced the pump ServiceDone3
(for example, annual maintenance)
Performed instrument qualification QualificationDone3
1
Listed under Pump_Wellness_LeftBlock and/or Pump_Wellness_RightBlock
2
Listed under Pump_Wellness
3
Listed under PumpModule_Wellness
4
Listed under PumpModule_Service (visible only when the display filter level is Service)
5
Listed under Pump_Service (DGP-3600: PumpRight_Service and PumpLeft_Service)
(visible only when the display filter level is Service)
Tip: To make sure that all required actions are performed and the service counters
are reset, select the components that have been replaced in the Log
Replacement / Services dialog on the service panel for the pump (→ page 27).

6.4.1 Calibrating the Pressure Transducer for the System Pressure
The pressure transducer for the system pressure is integrated in the purge unit and needs to
be calibrated after the purge unit has been exchanged. To avoid the need for an extensive
calibration procedure in the field, calibration is performed at the factory. The calibration
factor is indicated on a label on the replacement purge unit. After you have exchanged the
purge unit, enter the calibration factor in Chromeleon.
1. In the Commands dialog box, click Pump_Pressure (for a DGP-3600:

PumpRight_Pressure and/or PumpLeft_Pressure), and then click PressureFactor
(→ page 23).
2. Type the value from the label on the purge unit in the PressureFactor box and click
Execute.
If you cannot use the value from the label, follow the steps below:
1. Enter the flow rate. Configure the system so that the backpressure is approximately
45 MPa.
2. Monitor the pressure with a calibrated pressure gauge. Connect the pressure gauge to
the pump, using a short capillary to ensure equal pressures on both sensors.
3. Wait until the pressure reading is stable.
4. Use the following formula to convert the pressure reading into the PressureFactor:
With:
Pressure Reading Pressure determined with the pressure gauge
Current Pump Pressure Value from the Pressure property in Chromeleon—the Pressure
property appears in the Commands dialog box under
PumpModule > Pump > Pump_Pressure.
Old PressureFactor value Value displayed in the PressureFactor field for the current pump
pressure
New PressureFactor value Value to be entered in the PressureFactor input field
Example: 59.7 MPa

× 80360 = 75909
63.2 MPa
5. Type the new value (here: 75909) in the PressureFactor box (→ page 23) and click
Execute.
6. Check to verify that the pressure reading and the current pump pressure show the same
value.

6.4.2 Setting the Offset for the Pressure Transducer (System Pressure)
The offset for the pressure transducer in the purge unit (PressureOffset, → page 23) is
balanced automatically when the pump is turned on. Turn on the pump while the current
system pressure is 0 MPa (= open purge screw). A typical value for the offset is between
2200 and 3100.
As an alternative, you can set the offset manually. To do so, perform the SetPressureZero
command from the Commands dialog box in Chromeleon (→ page 24).
6.4.3 Adapting the Brightness and Contrast of the Front Panel Display
If you install a new top cover including the front panel door and display, you may have to
adapt the screen contrast and brightness. Turn on the pump and check whether the screens
on the front panel display are clearly legible.
If they are, no further action is required. If they are not

1. Connect the pump in Chromeleon.
2. In the Commands dialog box, adapt the settings for Contrast and Brightness (both
listed under PumpModule) as required.
If the result is not yet satisfactory, adapt the basic limits from the Commands dialog box:
3. Under PumpModule_Service, select _SendCommand.
Fig. 16: Sending a command string

4. Type the string of set 1 into the input field (see table) and click Execute.
5. Under PumpModule, set Contrast and Brightness to the values indicated in the
table, and confirm each entry by clicking Execute.
6. Check the display. If the result is not yet satisfactory, repeat all steps with the next set.
Try the following sets:
Set In the _SendCommand field, Set Contrast Set Brightness

type the following string … to … to…
1 Display.Limits=15,75,184,200 50 50
2 Display.Limits=37,96,184,200 50 50
If these sets of strings are not satisfactory, contact your local Thermo Fisher Scientific
support center for the most recent values.

6.5 Removing and Installing the Side Panels

2. Remove the four screws that attach the side panels to the rear panel.
Remove these screws

Fig. 17: Screws attaching the side panels to the rear panel
3. Remove the screws that attach the side panels to the interior front panel. There is one
screw for each panel.
Remove these screws

Fig. 18: Screws attaching the side panels to the interior front panel (here HPG-3400SD)
4. To remove the side panel, tilt the upper edge of the panel slightly away from the
enclosure, and then lift the panel out of the enclosure and set it aside.
A square nut secures the screw that attaches the side panel to the interior front panel.
Interior front panel

Square nut securing
the side panel screw
Fig. 19: Square nut

When you remove the side panel, verify that the nut remains in place. If it does not, push
the nut back into the opening as far as it goes in. (Tip: To secure the nut until panel
reinstallation, consider screwing the panel screw back in again.)
5. To reinstall the side panel, follow the above steps in the reverse order. Observe the
following:
♦ Verify that the square nut for the side panel screw is in place.
♦ Insert the side panel in the openings provided between the rear panel and rear support
bar and between the front support bar and interior front panel.
♦ Be sure that the bracket on the inside of the side panel engages properly in the bottom
frame.
Fig. 20: Installing the side panel
6. Perform an electrical safety test according to national regulations.

6.6 Top Cover
6.6.1 Removing and Reinstalling the (Existing) Top Cover
1. Remove the side panels (→ page 40).

2. Remove the two screws that attach the top cover to the rear panel.
Remove these screws
Fig. 21: Screw attaching the top cover to the rear panel
3. Remove the screws that attach the top cover to the support frames. There are four
screws on either side of the pump.
Remove four screws on either side of the pump.

Fig. 22: Screws attaching the top cover to the support bars
4. Carefully lift and set the top cover to the left side of the pump (view from the front).
When setting the top cover aside, be careful not to pull on the display cable that still
connects the top cover to the main board. Be sure that the front panel door is closed.
This ensures that the top cover rests in a stable position.
5. Optional
If the top cover is in the way for further maintenance, remove the top cover completely as
described in section 6.6.2 (→ page 43).
6. To reinstall the top cover, follow the above steps in the reverse order.

6.6.2 Replacing the Top Cover
Description Part No.

Top cover (with front panel door & display) for
RS pumps 6040.1800
SD, BM, and BX pumps 6040.1810
1. Remove the top cover (→ section 6.6.1, page 42).

2. Unplug the ground cable from the screw that secures the cable on the mounting plate
for the main board.
3. Unplug the display cable from the connector on the main board.
4. Loosen the screw that secures the display cable on the mounting plate for the main
board, remove the cable, and retighten the screw.
Connector for the display

Loosen this screw and
cable on the main board
remove the display cable.
Ground cable
Fig. 23: Ground cable and display cable
5. To install the replacement top cover, follow the above steps in the reverse order.

7. Turn on the pump and check whether the screens on the front panel display are clearly
legible.
♦ If they are, no further action is required.
♦ If they are not, adapt the basic settings in Chromeleon. Follow the instructions on
page 38.

6.7 Replacing the Main Power Fuses

Fuse, 2 A, slow-blow, Included in the Fuses Kit, part no. 6030.9003
5x20 mm, 250V (→ section 12, page 178)
2. Remove the fuse cartridge, by using a small screwdriver.
Fuse cartridge
Fig. 24: Fuse cartridge

3. Replace the fuses with fuses of the appropriate rating. Always replace both fuses.
Warning: Always install two fuses. Use only the fuses indicated in the table.
4. Reinstall the fuse cartridge.

5. Reconnect the power cord to its source. Turn on the pump.

6.8 Purge Unit

The purge unit comprises the pressure transducer for the system pressure, purge valve, and
purge outlet nozzle.
Connection port for Connection port

the right pump head for the left pump head
(if available)
Pressure transducer for

the system pressure
Purge valve knob

Connection port (capillary mixer/
inline filter/pulse damper)
Purge outlet nozzle
Fig. 25: Purge unit
Only HPG-3x00
In addition, the purge unit combines the eluent streams from the pump heads.
6.8.1 Replacing the Purge Unit
Purge Unit for Part No.

HPG-3x00SD 6040.7155A
HPG-3x00RS, HPG-3200BX 6042.7157A
ISO-3100SD, LPG-3400SD, and DGP-3600SD 6040.7165A
ISO-3100BM, LPG-3400(RS, BM), and DGP-3600(RS, BM) 6042.7155A
Note:
The part numbers extended by an 'A' replace the part number without the 'A'. The 'A' purge units are fully
backward compatible. You can use them with all versions of the related pump type. Use purge units
without an 'A' in the part number only with the 50 MPa or 80 MPa pump versions.
If the pressure transducer for the system pressure is defective, replace the purge unit.
1. If necessary, purge the pump to remove toxic solutions.
2. Set the pump flow to 0. Wait until the system pressure is down.
3. Disconnect all tubing connections from the purge unit.
4. Unplug the pressure transducer cable from the P-Sys connector on the interior front
panel.

5. Remove the three screws that attach the purge unit to the interior front panel. Note that
the screws are of different lengths.
Unplug the pressure

transducer cable.
a
Remove three screws.
c b
Fig. 26: Screws attaching the purge unit
6. To install the replacement purge unit, follow the above steps in the reverse order.
Observe the following:
♦ Mind the screw lengths. Use the
Long screw for position a,
Short screw for position b
Medium length screw for position c (→ Fig. 26).
♦ Be sure that the pressure transducer cable is properly connected. If it is not, messages
relating to system overpressure may appear on the front panel display, for example,
'Can't reset pressure sensor. Pressure is out of range.'
7. Set the pressure transducer offset by performing the SetPressureZero command from
the Commands dialog box in Chromeleon (→ page 24).
8. In Chromeleon, update the calibration factor for the pressure transducer. To do so,
update the value in the PressureFactor box (→ page 23) with the value from the label
on the replacement purge unit.
9. Test the pump for leakage (→ page 110).

6.8.2 Replacing the Purge Valve Knob

Purge valve knob (with integrated cap seal) 6040.2035
Note:
Part no. 6040.2035 replaces part no. 6040.2030—the design of the purge valve knob has changed. The new
knob has an integrated cap seal, which is not subject to wear. The knob is fully backward compatible and
can be used in all purge units. (However, the earlier knob version (part no. 6040.2030) must be used only
with the 50 MPa or 80 MPa pump versions.)
Replace the purge valve knob if leakage is observed

• On the purge outlet when the valve is closed.
• Around the valve knob when the valve is open.
1. If necessary, purge the pump to remove toxic solutions.

2. Set the pump flow to 0. Wait until the system pressure is down.
3. To remove the purge valve knob from the purge unit, turn the knob counterclockwise
all the way, and then pull it straight off the purge unit.
Ring seal
Integrated cap seal
Fig. 27: Purge valve knob with integrated cap seal
4. Hold the (new) purge valve knob carefully. To avoid scratching the seals, do not hold
the knob by the seal end. Scratches will prevent proper sealing and allow leakage.
5. Before installing the purge valve knob, clean the hole in the purge unit with a cleaning
swab (part no. 6040.0006).
6. Insert the purge valve knob into the purge unit, turn the knob clockwise, and tighten
fingertight. Tighten with your hand only (use no tool). Overtightening may destroy the
purge valve seals.
7. Resume operation and check whether liquid leaves the purge outlet. If it does, the
knob may not be tightened enough. Tighten the knob a little more.

6.8.3 Replacing the Cap Seal
Relevant only for purge valve knobs without integrated cap seal

Cap seal (for purge valve knob 6040.2030 only) 6005.1301
Cap seal assembly tool 6300.0201

• The cap seal can be replaced only for purge valve knobs without integrated cap seal
(→ Fig. 28).
• Newer purge valve knobs (part no. 6040.2035) have an integrated cap seal (→ Fig. 27,
page 47) that cannot be replaced.
Cap seal Ring seal
Fig. 28: Purge valve knob—cap seal not integrated
Replace the
• Cap seal if leakage is observed on the purge outlet when the valve is closed.
• Purge valve knob if leakage is observed around the valve knob when the valve is open.
To replace the valve knob, follow the steps on page 47.
1. Remove the purge valve knob (→ page 47).
2. Remove the cap seal from the purge valve by using a small flat-head screwdriver.
3. Place the replacement cap seal into the cavity of the cap seal assembly tool.
4. Press the purge valve knob onto the cap seal at a right angle until the components lock
into place.
No. Description
1 Ring seal
2 Cap seal
Fig. 29: Cap seal assembly
5. Reinstall the purge valve knob (→ page 47).

6.9 Replacing the Check Valve Cartridges

The pump head contains two double check valves: an inlet valve and an outlet valve
(→ nos. 11 and 13 in Fig. 31, page 51 or Fig. 32, page 55).

Check valve cartridge, ceramics, 6041.2301
(The cartridge is the same for both the inlet valve and outlet valve.)
Valve nut kit
Stainless steel: RS pumps (S/N < 8030113) and SD pumps 6040.7007
Biocompatible: RS pumps (S/N ≥ 8030113), BM, and BX pumps 6042.7007
The kits include an inlet check valve nut and an outlet check valve nut.
1. If necessary, purge the pump to remove toxic solvents.

2. Set the pump flow rate to 0. Wait until the system pressure is down to zero.
3. Put on a pair of clean room gloves to prevent contamination on the valve parts.
Even minute particles may cause damage to the system and result in poor pump
performance.
4. Disconnect all tubing connections from the check valves. Observe the information
about the different fitting connections on page 30.
To remove the capillary on the outlet valve nut, consider disconnecting the other end of
the capillary (on the rear (lower) connection port) first to facilitate the procedure.
5. Loosen the check valve nuts, using an open-end wrench (size 13), and remove them
from the pump head.
6. Hold the nuts over your hand and turn them upside down, allowing the cartridges to
drop into your hand.
7. Insert a new cartridge into the check valve nut. Make sure that you insert the cartridge
in the direction of solvent flow, as indicated by the arrow on the cartridge.
8. Screw the check valve into the pump head and tighten the check valve (recommended
torque: 10 Nm). Do not overtighten to prevent damage to the cartridge.
Tighten the inlet check valve hand-tight and turn the bottom part so that the inlet opening
points backward (toward the interior front panel). Then, use an open-end wrench
(size 13 mm) to tighten the nut until the inlet opening is on the left (about one-quarter
turn).

Inlet valve (nut)
Inlet valve (bottom part)
Fig. 30: Inlet check valve
9. Reconnect the tubing connections to the inlet and outlet check valve nuts.
Inlet check valve
When you attach the solvent supply line to the inlet valve, take care to avoid cross
threading. If you observe leakage, tighten a little more. Do not overtighten.
Outlet check valve
To connect Viper capillaries: Observe the information about the Viper fitting
connections on page 30.
To connect conventional capillaries: On the outlet check valve, tighten the tubing hand-
tight, and then tighten it an additional one-quarter turn, using a wrench. If the connection
leaks, tighten little more. Do not overtighten; overtightening may damage the capillary.
10. To prevent contaminants from entering the HPLC system, thoroughly rinse the pump
(using at least 30 mL HPLC-grade water or purely organic solution). Open the purge
valve (with an ISO-3100 BM, observing the precautions on page 7) to prevent the
rinsing liquid from entering the HPLC system.
11. Test the pump for leakage (→ page 110). Tighten any leaking connections.
12. Update the service information in Chromeleon. Perform the CheckValveServiceDone
command.

6.10 Pump Head, Pistons, and Piston Seals

• Analytical and micro pumps, see further down
• Semipreparative pump: see page 55
Analytical and micro pumps
Fig. 31: Pump head, piston, and piston seals

(all pump types except HPG-3200BX)
Depending on the pump version, the representation of a part may be slightly different from
the real part. However, the position of the parts is the same for all pump versions.

No. Description Part No.
HPG-3x00RS HPG-3x00SD
Pump head, entire assembly, with: 6040.1901B1 6040.1903A2
1 Piston (sapphire) 6040.0042
2 Plate of the rear seal wash system -----
3 Piston seal in plate of the rear seal wash system 6040.0033
4 Seal ring (rear seal wash system) Included in 6040.2208
5 Rear seal wash body (pump head bushing) ----
6 Support ring of the piston seal 6040.0012
7 Main piston seal (reversed phase) 6266.0305 6040.0304
9 Pump head ----
10 Valve cartridge (same as no. 12) 6041.2301
11 Valve nut (inlet check valve) Included in:
HPG-3x00RS (S/N < 8030113) and HPG-3x00SD 6040.7007
HPG-3x00RS (S/N ≥ 8030113) 6042.7007 ---
13 Valve nut (outlet check valve) Included in:
HPG-3x00RS (S/N ≥ 8030113) 6042.7007 ---
14 Tubing connector for the seal wash tubing Included in 6040.9502
15 Capillary from working cylinder to equilibration cylinder
(U-tube Included in:
HPG-3x00RS (S/N ≥ 8030113) 6040.3002 ---
1
The part number extended by the 'B' replaces all previous part numbers (that is, those extended by an ‘A’
or without letter extension). The 'B' pump head is fully backward compatible. You can use the 'B' pump
head with all versions of the related pump type. Use pump heads without an 'A' or ‘B’ in the part number
only with the 80 MPa pumps.
2
The part number extended by the 'A' replaces the part number without the 'A'. The 'A' pump head is fully
backward compatible. You can use the 'A' pump head with all versions of the related pump type. Use
pump heads without an 'A' in the part number only with the 50 MPa pumps.
Tip: For SD pumps, normal phase seals are available for the main piston seal as an
option (part no. 6040.0306). Recommendation: When replacing reversed phase
seals with normal phase seals, also install a new piston. Changing the seals but
keeping the piston may impair the seal life.

LPG-3400RS ISO-3100SD LPG-3400SDN

DGP-3600RS LPG-3400SD DGP-3600SDN
DGP-3600SD
Pump head, entire assembly, with: 6040.1902B1 6040.1904A2 See Tip below
2 Plate (seal wash system) -----
3 Piston seal (in plate of seal wash system) 6040.0033 6040.0306
7 Main piston seal
depending on the pump type 6266.0305 6040.0304 6040.0306
Reversed Phase or Normal Phase (Reversed Phase) (Reversed Phase) (Normal Phase)

9 Pump head ----
11 Valve nut (inlet check valve) Included in:
RS pumps (S/N < 8030113); SD(N) pumps 6040.7007
RS pumps (S/N ≥ 8030113) 6042.7007 ---
11 Valve nut (outlet check valve) Included in:
RS pumps (S/N < 8030113); SD(N) pumps 6040.7007
RS pumps (S/N ≥ 8030113) 6042.7007 ---
15 Capillary from working cylinder to
equilibration cylinder (U-tube
RS pumps (S/N < 8030113); SD(N) pumps Included in 6040.3001
RS pumps (S/N ≥ 8030113) 6040.3003 ---
1
The part number extended by the 'B' replaces all previous part numbers (that is, those extended by an ‘A’
or without letter extension). The 'B' pump head is fully backward compatible. You can use the 'B' pump
head with all versions of the related pump type. Use pump heads without an 'A' or ‘B’ in the part number
2
The part number extended by the 'A' replaces the part number without the 'A'. The 'A' pump head is fully
backward compatible. You can use the 'A' pump head with all versions of the related pump type. Use
pump heads without an 'A' in the part number only with the 50 MPa pumps.
Tip: For SD pumps, normal phase seals are available for the main piston seal as an option (part no.
6040.0306). Recommendation: When replacing reversed phase seals with normal phase seals,
also install a new piston. Changing the seals but keeping the piston may impair the seal life.
SDN pumps
The pump head with part no. 6040.1904A can be used also with SDN pumps. However, this
pump head has reversed phase seals installed as main piston seals. Consider replacing the
reversed phase seals with normal phase seals (→ page 69).

LPG-3400BM
DGP-3600BM
ISO-3100BM
Pump head, entire assembly, with: 6041.1902
2 Plate of the rear seal wash system -----
3 Piston seal (in plate of seal wash system) 6040.0033
7 Main piston seal (reversed phase) 6266.0305
9 Pump head -- --
11 Valve nut (inlet check valve) Included in 6042.7007
13 Valve nut (outlet check valve) Included in 6042.7007
15 Capillary from working cylinder to equilibration cylinder (U-tube) Included in :
6042.3001 6042.3002

Semipreparative pump
Fig. 32: Pump head, piston, and piston seals (HPG-3200BX)

HPG-3200BX
Pump head, entire assembly, with: 6042.1901
1 Piston (ceramics) 6040.0842 (= 2 pistons)
2a Plate of the rear seal wash system ----
2b Sheet of the rear seal wash system ----
3 Piston seal (in plate of seal wash system) Included in 6040.9010
6 Support ring of the piston seal Included in 6040.9010
7 Main piston seal (reversed phase) Included in 6040.9010
8 Seal ring (rear seal wash system) ----
9 Pump head ----
11 Valve nut (inlet check valve) Included in 6042.7007
13 Valve nut (outlet check valve) Included in 6042.7007
15 Capillary from working cylinder to equilibration cylinder Included in 6042.3005
(U-tube)
Tip: Normal phase seals are available for the main piston seal as an option (part
no. 6040.9011). The kit includes 2 normal phase seals and 1 support ring.
Recommendation: When replacing reversed phase seals with normal phase
seals, also install a new piston. Changing the seals but keeping the piston may
impair the seal life.

6.10.1 Visually Inspecting the Pump for Piston Seal Leakage
You can inspect the pump visually for liquid leaks from the piston seals.
1. Flush the rear seal wash system with the seal wash solution.
a) Remove the seal wash tubing from the detector.
Seal wash tubing

Detector of the
seal wash system
Fig. 33: Tubing connection on the seal wash detector
b) Draw seal wash solution into a syringe at the free end of the tubing. Press the lever of
the peristaltic pump to the left so that the liquid can easily pass the system.
c) When about 2 mL of liquid are in the syringe, release the lever and remove the syringe
from the tubing.
2. Remove some liquid from the tubing, for example, by shaking the tubing.
3. To be able to produce the appropriate backpressure, install a flow resistance on the
pump outlet:
♦ All pumps (except BX pump):
Install the restrictor capillary (length: 15 m) from in the Diagnostics Tool Kit (part no.
6040.3099) to the pump outlet.
With the Viper union from the Diagnostics Tool Kit, connect the nanoViper capillary
(length: 750 mm, inner diameter: 75 µm, part no. 6041.5780) to the restrictor
capillary. Guide the free end of the capillary to a waste container.
♦ BX pump:
On the pump outlet, install a flow resistance, for example, a capillary that can produce
approximately 10 MPa at a flow rate of 1 mL/min. Guide the free end of the capillary
to a waste container.
4. Apply the appropriate flow rate (see table) to generate the backpressure indicated in
the table:
Pump Type Flow rate Backpressure

(approx.) (approx.)
RS pumps (suitable for max. working pressure)

80 MPa 1.5 mL 64 MPa
103 MPa 2 mL 80 MPa


(approx.) (approx.)
SD pumps (suitable for max. working pressure)

50 MPa 1 mL 40 MPa
LPG-3400BM, DGP-3600BM pumps

(suitable for max. working pressure)
50 MPa 1 mL 40 MPa
ISO-3100BM ( suitable for a max. working pressure)
BX pump 1 mL 10 MPa
5. To evaluate possible leakage, observe the liquid level in the tubing. Finish the
observation before a new seal wash cycle starts.
6. Depends on your observation:
♦ Liquid level in the tubing remains unchanged
The piston seals seal tightly. Reconnect the seal wash tubing to the seal wash detector.
(inner port, → Fig. 33). This completes the test.
♦ Liquid level in the tubing is decreasing
The seal wash seals are leaky. Continue with the next steps:
a) Replace the seal wash seals.
b) Reconnect the seal wash tubing to the seal wash detector (inner port, → Fig. 33).
c) Repeat the test starting with step 1.
♦ Liquid level in the tubing is increasing
One or more piston seals are leaky.
♦ LPG-3400 and ISO-3100 pumps:
a) Replace the piston seals in the pump head.
b) Reconnect the seal wash tubing to the seal wash detector (inner port,
→ Fig. 33).
c) Repeat the test starting with step 1.
♦ HPG-3x00 and DGP-3600 pumps:
Continue with the next steps.
7. On the right pump head, remove the seal wash tubing that connects the left pump head
to the right pump head.
8. Depends on the pump type:
♦ HPG-3x00 pumps
Apply the appropriate flow rate (see step 4) for 100 % of solvent A.

