Professional Documents
Culture Documents
Patient
Proper mindset/ open-minded
Curious
Make an observation.
Ask a question.
Form a hypothesis (scientific guess), or
testable explanation.
Test the hypothesis or conduct an
experiment.
Analyze the results.
Make a conclusion. LESSON 3: Laboratory Equipment
Laboratory Equipment
Goggles
o Most important piece of lab
equipment
o Protects eyes from broken
glass, chemicals, and flames
Beaker
o Measures liquid (but not
accurate)
o May be heated
o Used to hold or mix chemicals
Important Factors in Research/Variables in
an Experiment Erlenmeyer Flask
o Measures liquid (but not too
Independent Variable accurate)
o The variable that is changed in o May be heated
an experiment. o Used for mixing
o Cause of an experiment o Used in titrations
Poisonous
o Chemicals are dangerous if
ingested or inhaled
Radioactive
o Used as a warning to protect
people from being exposed to
radioactivity.
Flammable
o Chemicals are to be stored in
flame-resistant cupboard.
Irritant
o Substances labelled with the
irritant symbol are not
corrosive, but they can cause
discomfort and reddening,
irritation, or blistering of the
skin.
LESSON 4: The Microscope
History of Microscope
Types of Microscopes
Environment Hazard
o This symbol is attributed to Light Microscope
substances that pose a o The term light refers to the
significant danger to the process by which light
environment. transmits the image to the eye.
Monocular Microscope
Binocular Microscope
o Low Power Objective – 10x
Magnification
o High Power Objective- 40x
Magnification
o Oil Immersion Objective- 100x
Magnification
Biohazard
o This symbol is defined as
“those infectious agents Electron Microscope
presenting a risk or potential o A microscope that can magnify
risk to the well-being of man, diminutive details with a high
either directly through his resolution and gives 2D or 3D
infection or indirectly through view.
disruption of his
environment.”
Parts and Functions of a Microscope Stage Clips
o Clips on the stage that hold the
slide in place on the
mechanical stage
Aperture/ Iris Diaphragm
o The aperture is the hole in the
opening of the iris.
o The iris is the movable opening
that controls how much light
enters the camera.
Abbe Condenser
o This lens condenses the light
from the base illumination and
focuses it onto the stage.
Coarse and Fine adjustment controls
o Adjusts the focus of the
Eyepiece
microscope.
o The part that is looked through
Stage height adjustment
the top of the compound
o Adjusts the position of the
microscope
mechanical stage vertically &
horizontally.
Nosepiece
Mirror
o Holds the objective lenses and
o Reflects light into the base of
attaches them to the
the microscope
microscope head. It rotates to
Illumination
change which objective lens is
o Light used to illuminate the
active
slide or specimen from the base
Monocular/Binocular head
of the microscope.
o Structural support that holds &
Bottom lens/Field diaphragm
connects the eyepieces to the
o Knob used to adjust the
objective lenses.
amount of light that reaches
Arm
the specimen or slide from the
o Supports the microscope head
base illumination.
and attaches it to the base
Base
o Bottom base of the microscope LESSON 5: Levels of Organization
that houses the illumination &
Biological Organization
supports the compound
o Is the hierarchy of complex
microscope.
biological structures and
Objective Lenses
systems that define using a
o Lenses that directly observe the
reductionistic approach
object the microscope user is
Atom
examining.
o Smallest unit of ordinary
Specimen Slide
matter that forms a chemical
o Object used to hold the
element
specimen in place along with
o Smallest non-living unit to
slide covers for viewing.
build a living thing
Stage
o The platform upon which the
specimen or slide are placed.
Community
Molecules o Consists of population of
o A group of atoms bonded different species of organisms
together, representing the living together in a given area
smallest fundamental unit of a Ecosystem
chemical compound o Consists of all the community
Macromolecules of organisms and the sets of
o Macro means Large interactions that exist between
o A very large molecule bonded biotic and abiotic components
together Biome
o Composed of thousands of o Collection of ecosystems with
covalently bonded atoms similar climates and covering a
Organelles large geographical area
o Specialized structures that Biosphere
perform various jobs inside o Consists of the totality of the
cells ecosystems of the planet earth
o Means “Little Organs” o Pertains to any part of the
o Serves a specific function to earth’s sphere where life exists
keep an organism alive
Cells
o Building block of living things LESSON 6: Cell and Its Organelles
o Level where life begins
Tissue Cell
o Cells that are similar in o The simplest unit of living
structure and functions are matter
usually joined together to form Robert Hooke (1635-1703)
o English Scientist
tissues
Organs o Father of Microscopy
o When bunch of different o First to discover cell
tissues work together, they Cell Theory
o All living things are made of
form an organ
o Collection of tissues that cells.
