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BLOOD CULTURE COLLECTION: WINGED SET (RECOMMENDED METHOD)

BLOOD CULTURE COLLECTION*


➔ Do not use damaged or expired bottles.
➔ Remove the “flip-cap”. Disinfect & allow to air dry.
➔ Collect 2-3 sets.
1 AEROBIC
➔ 10 mL of blood per bottle for an adult.
bottle
+ 1 ANAEROBIC ➔ Volume based on weight for pediatric patients.**
bottle
➔ Transport to the laboratory as quickly as possible.
= 1 SET
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RECOMMENDATIONS FOR BLOOD CULTURE COLLECTION

1 CHECK
2 3 4
trademarks belonging to bioMérieux , or one of its subsidiaries, or one of its companies. Any other name or trademark is the property of its respective owner / bioMérieux S.A. RCS Lyon 673 620 399 / Printed in France / thera / RCS Lyon B 398 160 242

PREPARE BOTTLES FOR INOCULATION PREPARE VENIPUNCTURE SITE COLLECT WITH WINGED SET
PATIENT ID
Palpate to find
& PREPARE Wash hands or the vein. Attach the
MATERIAL use an alcohol
Apply clean collection set to
hand rub. the adapter cap.
examination
gloves.
Remove the plastic To prevent
“flip-cap”. Disinfect
the skin. contamination, do
Disinfect the not re-palpate.
bottle septum and Allow the site to Insert the needle into
allow to air dry. air dry. the prepared vein.

5 BOTTLE INOCULATION
6 FINISH THE PROCEDURE
Record collection date, time
7 CORRECT LABELING DO NOT
Collect the aerobic
and site.
bottle first.
Ensure the bottle is Label bottles according to
correctly filled to manufacturer’s
the Fill-to Mark or recommendations.
target fill level, as Transport inoculated bottles as
shown. quickly as possible at room Leave cotton Replace the Position label in
Repeat for temperature to laboratory for over the septum plastic “flip-cap” the wrong place
anaerobic bottle. testing.

* Best practices for blood cul-


ture collection vary among CHECKLIST FOR WINGED COLLECTION SET
countries and healthcare Tourniquet
facilities: refer to guidelines Approved skin disinfectant
applicable in your institution. Blood culture set (ideally 2 to 3 sets; Gauze pads
This card is based on World 1 set = 1 aerobic bottle + 1 anaerobic bottle) Bandage
Health Organization recom-
Winged collection set Waste collection container
mendations for phlebotomy
(WHO guidelines on drawing Blood collection tubes (if blood Pen to record collection date,
blood: best practices in is needed for other laboratory tests) time, and collection site
phlebotomy-2010-ISBN 978 Blood collection tube adaptor (if required)
92 4 159922 1).
Gloves
** Pediatric bottles are NOTE: If other blood tests are required,
available. Disposable underpad always collect blood cultures first.

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