♦ DGP-3600 pumps
Apply the appropriate flow rate (see step 4) for the left pump.
9. Observe the liquid level in the tubing. Finish the observation before a new seal wash
cycle starts.
10. Depends on your observation:
♦ Liquid level in the tubing increases again
a) Replace the piston seals in the left pump head.
b) Reconnect the seal wash tubing to the right pump head.
c) Reconnect the seal wash tubing to the seal wash detector (inner port,
→ Fig. 33).
d) Repeat the test starting with step 1.
♦ Liquid level in the tubing does not increase again
a) Replace the piston seals in the right pump head.
b) Reconnect the seal wash tubing to the right pump head.
c) Reconnect the seal wash tubing to the seal wash detector (inner port,
→ Fig. 33).
d) Repeat the test starting with step 1.
Tip: If you observe leakage from a pump head, verify that the seal wash tubing is
properly connected to the pump head. If the tubing is not properly connected or
if the tubing is crimped or kinked, seal wash liquid may leak into the pump.

6.10.2 Pump Head and Pistons
6.10.2.1 Removing the Pump Head and Pistons
Tip: UltiMate 3000 pumps (except SDN and BX pumps) with yellow seals in the
plate of the rear seal wash system:
The seals are subject to wear. Whenever you remove the pump head, replace
the seals in the plate of the rear seal wash system.
1. Tilt the front cover upward.

2. If necessary, purge the pump to remove toxic solvents.
3. Set the pump flow rate to 0 and wait until the system pressure is down to zero.
4. Move the pistons into the appropriate position for the replacement procedure. To do
so, perform the appropriate command for the pump head that you want to remove:
♦ On the pump, perform the Change pump pistons, Change right pump pistons, or
Change left pump pistons command on the Control menu. Wait until the
Pump/Maintenance screen indicates that the pump head can be removed, before you
continue with the next steps.
—or—
♦ In Chromeleon, perform the UndockPistons command from the Commands dialog
box (listed under [Pump Device Name]_Wellness_RightBlock and/or [Pump
Device Name]_Wellness_LeftBlock; for information about the [Pump Device
Name], see page 24). Wait until the PistonPositionStatus property reports Undocked
before you continue with the next steps.
5. Undock the pistons of the second pump head if applicable.
6. Only HPG-3x00
Unplug the pressure transducer cable from the P-Work connector on the interior front
panel.
7. Disconnect all fluid connections from the pump head (→ Fig. 34, page 61). Note the
following:
• The U-tube on the pump head, connecting the working cylinder to the equilibration
cylinder, can remain connected.
• Disconnect the capillary to the purge unit also on the purge unit if necessary.
Observe the information about the different fitting connections on page 30.

Pressure transducer Seal wash tubing

cable
Seal wash tubing
Pump head outlet

Capillary from working
cylinder to equilibration Pump head screw
cylinder (U-tube)
Pump head inlet
Fig. 34: Pump head
8. Loosen (but do not yet remove) the pump head screw with a hexagon wrench
(size 6 mm).
9. Hold the pump head with one hand in its position, remove the pump head screw, and
then remove the pump head toward you.
The pistons are removed from the pump together with the pump head. Hold down the
plate of the rear seal wash system on the pump head and remove the pistons from the
head.
If the plate of the rear seal wash system remains in the pump when you remove the pump
head, follow these steps:
a) Set the pump head tool to the plate and carefully remove the plate from the pump
block.
♦ All pumps except HPG-3200BX
Hook in the tool at the upper edge of the plate.
Pump block tool hooked in Pump block

between plate and pump
block
Plate of the rear

seal wash system
Fig. 35: Pump head tool, hooked in
♦ HPG-3200BX
Hook in the tool at the lower edge of the plate.
b) HPG-3200BX
Verify that the sheet of the rear seal wash system (→ Fig. 32, no. 2b, page 55) has also
been removed from the pump.
c) Remove the pistons from the pump if necessary.

6.10.2.2 Installing the Pistons and Pump Head

Pump head (entire assembly)
HPG-3x00RS 6040.1901B1
HPG-3x00SD 6040.1903A2
HPG-3200BX 6042.1901
LPG-3400RS and DGP-3600RS 6040.1902B1
ISO-3100SD, LPG-3400SD, and DGP-3600SD 6040.1904A3
ISO-3100BM, LPG-3400BM, and DGP-3600BM 6041.1902
Notes:
1
The part number extended by the 'B' replaces all previous part numbers (that is, those extended by an
‘A’ or without letter extension). The 'B' pump head is fully backward compatible. You can use the 'B'
pump head with all versions of the related pump type. Use pump heads without an 'A' or ‘B’ in the part
number only with the 80 MPa pumps.
2
The part number extended by the 'A' replaces the part number without the 'A'. The 'A' pump head is
fully backward compatible. You can use the 'A' pump head with all versions of the related pump type.
Use pump heads without an 'A' in the part number only with the 50 MPa pumps.
3
This pump head has reversed phase seals installed as main piston seals. You can use the pump head
also with SDN pumps. However, in this case, consider replacing the reversed phase seals with normal
phase seals (→ page 69).
Piston
All pumps except HPG-3200BX (2 pistons, sapphire) 6040.0042
HPG-3200BX (2 pistons, ceramics) 6040.0842
1. Only BX pump
Place the sheet of the rear seal wash system onto the plate of the rear seal wash system.
Mind the correct orientation.
Plate (rear seal wash

system)
Sheet (rear seal wash

system)
Fig. 36: Rear seal wash system: plate and sheet (HPG-3200BX)
2. Place the plate of the rear seal wash system onto the pump head.
3. Inject a few drops of isopropanol into the piston cavities and insert the pistons.
4. Use the pump head tool (spacing tool) to establish the correct distance between the
piston and the plate of the rear seal wash system.

Place the spacing tool on the plate of the rear seal wash system and push both pistons
downward as far as possible (→ Fig. 37, page 63). The distance is correct when both
pistons touch the spacing tool. Remove the tool.
Pump head tool (here for SD and RS pumps)
Fig. 37: Establishing the distance
5. Place the assembly back into the pump and tighten the pump head screw with a
hexagon wrench (size 6 mm; recommended torque: 10 Nm).
If you removed both pump heads , do not interchange them during reinstallation.
♦ HPG-3x00: Note the label on the pressure transducer cable: A identifies the left pump
head; B identifies the right head.
♦ DGP-3600: Note the label (L or R) on the capillary from the working cylinder to the
equilibration cylinder (U tube).
Tip: Replacement pump heads do not have a label. For easy identification for
future maintenance, label the replacement pump heads as required.
6. Connect the fluid connections on the pump head. Observe the following:
♦ When installing the capillary from the pump head to the purge unit, be careful not to
bend the capillary. Install the capillary in the direction of solvent flow. Note that one
side of the capillary is longer than the other is. Connect the longer side to the pump
head. With some capillaries, the direction of the flow is indicated by an arrow on the
capillary.
♦ When you attach the solvent supply line to the inlet valve, avoid cross-threading.
7. Only HPG-3x00
Reconnect the pressure transducer cable to the P-Work connector on the interior front
panel.
Verify that the cable is properly connected. If it is not, messages relating to the
working pressure may appear on the front panel display, for example, 'Working piston
pressure out of range'.
8. Return the pistons into the appropriate position for normal operation. To do so,
perform the appropriate command for the pump head that you want to install:
♦ On the Pump/Maintenance screen on the pump display, select Dock. Wait until the
screen returns to the Main menu before you continue with the next steps.
—or—

♦ In Chromeleon, perform the DockPistons command from the Commands dialog box
(listed under [Pump Device Name]_Wellness_RightBlock and/or [Pump Device
Name]_ Wellness_LeftBlock; for information about the [Pump Device Name], see
page 24). Wait until the PistonPositionStatus property reports Operational before
you continue with the next steps.
9. Flush the pump head with seal wash solution (→ step 1 on page 57).
10. Rinse the pump thoroughly to prevent contaminants from entering the HPLC system.
♦ Rinse the pump with at least 30 mL of HPLC-grade water or pure organic solution.
♦ Open the purge valve (with an ISO-3100 BM, observing the precautions on page 7) to
prevent the rinsing liquid from entering the HPLC system.
11. Test the pump for leakage (→ page 110). Tighten any leaking connections.
12. Update the service information in Chromeleon. Perform the PistonsChanged
command for the pump head for which you replaced the pistons (→ page 35).
If you have replaced the entire pump head, also perform the SealChanged,
CheckValveServiceDone, and SupportRingChanged commands.

6.10.3 Replacing the Piston Seals
The piston seals prevent solvent from leaking into the rear seal wash system or into the
pump. This may cause unstable flow rates and baseline noise.
Each piston has two piston seals. One seal is in the pump head (→ no. 7 in Fig. 31, page 51
and/or in Fig. 32, page 55), the other seal is in the plate of the rear seal wash system (→ no. 3
in Fig. 31 and/or Fig. 32).
The replacement procedure for the piston seals in the pump head consists of
1. Removing the pump head and pistons (→ page 60)
Tip: UltiMate 3000 pumps (except SDN and BX pumps) with yellow seals in the
plate of the rear seal wash system:
The seals are subject to wear. Whenever you remove the pump head,
replace the seals in the plate of the rear seal wash system.
2. Disassembling the pump head and removing the piston seals (→ page 66)
3. Cleaning the pistons (→ page 68)
4. Reassembling the pump head (→ page 69)
5. Reinstalling the pistons and pump head (→ page 62)
6. After you have replaced the piston seals, observe the recommendations on page 72.
If you want to replace the piston seals in the plate of the rear seal wash system, follow the
steps on page 74.
Tip: Except for the SDN pumps, the pumps are shipped with reversed phase piston
seals as main piston seals.
Keep in mind that using chloroform, trichlorobenzene, methylene chloride,
tetrahydrofuran, or toluene as solvents chemically damages the UHMW-PE
seals. Chemical reactions may also occur when using tetrachloromethane,
diethyl ether, di-isopropyl ether, ketones, ammonium hydroxide,
methylcyclohexane, and monochlorobenzene.
If the customer wants use these solvents, contact your sales representative for
HPLC Products.
The SDN pumps are shipped with normal phase piston seals (NP) as main
piston seals. For SD and BX pumps, normal-phase piston seals are available as
an option (→ page 69). Depending on the application, the abrasion may be
slightly increased. Therefore, check the permeability of the static mixer or
inline filter (depending on the pump type) at regular intervals.

6.10.3.1 Disassembling the Pump Head and Removing the Piston Seals
1. Remove the pump head and pistons as described in section 6.10.2 (→ page 60).
Tip: Never disassemble the pump head with bare hands. Put on a pair of clean
room gloves to prevent contamination of the pump parts. Even minute
particles may cause damage to the system and result in poor pump
performance.
2. Remove the plate and the sheet of the rear seal wash system if applicable.
Plate (rear seal wash system)
Rear seal wash body

(pump head bushing)
Piston
Support ring
Rear seal wash body (pump head bushing)
Plate (rear seal wash system)

Sheet (rear seal wash system)
Rear seal wash body
(pump head bushing)
Piston
Support Ring
Rear seal wash body (pump head bushing)

Fig. 38: Pump head removed
(top: all pumps except HPG-3200BX; bottom: HPG-3200BX)
3. Remove the rear seal wash body (pump head bushing) from the pump head.
4. First, remove the support ring.
Tap the pump head on a surface to make the support ring drop out. If this does not
remove the support ring, push the seal insertion tool into the pump head and remove the
tool. The support ring is removed together with the tool.
Tip: Do not use the seal removal tool for the support ring because the ring can
be removed from the tool only with difficulties.

5. Remove the piston seal from the pump head.

Push the seal removal tool (→ Fig. 39, page 67) into the pump head as far as it goes in.
Seal removal tool Seal insertion tool
Fig. 39: Piston seal removal and insertion tool
6. Remove the tool from the pump head. The piston seal is removed together with the
tool. The piston seal will be destroyed and cannot be reused.

6.10.3.2 Cleaning the Pistons

Cleaning the pistons is required only if you reinstall the pistons you removed. Verify that the
pistons are clean and free of damage.
Sapphire piston only: Hold the rear side of the piston into the light. Dirt particles will be
enlarged by the refraction of the light.
Tip: Even if the piston seems to be clean, consider performing the fingernail test in
addition. Hold the piston and carefully move your fingernail over the surface as
shown in the picture. After performing this test, you have to clean the piston
again.
The piston is clean when the surface is completely smooth (no inconsistent or
rough areas).
Fig. 40: Fingernail test (here on a sapphire piston)
To clean the piston, use common household abrasive powder. Use only powder and be sure
that it does not contain waxy additives.
1. Depending on the pump type, moisten the sapphire or ceramics area of the piston.
2. Apply dry abrasive powder on a lint-free tissue and add few drops of water until you
obtain a paste similar consistency.
3. Rub the piston with this paste.
4. First, rinse the piston with plenty of clear, cold water.
5. Rub the piston several times with a clean, lint-free cloth or tissue.
6. Verify that
♦ The cleanser is completely removed.
♦ The piston is free of damage. To do so, repeat the light test and/or fingernail test.
If the piston is scratched or scored, install a new piston.
7. Rinse the piston thoroughly with isopropanol.
8. Rub the piston several times with a dry, lint-free cloth or tissue.
9. Install the piston as described on page 62.

6.10.3.3 Installing the Piston Seals and Reassembling the Pump Head

Main piston seal
(→ no. 7 in Fig. 31, page 51 and/or Fig. 32, page 55)
RS pumps (Reversed Phase) 6266.0305
SD pumps (Reversed Phase) 6040.0304
SDN pumps (Normal Phase) 6040.0306
BM pumps (Reversed Phase) Included in 6025.2012
BX pump (Reversed Phase) Included in 6040.9010*
SD and BX pumps only
Normal phase seals are available for the main piston seal as an
option:
SD pumps (normal phase seals) 6040.0306
BX pumps Included in 6040.9011
Support ring (→ Fig. 31 or Fig. 32, no. 6) for
RS pumps, SD pumps, and BM pumps (ceramics ring) 6040.0012
BX pump (PEEK ring) Included in 6040.9010*
* The part numbers include 2 piston seals and 1 support ring.
Tip: When replacing reversed phase seals with normal phase seals (or at any time
later vice versa) also install a new piston. Changing the seals but keeping the
piston may impair the seal the seal life.
Tip: Never reassemble the pump head with bare hands. Put on a pair of clean room
gloves to prevent contamination of the pump parts. Even minute particles may
cause damage to the system and result in poor pump performance.
1. Depending on the support ring

Ceramics support ring—RS and SD pumps and BM pumps
The ceramics support ring is not a wear part. Thus, you need not replace the support ring
when you replace the piston seal. However, before reinstalling the support ring, clean the
support ring from the inside and outside, using isopropanol, a cleaning swab (part no.
6040.0006), and a lint-free cloth or tissue.
Tip: 50 MPa SD pumps and BM pumps were shipped with non-ceramic
(PEEK) support rings as a standard. If the pump for which you want to
replace the piston seal has a PEEK support ring installed, replace the
PEEK support ring with the ceramics support ring.

PEEK support ring—BX pumps

With these pumps, always replace the support ring whenever you replace the piston seal.
2. Pipette a few drops of isopropanol onto the edge in the pump head on which the piston
seal will rest.
3. First, slide the support ring, and then slide the piston seal onto the seal insertion tool
(→ Fig. 39, page 67). Mind the correct orientation of the seal. The open side of the
seal must face away from the tool.
This side faces away

from the tool.
Fig. 41: Correct orientation of the piston seal (here reversed phase)
4. Push the piston seal tool into the pump head as far as it goes in.
5. Remove the tool from the pump head. The piston seal and the support ring remain in
the pump head.
Fig. 42: Seal installed in the pump head
6. Insert the rear seal wash body (pump head bushing). Mind the correct orientation
(→ Fig. 43). The even side faces the pump head; the opposite side faces the plate of
the rear seal wash system.
This side faces This side faces the plate of

the pump head. the rear seal wash system.
Fig. 43: Orientation of the rear seal wash body

7. Place the plate of the rear seal wash system onto the pump head as shown in the
picture, with the channels in the plate facing you.
Fig. 44: Pump head with plate of the rear seal wash system
(left: all pumps except HPG-3200BX, right: HPG-3200BX)
8. Only HPG-3200BX
Place the sheet of the rear seal wash system onto the plate of the seal wash system as
shown in the picture. Mind the correct orientation.
Fig. 45: Pump head with plate and sheet of the rear seal wash system
9. Install the pistons and pump head (→ page 62).

10. After replacing the piston seals, observe the recommendations on page 72.
11. Update the service information in Chromeleon for the pump head for which you
replaced the piston seals and/or support rings. Perform the SealChanged command for
the piston seals and the SupportRingChanged command for the support ring
(→ page 35).

6.10.3.4 Recommended Actions after Main Piston Seal Replacement

Observe the following recommendations when you have replaced the main piston seals in the
pump head:
Tip: Pump performance and the seal life cycle depend directly on the actions
recommended in this section. Therefore, always perform these actions after you
replacing the piston seals.
• Allow new piston seals to run in.
a) Connect drain tubing to the purge outlet nozzle (if it is not yet connected).
b) Open the purge valve (with an ISO-3100 BM, observing the precautions on page 7).
c) Depending on the pump type:
Install the restrictor capillary (length: 15 m) from in the Diagnostics Tool Kit
(part no. 6040.3099) to the pump outlet.
With the Viper union from the Diagnostics Tool Kit, connect the nanoViper
capillary (length: 750 mm, inner diameter: 75 µm, part no. 6041.5780) to the
restrictor capillary. Guide the free end of the capillary to a waste container.
♦ BX pump:
On the pump outlet, install a flow resistance, for example, a capillary that can
produce approximately 10 MPa at a flow rate of 1 mL/min. Guide the free
end of the capillary to a waste container.
d) Operate the pump. For 15 minutes, apply a flow rate of 1.3 mL/min (BX pump:
10 mL/min), using isopropanol. Do not deliver in circles.
e) Close the purge valve.
f) Depending on the pump type:
For 30 minutes, apply the flow rate (see table) using isopropanol to generate
the backpressure indicated in the table:

(approx.) (approx.)
RS pumps (suitable for max. working pressure)

103 MPa 2 mL 80 MPa
SD pumps (suitable for max. working pressure)
50 MPa 1 mL 40 MPa


(approx.) (approx.)
LPG-3400BM, DGP-3600BM pumps

(suitable for max. working pressure)
50 MPa 1 mL 40 MPa
ISO-3100BM ( suitable for a max. working pressure)
♦ BX pump:
For 30 minutes, operate the pump at a flow rate of 10 mL/min using
isopropanol.
g) Turn off the pump flow and wait until the system pressure is down to zero.
h) Remove the capillary from the pump outlet and reconnect the pump to the system.
i) Purge the pump using the solvent of your application.
j) If an increased leakage rate is observed with new piston seals after hours of operation,
operate the pump for at least 2 hours at 35 MPa (BX pump: 10 MPa).
If the piston seals are not sufficiently tight yet, have the pump build up high pressure
(→ page 110) and hold the high pressure for some minutes.
k) When removing the tubing from the purge outlet nozzle, place a tissue under the outlet
to absorb any liquid that might be leaving the outlet when the tubing is removed.
l) Check the permeability of the filter frit, depending on the pump type, in the mixing
system (→ page 87) or inline filter (→ page 96).
• Never run the pump dry. Damage to the pistons and the piston seals could result.

6.10.3.5 Replacing the Piston Seals in the Plate of the Seal Wash System

Piston seal in the plate of the seal wash system
(→ no. 3 in Fig. 31, page 51 and/or Fig. 32, page 55)
RS, SD, and BM pumps 6040.0033
SDN pumps 6040.0306
BX pump Included in 6040.9010
The replacement procedure depends on the pump type:
• All pumps except HPG-3200BX: Follow the steps further down on this page.
• HPG-3200BX: Follow the steps on page 75.
All pumps except HPG-3200BX
Tip: UltiMate 3000 pumps (except SDN pumps) with yellow seals in the plate of the
seal wash system:
The seals are subject to wear. Whenever you remove the pump head, replace
the seals in the plate of the seal wash system.
1. Remove the pump head and pistons (→ page 60).
2. Remove the plate of the rear seal wash system.
3. Set the seal insertion tool to the side of the seal (on the low-pressure side of the plate)
as shown in the picture and push the seal out of the plate.
Fig. 46: Removing the seal from the plate of the seal wash system

4. Insert and push the replacement seal into the plate (high-pressure side) with your hand.
To prevent contamination, put on a pair of gloves.
With black seals: Mind the correct orientation of the seal. The open side of the seal faces
away from the plate.
The open side faces

away from the plate
Fig. 47: Inserting the piston seal into the plate of the rear seal wash system
(left: black seals (P/N 6040.0306), yellow seals (P/N 6040.0033)
5. Place the plate of the seal wash system back onto the pump head.
6. Reinstall the pistons and pump head (→ page 62).
HPG-3200BX
1. To remove the pump head and pistons, follow the steps in section 6.10.2.1
(→ page 60).
2. Remove the sheet and plate of the seal wash system.
3. Set the seal insertion tool to the side of the seal (on the high-pressure side of the plate)
and push the seal out of the plate.
Fig. 48: Removing the seal from the plate of the seal wash system
4. Slide the new piston seal onto the seal insertion tool. Mind the correct orientation of
the seal. The closed side of the seal must face the tool.
5. Insert the replacement seal into the plate (low-pressure side).
Fig. 49: Inserting the piston seal into the plate of the rear seal wash system

6. Place the plate and the sheet of the rear seal wash system onto the pump head. Mind
the correct orientation.
Fig. 50: Plate and sheet of the rear seal wash system
7. Install the pistons and pump head (→ page 62).

6.11 Rear Seal Wash System
6.11.1 Inspecting the Rear Seal Wash System for Leakage
1. Remove the seal wash tubing from the detector.
Seal wash tubing

Detector of the
rear seal wash system
Securing clip
Fig. 51: Detector of the seal wash system
2. Draw seal wash solution into a syringe at the open end of the tubing. Press the lever of
the peristaltic pump to the left so that the liquid can easily pass the system.
3. Press the lever of the peristaltic pump firmly onto the tubing, and then press liquid into
the seal wash system from the syringe.
4. Inspect the tubing and connections of the seal wash system for indications of leakage.
Tighten or replace leaking connections as necessary.
6.11.2 Replacing the Detector

Detector of the seal wash system 6040.4131
If the seal wash solution is isopropanol with 0.1% sulfuric acid (to ensure
that the liquid is conductive) as is recommended for NP applications, use
the detector from the appropriate Normal Phase (NP) kit (for SD(N)
pumps: part no. 6040.1972, BX pump: part no. 6040.1975).
1. Remove the seal wash tubing from the detector (→ Fig. 51).
2. Unplug the detector cable from the Drop Det connector on the interior front panel.
3. Remove the securing clip for the detector toward you, and then remove the detector
toward to top.
4. Install the replacement detector following the above steps in the reverse order.
Connect the tubing to the inner port (→ Fig. 51). The outer port has no function.

6.11.3 Cleaning the Detector Electrodes

The electrodes in the seal wash detector can be contaminated by deposits impairing the
correct functioning of the seal wash system.
1. Remove the detector as described in section 6.11.2 (→ page 77).

2. Clean the electrodes with distilled water and wait until the detector is dry. The
electrodes are critical for proper operation. Therefore, be careful not to bend or
otherwise damage them during cleaning.
Electrodes
Fig. 52: Detector of the seal wash system
3. Reinstall the detector.

6.11.4 Replacing the Peristaltic Tubing

Peristaltic tubing (= PharMed™ tubing, white) from the
Tubing Kit for the Rear Seal Wash System 6040.9502
1. Remove the seal wash tubing from the seal wash reservoir to avoid liquid spill.
2. Remove the peristaltic tubing from the peristaltic pump. Press the lever to the left,
remove the tubing from the pump, and release the lever.
Tubing from seal

Tubing
wash reservoir
connector
Peristaltic tubing
(PharMed tubing)
Rotor
Tubing connection
Lever on the pump head
Fig. 53: Peristaltic pump
3. Disconnect the peristaltic tubing from the connection port on the pump head.
4. Disconnect the other end of the peristaltic tubing from the seal wash tubing (clear
tubing).
5. Locate the PharMed tubing (white tubing) in the Tubing Kit for the Rear Seal wash
system. Cut the tubing to the required length.
6. Connect the replacement tubing to the connection port on the pump head and to the
tubing from the seal wash reservoir. Push the PharMed tubing onto the connector as
far as it goes on (a small collar should be visible).
7. Engage the tubing in the peristaltic pump. Press the lever to the left, place the tubing in
the pump, and release the lever.
8. Update the service information in Chromeleon. Perform the
RearSealWashTubeChanged command (→ page 35).

