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Z
ebrafish, Danio rerio, is a tropical freshwater teleost tific experiments. To get a better insight into variability
belonging to the Cyprinidae family. The species has associated with polymorphisms, complete genomic se-
become a major research model used in biomedical quence information was obtained for homozygous wild-type
studies to investigate, for example, vertebrate development AB and Tübingen (TU1) individuals and a hybrid Sanger AB
genetics, physiology, and behavior (Grunwald and Eisen Tübingen (SAT1) line was generated by crossing them.
2002). The popularity of zebrafish as a model organism can In this article, we review some aspects of D. rerio breed-
ing in the laboratory environment. In particular, we describe
zebrafish sexual dimorphism and the effects of olfactory
Andrzej Nasiadka, PhD, is a research associate at the Zebrafish International
cues, visual stimuli, and social interactions on D. rerio re-
Resource Center, University of Oregon, Eugene. Matthew D. Clark, PhD, is
a staff scientist in the Vertebrate Development and Genetics Department, production. We also focus on factors influencing zebrafish
Wellcome Trust Sanger Institute, Cambridge, and is the Sequencing spawning. Then we describe breeding strategies commonly
Technology Development Team Leader, The Genome Analysis Centre, used in the laboratory environment and discuss the need and
Norwich, United Kingdom.
Address correspondence and reprint requests to Dr. Andrzej Nasiadka,
Zebrafish International Resource Center, 5274 University of Oregon, 1307 1Abbreviationsthat appear ≥3x throughout this article: SAT, Sanger AB
Franklin Boulevard, Eugene, OR 97403 or email andrzej@zebrafish.org. Tübingen; SNP, single nucleotide polymorphism; TU, Tübingen