o The cells are a basic structure
structurally form a functional
and function in all living
unit specialized to perform a
things.
particular function
Organ system o All cells come from pre-
o Group of organs that work existing cells
Matthias Schleiden and Theodor
together to perform a major
function Schwann states that “all living things
Organism are made up of cells”
o Living thing that has organized Rudolf Virchow states that “new cells
structure, can react to stimuli, come from preexisting cells”
reproduce, grow, adapt, and
maintain homeostasis
Population Parts and Functions of the Cell
o Group of organisms of the Cell Membrane
same species, occupying a o Separates the cell from
given area. the outside
o These organisms are freely environment
interacting with one another
Cytoplasm LESSON 7: TYPES OF CELLS
o Everything within the
Cell
cell membrane
o A structure containing a mass of
Golgi Apparatus cytoplasm surrounded by semi-
o “Packaging and permeable membrane called plasma
shipping center” of the membrane.
cell The cells are classified into two types:
Ribosomes Prokaryotic cell and Eukaryotic cell.
o Made of proteins and These terms were suggested by Hans
RNA Ris(1960s).
Nucleus
o Presentation are tools
that can be used as
lectures
Smooth Endoplasmic
Reticulum (Smooth ER)
o Involved in the
production of lipids
Rough Endoplasmic
Reticulum (Rough ER)
o Aids in the production
and storage of proteins
Mitochondria
o Powerhouse of the cell
Cytoskeleton
o Vast infrastructure of Prokaryotic Cells
microtubules and
microfilaments Unicellular cell structure
Centrosomes Cells without nucleus and other
o Responsible for the membrane-bound organelles, but a
production of nucleoid region is still present.
microtubules Most primitive cells and have simple
Lysosomes structural organization. It has a single
o Contain digestive membrane system.
enzymes for recycling Prokaryotes can be split into two
old molecules, proteins, domains: bacteria and archaea.
and structures Molecules of protein, DNA and
Vacuole metabolites are all found together,
o Membrane bound floating in the cytoplasm.
storage Nucleoid – a central region of the cell
Peroxisomes that contains its DNA
o Contain oxidative
Examples of Prokaryotic cells:
enzymes that aid in
digestion Bacteria
Centriole Viruses
o Responsible for cell Blue-green algae
division Mycoplasmas
Eukaryotic Cells It is important for cells to divide so you
can grow and so your cuts heal.
Multicellular cell structure
It is also important for cells to stop
Larger and more complex than
dividing at the right time. If a cell
prokaryotes, and usually contain
cannot stop dividing, this can lead to a
organelles that are absent from
disease called cancer.
prokaryotic cells.
This is because eukaryotes contain
membrane-bound organelles (like the
nucleus, endoplasmic reticulum, Golgi
apparatus, and mitochondria).
Anaphase
o The sister chromatids separate at
the centromere.
Telophase
Mitotic Phase (M phase) o A new nuclear envelop forms
around the chromosomes a
There are 2 major events that take place
in this phase which is the division of the nucleus reforms within the
nucleus and cytokinesis which is the nucleus the spindle fibers break
division of the cytoplasm. up and disappear.
o The process that separates the
duplicated genetic material
carried in the nucleus of a parent
cell into two identical daughter
cells.
Prophase Cytokinesis
o The chromatin (DNA and o The physical process of cell
Proteins) that makes up the division, which divides the
chromosomes condenses. cytoplasm of a parental cell into
two daughter cell.
Meiosis Metaphase I
o Homologue pairs—not
Is a type of cell division that takes place
individual chromosomes —line
in reproductive cells or sex cells.
up at the metaphase plate for
It produces gametes that contain half the
separation.
number of chromosomes.
o When the homologous pairs line
Diploid (2n) is the complete number of
up at the metaphase plate, the
chromosomes in a cell. 46 chromosomes
orientation of each pair is
for humans.
random
Haploid (n) is the half number of the
chromosomes in a cell. 23 chromosomes
for each gamete in humans.