6.11.5 Replacing the Peristaltic Pump

Peristaltic pump 6040.1150
1. Remove the seal wash tubing from the seal wash reservoir to avoid liquid spill.
2. Remove the peristaltic tubing from the peristaltic pump. Press the lever to the left,
remove the tubing from the pump, and release the lever.
3. Remove the top cover (→ page 42).
4. Unplug the control cable for the peristaltic pump from the pump block board.
Unplug the peristaltic

pump cable.
Fig. 54: Connector for the peristaltic pump
5. Remove the screw that attaches the ground cable for the peristaltic pump in the
enclosure and remove the cable. Be careful not to lose the washers and contact discs.
(There are two washers and two contact discs.)
Remove the ground cable.
Fig. 55: Ground cable for the peristaltic pump

6. Remove the two screws that attach the peristaltic pump to the front support bar.
Remove two screws.
Fig. 56: Screws attaching the peristaltic pump
7. Lift and remove the peristaltic pump from the enclosure.

8. To install the replacement peristaltic pump, follow the above steps in the reverse order.
Before attaching the ground cable, arrange the washers, cable lug, and contact discs,
and nut in the correct order (as indicated in the picture), and then tighten the screw.
Fig. 57: Components for attaching the ground cable
9. Recommendation
After replacing the peristaltic pump, d also replace the peristaltic tubing (white tubing). If
necessary, also replace the seal wash tubing (clear tubing). Cut the tubing shipped with
the peristaltic pump to the required length and install as required.
10. Update the service information in Chromeleon. Perform the
RearSealWashPumpChanged command (→ page 35). Note that this command also
updates the service information for the peristaltic tubing.

6.12 Leak Sensor
6.12.1 Drying the Leak Sensor (Eliminating Leakage)
The leak sensor is installed inside the pump. The sensor reports a leak when liquid collects
in the drip tray under the fluid connections. Locate the leak, eliminate the cause, and dry
the leak sensor.
Fig. 58: Leak sensor
1. Wait until the pressure is down to zero.

2. Inspect the fluid connections in the pump for signs of leakage. Tighten or replace
leaking connections.
3. With a cloth or tissue, absorb all liquid that has collected in the tray. Be careful; do not
bend or damage the sensor.
4. Allow the sensor to adjust to the ambient temperature for a few minutes. The red sensor
LED should become dark. (The yellow LED may be dark or illuminated; this LED does
not indicate an error.)
5. If no errors are reported, you can resume operation.
6. Test the pump for leakage after you have carried out maintenance or repair work on the
fluid connections (→ page 110).
Tip: If the sensor is not dry, the Status LED remains red. To remove the message
from the pump display, select Clear on the navigation bar.

6.12.2 Replacing the Leak Sensor

Leak sensor kit 6040.0009

2. Disconnect all fluid connections between the pump blocks and on the solvent selector
if applicable.
3. Remove both pump blocks (including fluidics and interior front panel) from the
enclosure:
a) Unplug the control cable for the pump block from the connector on the pump block
board (→ Fig. 76, page 104).
b) For the left pump block, unplug also the control cables for the peristaltic pump and
leak sensor. With a LPG-3400, also unplug the cable for the pump light on the right.
c) Remove the four screws that attach each pump block to the enclosure and disconnect
the ground cable (→ Fig. 79, page 105).
d) Lift and remove the pump block assembly from the enclosure.
4. Remove the two screws that attach the leak sensor in the enclosure.
Remove two screws.
The sensor must not touch

the enclosure bottom.
Fig. 59: Screws attaching the leak sensor
5. Lift to remove the leak sensor from the enclosure.

6. To install the replacement leak sensor, follow the above steps in the reverse order.
♦ The leak sensor must not touch the enclosure bottom. If it does, the pump will report
leakage although no leak is present. The distance between the enclosure bottom and
sensor is correct if you can easily push a sheet of paper under the sensor.
♦ When reinstalling the pump block, be sure that the ground cable is connected properly.
7. Turn on the pump.
8. Adjust the leak sensor sensitivity (→ page 84).

6.12.3 Adjusting the Leak Sensor Sensitivity

2. Allow the leak sensor to warm up and stabilize for 3 minutes (minimum).
Tip: For proper adjustment, the sensor must be dry and installed properly.
Avoid draft (for example, by an open window) as this may impair the
adjustment.
3. Adjust the sensor sensitivity on the potentiometer, using a small ceramics screwdriver
(for example, flat-blade and size 0.4 x 2.0 mm).
Turn the potentiometer until the yellow LED is just off (that is, by a fraction of a turn in
the opposite direction the LED is illuminated).
Yellow LED
Red LED
Potentiometer
Fig. 60: Leak sensor
♦ To reduce the sensitivity, turn the potentiometer clockwise.

♦ To increase the sensitivity, turn the potentiometer counterclockwise.

6.13 Mixing System

RS pumps, SD pumps (except ISO-3100SD) and HPG-3200BX
The steps described in the following sections refer to the replacement procedure for the
mixers with which the pump is shipped:
• All RS and SD pumps (except ISO-3100SD) are shipped with a two-step mixing system
that includes a capillary mixer (volume: 50 µL) and a static mixer (volume either 150 µL
or 350 µL).
In addition, mixing systems with other volumes are available. For details, see section 10
(→ page 173).
• The HPG-3200BX is shipped with a static mixer (volume 750 µL) and a capillary that
connects the mixer to the purge unit.
6.13.1 Replacing the Capillary Mixer and/or Static Mixer

Capillary mixer, stainless steel (volume: 50 µL) for the following
SD pumps and RS pumps (serial number < 8030113)
HPG-3x00 6040.3015
LPG-3400 6040.3026
DGP-3600 6040.3025
Capillary mixer, Viper, MP35N (volume: 50 µL) for the following
RS pumps (serial number ≥ 8030113)
HPG-3x00RS 6042.3015
LPG-3400RS 6042.3026
DGP-3600RS 6042.3025
Static mixer, volume: 350 µL, for
HPG-3x00SD, LPG-3400 and DGP-3600 (both SD and RS) 6040.5310
(This static mixer replaced the previous 350 µL static mixer with part no. 6040.5350.)
HPG-3x00RS 6040.5110
(This static mixer replaced the previous 150 µL static mixer with part no. 6040.5151.)
HPG-3200BX 6040.5750
Important: The design of the current static mixers does not allow opening the
mixer housing for any reason.

1. When replacing one or both mixers, observe the following:

♦ Observe the direction of flow through the static mixer.
♦ When you loosen or tighten capillaries on the static mixer, hold the mixer with an
open-end wrench to prevent the mixer from turning in the mounting bracket.
♦ Observe the information about the different fitting connections on page 30.
2. Disconnect the outlet capillary from the static mixer.

3. Disconnect the capillary mixer from the purge unit.
Purge Unit
Static Mixer
Capillary mixer
Fig. 61: Capillary mixer (here in an HPG-3400SD)
4. Remove the static mixer from the mounting bracket by pulling the mixer toward you.
5. Remove the mixer assembly from the pump.

6. Disconnect the capillary mixer from the static mixer.
7. The following steps are required only when replacing the previous 350 µL static mixer
(part no. 6040.5350) with the current 350 µL static mixer (6040.5310)
In this case, a different mounting bracket must be installed. The bracket is shipped with
the mixer.
a) Loosen the two screws that attach the mounting bracket for the mixer (mind the
orientation of the bracket; the orientation is important when you install the new
bracket) and remove the bracket. Keep the screws; you will need them to install the
new bracket.
b) The mixer ship kit includes two different brackets: one bracket for installing the mixer
in HPG-3x00SD or RS pumps and one bracket for installing the mixer in LPG-3400
or DGP-3600 pumps. Locate the mounting bracket that looks similar to the one you
removed.
c) Attach the new mounting bracket to the interior front panel of the pump (same
position and orientation as the one you removed) by using the two screws from the
bracket you removed.

8. Connect the (new) capillary mixer to the (new) static mixer.

9. Insert the mixer assembly into the pump.
10. Insert the static mixer in the mounting bracket.
11. Connect the capillary mixer to the purge unit.
12. Connect the outlet capillary to the static mixer.
13. When the static mixer has been replaced or exchanged
♦ Update the service information in Chromeleon. Perform the MixerFritChanged
command.
♦ In Chromeleon, verify that the StaticMixer property is set to the correct value. This is
especially important when a mixer with a volume different from the volume of the
mixer that was removed has been installed. If the property is not set to the correct
value, the leak tests (→ page 18) may not provide reliable results.
6.13.2 Checking the Static Mixer for Permeability
Check the static mixer for permeability at regular intervals.
Tip: You can check the permeability of the static mixer also by running the Mixer
Frit Test from Chromeleon (→ page 18).
1. Disconnect the capillary on the outlet of the static mixer.

2. Have the pump deliver water at a flow rate of 2 mL/min.
When the outlet is open, the pressure should be less than 0.5 MPa.
3. If required
Replace the static mixer (→ page 85).
4. Reconnect the capillary to the outlet of the static mixer.

6.13.3 Replacing the Filter Frit in the Previous Static Mixers
This section is relevant only for the previous static mixers (part numbers 6040.5350 and
6040.5151). To distinguish them from the current mixers, note that they have no pressure
specification imprinted on the housing.

Filter frit kit with
2 frits (stainless steel, porosity: 10 µm) and 2 sets of seals for
Static mixer, mixing volume: 150 µL, 6035.0016
(which was previously available with part no. 6040.5151)
Static mixer, mixing volume: 350 µL 6040.0040
(which was previously available with part no. 6040.5350)
Important: These filter frits and seals must be used only with static mixers
indicated in the table. The design of other mixers does not allow
opening the mixer housing for any reason.
The steps and pictures refer to the replacement procedure for the filter frit in the static
mixer (mixing volume: 350 µL), but are the similar for the 150 µL static mixer.
Tip: Whenever you replace the filter frit, also replace the two seals.
1. Disconnect the capillaries on the inlet and outlet of the static mixer.
2. Remove static the mixer from the mounting bracket by pulling the mixer toward you.
3. Open the mixer housing, using two open-end wrenches (size 13 mm).
4. Remove the two seals and filter frit.
5. Close the empty mixer housing with your hands and mark any position on the housing.
Fig. 62: Mixer housing with position mark
This mark is important when you close the mixer housing after you have inserted the
replacement filter frit and sealing rings.

6. Open the mixer housing.

7. Insert the replacement filter frit and the sealing rings.
Interchanging the filter frit and sealing ring in the mixer housing (outlet) will damage the
seals, and thus impair the mixer functionality, when you reassemble the mixer housing.
Follow these steps:
1 2 3 4 5
Fig. 63: Mixer housing with filter frit and sealing rings
No. Description
1 Mixer housing (outlet)
2 Filter frit
3 Sealing ring (small)
4 Sealing ring (large)
5 Mixer housing (inlet)
The order in which the seals are installed is important:

a) Inspect the inside of the mixer housing for indications of contamination. Clean the
housing in an ultrasonic bath if necessary.
b) First, insert the filter frit into the mixer housing (outlet).
c) Then, place the small sealing ring onto the filter frit. Make sure that the ring rests flat
on the frit (→ Fig 64, page 89).
Fig 64: Filter frit and sealing ring inserted in the mixer housing (outlet)

d) Push the large sealing ring onto the mixer housing (inlet).
Fig. 65: Large sealing ring inserted in the mixer housing (inlet)
The mixer housing (inlet) has a round flange. The sealing ring must be behind this
flange.
Flange
Sealing ring
Fig. 66: Mixer housing (inlet) with sealing ring (side view)
8. Close the mixer housing with your hand until you encounter resistance. The mark on
the mixer housing should be approximately in the position shown in the picture.
Fig. 67: Mixer housing with position marks

9. Close the mixer housing, using the two open-end wrenches (size 13 mm) until the
marks on the housing are in a line (→ Fig. 62, page 88). (Recommended torque:
10 Nm)
10. Insert the static mixer into the mounting bracket and reconnect the capillaries to the
mixer inlet and outlet.
12. Update the service information in Chromeleon. Perform the MixerFritChanged
command.

6.14 Inline Filter
6.14.1 Inline Filter (ISO-3100)
6.14.1.1 Replacing the Inline Filter

ISO-3100SD and ISO-3100BM
Inline filter (volume: 150 µL) 6040.5110
(This inline filter replaced the previous 150 µL inline filters with part nos. 6040.5151 and 6042.5016.)
ISO-3100SD
Capillary from purge unit to inline filter 6040.3024
ISO-3100BM
Capillary from pulse damper to inline filter Included in 6042.3002
Important: The design of the current inline filter does not allow opening the
housing for any reason.
1. Disconnect the capillary on the filter inlet and filter outlet.

When you loosen or tighten capillaries on the inline filter, hold the filter with an open-
end wrench to prevent the filter from turning in the mounting bracket.
Fig. 68: Inline filter
2. Remove the inline filter from the mounting bracket by pulling the filter toward you.
3. Insert the replacement inline filter into the bracket and reconnect the capillaries to the
filter inlet and outlet.
command.
5. In Chromeleon, verify that the StaticMixer property is set to 150 µL. If the property is
not set to the correct value, the leak tests (→ page 18) may not provide reliable results.

6.14.1.2 Replacing the Filter Frit in the Previous Inline Filter
This section is relevant only for the previous inline filters (part numbers 6040.5151 and
6042.5016). To distinguish them from the current filters, note that they have no pressure
specification imprinted on the housing.

Filter frits (porosity: 10 µm) for inline filter ISO-3100SD
Kit including 2 filter frits and 2 sets of seals 6035.0016
The frits and seals must be used only with the previous inline filter (part
no. 6040.5151).
Filter frits (titanium, biocompatible, porosity: 0.2 µm) for inline filter
ISO-3100BM 6042.3335
Kit including 2 filter frits and 2 sets of seals
The frits and seals must be used only with previous inline filter (part no.
6042.5016).
Important: These filter frits and seals must be used only with inline filters indicated
in the table. The design of other filters for the ISO-3100 pumps does not
allow opening the housing for any reason.
The replacement procedure for the filter frit in the inline filter is the same as for the filter
frit in the previous static mixer (→ page 88).

6.14.2 Inline Filter (LPG-3400BM, DGP-3600BM)
6.14.2.1 Replacing the Inline Filter

Inline filter for LPG-3400BM and DGP-3600BM 6042.5014
(volume: 10 µL)
Capillary from purge unit to inline filter 6042.3024
(LPG-3400BM, DGP-3600BM)
Tip:
The capillary is included also in the capillary kit for the LPG-3400 and DGP-3600 (part
no. 6042.3001). For information about the kit content, see section 12 (→ page 178).
1. Disconnect the capillaries on the filter inlet and outlet and remove the inline filter from
the pump.
Fig. 69: Inline filter
2. Connect the capillaries to the replacement inline filter (observing the direction of flow
through the filter as indicated by an arrow on the filter) and place the filter under the
pump head in the pump enclosure.
3. In Chromeleon, verify that the StaticMixer property is set to InlineFilter_10 µL. If
the property is not set to the correct value, the leak tests (→ page 18) may not provide
reliable results.

6.14.2.2 Replacing the Filter Frit in the Inline Filter

Filter frits for inline filter of LPG-3400BM and DGP-3600BM 6268.0036
(2 frits, porosity: 2 µm)
1. Remove the inline filter as described in section 6.14.2.1.

2. Open the inline filter and remove the filter frit.
3. Pipette a drop of solvent into the frit holder and insert the replacement frit. Close the
filter housing. The solvent keeps the frit in the holder during closing.
4. Install the inline filter as described in section 6.14.2.1.
command.

6.14.3 Checking the Inline Filter for Permeability
Check the permeability of the inline filter at regular intervals.
Tip: You can check the permeability of the inline filter also by running the Mixer
Frit Test from Chromeleon (→ page 18).
1. Disconnect the capillary on the filter outlet.

2. Have the pump deliver water at a flow rate of 2 mL/min.
When the outlet is open, the pressure should be less than 0.5 MPa.
3. If required
♦ ISO-3100
Replace the inline filter (→ page 92).
♦ LPG-3400BM or DGP-3600BM
Replace the inline filter (→ page 94) or the frit in the filter (→ page 95).
4. Reconnect the capillary to the outlet of the inline filter.

6.15 Replacing the Solvent Selector or Proportioning Valve

Solvent selector "2 from 4" (HPG-3400) 6040.2611
3-channel proportioning valve (DGP-3600) 6040.4026
4-channel proportioning valve (LPG-3400) 6040.4025

2. Disconnect the tubing connections from the solvent selector or proportioning valve.
3. HPG-3x00 only
On the left pump head, disconnect the capillary that connects the pump head to the purge
unit.
4. Depending on whether you want to replace the left or right solvent selector or
proportioning valve, disconnect the tubing of seal wash system on the related pump
head, and unplug the drop detector cable from the connector on the interior front panel
if necessary.
5. HPG-3x00 and LPG-3400 only
On the purge unit, disconnect the capillary that connects the static mixer to the purge unit
and remove the static mixer from the mounting bracket.
6. Unplug the cables indicated in the picture from the pump block board as necessary:
Control cable for the pump block
Peristaltic pump Leak sensor LPG-3400 only:

Pump light
Solvent selector or proportioning valve
Fig. 70: Connectors on the pump block boards

7. If the pump has a fan installed

Unplug the fan cable from the main board (→ Fig. 3, page 13), remove the screws that
attach the fan in the pump, and remove the fan.
8. Remove the screws that attach the pump block to the enclosure bottom. There are two
screws on either side of the pump block.
Ground cable
Remove two screws on
Remove two screws on the left of the block.
the right of the block.
Fig. 71: Removing the pump block screws and ground cable (here right pump block)
9. Unplug the ground cable on the pump block and lift the pump block out of the
enclosure.
10. Remove the two screws that attach the solvent selector or proportioning valve to the
interior front panel.
Remove two screws.
Fig. 72: Removing the attachment screws (here for a solvent selector)
11. To install the replacement solvent selector or proportioning valve, follow the above
steps in the reverse order. Observe the following:
♦ When installing a 4-channel proportioning valve
On the replacement valve, note the label for channel A. When installing the valve, be
sure that channel A of the replacement valve is installed in the port labeled A on the
interior front panel.

♦ Be careful when inserting the pump block into the enclosure. Avoid damage to the
grounding angles on the interior front panel.
Grounding angle
Fig. 73: Grounding angle on interior front panel
♦ Do not forget to reconnect the ground cable to the pump block.

♦ When reconnecting the solvent supply lines to the solvent selector or proportioning
valve, observe the labeling on the interior front panel.

13. Thoroughly rinse and purge the pump. Check the connections for indications of
leakage. Tighten leaking connections as necessary.
14. Proportioning valve only
Update the service information in Chromeleon. Perform the
ResetProportioningValveCounter command. The counter is available with
Chromeleon 6.80 Service Release SR8 and later.

6.16 Replacing the Main Board

Main board 6040.0201
The main board can be used with all second-generation UltiMate 3000 pumps.
3
2
1
1
1 1
Fig. 74: Main board
No. Description
1 Main board screws—five screws attach the main board to the mounting plate
2 Grounding angles on rear panel ports
3 Solvent Rack port
Important: If you have to replace the main board and a pump block, you have to
observe the following order of steps:
1. First, replace the main board. Perform all steps of the main board
replacement procedure below (only step 11can be omitted.)
2. Only then, replace the pump block (→ page 103). Perform all steps
of the replacement procedure.
1. Verify that firmware version 3.00 or later (for the BX pump: 3.21 or later) is installed
in the pump. Update the firmware if necessary (→ page 153).
3. On the rear panel of the pump, remove the two screws next to the Solvent Rack port.
4. Unplug all cables from their connectors on the main board (→ Fig. 4, page 14).

5. Recommendation
The replacement main board is shipped with two control cables for the pump blocks.
Therefore, unplug the control cables also from the connectors on the pump block boards.
Use the cables from the ship kit when installing the replacement main board.
6. Remove the five screws that attach the main board to the mounting plate.
7. Pull the main board away from the rear panel (be careful not to bend or otherwise
damage the grounding angles on the rear panel ports) and lift the board out of the
enclosure.
8. To install the replacement main board, follow the above steps in the reverse order, but
do not install the top cover yet. Observe the following:
♦ To avoid damage to the Solvent Rack port on the rear panel, first tighten the screws
next to the Solvent Rack port on the rear panel, and then tighten the screws that attach
the main board to the mounting plate.
♦ Use the control cables shipped with the replacement main board to connect the pump
blocks to the main board.
♦ LPG-3400 pumps only
If the data cable for the degasser was connected on the main board that you removed
(→ Fig. 4, page 14), remove the other end of the cable on the degasser vacuum pump.
Connecting the cable is no longer required with main boards revision B and later.
9. Turn on the pump. Wait until the pump has finished booting.
The pump has not yet the correct serial number. Turn the pump off and on again. The
pump will now start up with the correct serial number.
10. A message appears on the front panel display, indicating that a new hardware
configuration has been detected. The pump-specific data, such as, the calibration
values, is synchronized with the data on the pump block board. Afterward, you can
test the pump for 48 operating hours.
A—If you have to replace only the main board
♦ If the test operation is not satisfactory, remove the main board within 48 hours.
♦ If the test operation is satisfactory, no further action is required if you need to replace
only the main board. After 48 hours, the data will be saved permanently to the main
board.
B—If you have to replace also a pump block
♦ Perform the following sequence of actions to save the data permanently immediately:
♦ In the Commands dialog box, under PumpeModule_Service, click
_SendCommand.
♦ In the CommandString input box, type AcceptHardwareConfig and
confirm the entry by clicking Execute.

For A and B, keep the following in mind:

♦ Once the pump-specific data have been saved permanently to the main board, this
main board can no longer be used as a replacement main board in another pump.
Therefore, if you want to run the pump with the replacement main board only for test
purposes, remove the main board within 48 hours.
♦ If you install a main board to which data of a different pump have been saved, the
pump will run only for 48 hours and will then stop operation automatically.
11. Reinstall the top cover (→ page 42).

12. Check whether the screens on the front panel display are clearly legible.
If they are, no further action is required.
If they are not, adapt the basic settings in Chromeleon (→ page 38).

6.17 Replacing a Pump Block

Pump block (without fluidics) for
RS pumps 6040.5020
SD pumps 6040.5030
BM pumps 6040.5040
BX pump 6040.5050
Important: If you have to replace the main board and the pump block, you have to
observe the following order of steps:
1. First, replace the main board. Perform all steps of the main board
replacement procedure (→ page 100; only steps 11 and 12 can be
omitted.)
2. Only then, replace the pump block. Perform all steps of the
replacement procedure as described below.
1. Verify that firmware version 3.0 or later (for the BX pump: 3.21 or later) is installed in
the pump. Update the firmware if necessary (→ page 153).
3. Depends on the pump type
Disconnect the tubing connections from the solvent selector, proportioning valve, or
inlet valve.
4. Depends on the pump block you want to replace
♦ If you want to replace the right pump block (view from the front)
Remove the right pump head (→ page 60).
Remove the purge unit (→ page 45).
♦ If you want to replace the left pump block (view from the front)
Unplug the drop detector cable from the connector on the interior front panel.
Remove the left pump head (→ page 60).
Remove the static mixer from the mounting bracket by pulling the mixer toward you.
Remove the two screws that attach the mounting bracket to the interior front panel.
Remove these screws.
Mounting bracket
Static mixer
Fig. 75: Static mixer (here in an HPG-3x00SD)

5. Unplug the following cables from the pump block board (→ Fig. 76, page 104). All
other cables should remain connected.
♦ For the right pump block
Unplug the control cable for the pump block and the cable for the solvent selector or
proportioning valve if applicable.
♦ For the left pump block
Unplug the control cable for the pump block and the cables for the solvent selector or
proportioning valve, if applicable, peristaltic pump, and leak sensor.
With a LPG-3400 pump, also unplug the cable for the pump light.
Control cable for the pump block
Peristaltic pump Leak sensor LPG-3400 only:

Pump light
Solvent selector or proportioning valve
Fig. 76: Connectors on the pump block boards
6. Remove the pump light. To do so, loosen the screw on the light board and remove the
board, screw, and distance spacer.
Remove this screw.
Fig. 77: Pump light (view from above)

7. Remove the screws that attach the pump block to the interior front panel. There are
two screws for the left pump block (HPG-3x00: three screws) and two screws for the
right pump block.
For the right pump block, the screw on the bottom right of the pump block is already
removed when you removed the purge unit.
Left pump block in

Remove these screws. a HPG-3x00 only:
Also, remove this screw.
Fig. 78: Screws attaching the pump block (here right block)
8. Remove the interior front panel. Keep the interior front panel for installation of the
replacement block.
9. If the pump has a fan installed
a) Unplug the fan cable from the main board (→ Fig. 3, page 13).
b) Remove the screws that attach the fan in the pump and remove the fan.
10. Remove the screws that attach the pump block to the enclosure bottom.
Ground cable
Remove these screws
Remove these screws on on the left of the block.
the right of the block.
Fig. 79: Removing the pump block screws and ground cable (here right pump block)
11. Unplug the ground cable on the pump block and lift the pump block out of the
enclosure.
12. To install the replacement pump block, follow the above steps in the reverse order, but
do not connect the control cable for the pump block yet. Observe the following:
♦ Attach the replacement pump block to the interior front panel that you removed from
the defective block.

