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Synopsis
Major Applications of Biotechnology
Cry gene
=
Crystal protein
Bt Cotton Bt Brinjal
Bt Cotton
Why doesn’t Bt toxin kills Bacillus
"
"
> GM .
Crops
>
Waste Treatment
>
Diagnosis
> Processed food
M
>
Reduce reliance of
chemical pes€e@BAfB@Bs&
tolerance to abiotic stress
Increase
.
>
→
Mm
B =
Bacillus
t a
thuringiensis
Bt Colton
cry
gene
Bt Bacteria
Inactive protein
active Inside the
→ becomes
gut of Insects
.
A
cry 1 As
Bacillus
IMDB.lt#itdllHAmn-
RNA interference .
Iftikhar .
s
MRNA > Proteins
DNA >
DNA → mRNA → proteins
n
dsRNA
¥9B *
£3
>
3
2
¥
§
.
¥
:<
Antenne
meloidegyne > c.
Dna > Sena
incognita
1+1
Steps in RNA i
> RNA interference
RNAi
HG %
A & B .
Chain A
4
BA chain B
Pro Insulin
A +B + c =
At B -
c =
Mature Insulin .
A 0 = A
B
is,
S
S
O - B
Eli Lily ± 1983 a 3 steps . HAMMAM
I
µ
Pro Insulin
Mature
Insulin .
A 1- B + c a
Pro Insulin
attire
A -1 B →g-M
Insulin .
r
Gene Therapy
Gene therapy is a collection of methods that allows correction of a
defective gene that has been diagnosed in a child or embryo.
Immune
ADA Enzyme =
function .
The first clinical gene therapy was given in 1990 to a four year old girl
child with adenosine deaminase (ADA) deficiency.
ADA deficiency can be cured by the following methods
Bone marrow transplantation
genetherapy
Enzyme replacement therapy .
Adenosine
deaminase
gene
therapy *F---B---Éu+
therapy .
Permanent
gene therapy at
Embryonic Stage
-
cure .
ELISA
{
antigen -
Reaction -
T
-
Antibody
i
•
1. PCR
@-____g_
2. ELISA
Mm
no
Marga
µgdggggggAAAB-
B.
TRANSGENIC ANIMALS
Usesmnof Lurie Animals
i. To study
the Normal physiology G
Development
human diseases
study
.
2. To
products ✓
biological
.
3.
To Synthesize
To check vaccine safety
4.
Antitrypsin =
Emphysema
>
Animals which express foreign
gene
.
To cheek the
Vaccine Safety
i.
qBAyq
"
d- lactalbumin .
minimums
BioPatent
Patent granted
to
living organism .
⭕
Biopiracy
Biopiracy is the term used to refer to the use of bio-resources by
multinational companies and other organisations without proper
authorisation from the countries and people concerned without
compensatory payment.
µ
✓ ✓
Genetic Adenosine
deaminase
Engg
Approval
Comm He
MY gg
Bioolerourcer
of
.
Unauthorised are
>
car
.