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Biotechnology and its Applications

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Synopsis
Major Applications of Biotechnology

Biotechnological Applications in Agriculture

Biotechnological Applications in Medicine

Transgenic Animals & Ethical Issues


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Recap
Applications of Biotechnology
The applications of biotechnology include
Therapeutics
Diagnostics
Genetically modified crops
Processed food
Bioremediation
Waste treatment
Energy production
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!!
Three critical research areas of biotechnology are:
Providing the best catalyst in the form of improved organism
usually a microbe or pure enzyme.
Creating optimal conditions through engineering for a catalyst to
act
Downstream processing technologies to purify the protein/organic
compound
Bt Plants

Cry gene
=
Crystal protein
Bt Cotton Bt Brinjal
Bt Cotton
Why doesn’t Bt toxin kills Bacillus
"
"

Bt gene is Specific ? Cry gene


Give any four major applications of Biotechnology?

> GM .

Crops
>
Waste Treatment

>
Diagnosis
> Processed food
M

>
Reduce reliance of
chemical pes€e@BAfB@Bs&
tolerance to abiotic stress
Increase
.

>

> In Gean Mineral Mage


value
Nutritional
Increase
.


Mm
B =
Bacillus

t a
thuringiensis
Bt Colton
cry
gene
Bt Bacteria

Inactive protein
active Inside the
→ becomes
gut of Insects
.
A

cry 1 As

Bacillus
IMDB.lt#itdllHAmn-
RNA interference .
Iftikhar .

s
MRNA > Proteins
DNA >
DNA → mRNA → proteins
n
dsRNA

¥9B *
£3
>
3

2
¥
§
.
¥
:<
Antenne
meloidegyne > c.
Dna > Sena

incognita
1+1
Steps in RNA i
> RNA interference
RNAi
HG %
A & B .

Chain A

4
BA chain B
Pro Insulin
A +B + c =

At B -
c =
Mature Insulin .
A 0 = A

B
is,
S
S
O - B
Eli Lily ± 1983 a 3 steps . HAMMAM
I

µ
Pro Insulin

Mature
Insulin .

A 1- B + c a
Pro Insulin

attire
A -1 B →g-M
Insulin .
r

Gene Therapy
Gene therapy is a collection of methods that allows correction of a
defective gene that has been diagnosed in a child or embryo.
Immune
ADA Enzyme =
function .

The first clinical gene therapy was given in 1990 to a four year old girl
child with adenosine deaminase (ADA) deficiency.
ADA deficiency can be cured by the following methods
Bone marrow transplantation

genetherapy
Enzyme replacement therapy .
Adenosine
deaminase

gene
therapy *F---B---Éu+
therapy .

Bone Marrow transplantation


r

SCID ✓ ADA deficiency

Permanent
gene therapy at
Embryonic Stage
-

cure .
ELISA

{
antigen -

Reaction -

T
-

Antibody
i

1. PCR
@-____g_

2. ELISA
Mm
no
Marga
µgdggggggAAAB-
B.
TRANSGENIC ANIMALS
Usesmnof Lurie Animals
i. To study
the Normal physiology G
Development
human diseases
study
.

2. To
products ✓
biological
.

3.
To Synthesize
To check vaccine safety
4.

5- To test the safety of drugs .


I -
I -

Antitrypsin =
Emphysema
>
Animals which express foreign
gene
.

To cheek the
Vaccine Safety
i.

2. To Study Human diseases .


> Rosie
Emphysema .

qBAyq
"

d- lactalbumin .

minimums
BioPatent

Patent granted
to
living organism .

Biopiracy
Biopiracy is the term used to refer to the use of bio-resources by
multinational companies and other organisations without proper
authorisation from the countries and people concerned without
compensatory payment.
µ
✓ ✓

Genetic Adenosine
deaminase
Engg
Approval
Comm He
MY gg
Bioolerourcer
of
.

Unauthorised are
>
car
.

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