♦ Be careful when inserting the pump block into the enclosure. Avoid damage to the
grounding angles on the interior front panel.
Grounding angle
Fig. 80: Grounding angle on interior front panel
♦ Do not forget to connect the ground cable to the replacement pump block.
13. Depends on the serial number of the pump and on whether you replaced one or both
pump blocks
♦ Serial number of the pump > 8013800 and one pump block replaced
Connect the control cable for the pump block, turn on the pump, and continue with the
next step.
♦ Serial number of the pump > 8013800 and both blocks replaced
Connect the control cable for only one of the replacement pump blocks and turn on the
pump. The following message appears on the pump display: 'Connection error:
Reconnect pump block to main board.' Turn off the pump, connect the control cable
for the second pump block, and turn on the pump again. Continue with the next step.
♦ Serial number of the pump ≤ 8013800
Independent of whether you replaced one or both pump blocks: Turn on the pump
with the control cable for only one pump block being connected. The following
message appears on the pump display: 'Connection error: Reconnect pump block to
main board.' Turn off the pump, connect the control cable for the second pump block,
and turn on the pump again. Continue with the next step.
14. A message appears on the front panel display, indicating that a new hardware
configuration has been detected. The pump-specific data, such as the calibration
values, is synchronized with the data on the main board. Afterward, you can test the
pump for 48 operating hours.
♦ If the test operation is not satisfactory, remove the pump block within 48 hours.
♦ If the test operation is satisfactory, no further action is required.
After 48 hours, the data will be saved permanently to the pump block.

Keep the following in mind:

♦ Once the pump-specific data have been saved permanently to the pump block, this
block can no longer be used as a replacement pump block in another pump.
Therefore, if you want to run the pump with the replacement pump block only for test
purposes, remove the pump block within 48 hours.
♦ If you install a pump block to which data of a different pump were saved, the pump
will run only for 48 hours and will then stop operation automatically.

Note: With second-generation UltiMate 3000 pumps, you need not lubricate the cams
manually. Lubrication is performed automatically at regular intervals.

6.18 Replacing the Switching Power Supply

Switching power supply (24V/150W) 6820.2820
1. Turn off the pump, remove all cables connected to the rear panel, and place the pump
onto the rear panel.
2. Hold the bottom plate with one hand and remove the four screws that attach the
bottom plate for the switching power supply to the pump bottom. Be careful not to
lose the contact discs.
Remove four screws to

remove the bottom plate
from the pump bottom.
Fig. 81: Bottom plate
3. Carefully pull the bottom plate away from the pump bottom. Turn the bottom plate so
that you can reach the switching power supply.
4. Disconnect all terminal connections on the switching power supply.
Fig. 82: Terminal connections on the switching power supply

5. Turn the bottom plate, hold the switching power supply with one hand, and remove the
two screws that attach the switching power supply to the bottom plate.
Remove two screws to remove

the power supply unit from the
bottom plate.
Fig. 83: Bottom plate
6. To install the replacement power supply, follow the above steps in the reverse order.
To avoid personal injury or damage to the instrument:
♦ Make sure to connect all cables to the correct terminals as shown in the Fig. 82. Be
sure that they are secured properly in the terminals. This is important for the
operational safety of the pump.
♦ Be sure that the line voltage cables are secured in a cable tie. This is important for the
operational safety of the pump.
♦ When tightening the bottom plate to the pump bottom, be sure that the contact discs
are in place.

6.19 Testing the Pump for Leakage

After you have carried out maintenance or repair work on the fluid system, test the pump
for leakage.
Tips: With a DPG-3600, you have to perform the test twice, that is, separately for
each of the two pumps.
You can test the pump for leakage also with the diagnostic functions in
Chromeleon (→ page 18).
1. Depends on the pump type

♦ All pumps except ISO-3100BM
Close the pump outlet with an appropriate fitting plug.
♦ Only ISO-3100BM
Connect the restrictor capillary from in the Diagnostics Tool Kit (part no. 6040.3099)
to the pump outlet and close the other end of the capillary with a fitting plug.
2. In Chromeleon, set the upper pressure limit, under [Pump Device Name] > Pressure
> UpperLimit. (For information about the [Pump Device Name], see page 24.)
Pump Type Upper Pressure Limit
RS pumps, suitable for a maximum working pressure of

80 MPa 60 MPa
103 MPa 80 MPa
SD pumps, suitable for a maximum working pressure of
50 MPa 40 MPa
62 MPa 50 MPa
BM pumps
LPG-3400BM and DGP-3600BM 40 MPa
ISO-3100BM, suitable for a maximum working pressure of
41 MPa 40 MPa
62 MPa 50 MPa
BX pump 15 MPa
3. In Chromeleon, select a flow rate of, for example, 50 µL/min (ISO-3100BM:

500 µL/min).

4. Decrease the flow as soon as the pressure builds up (see table).
Pump Type Typical Pressure

80 MPa between 40 and 50 MPa
BM pumps
LPG-3400BM and DGP-3600BM between 10 and 20 MPa
ISO-3100BM (41 MPa and 62 MPa types) between 25 and 30 MPa
BX pump between 10 and 13 MPa
5. Have the pump deliver some µL/min until the following pressure has built up:
Pump Type Pressure

80 MPa 55 MPa
103 MPa 75 MPa
50 MPa 35 MPa
62 MPa 45 MPa
BM pumps
LPG-3400BM and DGP-3600BM 35 MPa
ISO-3100BM, suitable for a maximum working pressure of
41 MPa 35 MPa
62 MPa 45 MPa
BX pump 14 MPa
6. When this pressure has been built up and when the pump delivers a master flow of
1 µL/min (SDN pumps: 4µL/min; ISO-3100BM: 10µL/min), the pressure should
increase or remain constant at least. If it does not, this indicates possible leakage.
7. Find and eliminate the cause for the leak, and then perform the test described below
this list. Possible sources are:
♦ Capillary connections
Inspect the connections for signs of leakage and tighten leaking connections.
♦ Piston seals
♦ Inspect the piston seals for leakage (→ page 57).
♦ If leakage continues, replace the piston seals (→ page 65).
♦ If leakage is observed with new piston seals, operate the pump for at least
2 hours at 35 MPa (HPG-3200BX: 5 MPa) to run in the seals. Note: In this
case, tightening the pump head screw more does not help.

♦ Check valves
♦ Tighten the check valve nuts.
♦ Remove and clean the check valve nuts (for example, in an ultrasonic bath).
♦ Purge valve
Inspect the purge valve knob and purge unit for indications of leakage.
Test
a) Pressure up the pump. This is the best way to identify a leaking connection.
b) Allow 5 minutes for the pump to stabilize. This is important as the pressure drops
faster during the first 5 minutes, because the seals and other components have to
adjust to the pressure.
c) After the stabilization time, monitor the pressure drop.
d) Tighten the connection a little more. The pressure will suddenly increase a little.
e) Monitor if the pressure drops at the same rate as before. If the pressure drops
significantly slower, the connection was leaking.
8. In Chromeleon, reset the upper pressure limit to the value used before the leak test.
Tip: If leakage from a pump head is observed, check also the tubing connected to
the rear seal wash system. If the seal wash tubing is not connected properly or
if the tubing is crimped or kinked, the liquid may leak into the pump.

6.20 Front Panel Door
6.20.1 Replacing the Front Panel Display

Front panel display 6820.0213

2. Remove the screws that attach the outside cover plate of the front panel. There are 8
screws.
Fig. 84: Screws on the inside of the front panel
3. Tilt the front panel upward and remove the outside cover plate by pulling it toward the
front.
Fig. 85: Removing the outside of the front panel
4. Remove the screw that attaches the display to the front panel.
Display cables
Display screw
Fig. 86: Attachment screw for the display
5. Disconnect the display cables.

6. To install the replacement display, follow the above steps in the reverse order.
The replacement display comes with a protective foil. Therefore, the following
sequence of steps is recommended:
a) Attach the replacement display to the front panel.
b) Connect all cables.
c) Peel off the protective foil from the display window. Verify that the display window is
clean. If it is not, clean the window.
d) Reattach the outside cover plate to the front panel door.
7. Turn on the pump and check whether the screens on the front panel display are clearly
legible. If they are not, adapt the brightness and/or contrast (→ page 38).

6.20.2 Replacing the Front Panel LED Board

Front panel LED board 6820.0220
1. Remove the outside cover plate from the front panel door (→ page 113).
2. Tilt the front panel door upward.
3. Remove the two screws that attach the LED board to the front panel door.
Ferrite LED board cable

toroidal core connected to
display board
Front panel
LED board
Fig. 87: Removing the front panel LED board
4. Remove the cable from the cable slots.

5. Unplug the LED board cable from the connector on the display board.
6. To install the replacement board, follow the above steps in the reverse order.
The ferrite toroidal core is secured with a cable tie on the replacement board cable.
Remove the cable tie and push the core to the appropriate position as indicated in the
picture.
Secure the cable again in the cable slots.

6.21 Vacuum Degasser (LPG-3400)

In normal operation, the degasser is quiet. Even if the vacuum pump is running, the
operating noise is very low. Higher frequencies may slightly increase the noise level, but
this does not impair the degassing performance.
To ensure optimum degassing performance, observe the general notes for degasser operation
in the Operating Instructions for the pump.
6.21.1 Rinsing the Degassing Channels
To avoid contamination of the degasser, prepare fresh solvents, clean the solvent supply
lines, and rinse the degassing channels at regular intervals.
Rinsing the degassing channels is especially important for the channel that degasses aqueous
solvents (for reverse-phase chromatography).
Usually, rinsing all channels with organic solvent is sufficient. (Use a fresh bottle.) When you
use water and acetonitrile or methanol, to rinsing the degassing channels once per week is
sufficient.
However, adapt the rinsing intervals to the solvents in use. In persistent cases, for example,
reproducible ghost peaks in the chromatogram, follow the steps below:
1. Install a backpressure capillary on the pump outlet. The capillary should be

appropriate for generating a backpressure of 20 to 30 MPa.
2. Rinse the degassing channels for 1 hour with about 20% nitric acid at the flow rate
normally used for the application. Use fresh HPLC-grade water.
3. Rinse the degassing channels with fresh HPLC-grade water until the pH value is
neutral.
4. Rinse the degassing channels for two hours with fresh acetonitrile at the flow rate
normally used for the application.
5. Prepare fresh solvents for the application (using new bottles) and connect them to the
degassing channels.
6. Install new filter frits on the solvent supply lines.
7. Uninstall the backpressure capillary and reconnect the system as required by your
application.
8. Equilibrate the system as described in the Operating Instructions for the pump.

6.21.2 Replacing a Degassing Chamber

Degassing chamber (analytical) 6030.9289
Note: The optical appearance of the replacement chambers may be different from those
installed in the pump. However, this does not influence the replacement procedure.
1. On the interior front panel, disconnect the solvent supply lines from the degassing
chambers that you want to replace.
3. On the rear of the degassing chambers, remove the tubing from the chambers that you
want to replace.
Fig. 88: Degassing chambers (view from the rear)
4. Remove the screws that attach the degassing chambers to the interior front panel and
remove the degassing chamber.
Screws attaching the

degassing chambers
Fig. 89: Degassing chambers (view from the inside front panel)
5. To install the replacement degassing chamber, follow the above steps in the reverse
order.
6. To remove any residues that may have collected in the vacuum pump due to a
defective degassing chamber, rinse the vacuum pump before resuming operation of the
degasser (→ page 120).

6.21.3 Degasser Vacuum Pump
6.21.3.1 Replacing the Degasser Vacuum Pump

Degasser vacuum pump 6040.1981
Note:
The replacement degasser vacuum pump looks different from the vacuum pump with which the pump is
shipped from the factory. Also, the vacuum pumps are connected to different connectors on the main
board. For details, see section 4.1 Main Board (→ page 14).

2. Unplug the degasser vacuum pump cables from the connectors on the main board
(→ page 14).
3. Remove the following:
• On the degasser cartridges, remove the vacuum tubing that connects the cartridges to
the degasser vacuum pump.
• On the vacuum pump, remove the exhaust line.
• Remove the screws that attach the degasser vacuum pump to the enclosure bottom.
There are four screws.
Remove exhaust line (bottom
connector on vacuum pump)
Remove the tubing on this

cartridge (top connector)
Remove the tubing on this

cartridge (bottom connector)
Remove 4 screws
Fig. 90: Degasser pump tubing (here vacuum pump installed upon pump shipment)

4. Remove the degasser vacuum pump.

5. Attach the replacement vacuum pump to the enclosure bottom with four screws.
Tighten these screws Tighten these screws.
Fig. 91: Screws attaching the replacement degasser vacuum pump in the enclosure
6. Connect the vacuum tubing and exhaust line (→ Fig. 92).

• Connect the vacuum tubing that connects the degasser vacuum pump to the degasser
cartridges.
To prepare the replacement vacuum tubing, use a tubing cutter to cut the silicone
tubing (included in the shipment) to the appropriate length (same length as the tubing
you removed). Make sure that the cut is at a right angle to the length of the tubing.
• Connect the exhaust line.
Connect vacuum
tubing here.
Connect vacuum tubing here

(vacuum pump and bottom
connector on cartridge)
Connect the exhaust line

here.
Fig. 93: Degasser tubing
7. Connect the degasser vacuum pump cables to the connectors on the main board
(→ page 14).
8. Turn on the pump and check the degasser vacuum pump for correct operation.

6.21.3.2 Rinsing the Degasser Vacuum Pump

Residues may have collected in the vacuum pump due to a defective degassing chamber.
Therefore, rinse the vacuum pump to remove the residues after you have replaced one or
more degassing chambers.
Note: A syringe with a volume of 50 mL or more is required for the procedure.

2. On the degassing chambers, remove the tubing that connects the chambers to the
vacuum pump. (The tubing remains connected to the vacuum pump.)
Remove tubing here
Exhaust line
Fig. 94: Degasser tubing
3. Remove the exhaust line from the tubing clip on the enclosure bottom. (The tubing
remains connected on the vacuum pump.)
4. Place the free end of the exhaust line into a beaker.
5. Draw about 50 mL of water into a syringe, and then attach the free end of the vacuum
tubing to the syringe.
6. Push the plunger to direct the water through the vacuum pump and into the beaker.
7. Remove the syringe from the vacuum tubing.
8. Remove any water that might be left in the syringe and empty the beaker.
9. Draw air into the syringe and reattach the free end of the vacuum tubing to the syringe.
10. Push the plunger to direct air through the pump. Continue until no more water leaves
the exhaust line.
11. Turn on and run the degasser, without reconnecting the tubing, for about 10 minutes.
When a message appears, interrupting operation, turn the degasser off and on again to
continue.
12. Reconnect the tubing.

6.22 Pulse Damper
6.22.1 Replacing the Pulse Damper

Pulse damper for pumps with serial numbers:
Up to 8086057 6042.6000
8086058 and higher 6042.6010
Capillary from purge unit to pulse damper Included in 6042.3002
Capillary from pulse damper to inline filter Included in 6042.3002
1. Before replacing the pulse damper, allow the pressure to drop below 1 MPa before
turning off and disconnecting the pump from its source.
2. Disconnect the capillaries on the inlet and outlet of the pulse damper. You can remove
the pulse damper module more easily if you disconnect the pulse damper inlet
capillary also on the purge unit and remove the inline filter from the mounting bracket.
Pulse damper
outlet capillary
Pulse damper
inlet capillary Pulse damper screws
Fig. 95: Pulse damper

3. Remove the four screws that attach the pulse damper module to the interior front panel
and pull the module toward you and out of the pump.
4. Four screws attach the pulse damper in the mounting module. Turn the module,
remove the four screws, and remove the pulse damper from the module.
Fig. 96: Pulse damper module (rear view)

(position of screws depending on pulse damper)
5. To install the replacement pulse damper, follow the above steps in the reverse order.

6.22.2 Replacing the Pulse Damper Membrane

Pulse damper maintenance kit for pumps with serial numbers:
Up to 8086057 6042.6001
8086058 and higher 6042.6011
The kit includes all components required for pulse damper maintenance.
In addition, you will need 40 mL of HPLC or reagent grade isopropanol,
a vise to hold the pulse damper during the maintenance procedure, a
syringe, and a torque wrench (optional).
To replace the pulse damper membrane, follow the instructions that are included in the kit.

6.23 Shutting Down the Pump

Before interrupting the operation or before shipping the pump, observe the following
precautions
• Rinse out any solvents if necessary. Fill the pump with methanol or a similar alcohol,
such as 2-propanol or ethanol. If a buffer is used as a part of the mobile phase, flush the
system with several volumes of methanol/water (50:50) before it is shut down. This
prevents salt buildup inside the unit.
• If you interrupt operation for more than 5 days, remove the tubing from the peristaltic
pump. Slightly press the lever to the left, remove the tubing, and release the lever. This
prevents that the tubing remains compressed and does not relax, thus blocking the wash
solution.
• If you interrupt operation for more than one week, fill the pump with methanol or a
similar alcohol, such as 2-propanol or ethanol. If the solvents in the pump are not
miscible with water, use an appropriate intermediate solvent.
• Rinse out buffers or solvents that form peroxide.
• To prevent evaporation of the solvents in the flow cell, turn off the lamps in any UV or
RF detectors that are connected to the pump.
• When using an electrochemical (EC) detector with the pump, always turn off the
potential to the EC cells before shutting off the pump flow to prevent damage to the cells.
• If you want to ship or move the pump to a new location, no liquid must remain in the
waste line of the rear seal wash system. (The waste line is routed under the pump to the
drain port of the pump.) If necessary, lift and tilt the pump from the left side of the
enclosure and wait until no more liquid leaves the waste line.
• Ship the pump only in the original shipping container and observe the packing
instructions. If the original shipping container is not available, you can order appropriate
shipping containers and packing material from the Thermo Fisher Scientific sales
organization. The packing instructions are included in the "Installation and Qualification
Documents for Chromatography Instruments" binder; they are available also on request.
Shipping the unit in any other packaging automatically voids the warranty.
From Chromeleon, you can set the pump and HPLC system into the standby mode or
automate system shutdown. For details, see the Chromeleon Help.

7 Troubleshooting
7.1 Overview
The following features help you to identify and eliminate the source for problems that may
occur during the operation of the pump.
• Status Indicators
♦ The status indicators on the front panel display provide a quick visual check of the
operational status of the pump. They indicate whether the pump is turned on,
connected in Chromeleon, and operating properly.
♦ The pump block status indicator on the interior front panel of the pump provides a
quick visual check of the operational status of the pump block (→ page 125).
• Messages
If a fault or error is detected during the operation of the pump, a message appears on the
pump display. Check the Messages on the Pump Display section for recommended
courses of action (→ page 126). If the pump is operated from Chromeleon, the message
is displayed also in the Chromeleon Audit Trail.
Tip: For information about operating problems that might occur during the
operation of an UltiMate 3000 system, see page 140.
• Diagnostics Tests
If the pump is operated from Chromeleon, Chromeleon provides several diagnostic tests
allowing you to check the performance of certain pump components. If a test fails, check
the Chromeleon Diagnostics Messages section for a short description of possible causes
along with recommended courses of action (→ page137).
Tip: To test the pump for leakage when the pump is not connected in
Chromeleon, see page 110.
If you are unable to eliminate a problem following the instructions given here, contact your
local Thermo Fisher Scientific support center (→ page 151).
Tip: If the UltiMate 3000 system is operated from Chromeleon, Operational and
Performance Qualification allow you to check and document the performance
of the system. All required materials and detailed instructions are available in
the Performance Qualification kit.

7.2 Pump Block Status LED

The pump block status LED provides a quick visual check of the operational status of the
pump block:
LED Operational Status
The LED is dark. No flow

The LED is green. The pump head is delivering.
The LED flashes green:
In normal operation In addition, the message 'Compression limit reached' appears in the
Chromeleon Audit Trail.
During each of past three strokes, the compression reached 100%.
When the compression later remains lower than 100 % for several
strokes, the message 'Compression back to normal' appears in the Audit
Trail.
For more information about the compression value, see section 7.6
(→ page 148).
During pump maintenance The pistons are in the appropriate position for piston (seal) replacement.
The LED flashes red. The pistons are not in the appropriate position for normal operation (for
example, after piston (seal) replacement) or an error occurred during
internal maintenance (→ page 28).
In both cases, the following message appears on the pump display
"Undock err., open purge valve". (A similar message appears in the
Chromeleon Audit Trail.) To resolve the problem:
1. Open the purge valve.
With an ISO-3100BM, observe the precautions on page 7.
2. Perform the Dock Pistons command.
3. Close the purge valve.

7.3 Messages on the Pump Display

Each time a fault or error occurs during the operation of the pump, the Status LED on the
front panel changes to red and a message appears on the front panel display. In this case,
the Prev, Next, and Clear keys appear on the navigation bar.
To … Select …
Return to the previous message. Prev

Proceed to the next message. Next
Remove all messages from the display. Clear
These keys are active also when the pump is connected in Chromeleon.
When the pump is operated from Chromeleon

• The message is displayed also in the Chromeleon Audit Trail.
The Audit Trail may provide additional information.
• To remove a message from the front panel display you can perform also the
ClearDisplayError command in Chromeleon.
The table below lists pump-related messages and suggests appropriate remedial actions. The
component for which the message appears may be indicated in front of the actual message
text. If you are unable to eliminate a problem following the instructions given here, contact
your local Thermo Fisher Scientific support center (→ page 151).
In addition to the messages in the table, other messages may appear. If you are unable to
eliminate the problem, note the exact wording of the message. Contact your local Thermo
Fisher Scientific support center.
Message Remedial Action
(x) counts deviation in zero (where x is the exact deviation from the zero position.)
position Turn the pump off and on again by pressing the power switch on the
rear of the pump.
If the message appears more often, verify that the Hall sensor cable is
properly connected on the pump block board (Fig. 5, page 16) and/or
consider replacing the pump block (→ page 103).
All flows need to be stopped You tried to undock the pistons for piston or piston seal maintenance
before servicing the pistons. although the flow rate was not 0.
Set the pump flow rate to 0, wait until the system pressure is down to
zero, and perform the command again.
An emergency stop was This message appears when a Corona or Coulochem III detector is
requested over the digital connected to the pump and the digital input has been configured for
input line remote emergency stop if an error or fault occurs for the detector.
Check the detector and take appropriate remedial action (→ Detector
manual).

Can't do this when the flow is You tried to perform a self test or to perform the SetPressureZero
on. command for calibration of the pressure transducer while the pump
flow was not yet 0.
Set the pump flow rate to 0 and perform the command again.
Can't reset pressure sensor. The pump pressure is not down to zero during the self test. Wait until
Pressure is not constant. the pressure is down and repeat the test. Open and close the purge
valve if necessary to reduce the pressure.
Refer to section 7.7 for additional checks and remedial actions
(→ page 149).
Can't reset pressure sensor. The pump pressure is not down to zero during the self test. Wait until
Pressure is out of range. the pressure is down and repeat the test. Open and close the purge
Refer to section 7.7 for additional checks and remedial actions
(→ page 149).
Can't set serial number. An attempt was made to the set the serial number for a pump for
which the serial number has already been set. (Note that it is not
possible to change the serial number.)
Can't start pump while alarm An alarm has occurred, for example, a leak alarm. You can restart the
is on. pump flow only after having acknowledged the alarm, for example,
by performing the AlarmOff command in Chromeleon.
Configuration error: Found x (where x and y are numbers)
ID chips and y pump blocks. A pump block may be defective. If the pump has two blocks,
disconnect either block from the main board to find out which block
is defective. Replace the pump block (→ page 103).
Connection error: Main The message appears if not all pump blocks are connected to the main
board to pump block board.
Verify that the cables between the main board and pump blocks are
properly connected. Replace the cables if necessary.
Connection error: Reconnect Verify that the control cables between the pump blocks and main
pump block to main board. board is properly connected.
The cables may be defective. Replace the cables.
Degasser malfunction LPG-3400
The vacuum level monitoring function of the degasser in the pump
recognized insufficient vacuum. Possible causes are:
• The degasser status cable and/or power cable is not connected
properly or is defective.
• The degassing module is leaking.
Turn the pump off and on again by pressing the power switch on the
rear of the pump. Check the degasser vacuum in Chromeleon
(Commands dialog box). After about 1 minute, the setting should
change from NotOk to OK. If the message appears again, check the
degasser status and power cables for proper connection. Else, the
degassing module may be defective. Replace the degassing chambers
(→ page 117).

