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Marker Assisted Selection in The Honey Bee
Marker Assisted Selection in The Honey Bee
What is marker-assisted selection? How do you find markers for marker-assisted selection? How do you use the markers for breeding?
•The honey bee genome sequencing •Laboratory tests identify the
Susceptible
Susceptible
Susceptible
Resistant
Resistant
Resistant
Using unique genomic sequences that associate with a trait of project1 identified millions of unique genomic sequence by amplifying the
interest to chose which individuals to breed in order to keep the genomic patterns that can be used as DNA surrounding the marker, then
trait in the next generation. “markers” for mapping of traits to the treating the amplified DNA with
genome. special enzymes that differentiate
DNA from disease resistant bee which pattern each bee has.
•Develop a backcrossed population of bees
Choose breeding stock with that show variability in the trait. •Potential breeding stock is evaluated
Drone source 1
Drone source 2
Drone source 3
Drone source 4
Drone source 5
Drone source 6
this marker pattern for the marker in a lab.
Avoid breeding stock with •Screen each bee for the trait then screen
DNA from susceptible bee this marker pattern markers throughout the genome to find •Compatible stocks are bred by
which markers associate with the trait. instrumental insemination for tightly
Those that associate are in or near the gene controlled traits, or by natural mating
that controls the trait. for less stringent breeding.
Ars.usda.gov
•What is Chalkbrood resistance? •What is tracheal mite resistance?
Resistance that is observed in inoculated honey bee larvae where the Resistance that is observed in adult honey bees where either through
resistant individuals survive the infection whereas the susceptible larvae physiological or behavioral mechanisms the infestation of tracheal mites is
form the classical chalkbrood disease symptoms of fungal hyphae reduced or inhibited.
•What is VSH?
formation at the posterior.
A behavior exhibited by adult bees where capped brood that are infested
by Varroa are preferentially uncapped, inspected, cleaned or removed. •Current status of mapping
•Current status of mapping Quantitative trait loci mapping has found a region on chromosome 7 that
Quantitative trait loci mapping has found a region on chromosome 11 that has moderate control over the trait.
•Current status of mapping
has strong control over the trait. Fine mapping has narrowed the region
Quantitative trait loci mapping has found a region on chromosome 9 that 3
on chromosome 9 to an interval of 36kb containing 2 genes or gene *
has strong control over the behavior, and a region on chromosome 1 that 2.5
segments 3.
has minor control 2. Fine mapping has narrowed the region on 2
LOD score
chromosome 9 to an interval of 89kb containing segments of 2 genes. 1.5
AMB-00858654
AMB-00612262
1
AMB--00002515
AMB-00102059
0.5
0
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23
Position
36Kb
89Kb
NM_003377999 NM_003378155
5’ 5’
JHBP-like 5’ SIGIRR 5’ •Markers for the interval
Basement membrane-specific D2-like dopamine receptor
Markers were evaluated and found to not validate in a related and
heparan sulfate proteoglycan core
protein
selected population 4.
resistance of colonies and individual honey bees. Submitted to Journal of Apicultural Research