Anaphase I
o Homologues are pulled apart
and move apart to opposite ends
of the cell. The sister chromatids
In many ways, meiosis is a lot like of each chromosome, however,
mitosis. The cell goes through similar remain attached to one another
stages and uses similar strategies to and don't come apart.
organize and separate chromosomes. In
meiosis, however, the cell has a more
complex task.
Prophase I
o The chromosomes pair with their
homologues in the process called
synapsis.
o The paired homologous form a
tetrad since it is composed for 4
chromatids.
Meiosis II
Prophase II
o Chromosomes condense and the
nuclear envelope breaks down, if Inheritance
needed. The centrosomes move
Gregor Mendel (1822-1884)
apart, the spindle forms between
them, and the spindle microtubules Father of Genetics
begin to capture chromosomes. He deduced that genes come in pairs
and are inherited as distinct units, one
from each parent.
An Austrian Monk who discovered the
Fundamental Laws of Inheritance
through a pea plant.
Metaphase II His Laws of Inheritance wasn’t
o Chromosomes line up individually understood until 1900’s. The genetic
along the metaphase plate. experiments Mendel did with pea plants
took him eight years (1856-1863)
Published his results in 1865. During
this time, Mendel grew over 28,000 pea
plants.
Anaphase II
o Sister chromatids separate and are Why did Mendel choose Pea Plant for his
pulled towards opposite poles of experiment?
the cell Pea plant easy to grow and can be sown
each year
Contains both male and female parts,
called stamen and stigma, and usually
self-pollinate.
Can also be cross-pollinated by hand,
Telophase II can be grown in a small area, and it
o Nuclear membranes form around produces lots of offspring
each set of chromosomes, and the
chromosomes decondense
Terminologies Homozygous genotype
Trait
Gene
Phenotype
Dominant
Recessive
Backcross
A gene that is less likely to be o the mating of a hybrid organism
expressed, represented by a lowercase (offspring of genetically unlike
letter (a) parents) with one of its parents or
with an organism genetically
similar to the parent.
Mendel’s Law of Assortment
o Mendel’s law of independent
assortment states that genes do
not influence each other with
regard to the sorting of alleles into
gametes: every possible
combination of alleles for every
gene is equally likely to occur.
o This law described that alleles of
various genes that are distributed
during gamete development
Mendel’s 3 Laws of Heredity assort independently of each
Law of Segregation (purity law of other.
gametes)
o This law explains that the pair of
alleles segregate from each other
during meiosis cell division (gamete
formation) so that only one allele
will be present in each gamete.
o During the formation of gametes
(eggs or sperm), the two alleles
responsible for a trait separate from
each other.
Law of Dominance
o Recessive traits are always Reproduction of Flowering Plants and How
dominated or masked by the Mendel Began
dominant trait. Self-pollination occurs when the
o The only way the recessive trait pollen from the anther is deposited on
shows-up in the phenotype is if the stigma of the same flower, or
the geneotype has 2 lowercase another flower on the same plant.
letters. Cross-pollination is the transfer of
pollen from the anther of one flower to
the stigma of another flower on a
different individual of the same species.
Mendel hand-pollinated flowers using
a paintbrush, he could snip the stamens
to prevent self-pollination covered
each flower with a cloth bag, He
traced traits through the several
generations
Mendel used pollen to fertilize Non-Mendellian Genetics
selected pea plants
Inheritance of traits that have a more
complex genetic basis than one gene
with two alleles and complete
dominance.
Does not follow the iconic Mendel's
Laws and can be defined as any
inheritance pattern that fails to follow
one or more laws of Mendelian
genetics.
Some traits exhibited blending where
the organisms’ offspring had two
separate traits from the parent,
meaning that certain alleles were not
dominant.
Characteristics
Class Demospongiae
o Ranges in size from small,
Leuconoid encrusting forms, through to large
o Sponges with a skeleton made irregular masses.
up of silicon-containing spicules o Colorful
or spongin fibers or both o Body type: Leuoconoid
o Can vary in size from small, o Ecology: Marine
encrusting forms to very large, o Example: Poecilosclerida
irregular masses
o Example: Spongia (Bath
sponges)
Class Homoscleromorpha
o Tiny spicules and encrusting forms
o Body type: Leuconoid
o Ecology: Marine
Classification of Porifera
o Examples: red encrusting sponge
Class Calcarea
o Spicules composed of calcium
carbonate
o Spicules are needle shaped or have
three or four rays
o Small in size and less colorful
o Body type: Asconiod, Leuconoid, or
Syconoid
o Ecology: Marine
o Example: Grantia