Degasser malfunction Pumps to which a SRD-3x00 Solvent Rack is connected

(Cont'd) The vacuum level monitoring function of the degasser in the
SRD-3x00 Solvent Rack recognized insufficient vacuum.
Verify that the Solvent Rack and the pump are properly connected
and that the degasser is turned on.
Turn the Solvent Rack off and on again by pressing the standby
button on the front panel. If the message appears again, the degassing
module may be defective (→ Service Instructions for the Solvent
Rack).
I2C device xx does not work (where xx indicates the pump block: 66 = right block, 64 = left block)
rear of the pump.
Verify that the control cable for the pump block is properly plugged
in on the pump block board and on the main board.
The pump block may be defective. Replace the pump block
(→ page 103).
Incorrect hardware The message appears, for example, if you installed a pump block or
configuration. You may main board that was not a replacement part or that had been
enable operation for 48 associated permanently to another pump before.
hours. The pump will run for 48 hours and then stop operation
automatically.
Replace this component with a replacement component (→ page 103
for the pump block and/or page 100 for the main board).
Internal timing error Turn the pump off and on again by pressing the power switch on the
rear of the pump.
If the message appears again, update the pump firmware.
Internal pump maintenance is Internal pump maintenance is overdue for more than 45 days. Open
due, please open purge screw the purge screw and perform a purge cycle.
and purge
Leak detected When this message appears, the flow is automatically turned off after
180 seconds
The leak sensor has reported a leak.
Find and eliminate the source for the leak. Dry the leak sensor and
tray.
Motor current too large. The motor current is too high when the pump is running.
The flow path before the system pressure sensor may be blocked.
Make sure that neither the capillary from the working cylinder to the
equilibration cylinder nor the capillary to the purge unit is blocked.
Replace the components if necessary.
Verify that the check valve cartridges are installed in the direction of
flow (→ page 49).
Inspect the purge valve for indications of blockage. Replace the
purge valve knob if necessary (→ page 47).
Verify that the pressure transducer cables are properly connected to
the connectors on the interior front panel. Reconnect the cables if
necessary.
The motor is defective. Replace the pump block (→ page 103).

Motor controller missed a Turn the pump off and on again by pressing the power switch on the
breakpoint rear of the pump.
If the message appears again, update the pump firmware.
New hardware configuration This message appears if the main board or a pump block has been
detected. After 48h replaced. The pump-specific data, such as, the calibration values, will
operation, spare parts will be be synchronized with the data on the pump block or main board,
associated permanently with respectively. Afterward, you can test the pump for 48 hours.
this pump. • If the test operation is not satisfactory, remove the replacement part
within 48 hours.
• If the test operation is satisfactory, no further action is required.
After 48 hours, the data will be saved permanently to the replacement
part.
No abortable command was An attempt was made to perform an Abort command. However, no
executing. action or command was being executed that can be aborted.
Please cycle power and This message appears only for pumps with two pump blocks if one or
disconnect the left pump both pump block and the main board have been replaced at the same
block from the main board. time.
In both cases, turn off the power to the pump, disconnect the control
cable for the left pump block from the main board, and turn on the
pump.
Position error Turn the pump off and on again by pressing the power switch on the
rear of the pump. If the message appears again, replace the pump
block (→ page 103).
Pressure buildup during A pressure of more than 0.5 MPa built up during piston and/or piston
maintenance operation. seal maintenance. Open the purge valve (with an ISO-3100BM,
Please open the purge valve. observing the precautions on page 7) to reduce the pressure.
If the message appears during internal maintenance (→ page 28),
initiate another purge cycle (→ Operating Instructions for the pump).
Pressure fallen below lower The solvent reservoirs are empty. Fill the reservoirs and purge the
limit system (→ Operating Instructions for the pump).
There are air bubbles in solvent supply line. Check the filter frits;
purge the system (→ Operating Instructions for the pump).
The solvent emits gas when mixing. Degas the solvent. Check the
degasser.
There is a leak in the system. Find and eliminate the source for the
leak. Tighten loose connections.
One of the check valves is defective. Clean or replace the valve
(→ page 49). Purge the system (→ Operating Instructions for the
pump).
The lower pressure limit cannot be reached for the specified flow.
The main task of the lower pressure limit is to monitor the system for
leakage. Leakage may occur especially at the fittings and or screw
joints, the switching valve of the autosampler, or the piston seals.

Pressure recalibration The message appears when the pressure value that is reported during
deviates by xx bar the self test or when the SetPressureZero commands is performed
deviates by more than 0.2MPa from the former value.
If the message appears after you have replaced the purge unit or a
pump block, no action is required. The self test is passed and the new
offset value is stored.
All other cases: The pump pressure has not been down to zero during
the self test or execution of the SetPressureZero command. Wait until
the pressure is down and repeat the test or command. Verify that the
cable for the system pressure transducer is properly connected to the
P-Sys connector on the interior front panel. Open and close the purge
Pressure sensor malfunction The pressure transducer for the system pressure reported a pressure
under 0 MPa.
Refer to section 7.7 for checks and remedial actions (→ page 149).
Pump drive flow limit The message appears for an HPG-3200BX if the selected flow rate is
exceeded. above the permissible range. Check and change the flow rate setting
and/or solvent composition as required, observing the information for
the double flow mode (→ Operating Instructions).
Purge pressure limit After you have initiated purging in Chromeleon (Purge property set
exceeded. to On), pressure of more than 5 MPa built up.
Verify that the purge valve is open. If it is not yet open, open the
valve (with an ISO-3100BM, observing the precautions on page 7).
Check the flow path for indications of blockage (also, see "Upper
pressure limit exceeded").
Quiescent current too large. The motor current is too high when the pump is stopped.
rear of the instrument.
If the message appears again, the switching power supply may be
defective, or in rare cases, the main board or pump block may be
defective.
Replace the power supply (→ page 108). If the message appears
again, replace the main board (→ page 100). If this message persists,
replace the pump block (→ page 103).
Self test failed: xx (where xx = additional text)
This message usually appears together with another message
indicating which failure occurred during the self test. Refer to this
message for recommended remedial actions.
Serial number is not set. The message may appear when the main board and all pump blocks
have been replaced. To enter the serial number, open the Commands
dialog box in Chromeleon. Under PumpModule_Service, select
SetSerialNo and type the serial number from the type label on the
rear panel of the pump into the input field.
Solvent rack leak detected. The leak sensor in the Solvent Rack has reported a leak.
The Solvent Rack may not be connected properly to the pump. Verify
that the Solvent Rack and the pump are properly connected.

Solvent rack leak detected. There is a leak in the system or a fluid connection is loose. Find and
(Cont'd) eliminate the source for the leak. Tighten leaking connections and dry
the leak sensor (→ Solvent Rack manual).
The cam position is yet The position of the cam is unknown after the piston has been
unknown. It is necessary to replaced. Turn on the flow and have the pump run for two revolutions
run at least 2 revolutions. to establish the position.
The piston seal leakage has Inspect the piston seals for leakage (→ page 57) and replace them if
exceeded the recommended necessary (→ page 65).
limit. The peristaltic pump lever or the peristaltic pump may be defective.
• Stop the pump flow and the peristaltic pump. Remove the tubing
from the seal wash detector and check whether wash solution exits
the free end of the tubing while the peristaltic pump is off.
• Place the seal wash reservoir at a lower position to reduce the
hydrostatic pressure.
• Verify that the lever of the peristaltic pump is working correctly.
• Replace the peristaltic pump (→ page 80).
Consider also checking the value reported by the RearSealLeak
Counter (→ page 23).
The pressure exceeded the The message appears when pressure builds up extremely fast. This
absolute limit. may happen, for example
- When the pump delivers at a high flow rate while the pump outlet is
closed with a bind nut.
- If a pump with a low-volume mixing system (mixing volume
≤ 200 µL) delivers at a high volume rate and high pressure, and the
valve in the autosampler switches from Load to Inject or vice versa.
Take appropriate remedial action and retry.
Monitor the pressure with a calibrated pressure gauge. Connect the
pressure gauge to the pump, using a short capillary to ensure equal
pressures on both sensors. If the pressure reading on the pump display
and on the pressure gauge differs by more than 1MPa, manually
calibrate the pressure transducer for the system pressure, following
the steps in the "If you cannot use the value from the label" section
(→ page 37). If the message appears again, refer to section 7.7 for
additional checks and remedial actions (→ page 149).

The pressure signals are not The error can be due to large amounts of air in the pump heads, for
consistent. Air may be example, during initial installation.
trapped in the working Verify that the pressure transducer cable of the pump head is properly
cylinder. connected to the P-Work connector on the interior front panel.
Purge the pump heads thoroughly (→ Operating Instructions for the
pump). Perform a self test if necessary.
It depends on the firmware version of the pump whether the message
text includes the pump block for which the message appears
(firmware version ≤ 2.40). If it does not, check the Audit Trail
message for the block information. If the bock information is not
available, identify the pump block as follows: Have one pump block
deliver 100%. If the message does not appear for this block, have the
other block deliver 100% to verify that the message appears for this
block.
Perform the following sequence of actions for the block for which the
message appears (here for the left block):
1. Remove the left pump head (→ page 60) and set it aside.
2. Remove the right pump head and install it on the left block
(→ page 62).
3. Install the left pump head (which was removed in step 1) on the
right block.
4. Have the left block deliver 100%.
5. Depends on whether the message appears again
A) If it does, the electronics of the left block may be defective.
Reinstall the pump heads to their original positions and refer to
page 150 for additional remedial actions.
B) If it does not, proceed with the next step.
6. Have the right block deliver 100%.
7. Depends on whether the message now appears for the right block
A) If it does not, reinstall the pump heads to their original
positions and refer to section 7.7 for additional checks and
remedial actions (→ page 149).
B) If it does, the pump head is defective. In this case, perform the
following sequence of actions:
a) Remove the pump head from the right block and reinstall
the pump head that was originally installed in this position.
b) Install a replacement pump head on the left block.
c) Have both blocks deliver 100% to check whether the
message appears again.
d) If it does, calibrate the pressure transducer for the system
pressure (→ page 37).
e) Have both pump blocks deliver 100% .
f) If the message appears again, refer to page 150 for
additional remedial actions.

The pump drive is still in You tried to start the pump while the pump was still in maintenance
maintenance position. position, for example, during piston replacement or piston seal
replacement. Return the pistons into the position for normal operation
(→ page 63) and retry.
If the message appears during internal maintenance (→ page 28),
initiate another purge cycle (→ Operating Instructions for the pump).
The rear seal leak sensor Consider checking the value reported by the RearSealLeakCounter
detects drops constantly. (→ page 23). The value provides helpful information for trouble-
shooting.
Remove the detector of the seal wash system. Verify that the detector
seat is dry, for example, by using a lint-free cloth or tissue.
Verify that the pump is installed in a horizontal position and that the
seal wash solution can run properly to the waste.
The message appears also if the seal wash system is fitted with the
NP detector and seal wash solution with high water content is used.
Observe the information about the composition of the seal wash
solution (→ Operating Instructions for the pump).
Consider installing the RP detector (→ page 77) and observe the
information about the composition of the seal wash solution.
Inspect the electrodes in the seal wash detector for indications of
contamination. Clean the electrodes if necessary (→ page 78).
If the message continues to appear after the above actions have been
taken, the detector of the seal was system might be defective.
Consider replacing the detector (→ page 77).
The peristaltic pump lever or the peristaltic pump may be defective.
• Stop the pump flow and the peristaltic pump. Remove the tubing
from the seal wash detector and check whether wash solution exits
the free end of the tubing while the peristaltic pump is off.
• Place the seal wash reservoir at a lower position to reduce the
hydrostatic pressure.
• Verify that the lever of the peristaltic pump is working correctly.
• Replace the peristaltic pump (→ page 80).
The rear seal wash system Consider checking the value reported by the RearSealLeakCounter
has run out of wash solution. (→ page 23). The value provides helpful information for trouble-
shooting.
Rear seal washing is enabled and the peristaltic pump is running, but
no liquid reaches the detector of the seal wash system.
Make sure the wash solution is present in the seal wash reservoir.
Make sure that the seal wash solution is sufficiently conductive and
that the wash solution is appropriate for the installed detector type
(RP or NP) (→ Operating Instructions for the pump).
Initiate another wash cycle manually. To do so, set
RearSealWashPump to Active (in Chromeleon in the Commands
dialog box for the pump).

The rear seal wash system Verify that the seal wash tubing is connected properly and check the
has run out of wash solution. tubing for permeability. Replace the tubing and tubing connectors as
(Cont'd) necessary.
The peristaltic tubing may be blocked or draws air. Replace the
peristaltic tubing as necessary (→ page 79).
Inspect the rear seal wash system for indications of leakage
(→ page 77 ). Replace the seal wash tubing and the tubing connectors
if necessary.
Verify that the peristaltic pump is operating properly and that the
lever (→ Fig. 53, page 79) is not blocked. Replace the peristaltic
pump (→ page 80).
Inspect the electrodes in the seal wash detector for indications of
contamination. Clean the electrodes if necessary (→ page 78).
Undock err., open purge (Usually, also the pump block Status LED flashes red.)
valve The pistons are not properly docked or an error occurred during
internal maintenance (→ page 28). Open the purge valve (with an
ISO-3100BM, observing the precautions on page 7), perform the
Dock Pistons command, and close the purge valve.
Unexpected hardware The message appears, for example, if you installed a pump block or
configuration. Test operation main board that was not a replacement part or that had been
was limited to 48 hours. associated permanently to another pump before. In this case, the
pump stops operation after 48 hours.
Replace this component with a replacement component (→ page 75
for the pump block and/or page 100 for the main board).
Upper pressure limit Check whether the flow path behind the purge unit is blocked. To do
exceeded so, open the purge valve (with an ISO-3100BM, observing the
precautions on page 7).
If the flow path is blocked:
- The column may be contaminated. Rinse or replace the column. If
the problem occurs due to column ageing, it may be sufficient to
increase the setting for the upper pressure limit.
- The autosampler may be blocked. Find and eliminate the source for
the blockage.
If the flow path is not blocked:
Depending on the pump type, check the static mixer or inline filter for
indications of blockage (→ page 87 or 96).
Working piston zero pressure Refer to section 7.7 for checks and remedial actions (→ page 149).
out of range
Zero position not found Turn the pump off and on again by pressing the power switch on the
during the last revolution. rear of the pump.
If the message appears more often, verify that the Hall sensor cable is
properly connected on the pump block board (Fig. 5, page 16). If it is,

Zero position not found Turn the pump off and on again by pressing the power switch on the
during the last 3 revolutions. rear of the pump.
If the message appears again, verify that the Hall sensor cable is
properly connected on the pump block board (Fig. 5, page 16). If it is,

If communication between Chromeleon and the pump cannot be established, related

messages may appear in the Chromeleon Audit Trail.
HPG-3400USB-1610103 - The USB connection between the pump and the Chromeleon server
Device not found on the USB may be interrupted. Check the USB connection.
The power supply to the pump may be interrupted. Check the power
supply connection of the pump.
Restart the Chromeleon computer and/or Chromeleon server.
Error opening HPG-3400 The USB connection between the pump and the Chromeleon server
@USB-1610103 – The System may be interrupted. Check the USB connection.
cannot find the file specified The power supply to the pump may be interrupted. Check the power
Error issuing control request to The USB connection between the pump and the Chromeleon server
HPG-3400@USB-1610103 may be interrupted. Check the USB connection. Check the power
Remove the pump specified in the message from the server
configuration or else, select a different pump from the list of available
pumps in the server configuration program.
Error reading from HPG-3400 Check the USB connection. The connection to the next hub must not
@USB-1610103 exceed 5 m. The overall connection length, including the hub
Data error (cyclic redundancy connections must not exceed 30 m.
check) Replace any defective USB cable or hub.
Error reading from HPG-3400 The USB connection between the pump and the Chromeleon server
@USB-1610103 may be interrupted. Check the USB connection.
The power supply to the pump may be interrupted. Check the power
Tip: Overload of the USB interface of the Chromeleon server may also result in
communication problems. Try the following:
Right-click My Computer and click Properties on the menu. On the
Hardware tab, click Device Manager. Click the Plus sign next to Universal
Serial Bus controllers. Double-click the first USB Root Hub on the list. On
the Power Management tab, clear the Allow the computer to turn off this
device to save power check box. Repeat this step for all other USB root hubs
on the list.
If this does not eliminate the problem, the computer hardware may be
defective.

7.4 Chromeleon Diagnostics Messages

If the pump fails a diagnostics test, perform the instructions given here and repeat the test.
If the test fails again, contact your local Thermo Fisher Scientific support center
(→ page 151).
Degasser Vacuum Test failed
Probable Cause Remedial Action
The degasser module did not reach Check the tubing of the vacuum system and the degasser module.
the appropriate operating pressure.
Repeat the test. If the test fails again, the degasser module may
be defective. Replace the degassing chambers and/or the degasser
vacuum pump.
LPG-3400
For details how to the replace the
- degassing chambers, see page 117.
- degasser vacuum pump, see page 118.
Pumps to which a SRD-3x00 Solvent Rack is connected
→ Service Instructions for the Solvent Rack
Mixer Frit Test failed
The backpressure has exceeded the All pumps except LPG-3400BM and DGP-3600BM
limit. The static mixer or inline filter Depending on the pump type, replace the static mixer
may be blocked. (→ page 85) or inline filter (→ page 92).
LPG-3400BM and DGP-3600 BM
Replace the inline filter (→ page 94) or the frit in the inline filter
(→ page 95).
General Leak Test failed
The pressure drop has been too high.

Check the blind nut on the pump outlet for tightness.
The pressure level has not been
Visually inspect all fluidic connections for liquid droplets.
reached in time.
Check all fluidic connections for tightness.
The initial pressure has not been Consider replacing the valve cartridge (inlet check valve)
reached within the first minute.
(→ page 49).
The pressure drop during relaxation If you still suspect there is a leak, run the detailed leak test.
has been too high. The pump has a
leak.

Detailed Leak Test failed
The check valve has a leak. Replace the valve cartridge (outlet check valve) (→ page 49) and
repeat the test.
Replace the valve cartridge (inlet check valve) (→ page 49) and
repeat the test.
The piston seals are leaking. Replace the piston seals (→ page 65).
Performance Test failed
Test Result Probable Cause Remedial Action
The pressure/% compression Eluent may not have been Degas the eluent.
was outside specification! degassed correctly.
The wrong eluent may have been Repeat the test. Make sure that the
chosen in test step 1 (Prepare correct eluent is chosen.
Pump).
There may be air bubbles in the Purge the pump as described in the
pump head. Operating Instructions for the
pump.
The check valves may not be Consider replacing the check valve
working correctly. cartridges (→ page 49).
Seals may not be tight. Consider replacing the piston seals
(→ page 65).
Backpressure may have Consider replacing the restrictor.
fluctuated due to problems with
the restrictor. The restrictor may
be blocked.
The pressure was outside The pressure was outside the Change the flow settings and repeat
specification. upper and lower pressure limits the test.
(that is, p < 5 MPa and
p > 40 MPa).
The % compression value was There may be air bubbles in the Purge the pump as described in the
over the limit! pump head. Operating Instructions for the
pump.
The flow may have been too Repeat the test with reduced flow.
high.
The % compression value was The flow may have been too low. Repeat the test with increased flow.
below the limit!
Pressure ripple was too high. There may be a mechanical fault. Repeat the test.

Test Result Probable Cause Remedial Action
The pulse damper may not The pulse damper may not Refill the pulse damper; follow the
work properly. contain sufficient liquid. appropriate steps in section 6.22.2
(→ page 122).
The pulse damper membrane Replace the membrane
may be ruptured. (→ page 122).
The pulse damper may be Replace the pulse damper
defective. (→ page 121).

7.5 Operating Problems

The table below provides information about common operating problems that might occur
with the pump or an UltiMate 3000 system and lists probable causes, as well as remedial
actions.
For additional information and remedial actions, refer to the manuals for the other modules of
an UltiMate 3000 system.
Problem Probable Cause Remedial Action
A continuous beep sounds. There is firmware problem. Turn the power to the pump
off and on again.
Three beeps sound when the The pump firmware may be Update the firmware
pump is turned on. defective or is not installed (→ page 153).
properly.
A beep sounds and the pump Contact your local Thermo
can be operated neither from Fisher Scientific support
the display nor from center (→ page 151).
Chromeleon.
No information appears on The pump is not connected to the Connect the power cord.
the pump display. mains.
The power is turned off. Turn on the power to the
pump.
The pump is in standby mode. Press the Standby key on the
front panel.
The screen brightness or contrast Adjust the brightness and/or
is not adjusted correctly. contrast (→ page 38).
The fuses blow. Replace the fuses
(→ page 44).
Replacement fuse blows Contact your local Thermo
immediately. Fisher Scientific support
center(→ page 151).
An error occurred in the Contact your local Thermo
electronic system. Fisher Scientific support
center (→ page 151).
The pump does not work There is no connection between Check the USB cable and
correctly when operated from the pump and the Chromeleon connection to the computer.
Chromeleon. computer.
The USB port on the computer is Check the USB port on the
not ready for operation. computer.
The rear seal wash system is The seal wash tubing is not Check the seal wash tubing.
leaking. connected properly; the tubing is Be sure that it is properly
damaged by bending or is connected and neither
blocked. blocked nor kinked. Replace
the tubing as necessary.

The degasser of a SRD-3x00 The degasser is not configured in In the Chromeleon Server
Solvent Rack that is the properties dialog for the Configuration program,
connected to the pump cannot pump. verify on the Devices page of
be turned on via the pump. the properties dialog for the
pump that Degasser Type is
set to External.
The degasser of a SRD-3x00 The Solvent Rack is not Verify both that the Solvent
Solvent Rack that is connected properly with the Rack is connected properly to
connected to the pump does pump and/or the degasser is not the pump and that the
not work (the Vacuum and configured in the properties Degasser Type is set to
Status LED on the Solvent dialog for the pump. External in the properties
Rack are off). dialog for the pump.
The degasser is turned off. Turn on the degasser.
A first generation SRD-3x00 Check the serial number of
Solvent Rack is connected to the the Solvent Rack. First
pump. generation Solvent Racks
have serial numbers ≤
8003272. If the Solvent Rack
is first generation, you have to
update the adapter board in
the Solvent Rack. To do so,
order part no. 6030.0004 and
follow the instructions that
are shipped with the board.
The system has very high One or more capillaries in the Check the capillaries in the
backpressure. system are blocked or damaged system systematically from
by bending. the detector to the pump.
Replace the capillaries as
needed.
Depending on the pump type: Replace the static mixer
The static mixer or inline filter is (→ page 85) or inline filter
blocked. (→ page 92 or 94).
With a LPG-3400BM and
DGP-3600BM, you can
replace the filter frit in the
inline filter (→ page 95).
The column is contaminated or Rinse or replace the column.
blocked.
For additional causes, refer to the → TCC manual
service instructions for the
column compartment.
High baseline drift The column is contaminated. Clean or replace the column.
The system is not sufficiently Flush the system until
equilibrated. equilibration. Usually, a
volume of 5–10 times the
column volume will be
sufficient.

High baseline drift The solvent is degraded or Before you start an analysis,
(Cont'd) inhomogeneous. be sure that the eluents are
already homogenized in the
reservoirs. Use fresh solvent
and check the eluent filter
frits. In aqueous solvents,
growth of microorganisms is
possible.
The environmental conditions are Make sure that the
unstable. temperature and the humidity
are constant. Avoid draft.
Verify on the detector that the
lamp and flow cell covers are
properly installed and that the
front panel door is closed.
The mobile phase is delivered in Direct the mobile phase to
circles. waste.
For additional causes, refer to the → Detector manual
detector.
High noise level, non-periodic The solvent is degraded or of Use fresh and appropriate
baseline fluctuation poor quality/purity. solvents (HPLC-quality).
detector.
Periodic baseline fluctuation, There are pressure fluctuations Purge the pump
pulsation from the pump. (→ Operating Instructions).
There are air bubbles in the Purge the pump
system. (→ Operating Instructions).
When acetonitrile is used with Consider replacing the
pumps that have sapphire check sapphire cartridges with the
valve cartridges installed, valve ceramics cartridges
problems may occur; for (→ page 49).
example, higher pulsation is
observed.
ISO-3100BM Replace the pulse damper
The membrane in the pulse (→ page 121) or pulse
damper is defective. damper membrane
(→ page 122).
Pressure pulsation or The solvent is not degassed Degas the eluent.
inconstant pressure sufficiently.
The solvent is degraded. Use fresh solvent.

