Chapter 5 Summary And Conclusion

The antibacterial potential of Beta vulgaris and Mentha was explored in the present study, and
it was observed that extract the maximum antibacterial activity for E. coli strain was exhibited
by 1:2 extract at 30μL, it shows the 14mm diameter zone of inhibition. Lowest activity shown
by tol extract at 10 μL, it shows the 12mm diameter zone of inhibition. In S. aureus maximum
activity was exhibited by DMSO extract at 30 μL, it shows the 18mm diameter zone of
inhibition. Lowest activity shown by 1:1 extract at 50 μL, it shows the 12mm diameter zone of
inbition. In sonication extract, the maximum antibacterial activity for E. coli strain was
exhibited by 2:1 extract at 30μL, it shows the 16mm diameter zone of inhibition. Lowest
activity shown by t extract at 10 μL, it shows the 14mm diameter zone of inhibition. InB.
subtilis maximum activity was exhibited by DMSO extract at 70 μL, it shows the 27mm
diameter zone of inhibition. Lowest activity shown by 2:1 extract at 50 μL, it shows the 16mm
diameter zone of inhibition.
According to the results of the present study and since Mentha Leaf is a cost-effective,
available, and useful plant in medicine, it can be used as the best choice for the biosynthesis of
nanoparticles. Among the methods of nanoparticles synthesis, biosynthesis is the cost effective,
safe, and bio-compatible one. Since in this method, silver nanoparticles are synthesized without
using hazardous chemicals, they can be used in the health-related industries such as healthcare
and the environment. This study showed the successful synthesis of silver nanoparticles using
the extract of Mentha L. This method is a cost-effective and rapid method that can be applied
at room temperature. The result of the studied showed that World News of Natural Sciences
18(2) (2018) 163-170 -169- the green synthesized silver nanoparticles possessed to have
significant antimicrobial properties. Thus, the green synthesized silver nanoparticles from Mint
can be used as a curative agent for targeted drug delivery to cure diseases. This may be due to
the mode of action of silver ions against the bacteria. These silver ions can cause a destruction
of peptidoglycan cell wall and lysis of cell membrane. The silver ions bind to DNA bases,
causes, and condense the DNA to lose its ability to replicate, thereby prevents replication via
binary fission. We found out a simple green protocol for synthesizing and surveying
antibacterial properties and catalytic activity of the silver NPs from the Beetroot extract. Ag
NPs were S. Mehdizadeh et al. / Eurasian Chemical Communications, 2019, 545-558 Page |
556 synthesized using Beetroot extract at optimum conditions. The NPs were characterized by
UV-Vis, FTIR, XRD, FESEM and TEM techniques. The UVVis analysis determined the
Surface Plasmon Resonance of the AgNPs. The FT-IR measurements were applied to identify
the potential of functional NPs that can be used as beneficial and practical catalysts. The FE-
SEM, TEM, and XRD techniques confirmed the formation of cubi crystalline structure. NPs

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Chapter 5 Summary And Conclusion

have improved catalytic activity, because larger surface area and smaller size are considered as
important features in nanoparticles. The catalyst is eco-friendly and stable, because it produces
negligible waste without significant loss of activity and with nearly complete conversion.
However, the mechanisms behind the activities of NPs need to be precisely studied in detail.
The functional aspect of the silver NPs in terms of their surface crystallization, stability and
size distribution was significantly acquired. Overall, the synthesized silver NPs bring a
valuable opportunity for controlling and inhibiting the growth of some pathogenic and
dangerous bacteria.
Various nutrients are found in beetroot’s leaves and roots, including carbohydrate, fibers,
limited protein, minerals (iron, magnesium, zinc, selenium, potassium, calcium, copper,
phosphorus, sodium), vitamins (A, B3, B7, C, B6, B9), betaine, betaines, and phenolic
compounds such as flavonoids and saponins, caffeic acid, epicatechin, and rutin.
Betaine is an amino acid (trimethyl-glycine) derivative of the glycine naturally found in
animals, microorganisms, and plants. It was first discovered in the extraction of sucrose from
beetroot and was later found in other foodstuffs, including wheat products, shrimp, and spinach.
Betaine can also be found in many tissues, derived from endogenous synthesis from choline,
principally in the mitochondria of the liver and kidneys.
The increasing demand for mint essential oil in the cosmetic and personal care industry will
bring huge development opportunities for the mint essential oil market. The usage in cosmetic
products is like that of pharmaceuticals. This serves special quality such as high temperature
resistance in tropical regions or sun protection. Furthermore, the rising urbanization land the
increasing disposable income of various individuals over all the globe which have led to a rise
in the sales of personal and cosmetics care products. Hence, these factors will significantly
shape the growth of the mint essential oil market.

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