Leucopoiesis

Granular cells
Lesson 8

By Dr.Abdulrazzaq Alagbare at 1:07 am, Oct 24, 2013

White cells are a group of cells whose primary purpose is
to defend against bacteria, viruses, fungi, or other
foreign substances.
The most are granular cells
 contain enzymes used for digestion and destruction of
the invading organisms.
 Myeloid : Erythroid Ratio M:E
Normal value: 4:1
indicating that four myeloid, or white blood cells are
produced for one erythroid cell
There are three stem cells in the bone marrow

Plurpotent stem cell (PSC)

Myeloid stem cell (MSC)

Lymphoid stem cell (LSC),

1-myeloid stem cell (MSC)
or colony forming unit CFU-GEMM
1. G: granular,
2. E: erythrocyte,
3. M: monocyte
4. M: megacaryocyte)
2-Lymphoid stem cell (LSC)
which give the lymphocytes (T and B
lymphocytes)
Early progenitor cells of myeloid group colony-
forming units (CFU)

CFU-E CFU-Meg CFU-Eo

CFU-Bas CFU-GM
Leukopoiesis means the
state of

production, development,
differentiation of all white
blood cells
 Site of production
The main production of the non granular cells is the bone marrow
Regulation of production
Control mechanism of of granulopoiesis production mainly by the following growth factors

Site of production
The main production of the granular cells is the bone marrow
Regulation of production
Control mechanism of of granulopoiesis production mainly by the
following growth factors

• Colony-forming unit- For regulation and stimulation

granulocyte CSF -G of granulocytes
• Colony-forming unit- For regulation and stimulation
granulocyte monocyte of granulocytes and
progenitor cell CSF-GM monocytes
 Myeloblast

Promyelocyte

Myelocyte

Metamyelocyt

1%

5%

94%
Granulopoiesis maturation steps are

1-Myeloblast:
Nucleus :It has a large
nucleus with fine
chromatin,
Nucleoli:2–5 and scanty pale
blue
Cytoplasm : without granules
 Cluster designation
 (CD)45, CD38, CD34,
CD33,CD13,
 human leukocyte antigen
(HLA)-DR
2-Promyelocyte
 size: Slightly larger than a
myeloblast
 Nucleoli: less clear nucleoli.
 Cytoplasm: contains both
azurophilic granules and
characteristic neutrophilic
granules
Cluster designation
CD45, CD33, CD13, CD15
3-Myelocyte
 Size: Smaller than a
promyelocyte
 Nucleus: the nucleus is usually
oval
 Nucleoli: without
 Cytoplasm contains
azurophilic and neutrophilic
granules.)
Cluster designation
 CD45, CD33, CD13, CD15,
CD11b/11c
4-Metamyelocyte
 Nucleus: The nucleus is
indented (U-shaped)
 Cytoplasm : is pale pink and
contains neutrophilic
granules.
Cluster designation
 CD45, CD33, CD13, CD15,
CD11b/11c
5-Band Neutrophil (stab
form):
 Similar to the
metamyelocyte but with a
curved or coiled, often S
shape ,nucleus (without
clear lobes),
Cluster designation
 CD45, CD13, CD15,
CD11b/11c
6- Neutrophil :
 Nucleus : has 3
(occasionally 4 or 5) clear
lobes, separated by
chromatin threads.
 Cytoplasm: is pale staining
and contains neutrophilic
granules.
Cluster designation
 CD45, CD13, CD15,
CD11b/11c
The most important changes
which taken place during
their maturation, are;
 1 -granules appear in the
cytoplasm.
 2-after the promyelocyte is
the myelocyte in which
become clear cut, when the
differentiation into three
colors when stained by
Giemsa stain
The Monocytic Series
Origin of development:
 The bone marrow, It is part of the myeloid sera
Development steps are the following
 1-Monocytoblast --Monocyte
Control mechanism of of Monocyte production
mainly by the following growth factor:
 GM-CSF: For regulation and stimulation of
granulocytes and monocytes
 M-CSF : For regulation and stimulation
exclusively of monocytes
 1-Monocytoblast --Monocyte

 CD markers: CD33, CD13, CD14

 Normal neutrophils with 3-4 lobes
Band form with tow lobes
Polysegment neutrophil or (PMNs) over 5 lobes
Eosinophil 2 lobes
Basophils 2 lobes invisible

N. neutrophils Band form (PMNs) Eosinophil Basophils

That’s means all the mature white blood
cells which contain granular in their
cytoplasm and existing in the storage
places in three distinct cell
compartments:
• the bone marrow (production site)
• the circulation (Transport way)
• and the tissues. (activity site)
END OF THE LESSON
THANK U