Pressure pulsation or There are pressure fluctuations Purge the pump

inconstant pressure (Cont'd) from the pump. (→ Operating Instructions).
Check the compression
values and take remedial
action (→ page 148).
system. (→ Operating Instructions).
The inlet valve or outlet valve on Clean or replace the check
the working cylinder is dirty. valve cartridges (→ page 49).
After a maintenance procedure Remove and reinstall the
(replacement of pump head, pistons (→ pages 60 and 62).
piston, or piston seal) the pistons
are not in the appropriate position
for normal operation.
blocked. (→ page 92or 94).
DGP-3600BM, you can
ISO-3100BM Replace the pulse damper
The membrane in the pulse (→ page 121) or pulse
damper is defective. damper membrane
(→ page 122).
Peak tailing The extra column volume is too Use short capillaries. Use
large. capillaries with an appropriate
inner diameter (0,13 mm with
UHPLC columns; 0,18 mm
with conventional HPLC
columns)
There are bad capillary Replace the capillaries.
connections. Consider installing Viper
capillaries.
Peak Broadening, The inner diameter of the Change the capillary. Use a
increased dead time capillary to the detector is too capillary with an appropriate
large. inner diameter.
The filter frits on the solvent line Check the solvent line filters
filters are clogged. for permeability. Replace the
filter frit if necessary
(→ Operating Instructions).
The capillaries are clogged or Replace the capillaries.
there are bad capillary Consider installing Viper
connections. capillaries.
The sample loop is clogged. Replace the sample loop
(→ Autosampler manual).

Peak Broadening, The proportioning valve is Replace the proportioning

increased dead time (Cont'd) defective. valve (→ page 97).
The column is overloaded or Clean or replace the column.
contaminated.
The solvent is degraded. Use fresh solvent.
Reproducible ghost peaks The degassing channels are Rinse the degassing channels.
appear in the chromatogram. contaminated.
The solvents are old, degraded or Use fresh and appropriate
of poor quality/purity. solvents (HPLC-quality).
Contamination occurs Flush the system using an
somewhere in the system. appropriate solvent.
Additional peaks appear in With gradients, the equilibration Increase the equilibration
the injection peak. time after the flush cycle is too time.
short.
The dead volume is too high. Eliminate the dead volume.
Spikes There is electrical interference Separate the electrical circuit
from other modules. from strong current
consumers. Consider
installing an uninterruptible
power supply (UPS).
The column temperature is Install a restrictor at the flow
significantly above the boiling cell outlet. If possible, use a
point of the mobile phase. post-column cooler (→ TCC
manual).
detector.
Negative peaks The sample solvent and mobile Dissolve the sample in the
phase differ in composition. mobile phase.
Absorption of the solute is lower Select a different wavelength.
than the absorption of mobile Use a mobile phase with less
phase. UV background absorption.
Poor peak area precision The capillary connections are not Check and tighten the
installed properly or they are not capillary connections.
tight. Consider installing Viper
capillaries.
Exchange the needle port if
necessary (→ Autosampler
manual).
Exchange the needle if
necessary (→ Autosampler
manual).

Poor peak area precision There are dead volumes in the Replace the fittings. Make
(Cont'd) capillary connections. sure that the capillaries are
connected correctly. Consider
installing Viper capillaries.
The piston seals are not sealing Replace the piston seals
tight. (→ page 65).
working cylinder. (→ Operating Instructions).
There is pump pulsation. Use degassed solvents.
Check the Compression
value (→ page 148).
The gradient is irreproducible. Change the gradient.
Check the pump function and
degassing.
Check the filter frits in the
solvent line filters for
contamination and exchange
as necessary.
There are shifts in retention Check the Compression
times. value (→ page 148).
The sample is unstable and Use new sample or change
decomposes. the conditions. Thermostate
the sample in the autosampler
if possible.
Baseline fluctuations See the remedial actions
provided in the related
baseline sections further up in
this table.
The environmental conditions are Make sure that the
unstable. temperature and the humidity
are constant. Avoid draft.
Consider using a column
compartment.
Contamination occurs Flush the system with an
For additional causes, refer to the → Autosampler manual
autosampler.
No flow The system is leaking. Find and eliminate the leak.
The solvent is old, degraded, or Use fresh and appropriate
of poor quality. solvents (HPLC-quality).
Contamination occurs Flush the system with an

No flow One or both check valve Verify that the cartridges are
(Cont'd) cartridges are not installed installed in the direction of
correctly or are defective. flow. Replace the cartridges
(→ page 49).
solvent or in the pump heads. (→ Operating Instructions)
and check the degasser
blocked. (→ page 92or 94).
DGP-3600BM, you can
There are air bubbles in the flow Perform a wash cycle
path. (→ Autosampler manual).
Non-degassed wash solution
is used. Degas the wash
solution (→ Autosampler
manual).
After a maintenance procedure Remove and reinstall the
(replacement of pump head, pistons (→ pages 60 and 62).
piston, or piston seal) the pistons
are not in the appropriate position
for normal operation.
Flow fluctuation The inlet path is blocked. Check the inlet lines, filter of
the pump, proportioning valve
etc. for signs of blockage.
There are air bubbles in the inlet Purge the pump
path. (→ Operating Instructions for
the pump) and check the
degasser
The check valves are dirty or Replace the check valve
defective. cartridges (→ page 49).
The piston does not contact the Check whether the piston is
magnet holder. installed correctly. Reinstall
the piston (→ page 69).
The piston seals are not sealing Replace the piston seals
tight. (→ page 65).

Poor degassing There is a leak in the capillaries Inspect the capillary and
or solvent supply lines or there solvent tube connections for
are loose connections. leakage; tighten loose fitting
connections.
The degasser is not working Inspect the degassing
properly. chamber for indications for
leakage. Check the degasser
vacuum.
The flow rate is too high. Reduce the flow rate.
Degasser noise The vacuum pump of the Fill and degas all channels
degasser is running at high speed. (even if they are not used for
the application).

7.6 Checking the Compression Values

The compression value of the pump head can provide valuable information for
troubleshooting. If you observe pump pulsation and/or shifts in retention times, check the
compression values:
• On the pump display, select the Diagnostics menu and Compression right or
Compression left.
• In Chromeleon, open the Commands dialog box for the pump and select [Pump Device
Name]_Wellness_LeftBlock and Compression or [Pump Device
Name]_Wellness_RightBlock and Compression. (For information about the [Pump
Device Name], see page 24.
The values are indicated in percent; they show the compression of the last stroke. The
compression value should be lower than 100% and stable. With isocratic conditions, the
compression value should remain stable. When running a gradient, the compression value
changes proportionally to the pressure curve. If the value is close to 100%, the required
precompression might not be reached and pulsation may occur.
The table shows guiding values for the some common solvents:
Solvent Compression*
RS Pumps SD Pumps - Working Pressure
Max. 62 MPa Max. 50 MPa
Acetonitrile 45 % ± 10 % 70 % ± 10 % 80 % ± 10 %
Methanol 50 % ± 10 % 70 % ± 10 % 85 % ± 10 %
Water 25 % ± 10 % 50 % ± 10 % 50 % ± 10 %
Solvent Compression*
BX Pump BM Pumps
Acetonitrile 23 % ± 5 % 75 % ± 10 %
Methanol 23 % ± 5 % 80 % ± 10 %
Water 18 % ± 5 % 55 % ± 10 %
* with 100 % of degassed solvent delivered at 40 MPa (BX pump: 10 MPa), pump purged and stable for some time
The values are linear to the pressure.
• If the compression value is lower than indicated in the table, the valve cartridge (outlet
check valve) may be defective. Replace the cartridge (→ page 49).
• If the compression is higher than indicated in the table:
♦ The piston seals may be defective. Replace the piston seals (→ page 65).
♦ The valve cartridge (inlet check valve) may be defective. Replace the cartridge
(→ page 49).
• If the compression is very high and the pressure is low, there may be air bubbles in the
system. Purge the pump (→ Operating Instructions for the pump).

7.7 Checking the Pressure Transducers

This section is intended to help you identify and eliminate the source for problems related
to pressure transducer malfunctions, such as
• The pressure is not down to zero when the purge valve is open.
• Pulsation is observed for a HPG-3x00 pump.
• Messages related to the pump pressure or to a pressure sensor appear on the pump
display or in the Chromeleon Audit Trail, for example
♦ Pressure sensor malfunction
♦ Can't reset pressure sensor. Pressure is out of range.
—or—
Can't reset pressure sensor. Pressure is not constant.
♦ Working piston zero pressure out of range
First, identify the pressure transducer for which the problem occurs. Note the following:
• If the purge valve is open, but the pressure is not down to zero, the message refers to the
pressure transducer for the system pressure. The pressure transducer for the system
pressure is integrated in the purge unit.
• Pulsation of a HPG-3x00 pump may be observed also if the pressure transducer in one of
the pump heads is not working properly.
If a message appears, it depends on the firmware version of the pump whether the
message text on the pump display indicates also the pump block for which the message
appears (firmware version ≤ 2.40). If it does not, check the Audit Trail message for the
block information.
If the block information is not available, identify the pump block as follows: Have one
block deliver 100%. If the message does not appear for this block, have the second block
deliver 100% to verify that the message appears for the second block.
After you have identified the pressure transducer, verify that the problem does not occur
due to a mechanical fault. Continue with the following steps:
1. Check the pressure transducer cable and connector for proper contact.
Unplug the pressure transducer cable from the connector on the interior front panel.
Reconnect by pushing the cable firmly into the connector to make sure that it is properly
seated.
2. Check the pressure transducer offset.
If you are sure that the cable is properly seated in the connector, perform the
SetPressureZero command from the Commands dialog box in Chromeleon
(→ page 24). If a 'Can't reset pressure sensor message' appears, the pressure transducer
may be leaking or the cable may be defective.

3. Verify that the pressure transducer is tight and that the cable is not defective.
a) Set a multimeter to DC and to the highest sensitivity.
b) Attach one multimeter probe to the purge unit or pump head, depending on the
pressure transducer for which the problem occurs.
c) Attach the other probe to a pin of the cable plug.
Fig. 97: Attaching the multimeter probes to the pressure transducer and cable plug
d) Check all pins one after the other. The voltage must remain below 10 mV.
♦ If it does not, the sensor is leaking.
Replace the purge unit (→ page 45) or pump head (→ page 60), depending
on the component that accommodates the pressure transducer.
♦ If it does, check the pressure transducer cable (see next step).
e) With the multimeter, verify that pins 1 through 4 of the cable are connected to each
other. (Pins 5 and 6 are not used.) The typical resistance is about 10 kΩ.
Fig. 98: View into the pressure transducer cable plug
If the contact between one of the pins and the other pins is broken, the cable is
defective. Replace the purge unit (→ page 45) or pump head (→ page 60), depending
on the component that houses the pressure transducer.
4. If no mechanical fault is detected, the electronics may be defective. Replace the purge
unit (→ page 45) or pump block (→ page 103) as appropriate.

7.8 Recording the Service Channels/Contacting Support

If you are unable to eliminate a problem, record the service channels for the pump. They
can provide helpful information to identify and eliminate the source for the problem.
Before you begin, verify that

• Chromeleon 6.80 Service Release 9 or later is installed.
If you have to record the service channels with a Chromeleon version earlier than Service
Release 9, contact your local Thermo Fisher Scientific support center or the support
center authorized by Thermo Fisher Scientific for your region.
• An appropriate version of service panel for the pump is available. The button for
recording the service channels is available only on the service panels for the pump
(= UltiMate_3000_RS_SD_BM_BX_Pumps_Service.pan) with a file date later than
November 2009.
For information about how to restore and access a service panel, see section 5.4
(→ page 26).
1. Start the Chromeleon Server Monitor.

2. Start the Chromeleon Server Configuration program in the debug mode:
a) Open a Command Prompt input box. (Under Windows™ XP, the path is Start >
Programs > Accessories > Command Prompt. Under Windows™ 7, the path is the
same; however, right-click Command Prompt and then click Run as
administrator.)
b) Navigate to the Bin directory of the Chromeleon installation (→ Fig. 99).
c) Enter cminst /debug (→ Fig. 99) and press Return to start the Server Configuration
program.
Syntax Action
(drive letter): Changes the drive (here to drive D:)

Cd [folder] Opens the specified folder
(here: Chromel\Bin)
Tip: To see the content of the current directory, type Dir/w.
Fig. 99: Command prompt and syntax
3. Right-click the pump in the timebase and click Properties on the menu.

4. On the Debug page, select the Activate Default Service Channels check box. (The
Debug page is available only if you start the Server Configuration program in the
debug mode.)
Fig. 100: Debug page
If the Activate Default Service Channels check box is not visible, the pump has been
installed and configured with a Chromeleon version earlier than Chromeleon 6.80
Service Release 9. In this case, delete and reinstall the pump in the Server Configuration
program.
5. In the Chromeleon client program, open the service panel for the pump.
6. Start the batch for which the problem was observed.
7. On the service panel, in the Sub Panels section, click Record Debug Channels.
8. Important
Before proceeding with the next step, verify that the retention time is positive. Only then,
the service channels will be recorded properly.
9. On the DebugChannels panel, click Record ON to start recording the service
channels. (A message appears stating that you are intercepting a running batch.
Confirm the message by clicking OK; the message does not affect channel recording.)
Tip: If you started recording by mistake at a negative retention time, stop

recording by clicking Record OFF. When the retention time is positive,
click Record ON again to restart recording.

10. Before the sample is finished, click Record OFF. (A message appears stating that you
are intercepting a running batch. Confirm the message by clicking OK; the message
does not affect channel recording.)
This is important as otherwise
♦ The sample may remain 'running'.
♦ The channels will not be recorded for the next sample.
11. Record the channels until the problem is observed.
If it is not observed with the first sample, continue with the next samples. You have to
start and stop recording for each sample as described above.
12. Create a Chromeleon backup file (*.cmb) that includes the recorded information and
any other information that may be helpful, for example, the Audit Trails.
For details about how to create a Chromeleon backup file, refer to the Chromeleon Help.
13. Important
a) In the Server Configuration program, clear the Activate Default Service Channels
check box in the Server Configuration program. This is possible only while you are in
the debug mode. The debug mode is disabled when you exit the Chromeleon Server
b) Exit and close the Command Prompt input box if it is still open.
14. Contact your local Thermo Fisher Scientific support center or the support center
authorized by Thermo Fisher Scientific for your region.
Enter a case as requested by the escalation process.

8 Updating the Pump Firmware

The pump is shipped with the most recent firmware version. The pump firmware is also
included in Chromeleon.
To check which firmware version is installed in the pump and which version is included in
Chromeleon:
• Firmware version installed in the pump

- Turn on the pump via the main power switch on the rear panel to see general
information, about the pump, including the firmware version, on the front panel display.
- On the pump display, select the Diagnostics menu and select Firmware version.
• Firmware version in Chromeleon
- In the Server Configuration program, open the configuration pages for the pump. On
the General page, the firmware version is displayed.
- In the Windows Explorer, locate the IQReport.log file in the IQ folder of your
Chromeleon installation. In the file, search for Pump3x00RS.hex (also for SD, BM, and
BX pumps).
Tip: When updating the pump firmware from Chromeleon, this information will
also be provided during the download (see later in this section).
Whenever a new firmware version is released for the pump, the new version is provided
with the next Chromeleon Service Pack release and described in the release notes.
The new firmware will not be downloaded automatically to the pump when you install a
Chromeleon Service Release. To update the firmware in the pump, follow these steps:
Important: For the download to be successful, the communication between the

pump and Chromeleon must not be interrupted during the download and
the pump must not be turned off.
1. Before you begin, verify that

♦ The pump is connected in Chromeleon.
♦ The Chromeleon server is in running idle mode. All processes on the Chromeleon
server PC and in Chromeleon have been stopped.
♦ The pump is unpressurized (for example, the purge valve is open) and the flow is
turned off.
2. Start the Server Configuration program.
3. Right-click the pump in the timebase and click Properties on the menu.

4. On the General page, the firmware version provided in Chromeleon for the pump is
displayed in the Firmware field. If more than one firmware version is available for the
pump in Chromeleon, select the version you wish to transfer from the Firmware list.
5. Click Download. A message displays the firmware version that is currently installed
in the pump and the version that will be downloaded from Chromeleon
Tip: If the pump comes with a newer firmware than the version included in
Chromeleon, do not downgrade the firmware. Older firmware may be
incompatible with new hardware revisions.
6. Click Yes to start the download. (Click No to cancel the action.)
The download may take several minutes. The download is complete when Firmware
download completed successfully appears in the Messages Server window in the
Chromeleon Server Configuration program. The message appears also in the Chromeleon
Audit Trail.
Immediately after the new firmware has been downloaded from Chromeleon to the pump, the
pump performs a reset. For about 15 seconds, the internal boot loader is updated. Do not turn
off the pump while the boot loader is updating.
If the download is not successful, one or more messages appear in the Audit Trail. In this
case, turn off the pump. Turn on the pump again and repeat the steps above. If the download
fails again, consider replacing the main board (→ page 100).

9 Interior Components and Flow Path

The sections listed in the table provide a short overview of the interior components and
liquid flow path through the pump.
For the … Find information about the ... On page …
ISO-3100SD Interior components 157

ISO-3100BM Interior components 159
LPG-3400 Interior components 161
DGP-3600 Interior components 164

9.1 ISO-3100
9.1.1 ISO-3100SD
9.1.1.1 Interior Components

9 8 7 6
1
2
3 4 5 3
Fig. 101: Interior components (ISO-3100SD)
No. Description
1 Peristaltic pump
2 Detector of the rear seal wash system
3 Capillary guides
4 Pump head with working cylinder and equilibration cylinder
5 Leak sensor
6 Inline filter
7 Purge unit with purge valve and pressure transducer for the system pressure
8 Pump lights (here hidden by the front panel door)
9 Pump block status LED (→ page 125)

9.1.1.2 Flow Path
The picture illustrates the liquid flow path through the pump.
Fig. 102: Flow path through the ISO-3100SD
SRD-3x00 Solvent Rack with analytical vacuum degasser → Operating

Instructions
1 Solvent supply line from analytical degasser to pump head 6030.2546
2 Pump head with working cylinder and equilibration cylinder → section 6.10,
(entire assembly) page 51
3 Capillary from working cylinder to equilibration cylinder (U-tube) Included in
6040.3001
4 Capillary from pump head to purge unit Included in
6040.3001
5 Purge unit with purge valve and system pressure transducer 6040.7165A
6 Capillary from purge unit to inline filter 6040.3024
7 Inline filter 6040.5110
Note: Tubing and capillaries are always shipped with the appropriate fitting connections.

9.1.2 ISO-3100BM
9.1.2.1 Interior Components

10 9 8
1 7
2
3 4 5 3
6
Fig. 103: Interior components (ISO-3100BM)
No. Description
1 Peristaltic pump
3 Capillary guides
5 Leak sensor
6 Pulse damper
7 Inline filter (→ page 92)

9.1.2.2 Flow Path

Fig. 104: Flow path through the ISO-3100BM
SRD-3x00 Solvent Rack with analytical vacuum degasser (optional) → Operating Instructions
1 Solvent supply line from analytical degasser to pump head 6030.2546
—or—
Solvent supply line from solvent reservoir to pump head 6030.2548
2 Pump head with working cylinder and equilibration cylinder → section 6.10, page 51
(entire assembly)
3 Capillary from working cylinder to equilibration cylinder (U-tube) Included in 6042.3002
4 Capillary from pump head to purge unit Included in 6042.3002
5 Purge unit with purge valve and system pressure transducer 6042.7155A
6 Capillary from purge unit to pulse damper Included in 6042.3002
7 Pulse damper for pumps with serial numbers:
Up to 8086057 6042.6000
8086058 and higher 6042.6010
8 Capillary from pulse damper to inline filter Included in 6042.3002
9 Inline filter 6040.5110

9.2 LPG-3400
9.2.1 Interior Components
12 11 8 10 9 8
1 7
2
6
3 4 5 3
Fig. 105: Interior components (here LPG-3400SD)
Instead of the two-step mixing system, LPG-3400BM

pumps have an inline filter, which is located below
the pump head. 9
6
Fig. 106: Inline filter (LPG-3400BM)
No. Description
1 Peristaltic pump
3 Capillary guides
5 Leak sensor
6 LPG-3400SD and RS: Capillary mixer (→ page 85)
LPG-3400BM: Capillary from purge unit to inline filter
7 4-channel vacuum degasser
9 LPG-3400SD and RS: Static mixer (→ page 85)
LPG-3400BM: Inline filter
12 4-channel proportioning valve

9.2.2 Flow Path
Fig. 107: Flow path through the LPG-3400 (top: LPG-3400SD/RS, bottom: LPG-3400BM)

SR-3000 Solvent Rack → Operating Instructions

1 Solvent supply lines to degasser (4 lines) 6040.2049
2 Analytical 4-channel vacuum degasser 6030.9289
Note: You are free to choose the direction of flow through the (degassing chamber)
degasser.
3 Tubing from degasser to proportioning valve (4 lines) 6040.2540
4 4-channel proportioning valve 6040.4025
5 Tubing from proportioning valve to pump head (pack of 2 tubes)
LPG-3400RS and LPG-3400SD 6040.3023
LPG-3400BM 6042.3023
(entire assembly)
7 Capillary from working cylinder to equilibration cylinder (U-tube)
LPG-3400RS (S/N ≥ 8030113) Included in 6040.3003
LPG-3400RS (S/N < 8030113) and LPG-3400SD Included in 6040.3001
LPG-3400BM Included in 6042.3001
8 Capillary from pump head to purge unit
LPG-3400RS (S/N ≥ 8030113) Included in 6040.3003
LPG-3400RS (S/N < 8030113) and LPG-3400SD Included in 6040.3001
LPG-3400BM Included in 6042.3001
9 Purge unit with purge valve and system pressure transducer
LPG-3400SD 6040.7165A
LPG-3400RS and LPG-3400BM 6042.7155A
10 LPG-3400RS and LPG-3400SD: Capillary mixer → page 85
LPG-3400BM: Capillary from purge unit to inline filter 6042.3024 and also
Included in 6042.3001
11 LPG-3400RS and LPG-3400SD: Static mixer → page 85
LPG-3400BM: Inline filter 6042.5014

9.3 DGP-3600

11 10 9 8 11 10 9 8
1
2
3 4 5 6 7 3 4 5 6
Fig. 108: Interior components (here DGP-3600 SD/RS)
Instead of the two-step mixing system, DGP-3600BM

pumps have an inline filter, which is located under the
pump head. 10
5
Fig. 109: Inline filter (DGP-3600BM)
No. Description
1 Peristaltic pump
3 Capillary guides
5 DGP-3600SD and RS: Capillary mixer (→ page 85)
DGP-3600BM: Capillary from purge unit to inline filter
7 Leak sensor
10 DGP-3600SD and RS: Static mixer (→ page 85)
DGP-3600BM: Inline filter
11 3-channel proportioning valve

9.3.2 Flow Path
Fig. 110: Flow path through the DGP-3600 (top: DGP-3600SD/RS; bottom: DGP-3600BM)

SRD-3x00 Solvent Rack with analytical vacuum degasser → Operating Instructions

1 Solvent supply lines from degasser to proportioning valve (3 lines) 6030.2547
2 3-channel proportioning valve 6040.4026
3 Tubing from proportioning valve to pump head (pack of 2 tubes)
DGP-3600RS and DGP-3600SD 6040.3023
DGP-3600BM 6042.3023
(entire assembly)
DGP-3600RS (S/N ≥ 8030113) Included in 6040.3003
DGP-3600RS (S/N < 8030113) and DGP-3600SD Included in 6040.3001
DGP-3600BM Included in 6042.3001
6 Capillary from pump head to purge unit
DGP-3600RS (S/N ≥ 8030113) Included in 6040.3003
DGP-3600RS (S/N < 8030113) and DGP-3600SD Included in 6040.3001
DGP-3600BM Included in 6042.3001
DGP-3600SD 6040.7165A
DGP-3600RS and DGP-3600BM 6042.7155A
8 DGP-3600RS and SD: Capillary mixer → page 85
DGP-3600BM: Capillary from purge unit to inline filter 6042.3024 and also
Included in 6042.3001
9 DGP-3600RS and SD: Static mixer → page 85
DGP-3600BM: Inline-Filter 6042.5014

9.4 HPG-3200

8 7 10 9 8 7
1
2
3 4 5 3 4
Fig. 111: Interior components (here HPG-3200SD)
No. Description
1 Peristaltic pump
3 Capillary guides
5 Leak sensor
7 Pump lights (here hidden by the open front panel door)
9 Capillary mixer (HPG-3200SD and HPG-3200RS)
Capillary from purge unit to static mixer (HPG-3200BX)
10 Static mixer

9.4.2 Flow Path
Fig. 112: Flow path through an HPG-3200

With an HPG-3200BX, the solvent supply lines are connected directly to the pump inlet.
No. Description Part No.*
Only HPG-3200SD and HPG-3200RS

Only HPG-3200BX
Solvent supply lines to pump inlet 6042.2530
1 Only HPG-3200SD and HPG-3200RS
Solvent supply lines from analytical degasser to pump head (pack of 2 6035.2530
lines)
(entire assembly)
HPG-3200RS (S/N ≥ 8030113) Included in 6040.3002
HPG-3200RS (S/N < 8030113) and HPG-3200SD Included in 6040.3000
HPG-3200BX Included in 6042.3005
4 Capillary from left pump head to purge unit


HPG-3200SD 6040.7155A
HPG-3200RS and HPG-3200BX 6042.7157A
6 Capillary mixer (HPG-3200SD and HPG-3200RS) → page 85
Capillary from purge unit to static mixer (HPG-3200BX) Included in 6042.3005
7 Static mixer → page 85
8 Capillary from right pump head to purge unit
* These part numbers also include the appropriate fittings and ferrules.

