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● D efinition:Photosynthesis is the process by whichplants synthesize carbohydrates (e.g., glucose) from raw
materials using energy from light.
● 6CO2+6H2O-------------Light, Chlorophyll------------->C6H12O6+6O2
lants' Need for Food:
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● iving organisms require food for both raw materials and energy.
● Plants also need energy and raw materials, but they primarily use the air, water, and soil salts to create
carbohydrates like glucose.
● Photosynthesis is the process responsible for converting these raw materials into food using sunlight energy.
rocess of Photosynthesis:
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● hotosynthesis mainly occurs in the cells of leaves.
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● Water is absorbed from the soil by the roots and transported to the leaves through the xylem.
● Carbon dioxide is absorbed from the air through stomata in the leaves.
● In leaf cells, carbon dioxide and water combine to form sugar, using energy from absorbed sunlight.
● Chlorophyll, a green pigment in chloroplasts, absorbs light energy and facilitates the splitting of water
molecules into hydrogen and oxygen.
● Hydrogen molecules then combine with carbon dioxide to produce sugar.
● Photosynthesis is essentially the conversion of light energy into chemical energy in the form of
carbohydrates.
tilization of Photosynthetic Products:
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Glucose produced during photosynthesis is quickly converted to sucrose for transport.
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● Sugar not needed for respiration is turned into starch for storage or converted into other molecules.
● Starch is relatively insoluble and does not affect cell osmotic balance.
● Some plants store starch in stems or roots, while others have specialized storage organs.
● Sucrose, a soluble form of sugar, is transported through the phloem to all plant parts.
● It is used in various ways, including growth in buds, fruit ripening, and underground storage organs.
● Plant cell walls are made of cellulose, a long-chain glucose molecule.
espiration:
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● he sugar from photosynthesis can be used for energy through respiration.
● Respiration oxidizes glucose, producing carbon dioxide and water, releasing energy used for other chemical
reactions.
ectar:
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Nectar in flowers contains a mixture of sugars, including glucose, fructose, and sucrose.
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● Insect-pollinated plants produce and store nectar to attract insects for pollination.
Mineral Requirements:
Plants need nitrate ions (NO3–) to make amino acids, which are used to produce proteins and enzymes.
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● Nitrates are absorbed from the soil by the roots.
● Plants require magnesium ions (Mg2+) for chlorophyll production, which is essential for photosynthesis.
● Magnesium is obtained from salts in the soil.
Effects of Mineral Deficiency:
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● itrates and magnesium ions are essential mineral elements, and their deficiency can affect plant growth.
● Deficiency symptoms vary among plant species.
● Nitrate deficiency results in stunted growth, weak stems, yellowing and dying lower leaves, and pale green
upper leaves.
● Magnesium deficiency causes chlorosis, making leaves turn yellow from the bottom upward.
ources of Mineral Elements:
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Plants obtain mineral elements from the soil by absorbing ions from salts.
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● Salts, like potassium nitrate (KNO3), provide elements like potassium (K) and nitrogen (N).
● These salts originate from the breakdown of rocks into soil.
● Decaying plants and animals release minerals back into the soil, completing nutrient cycles.
Artificial Supplementation:
● In arable farming, nutrients are often depleted because crops are harvested and removed.
● T o replace missing minerals, farmers use methods like spreading animal manure, sewage sludge, or artificial
fertilizers.
● Common fertilizers include ammonium nitrate, superphosphate, and compound NPK (nitrogen, phosphorus,
potassium).
ater Cultures:
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● ater culture solutions can demonstrate the importance of mineral elements.
● A complete water culture contains salts providing all necessary elements for healthy plant growth.
● It includes potassium nitrate, magnesium sulfate, potassium phosphate, calcium nitrate, as well as carbon
dioxide, water, and sunlight.
● Hydroponics, or soil-less culture, is used in some horticulture practices, like growing plants in glasshouses.
ontrolled Experiments:
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● C ontrolled experiments involve setting up a control to ensure that results are due to the conditions being
studied and not other factors.
● Only one variable should be altered at a time to make valid comparisons.
