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Course:

Basic Histology and Cytology


Mr Ibrahim B.O

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HISTOLOGY
● The study of cells, tissues and organs as seen with a microscop

● Derived from histos ( tissues) and Logia. ( Study/ science).

● It is the structural bases of cell ,tissue, organ and system


biology, physiology and pathology.
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Histology: 4 basic tissues

● Epithelial tissue

● Muscular tissue

● Nervous tissue

● CONNECTIVE tissue.
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EPITHELIUM
● simple(unilayered)
● squamous,cuboidal or columnar
● stratified (multilayered).
● stratified squamous, transitional epithelium,
stratified cuboidal/columnar,
pseudostratified columnar epithelium.
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Connective tissue
A. Areolar

B. fibrous/Dense

C. Adipose

D. Cartilage

E. Compact bone

F. Blood
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MUSCLE.
Straited/skeletal muscle : voluntary muscle supplied by
somatic nerves.e.g limb and girdle muscles

● Smooth muscle: typically in walls of hollow viscera,it is an


involuntary muscle supplied by autonomic nerves. Stomach,
oesophagus etc

● Cardiac muscle: like smooth muscle is involuntary and like


straited muscle show straitions. Posses inherent rhythmic
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Skeletal muscle
● Hundreds of nuclei
● Consist of long cylindrical fiber
● Nuclei are elongated and lie along periphery
● Membrane is called Sarcolemma
● Cytoplasm filled with longitudinal fibrils called myofibrils.
● Presence of transverse straitions of alternate A-bands
(dark region) and I-bands (light regions).
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Protein present in muscles
● Actin- possess head end ( extending into A band) and tail
end( anchored to the Z-line
● Myosin- possess tail end attached to M-line
● Actinin-. Found in region of Z disc. Bind tail.end of actin
filament to this disc.
● Myomesin- found at region of M disc, binds tail end of
myosin to the disc.
● Titin- connect head ends of myosin to Z disc and keeps
myosin filament in alignment.
● Desmin-. Link myofibril to each other
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Cardiac versus Skeletal
Similarities Differences
● Elongated fibres ● Cardiac fibre is not strict parallel,
● Myofibrils show transverse but branch.
● Cardiac Fibre is not
straitions
multinucleated but each myocyte
● A, I, Z, H bands present in has its nucleus
straitions ● Nuclear located centrally not
● Similar frame work and peripheral.
carpillary network. ● Sarcoplasm in cardiac abundant
● myofibrils; same structure of in mitochondria.
actin and myosin filaments ● Few myofibrils in cardiac
we continue from here
Thank you...

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