9.5 HPG-3400
9 8 7 11 10 9 8 7
1
2
3 4 5 3 4
Fig. 113: Interior components (here HPG-3400SD)
No. Description
1 Peristaltic pump
3 Capillary guides
5 Leak sensor
7 Pump lights (here hidden by the open front panel door)
9 Solvent Selector "2 from 4"
10 Capillary mixer
11 Static mixer

9.5.2 Flow Path
Fig. 114: Flow path through an HPG-3400

1 Solvent supply lines from analytical degasser to solvent selectors 6035.2532
(pack of 4 lines)
2 Solvent Selector "2 from 4" 6040.2611
3 Tubing from solvent selector to pump head (pack of 2) 6040.3017
(entire assembly)
5 Capillary from working cylinder to equilibration cylinder (U-tube) Included in
HPG-3400RS (S/N ≥ 8030113) 6040.3002
HPG-3400RS (S/N < 8030113) and HPG-3400SD 6040.3000
6 Capillary from left pump head to purge unit Included in
HPG-3400RS (S/N ≥ 8030113) 6040.3002
7 Purge unit with purge valve and pressure transducer'
HPG-3400SD 6040.7155A
HPG-3400RS 6042.7157A

8 Capillary mixer → page 85

9 Static mixer → page 85
10 Capillary from right pump head to purge unit Included in
HPG-3400RS (S/N ≥ 8030113) 6040.3002
* These part numbers also include the appropriate fittings and ferrules.

10 Optimizing the Pump for Specific Applications

Only RS and SD pumps (except ISO-3100SD)
In the standard configuration, the pump has been optimized for the gradient delay volume and
ripple.
The gradient delay volume of an HPLC system is defined as the volume that the pump must
deliver until a change in solvent composition reaches the head of the column. Reducing the
gradient delay volume allows changes in the solvent composition to become effective on the
column much earlier, thus having a faster effect on the elution of analytes. For information
about the gradient delay volume of the pumps, see the Technical Information section in the
Operating Instructions for the pump.
For gradient separations at low flow rates (for example, less than 100 µL/min) or very steep
gradients, you can adapt the gradient delay volume to your requirements, by replacing the
two-step mixing system with which the pump is shipped with either a two-step mixing system
with lower volume or the single-step mixing system. However, in both cases, the ripple will
increase.
In addition, two-step mixing systems with a higher volume are available for the pump.
Operating the pump with these mixing systems will reduce the ripple but increase the gradient
delay volume.
For more information about the two-step mixing systems, see the next page. For information
about the single-step mixing system, see page 175.

Two-step mixing systems (SpinFlow)
Two-step mixing system
Mixing volume including: Part No.

100 µL Static mixer (volume: 75 µL)
Capillary mixer (volume: 25 µL)
RS pumps (S/N < 8030113), SD pumps 6040.5100
RS pumps (S/N ≥ 8030113) 6042.51001
200 µL Static mixer (volume: 150 µL) 6040.51102
1
The capillary mixer in this system is a Viper, MP35N mixer. This mixer cannot be used with RS pumps
with serial numbers < 8030113. With these pumps, the mixing system with stainless steel capillary mixer
(part no. 6040.5100) must be used.
² The part number is for all pump types.
The capillary mixer is not included in the shipment—For details, see Mixing systems with a mixing
volume > 100 µL under this table.
If you want to replace the mixing system with which the pump is shipped (→ page 85)
with a system mentioned in the table, observe the following:
• Mixing systems with a mixing volume > 100 µL

For these mixing systems, you need not replace the capillary mixer. The capillary mixer
is the same in both mixing systems, and thus the mixer is not included in the shipment. If
you want to replace the capillary mixer nevertheless, see page 85 for the correct part
numbers.
• Mixing system with a mixing volume of 100 µL
If you want to use this mixing system, you have to replace both mixers. Therefore, both
mixers are included in the shipment.
The mixer shipment includes all components required for the installation and detailed
instructions.

Single-step mixing system
Single-step mixing system Part No.

SD pumps (except ISO-3100SD)and RS pumps (S/N < 8030113)
Single-step mixing system, stainless steel, mixing volume: 35 µL, 6040.5000
including:
Capillary mixer (25 µL) and
Inline filter (10 µL, porosity of the filter frit: 10 µm)
The components of the kit are available also as separate parts:
Capillary mixer, stainless steel (volume: 25 µL) 6040.3020
Inline filter, stainless steel (volume: 10 µL, porosity of the frit: 10 µm) 6040.5010
Replacement frits for inline filter (2 frits, porosity: 10 µm) 6268.0034
RS pumps( S/N ≥ 8030113)
Single-step mixing system, titanium/MP35N, mixing volume: 35 µL, 6042.5000
including:
Capillary mixer, Viper, MP35N (volume: 25 µL) and
Inline filter, titanium (volume: 10 µL, porosity of the filter frit: 2 µm)
The components of the kit are available also as separate parts:
Capillary mixer, Viper, MP35N (volume: 25 µL) for 6042.3020
Inline filter, titanium (volume: 10 µL, porosity of the frit: 2 µm) 6042.5014
Replacement frits for inline filter (2 frits, porosity: 2 µm) 6268.0036
1. Remove the static mixer as described in section 6.13.1 (→ page 85).

2. Disconnect the capillary mixer on the purge unit.
3. Connect the replacement capillary mixer (25 µL) to the purge unit.
4. Connect the free end of the capillary mixer with the inline filter and place the inline
filter below the right pump head in the pump enclosure.
Fig. 115: Single-step mixing system with inline filter (here in an HPG-3x00)
5. Connect an appropriate capillary for the connection to the autosampler on the mixer
outlet.

6. In Chromeleon, set the StaticMixer property to InlineFilter_10 µL. If the property is

not set to the correct value, the leak tests (→ page 18) may not provide reliable results.
Tip: Check the permeability of the frit in the inline filter at regular intervals (the
procedure is similar to the steps in section 6.14.3, page 96).
To replace the filter frit, follow the steps in section 6.14.2.2 (→ page 95).

11 Connecting a Corona or Colouchem III Detector

Note the following if you want to connect a Corona or Coulochem detector to the pump:
• To connect the detector to a digital I/O port on the rear panel of the pump, use the 6-pin
mini-DIN signal cable (part no. 6000.1004).
• Verify that firmware version 2.20 or later is installed in the pump. Update the firmware if
necessary (→ page 153).
• For information about how to connect the detector to the pump, follow the instructions in
the Chromeleon Help. The Help provides separate sections for connecting these detectors
to an UltiMate 3000 system pump, including details about the appropriate cabling and the
settings required in the Server Configuration program.
• Only Corona
If an error occurs in the detector, pumping is stopped and a message appears on the pump
display and in the Chromeleon Audit Trail ('An emergency stop was requested over the
digital input line') only if the pump and detector are connected and configured as
described in the Chromeleon Help. Check the detector and take appropriate remedial
action (→ Detector manual).
• Only Coulochem III
When the pump and detector are connected and configured as described in the
Chromeleon Help, the detector cell is automatically turned off when the pump flow is
zero.
For more information, see the Chromeleon Help.

12 Consumables and Spare Parts

Spare parts and accessories are always maintained at the latest technical standard. Therefore,
part numbers are subject to alteration. However, updated parts are always compatible with
the parts they replace.
The spare parts listed below are mentioned in this manual. The maintenance instructions for
UltiMate 3000 HPLC Systems ('UltiMate 3000 and OEM Maintenance Instructions') may
contain more numbers, especially for parts used in special instrument configurations.
The part number always refers to the packing unit.

Unless stated otherwise, the packing unit is 1.
Adapter board for SRD-3x00 Solvent Racks (serial numbers ≤ 8003272) 6030.0004
It may be necessary to update first generation SRD-3x00 Solvent Racks for operation
with second-generation UltiMate 3000 pumps.
Cap seal assembly tool (to be used for cap seal 6005.1301) 6300.0201
Cap seal for purge valve knob (to be used only with purge valve knobs 6040.2030, that 6005.1301
is, for knobs without integrated cap seal, see 48.)
Capillary, nanoViper (length: 750 mm, inner diameter: 75 µm) 6041.5780
To be used, for example, when running in new piston seals
Capillary (PEEK, 1/16", I.D. 0.75 mm I.D.) for HPG-3200BX 2251.6003
(without fitting connections)
Use the following fittings with this capillary: Rheflex fittings (PEEK, 1/16", finger-
tight), part no. 6000.0012 (10 fittings).
Capillary (PEEK, 1/16", 0.25 mm I.D.), BM pumps 6251.6001
Capillary from purge unit to inline filter (including fitting connections) for
ISO-3100SD 6040.3024
LPG-3400BM and DGP-3600BM 6042.3024
Capillary from working cylinder to equilibration cylinder (U-tube), Viper 6040.3008
Available as an option for SD and RS pumps (S/N < 8030113)
If, with flow rates of < 2 mL/min, leakage is observed around the connection ports of
the U-tube with which the pump is shipped, consider installing this U-tube with Viper
connections. (Note that the capillary is not intended for use with flow rates higher than
2 mL/min.)
Capillary mixer → Mixer, capillary mixer
Capillaries, kit for ISO-3100BM, including: 6042.3002
1 capillary from working cylinder to equilibration cylinder (U-tube)
1 capillary from pump head to purge unit
1 capillary from purge unit to pulse damper
1 capillary from pulse damper to inline filter

Capillaries, kit for LPG-3400BM and DGP-3600BM, including: 6042.3001

2 capillaries from working cylinder to equilibration cylinder (U-tubes)
2 capillaries from pump head to purge unit
2 capillaries from purge unit to inline filter
Note: The capillary from purge unit to inline filter can be ordered also separately
(part no. 6042.3024).
Capillaries, SST, kit for HPG-3x00RS (< S/N 8030113) and HPG-3x00SD, 6040.3000
including:
1 capillary from left pump head to purge unit
1 capillary from right pump head to purge unit
Capillaries, SST, kit for ISO-3100SD, LPG-3400SD, LPG-3400RS (< S/N 8030113), 6040.3001
DGP-3600SD, DGP-3600RS (< S/N 8030113), including:
2 capillaries from head to purge unit
Capillaries, Viper, MP35N, kit for HPG-3x00RS (≥ S/N 8030113), including: 6040.3002
Capillaries, Viper, MP35N, kit LPG-3400RS and DGP-3600RS (≥ S/N 8030113), 6040.3003
including:
2 capillaries from head to purge unit
Capillaries, set for HPG-3200BX, including: 6042.3005
1 capillary from purge unit to static mixer

Capillaries, Viper, for UltiMate 3000 RSLC systems (single stack setup)
A—Viper capillaries
Capillary (0.13 x 550 mm (I.D. x L), SST, Viper) 6040.2305
e.g. for the connection from the RS pump to the WS-3000RS
e.g., for the connection from the HPG-3x00RS pump to the WPS-3000RS
e.g., for the connection from the WPS-3000RS to the TCC-3000RS (column inlet)
e.g., for the connection from the TCC-3000RS (column outlet) to the DAD-3000RS,
MWD-3000RS, or VWD-3400RS
B—Viper capillary kits
Viper capillary kit for RSLC systems with a LPG-3400RS or DGP-3600RS pump 6040.2301
The kit includes the capillaries with part numbers 6040.2325, 6040.2335, and
6040.2365 (see 'A—Viper capillaries' for details).
Viper capillary kit for RSLC systems with an HPG-3200RS or HPG-3400RS pump 6040.2308
Capillaries, Viper, for UltiMate 3000 standard systems (single stack setup)
e.g., for the connection from the ACC-3000 to the column inlet
e.g., for the connection from the SD pump to the WPS-3000SL
e.g., for the connection from the WPS-3000SL to the TCC-3000SD (column inlet)
e.g., for the connection from the TCC-3000SD (column outlet) to the DAD-3000,
MWD-3000, or VWD-3100
Viper capillary kit for standard systems with an ISO-31000SD, LPG-3400SD, or 6040.2302
DGP-3600SD
6040.2385 (see 'A—Viper capillaries' for details). 6040.2309
Viper capillary kit for standard systems with an HPG-3200SD or HPG-3400SD

Capillaries, Viper, for UltiMate 3000 Bio-RS systems (Single Stack setup)
Capillary (0.18 x 550 mm (I.D. x L), MP35N,Viper 6042.2355
e.g. for the connection from the RS pump to the WPS-3000TBRS
Capillary (0.10 x 350 mm (I.D. x L), MP35N, Viper 6042.2340
e.g. for the connection from the WPS-3000TBRS to the TCC-3000RS (column inlet)
Capillary (0.10 x 250 mm (I.D. x L), MP35N, Viper) 6042.2330
e.g. for the connection from the TCC-3000RS (column outlet) to the DAD-3000RS,
MWD-3000RS, or VWD-3400RS
Viper capillary kit for Bio-RS systems with an UltiMate 3000 RS pump 6841.2301
Check valve cartridge, ceramics 6041.2301
(identical for inlet valve nut and outlet valve nut)
Check valve nut kit for double check valve, including
inlet check valve nut and outlet check valve nut
Stainless steel: RS pumps (S/N < 8030113) and SD pumps 6040.7007
Biocompatible: RS pumps (S/N ≥ 8030113), BM, and BX pumps 6042.7007
Cleaning swabs (e.g. for pump head connection ports) (pack of 10) 6040.0006
Degasser vacuum pump (LPG-3400) 6040.1981
Degassing chamber (analytical) (LPG-3400) 6030.9289
Detector (rear seal wash system) → Rear seal wash system, detector
Diagnostics Tool kit 6040.3099
The kit includes all material required for performing pump diagnostics tests from
Chromeleon, such as 1 fitting plug (Viper, biocompatible) and restrictor capillaries (2
capillaries with 15 m length each).
Display → Front panel door
Filter frit for solvent line filter for
SD pumps (stainless steel, porosity: 10 µm, 10 frits) 6268.0110
RS and BX pumps (titanium, biocompatible, porosity: 10 µm, 10 frits) 6268.0111
BM pumps (PEEK, porosity: 10 µm, 10 frits) 6268.0117
Filter frit with seals – to be used only with previous inline filters for ISO-3100 SD
(filter part no.6040.5151) and ISO-3100BM (filter part no. 6042.5016)
Frit (stainless steel, 10 µm) for previous 150 µL inline filter (part no. 6040.5151) in 6035.0016
ISO-3100SD
Frit (titanium, biocompatible, 0.2 µm) for previous 150 µL inline filter (part no. 6042.3335
6042.5016) in ISO-3100BM
Filter frit with seals for inline filter
Frit (biocompatible, 2 µm) for inline filter (10 µL) in LPG-3400BM/DGP-3600BM 6268.0036
and in the single-step mixing system for RS pumps (S/N ≥ 8030113)
Frit (biocompatible, 10 µm) for inline filter (10 µL) in the single-step mixing system 6268.0034
for RS pumps /(S/N < 8030113) and SD pumps (→ page 175)

Filter frit with seals – to be used only with the following previous static mixers:
For static mixer, mixing volume: 150 µL (part no. 6040.5151) 6035.0016
For static mixer, mixing volume: 350 µL (part no. 6040.5350) 6040.0040
The kit contains 2 filter frits (porosity: 10 µm) and 2 sets of seals.
Fitting plug (Viper, titanium, biocompatible) 6040.2303
Front panel door
Display 6820.0213
Front panel LED board 6820.0220
Fuses kit, including: 6030.9003
15 fuses (2A, slow-blow, 5 x 20 mm)
5 fuses (overload protection, 0.20A, slow-blow, 50 x 20 mm)
5 fuses (4A, slow-blow, 6.3 x 32 mm)
2 fuses (4A, slow-blow, 5 x 20 mm)
Note: Only use the slow-blow fuses rated at 2A (5 x 20 mm) with the pump.
Inline filter (with filter frit)
Inline filter (150 µL) for ISO-3100SD and ISO-3100BM 6040.5110
(replacing the previous inline filters 6040.5151 and 6042.5016)
Inline filter (10 µL) for LPG-3400BM and DGP-3600BM 6042.5014
Inline filter (volume: 10 µL) for use with the capillary mixer (volume: 25 µL) in the 6042.5014
single-step mixing system for RS pumps (S/N ≥ 8030113) (→ page 175)
Inline filter (10 µL) for use with capillary mixer (volume: 25 µL) in the single-step 6040.5010
mixing system for RS pumps /(S/N < 8030113) and SD pumps (except ISO-3100SD)
(→ page 175)
Inline filter, filter frits → Filter frits, inline filter
Installation tool for Viper capillaries with torque toothing →
Tool, installation tool for Viper capillaries
Leak sensor kit 6040.0009
Main board 6040.0201
Maintenance kit for DGP-3600BM—including: 6042.1952
6 solvent filters with filter frits
1.5 m silicone tubing (O.D. x I.D. 2.80 mm x 1.30 mm)
18 cm PharMed tubing (O.D. x I.D. 3.2 mm x 1.6 mm)
5 tubing connectors, straight, for I.D. 1.0-2.0 mm
4 piston seals (reversed phase), main piston seals
4 piston seals, seals in plate of seal wash system
4 support rings
2 O-ring seals (PTFE; 9x1.5 and 32x1.5) each for seal wash system
2 valve cartridges (ceramics)
2 filter frits (titanium, biocompatible, 2 µm)
2 cap seals for purge valve knob (only for knobs without integrated cap seal)
10 cleaning swabs

Maintenance kit for DGP-3600RS—including: 6040.1955A

10 cleaning swabs
Maintenance kit for DGP-3600SD—including: 6040.1952
4 support rings (only for pumps with max. working pressure ≤ 50 MPa)
10 cleaning swabs
Maintenance kit for HPG-3x00RS—including: 6040.1956B
4 solvent filters with filter frit
1 tubing connector, 90°-angled
3 tubing clips (self-adhesive)
1 cap seal for purge valve knob (only for knobs without integrated cap seal)
5 cleaning swabs
Maintenance kit for HPG-3x00SD—including: 6040.1953
10 cleaning swabs

Maintenance kit for ISO-3100BM—including: 6042.1950

1 solvent filter with filter frits
2 support rings
1 O-ring seal (PTFE; 9x1.5 and 32x1.5 each) for seal wash system
1 valve cartridge (ceramics)
5 cleaning swabs
Maintenance kit for ISO-3100SD—including: 6040.1950
1 solvent filter with filter frit
2 piston seals, seals in plate of the seal wash system
1 O-ring seal (PTFE; 9x1.5 and 32x1.5) each for seal wash system
5 cleaning swabs
Maintenance kit for LPG-3400BM—including: 6042.1951
2 support rings
1 filter frit (titanium, biocompatible, 2 µm) for inline filter
5 cleaning swabs
Maintenance kit for LPG-3400RS—including: 6040.1954A
1 tubing connector, 90°-angled
2 tubing clips (self-adhesive)
5 cleaning swabs

Maintenance kit for LPG-3400SD—including: 6040.1951

5 cleaning swabs
Maintenance kit, NP, for HPG-3200BX—including: 6042.1954
2 solvent supply line filters with filter frits
1.5 m PharMed tubing (O.D. x I.D. 3.2 mm x 1.6 mm)
4 piston seals (normal phase)
4 support rings
2 O-ring seals (PTFE; 9x1.5 and 36.5x1.5) each for seal wash system
10 cleaning swabs
Maintenance kit, RP, for HPG-3200BX—including: 6042.1953
2 solvent supply line filters with filter frits
4 piston seals (reversed phase)
4 support rings
2 O-ring seals (PTFE; 9x1.5 and 36.5x1.5) each for seal wash system
10 cleaning swabs
Mixer, capillary mixer, Viper, MP35M for RS pumps (≥ S/N 8030113)
Capillary mixer (volume: 25 µL) to be used with: 6042.3020
- Static mixer (volume: 75 µL) in the two-step mixing system (100 µL)
- Inline filter (volume: 10 µL, part no. 6042.5014) in the single-step mixing system
Capillary mixer (volume: 50 µL)1 for
LPG-3400RS 6042.3026
DGP-3600RS 6042.3025
HPG-3x00RS 6042.3015
1
To be used in two-step mixing systems with mixing volumes > 100 µL.
Mixer, static mixer for HPG-3200BX
Static mixer (volume: 750 µL) 6040.5750
Mixer, static mixer, filter frit → Filter frit, static mixer

Mixers, capillary mixers, stainless steel—for RS pumps (S/N < 8030113) and
SD pumps (except ISO-3100SD)
Capillary mixer (volume: 25 µL) 6040.3020
to be used together with:
- static mixer (volume: 75 µL) in the two-step mixing system (volume: 100 µL)
- inline filter (volume:10 µL, part no. 6040.5010) in the single-step mixing system
Capillary mixer (volume: 50 µL)* for
LPG-3400SD 6040.3026
DGP-3600SD 6040.3025
HPG-3x00SD 6040.3015
* To be used in the two-step mixing system with a mixing volume > 100 µL.
Mixers, static mixers — RS and SD pumps (except ISO-3100SD)
Static mixer (volume: 75 µL)1 (RS pumps ((S/N < 8030113), SD pumps) 6040.5100
Static mixer (volume: 75 µL)1 (RS pumps ≥ S/N 8030113) 6042.5100
Static mixer (volume: 150 µL)2 (replacing the previous a static mixer 6040.5151) 6040.5110
Static mixer (volume: 350 µL)2 (replacing the previous static mixer 6040.5350) 6040.5310
Static mixer (volume: 750 µL)2 6040.5750
Static mixer (volume: 1500 µL)2 6040.5450
1
The mixer is used together with an appropriate capillary mixer (volume: 25 µL) in
the two-step mixing system (mixing volume: 100 µL). The capillary mixer is included
in the shipment.
2
These static mixers are to be used with a capillary mixer (volume: 50 µL). The
capillary mixer is installed when the pump is shipped, and thus it is not included in the
shipment.
Mixing system, stainless steel, (volume: 35 µL), single-step for 6040.5000
RS pumps (S/N< 8030113) and SD pumps (except for ISO-3100SD), including
capillary mixer (volume: 25 µL) and
inline filter (volume: 10 µL)
Mixing system, titanium/MP35N (volume: 35 µL), single-step 6042.5000
for RS pumps (≥ S/N 8030113), including
Capillary mixer, Viper, MP35N (volume: 25 µL) and
Inline filter, titanium (volume: 10 µL)
Normal Phase (NP) Kit for HPG-3200BX, including: 6040.1975
PharMed tubing (1 m, O.D. x I.D., 3.20 x 1.60 mm)*
Detector (NP) for rear seal wash system
1 tubing connector, straight, for I.D. 1.0 - 2.0 mm
8 piston seals, normal phase (→ Fig. 32, page 55, nos. 3 and 7)
4 support rings
* If you observe problems with special NP applications, consider replacing the silicone
tubing and detector of the seal wash system with the PharMed tubing and
NP detector from this kit.