● Hypotheses attempt to explain observations, and controlled experiments aim to test these hypotheses.
estarching a Plant:
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Testing for starch helps determine if photosynthesis is taking place.
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● To start, plants must be destarched by leaving them in darkness to convert starch to sugar.
● An iodine solution turns blue when mixed with starch, providing a test for starch presence.
Experiments to Investigate Photosynthesis:
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● everal experiments test the conditions required for photosynthesis.
● These experiments investigate the role of chlorophyll, light, carbon dioxide, and oxygen production in
photosynthesis.
● Results and interpretations provide insights into how plants use photosynthesis to produce energy.
aseous Exchange in Plants:
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● P lants exchange gases with the environment, taking in carbon dioxide and releasing oxygen during
photosynthesis.
● During respiration, plants consume oxygen and produce carbon dioxide.
imiting Factors in Photosynthesis:
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● T he rate of photosynthesis can be limited by external factors, such as light intensity, temperature, or carbon
dioxide concentration.
● Plants optimize photosynthesis when these factors are balanced.
● Glasshouses, polytunnels, and artificial supplements are used to maximize crop yields.
xperiments
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1. Effect of Changing Light Intensity on Photosynthesis:
● E xperiment: In this experiment, the rate of photosynthesisis measured by counting the number of oxygen
bubbles released by a pond plant (e.g., Canadian pondweed) under varying light intensities.
● Results: The rate of bubbling (indicating oxygen production)increases with higher light intensity. The
experiment demonstrates that photosynthesis is dependent on light availability.
● Interpretation: Photosynthesis relies on light energy.As the light intensity increases, more light energy is
absorbed, leading to faster photosynthesis.
2. Effect of Changing Carbon Dioxide Concentration on Photosynthesis:
● E xperiment: This experiment investigates how changingthe carbon dioxide concentration affects the rate of
photosynthesis in pondweed.
● Results: As the concentration of sodium hydrogencarbonate(a source of carbon dioxide) solution increases,
the distance traveled by the meniscus (a marker indicating oxygen production) also increases.
● Interpretation: Carbon dioxide is a crucial raw materialfor photosynthesis. Increasing its concentration
enhances the rate of photosynthesis since it's readily available for the process.
3. Effect of Changing Temperature on Photosynthesis:
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xperiment: This experiment, similar to experiment6, measures the rate of photosynthesis. However,
instead of varying light intensity, it focuses on changing the water temperature to understand the impact on
photosynthesis.
● Interpretation: Temperature plays a role in photosynthesis. Within a certain temperature range, higher
temperatures can increase the rate of enzyme-catalyzed reactions in photosynthesis. However, extreme
temperatures can denature enzymes, leading to a decline in photosynthesis rate.
Overall, these experiments illustrate that photosynthesis is a complex process influenced by external factors such as
light intensity, carbon dioxide concentration, and temperature. These factors can limit the rate of photosynthesis, and
optimizing these conditions can potentially increase plant growth and crop yield, which is vital for agriculture and
horticulture. Understanding these factors and how they interact with photosynthesis is crucial in plant science and
crop management.
Main Structures in a Leaf:
● E pidermis:A single layer of cells on the upper andlower surfaces, covered by a waxy cuticle to reduce
water loss.
● Stomata and Guard Cells:Structures in the epidermisthat control gas exchange (carbon dioxide in, oxygen
out).
● Mesophyll:The main photosynthetic tissue with tworegions: palisade mesophyll (more chloroplasts) and
spongy mesophyll.
● Vascular Bundles (Veins):Network of veins made ofxylem (carries water and salts) and phloem (carries
sugars and amino acids).
● Distribution of Chloroplasts:More chloroplasts inthe upper (palisade) cells, as they receive more sunlight.
● Stomatal Opening:Controlled by changes in guard cellshape and potassium concentration.
daptation of Leaves for Photosynthesis:
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● road, flat shape to increase the surface area for sunlight and carbon dioxide absorption.
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● Thin structure, allowing carbon dioxide to diffuse short distances to reach inner cells.
● Epidermis and cuticle reduce water loss and protect against pathogens.
● Mesophyll cells with chloroplasts perform photosynthesis, and their structure promotes efficient gas
exchange.