Normal Phase (NP) Kit for SD(N) pumps, including: 6040.1972

PharMed tubing (1 m, O.D. x I.D., 3.20 x 1.60 mm)*
Detector (NP) for rear seal wash system
1 tubing connector, straight, for I.D. 1.0 - 2.0 mm
8 piston seals, normal phase (→ Fig. 31, page 51, nos. 3 and 7)
4 support rings (only for 50 MPa SD pump versions)
* If you observe problems with special NP applications, consider replacing the silicone
tubing and detector of the seal wash system with the PharMed tubing and
NP detector from this kit. Replacing these components is required also for running NP
applications with SDN pumps.
Performance Qualification kit – Contact your local support or sales organization. --
Peristaltic pump 6040.1150
Piston
All pumps except HPG-3200BX (sapphire, 2 pistons) 6040.0042
HPG-3200BX (ceramics, 2 pistons) 6040.0842
Piston seal (in plate of seal wash system) for
RS, SD, and BM pumps (2 seals) 6040.0033
SDN pumps (2 seals) 6040.0306
HPG-3200BX (kit including 2 seals and 1 support ring) 6040.9010
Piston seal (main piston seal) for
SD pumps
Reversed Phase (2 seals) 6040.0304
Normal Phase (2 seals) 6040.0306
SDN pumps
Normal Phase (2 seals) 6040.0306
Piston seal (main piston seal) for RS pumps
Reversed Phase (2 seals) 6266.0305
Piston seal (main piston seal) for BM pumps: 6025.2012
Reversed Phase (kit including 2 seals and 1 support ring)
Piston seal (main piston seal) for HPG-3200BX:
Reversed Phase (kit including 2 seals and 1 support ring) 6040.9010
Normal Phase (kit including 2 seals and 1 support ring) 6040.9011
Proportioning valve
3-channel (for DGP-3600) 6040.4026
4-channel (for LPG-3400) 6040.4025
Pulse damper for pumps with serial numbers:
Up to 8086057 6042.6000
8086058 and higher 6042.6010
Pulse damper maintenance kit for pumps with serial numbers:
Up to 8086057 6042.6001
8086058 and higher 6042.6011

Pump block (without fluidics) for

RS pumps 6040.5020
SD pumps 6040.5030
BM pumps 6040.5040
HPG-3200BX 6040.5050
Pump head (entire assembly) for
HPG-3x00RS 6040.1901B1
HPG-3x00SD 6040.1903A2
HPG-3200BX 6042.1901
ISO-3100SD, LPG-3400SD, and DGP-3600SD 3
6040.1904A
LPG-3400RS and DGP-3600RS 6040.1902B 1
ISO-3100BM, LPG-3400BM and DGP-3600BM 6041.1902

Notes:
1
The part number extended by the 'B' replaces all previous part numbers (that is,
those extended by an ‘A’ or without letter extension). The 'B' pump head is fully
backward compatible. You can use the 'B' pump head with all versions of the related
pump type. Use pump heads without an 'A' or ‘B’ in the part number only with the
80 MPa pumps.
2
The part number extended by the 'A' replaces the part number without the 'A'. The
'A' pump head is fully backward compatible. You can use the 'A' pump head with all
versions of the related pump type. Use pump heads without an 'A' in the part number
3
This pump head has reversed phase seals installed as main piston seals. You can use
the pump head also with SDN pumps. However, in this case, consider replacing the
reversed phase seals with normal phase seals.
Purge unit for
HPG-3x00SD 6040.7155A*
HPG-3x00RS and HPG-3200BX 6042.7157A
ISO-3100SD, LPG-3400SD, and DGP-3600SD 6040.7165A*
ISO-3100BM, LPG-3400(RS,BM), and DGP-3600(RS, BM) 6042.7155A*
* Note:
The part numbers extended by an 'A' replace the part numbers without the 'A'. The 'A'
purge units are fully backward compatible. You can use them with all versions of the
related pump type. Use purge units without an 'A' in the part number only with the 50
MPa or 80 MPa pump versions.
Purge valve knob (including seals)—all pump types 6040.2035
Note:
Part no. 6040.2035 replaces part no. 6040.2030—the design of the purge valve knob
has changed. The new knob has an integrated cap seal, which is not subject to wear.
The knob is fully backward compatible and can be used in all purge units. (However,
the earlier knob version (part no. 6040.2030) must be used only with the 50 MPa or 80
MPa pump versions.)
Purge valve knob, cap seal assembly tool → Cap seal assembly tool
Purge valve knob, cap seal → Cap seal
Rear seal wash system, detector 6040.4131
For NP applications, see the note on page 77.

Rear seal wash system, seals 6040.2208

(→ nos. 4 and 8 in Fig. 31, page 51; no. 4 in Fig. 32, page 55)
5 PTFE seals 9x1.5 und 32x1.5
Rear seal wash system, tubing kit, including: 6040.9502
silicone tubing (1.5 m, O.D. x I.D. 2.80 x 1.30 mm)
PharMed tubing (18 cm, O.D. x I.D., 1.60 x 3.20 mm)
7 tubing connectors, straight, for tubing I.D. 1.0 - 2.0 mm
Seal handling tool (for piston seal removal and insertion) 6040.7158
Shut off valve for solvent supply line (HPG-3200BX), set including 6042.2531
1 shut off valve for solvent supply line
1 tubing adapter (1/8")
Signal cable (6-pin, mini-DIN) 6000.1004
to connect external devices to the digital I/O ports on the pump
Solvent line filter
Filter holder (6 holders), all pump types except HPG-3200BX 6268.0115
Filter holder (2 holders), HPG-3200BX 6268.0116
Filter frit (stainless steel, porosity: 10 µm, 10 frits),SD pumps 6268.0110
Filter frit (titanium, biocompatible, porosity: 10 µm, 10 frits), RS and BX pumps 6268.0111
Filter frit (PEEK, porosity: 10 µm, 10 frits), BM pumps 6268.0117
Solvent selector "2 from 4" (HPG-3400RS) 6040.2611
Solvent supply line (O.D. 3.0 x I.D. 1.5 mm) with fittings 6030.2542
Solvent supply line from degasser to proportioning valve, DGP-3600 6030.2547
(Pack of 3 solvent supply lines, fitting connections, and line labels)
Solvent supply line from degasser to pump head, HPG-3200 6035.2530
Solvent supply line from degasser to pump head, ISO-3100 6030.2546
(Pack of 1 solvent supply line, fitting connections, and line label)
Solvent supply line from degasser to solvent selector , HPG-3400 6035.2532
Solvent supply line to the LPG-3400 degasser 6040.2049
Solvent supply line, HPG-3200BX 6042.2530
(Pack of 2 solvent supply lines with adapter for the connection to inlet valve)
to connect the solvent reservoirs directly to the pump
Solvent supply line, ISO-3100BM 6030.2548
for connecting the solvent reservoir directly to the pump
Static mixer → Mixer, static mixers
Support ring for piston seal (2 support rings) for SD, RS, and BM pumps 6040.0012
Support ring/piston seal kit, normal phase, for HPG-3200BX, including 6040.9011
2 piston seals (normal phase) and 1 support ring
Support ring/piston seal kit, reversed phase, for HPG-3200BX, including 6040.9010
2 piston seals (reversed phase) and 1 support ring

Switching power supply (24V/150W) 6820.2820

Tool kit for analytical UltiMate 3000 HPG-3200BX, including: 6007.9306
1 open-end wrench (1/4" x 5/16")
1 open-end wrench (11 x 13 mm)
1 open-end wrenches (17 x 19 mm)
1 hexagon wrench (size 6 mm)
1 piston seal removal and insertion tool
1 pump head tool (required or establishing the distance during piston installation)
Tool kit for analytical UltiMate 3000 RS, SD, and BM pumps, including: 6007.9304
1 open-end wrench (1/4" x 5/16")
2 open-end wrenches (11 x 13 mm)
1 hexagon wrench (size 6 mm)
1 piston seal removal and insertion tool
1 pump head tool for RS and SD pumps and
1 pump head tool for BM pumps and NCS-3500RS/NCP-3200RS
(The pump head tool is required or establishing the distance during piston installation.)
Tool, installation tool for Viper capillaries with torque toothing 6040.2314
Top cover (with front panel door and display) for
RS pumps 6040.1800
SD, BM, and BX pumps 6040.1810
Tubing from degasser to proportioning valve, LPG-3400 6040.2540
(pack of 4 tubes with fitting connections)
Tubing from proportioning valve to pump head for
LPG-3400SD/RS and DGP-3600SD/RS 6040.3023
LPG-3400BM and DGP-3600BM 6042.3023
(Pack of 2 tubes with fitting connections)
Tubing from solvent selector to pump head (pack of 2), HPG-3400 6040.3017
Tubing kit, rear seal wash system → Rear seal wash system, tubing kit
Upgrade kits to upgrade 50 MPa SD pumps to 62 MPa
ISO-3100SD and LPG-3400SD 6040.0011
HPG-3x00SD and DGP-3600SD 6040.0021
Valve cartridge, check valve → Check valve, valve cartridge
Valve nut kit for check valve → Check valve, valve nut kit

13 Appendix
13.1 Digital I/O (Pin Assignment)
The two digital I/O ports provide two digital inputs and two relay outputs that can be used
to exchange digital signals with external devices.
* NO = normally open contact

NC = normally closed contact
Relay_NO* Relay_COM COM is the common contact for NO and NC. If
the relay is not activated or if the pump is turned
Input Ground
off, the connection is between COM and NC. If
Relay_NC* Not used the relay is activated, the connection is between
COM and NO.
Fig. 116: Mini-DIN Digital I/O port
To connect an external device to the digital I/O ports, use the appropriate mini-DIN cable
(part no. 6000.1004). The table lists the functions assigned to the connector pins and the color
and label of the cable wire connected to each pin (ignore the labels on the cable itself).
Pin Wire color Signal Name Signal Level Note
1 Pink Not used

2 Grey Relay_NC Potential free Normally closed contact
(functionality available with main board
revision B and later)
3 Green GND Ground Reference potential
4 Yellow Input TTL Digital input
5 Brown Relay_COM Potential free Common contact for NO and NC
6 White Relay_NO Potential free Normally open contact
Fig. 117: Pin assignment (port and cable)
Important: The maximum switching voltage of the relays is 24 V. The switching

current must not exceed 100 mA.
The maximum input voltage at the input must not exceed +5 V with
reference to ground. The minimum input voltage must not be lower than
the ground potential.
Tip: The input has a pull-up resistor.
If you want to connect a Corona or Coulochem III detector to the pump, refer to the
Chromeleon Help for details. The Help provides separate sections for connecting the two
detectors to an UltiMate 3000 system pump, including details about the appropriate cabling
and the settings required in the Server Configuration program.

13.2 Solvent Rack (Pin Assignment)
Pin Signal Name Signal Level Remarks
1 Reserved
2 Solvent Rack Error TTL_high with Solvent Rack errors
3 Jumper to pin 9
4 Solvent Rack Leak TTL_high with Solvent Rack leaks
5 Reserved
6 V_Degas +15V_supply Supply for the Solvent Rack
7 GND_Degas Ground_supply Reference potential for V_Degas
8 VCC Voltage for logic devices
9 Jumper to pin 3
10 GND Reference potential for VCC
13 Reserved
14 V_Degas +15V_supply Supply for the Solvent Rack
15 GND_Degas Ground_supply Reference potential for V_Degas
Fig. 118: 15-pol. Solvent Rack port (female)

UltiMate 3000 Series: 2G Pumps
Troubleshooting Guide—
Recommendations for Leakage-Free Pump Operation
Troubleshooting— Recommendations for Leakage-Free Pump Operation
This document provides information about possible causes for pump leakage that have been
observed at some customer sites in the past. Most of the information listed here is also
provided in the related sections of the service manual.
The steps below help you to identify and eliminate the source for pump leakage and lead to
successful troubleshooting. For an overview of how to proceed if one of the below issues was
found, also refer to the flow charts that are appended to this document.
Troubleshooting Steps
1. "Pump leakage" is a general description for a range of different issues. Choose which of
the following issues was observed:
• Liquid can be observed the between the pump head and the rear seal wash system plate,
on capillary connections, or valve nuts. Continue reading step 2.
• The data shows pressure pulsation. In this case, check if the rear seal leakage counter
(Service Level) has counted drops. If yes, continue reading step 4. If the answer is no,
exchange the check valve cartridges.
• The following message is shown on the display or in the Chromeleon Audit Trail: “The
piston seal leakage has exceeded the recommended limit.” Check if there are real drops
visible on the rear seal wash system. If the anwer is yes, continue reading step 4. If the
answer is no, this indicates that the liquid in the rear seal wash system was not caused
by defective seals or pistons. Continue reading step 6.
• The following message is shown on the display or in the Chromeleon Audit Trail: “The
rear seal leak sensor detects drops constantly.” Check if water has accumulated in the
detector of the rear seal wash system. If the answer is yes, the waste line of the rear seal
wash system, which is routed under the pump to the drain port of the pump, may be
clogged. If the answer is no, continue reading step 6.
2. Check if the valve nuts and the fittings to the valve nuts are tight. Tighten any loose
connections if necessary. If the thread of a valve nut is damaged, replace the valve nut.
Also check the threads inside the pump head for damage. If there are scratches, you may
have to replace the pump head.
Check if the white seal rings (see Fig. 1) in the rear seal wash system are in good condition.
If either seal ring appears to be damaged or squeezed, replace both rings.
Seal rings
Fig. 1: Seal rings of the rear seal wash system

If the fittings, valve nuts, and rings are in order, continue reading step 3.
Rev. 1.0, April 2013 Page 1

3. Inspect the tubing and connections of the rear seal wash system for indications of leakage
or damage. Tighten or replace leaking connections or tubing as necessary.
4. (DPG and HPG only): Inspect both pump blocks to determine which block is affected.
a) Disconnect the rear-seal wash tubing from the pump head.
b) During normal pump operation, visually inspect the rear-seal wash tubing connection
ports of the pump heads for liquid leaks. If leakage is low, for example, less than 4 drops
per hour, it may take some time until liquid appears at the connection ports.
Replace the piston seals and/or clean or replace the piston in the affected pump block as
described in step 5.
5. Replace the piston seals and/or clean or replace the piston if necessary. To do so, follow
the instructions in the service manual. Keep in mind that piston and seals are working
together while they are sealing. Therefore:
• Inspect the piston for contamination (such as, deposits, coatings, or films) or damage
(such as scratches) by using a light, and/or by using the fingernail test if necessary.
Piston damage is one of the major root causes for repeated leakage.
• Observe the installation instruction in the service manual. Always undock the pistons
before removing the pump head.
• When you remove the seals, be careful not to scratch the inside of the pump head.
Scratches inside the pump head prevent proper sealing. A visible scratch will result in
pump leakage. To remove the seals properly, always use the seal removal tool.
• Use the seal installation tool to insert the new seals.
• Use the pump head tool (spacing tool) to establish the correct distance between the
piston and the plate of the rear seal wash system (the accessories kit for the pump
includes a pump head tool that is appropriate for this pump type).
• Don’t dock the pistons before you have secured the pump head firmly in place with the
screw.
• After exchanging parts, thoroughly clean and flush the entire fluidic system of the pump
(such as pump heads, valves, and capillaries) and the rear-seal wash system. Remaining
contaminants may be responsible for repeated leakage even after repair.
• Did you run the pump diagnostic tests (in CM 6.80; not available for BX pump)?
• To achieve optimal seal performance, allow new seals to run in. Operate the pump for
about 30 minutes at 30 to 40 MPa with, for example, isopropanol, and an appropriate
backpressure loop. You may have to repeat the diagnostic tests a couple of times to
achieve a pass result.
• If leakage continues after your repair, leakage may caused by a part that has not yet
been replaced. If you replaced, for example, the seals, consider replacing also the
pistons and/or pump head.
• If possible, consider returning the complete pump head with the seals still mounted to
the factory for further examination.

6. Remove the detector of the rear seal wash system to discover if liquid has accumulated
under the detector. If this is the case, the waste line of the rear seal wash system, which is
routed under the pump the drain port of the pump, may be clogged. If no liquid has
accumulated, continue reading step 7.
7. Inspect the detector of the rear seal wash system for signs of corrosion or deposits. If
there are signs of corrosion or deposits, replace the detector of the rear seal wash system.
If not, continue reading step 8.
8. Inspect the electrodes of the rear seal wash detector: Is there a drop hanging under either
of the two electrodes? If yes, clean the detector electrodes. If no, check if there is air in
the rear seal wash system and continue reading step 3. Another reason for the leakage
may be that the RP detector is used for NP applications. Verify that the correct detector is
used. Install a different detector if necessary as described in the service manual.
9. If you are unable to eliminate the problem by following the instructions given here,
contact Support for Dionex HPLC Products. In this case, a systematical problem with the
application may be the root cause. Consider the following questions and actions:
• User errors or poor quality of the used solvents and chemicals are often the reason for failures.
♦ Check for the sources and the quality of solvents and chemicals.
Consider evaporating some solvent in a clean glass and check the glass for
contamination.
♦ Try a different batch of solvents and chemicals.
♦ Use solvents and chemicals of one or more different suppliers.
♦ Verify that aqueous eluent solutions are freshly prepared. They should not be older
than 2 or 3 days. This is especially important when they contain phosphate or
nitrate buffer salts.
♦ Might the in-house water supply of the customer be defective or contaminated?
Has it been maintained properly?
♦ Sometimes, users do not realize solvent compatibility issues with NP (normal
phase) and RP (reversed phase) solvents. Check whether the installed seals are
compatible with ALL solvents (analysis, flushing, etc.) in use.
♦ Verify that the solvents are not recycled. With solvent recycling, small pump
abrasion particles may be circulated as well, leading to seal deterioration, and thus
impairing the performance of the seals.
♦ Does the customer use eluent filter frits? If yes, which ones are used? If the frits
were removed for cleaning, have they been re-installed correctly? Re-installation of
a frit the other way round allows the transfer of small particles into the pump heads
and hence may cause seal damage.
♦ Have tubings or filter frits been changed that do not comply with the recommended
system setup? Did the customer perform these potential changes, probably without
letting you know?
♦ Is there a frequent change of users and applications on the respective system?

• If methanol is used as HPLC solvent

♦ Does it come from aluminum reservoirs?
♦ Are the eluent bottles made of glass, aluminum, or even of a different material?
♦ With eluent bottles made of glass: Did the user fill the glass bottles with methanol
that was previously stored in an aluminum reservoir?
Using methanol from aluminum reservoirs may cause deposits on the pistons and thus
impair the performance of the seals. This effect depends on the quality of the aluminum
reservoirs; therefore, it may not always be observed.
• In most cases, an increased rear-seal leak count does not affect chromatographic performance
(verify % RSD of peak retention times and areas). In this case, you may consider increasing
the value for the rear-seal leak limit (RearSealLeakage → Limit) in Chromeleon if the
customer agrees.
• Reduce the organic content (methanol or isopropanol) in the rear-seal wash reservoir to 5 to
10% organics, the recommended solution is standard HPLC grade water with 10% methanol.
• LPG pumps only
♦ Eluents may not be miscible at certain concentration levels. Example:
Mobile phase A is a 50 mmol/L phosphate buffer.
Mobile phase B is 100% acetonitrile.
If you run a gradient from 10 % B to 90% B, the buffer salt may precipitate and
ruin the seals. Check the miscibility of the eluents by preparing the appropriate
mixtures with the highest concentration of organic solvents in a graduated cylinder.
♦ After the end of a sample batch, check whether the pump is still running at a very
low flow rate (for example, 0.050 mL/min or less) in mixing mode with a buffer
solution in channel A and an organic solvent in channel B. With these conditions,
we occasionally observed salt precipitation and hence damaged proportioning
valves in the past. Salt precipitate can enter the pump head and ruin the seals.
10. When contacting Support for Dionex HPLC Products, observe the following:
• If you replaced pistons and seals, keep the corresponding pairs together. Label them properly
and return them to the factory for further detailed analysis. Let us also know how many drops
per hour the rear seal leakage counter (Service Level) indicates for the seals that you return to
the factory.
• Do not forget to indicate a valid addressee for your shipment.
• If you are reporting repeated leakage, provide a detailed and accurate history of your actions
and observations.
• Provide raw data (cmb files, daily audit trails, System Status Reports) as early as possible.
Also, make sure to record the service channels as described in the Service Instructions under
"Recording the Service Channels/Contacting Support". This will facilitate and thus speed-up
the data evaluation and therefore help to de-escalate the situation at the customer site at an
early stage.

A Liquid can be observed
on rear seal wash system
(RWS) or connections
valve nut
Yes loose? No
tighten the
valve nut valve nut
Yes thread No
damaged?
replace the
valve nut or
seal rings
pump head
Yes damaged? No
replace the
seal rings RWS 1
Yes tubing No
leaking?
replace the
RWS tubing RWS con-
Yes nections No
leaking?
replace the contact
RWS Support
connections
B The data shows
pressure pulsation
Message: "The piston seal
leakage has exceeded the
recommended limit."
leakage real drops

No counter counts visible on
drops? Yes Yes
RWS?
replace the
continue with
check valve determine which flow chart C
cartridges pump block is affected
replace piston seals;

clean or replace piston
C
Message: "The rear seal
leak sensor drops
constantly"
water in
Yes the RWS No
detector
unclog RWS
waste line
corrosion
or deposits
Yes No
on RWD
detector?
replace
RWS
detector drop hanging
on detector
Yes No
electrode?
clean
electrodes air in
Yes RWS? No
continue as
described in flow
RP detec-
chart A position 1 Yes No
1 tor used
for NP?
Install an contact
NP detector Support

Ultimate 3000 2g Pumps Service Instructions v1-6

Uploaded by

Document Information

Original Title

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

Ultimate 3000 2g Pumps Service Instructions v1-6

Uploaded by

Copyright:

Available Formats

Thermo Scientific

UltiMate 3000 Series

© 2017 Thermo Fisher Scientific

2 Preparatory Measures .................................................................................................. 5

3 General Pump Design ................................................................................................... 8

4 Instrument Checkout .................................................................................................. 14

5 Chromeleon Service Functions .................................................................................. 18

6 Maintenance and Service ........................................................................................... 28

Service Instructions Thermo Fisher Scientific Confidential Page i

6.6 Top Cover ................................................................................................................ 42

Service Instructions Thermo Fisher Scientific Confidential Page ii

7 Troubleshooting ........................................................................................................ 124

8 Updating the Pump Firmware ................................................................................. 154

9 Interior Components and Flow Path ...................................................................... 156

10 Optimizing the Pump for Specific Applications..................................................... 173

11 Connecting a Corona or Colouchem III Detector.................................................. 177

12 Consumables and Spare Parts ................................................................................. 178

13 Appendix .................................................................................................................... 191

Service Instructions Thermo Fisher Scientific Confidential Page iii

The following conventions apply to the descriptions throughout this manual:

Service Instructions Thermo Fisher Scientific Confidential Page 1

Tip: Indicates general information intended to optimize the service and

The information contained in this document is subject to change without notice.

Service Instructions Thermo Fisher Scientific Confidential Page 2

1.2 Additional Documents

Service Instructions Thermo Fisher Scientific Confidential Page 3

1.3 Instructions for Returning Instruments or Parts

Warning: Possible Contamination

Service Instructions Thermo Fisher Scientific Confidential Page 4

Observe the following general precautions:

Service Instructions Thermo Fisher Scientific Confidential Page 5

2.2 Safety Precautions

Important: To avoid electrostatic discharge, which may result in damage to

Service Instructions Thermo Fisher Scientific Confidential Page 6

2.3 Precautions for Connecting and Operating an ISO-3100BM

When operating an ISO-3100BM, observe the following:

Service Instructions Thermo Fisher Scientific Confidential Page 7

3 General Pump Design

Serial Number Modifications Remarks

8034523 Main board revision B Extended Digital I/O functionality

Service Instructions Thermo Fisher Scientific Confidential Page 8

Upgrade Kit Part No.

To upgrade SD pumps to 62 MPa

Service Instructions Thermo Fisher Scientific Confidential Page 9

3.2 Rear Panel

Fig. 1: Rear panel view

Service Instructions Thermo Fisher Scientific Confidential Page 10

3.3 Power Connection

3.4 Fuse Cartridge (Main Power Fuses)

3.5 Interior Components and Liquid Flow Path

For the … Find the … On page

ISO-3100SD Interior components 157

Service Instructions Thermo Fisher Scientific Confidential Page 11

3.6 View into the Enclosure

Service Instructions Thermo Fisher Scientific Confidential Page 12

Fig. 3: Interior view—here fan installed in an HPG-3400RS

Service Instructions Thermo Fisher Scientific Confidential Page 13

4.1 Main Board

Fig. 4: Main board (here revision A)

No. Function Remark

Service Instructions Thermo Fisher Scientific Confidential Page 14

No. Function Remark

8 Connectors for the degasser vacuum With LPG-3400 pumps only