HISTOPATH PRE-EXAM HONING

15) Temp for water bath?

1) It refers to the rotting or decomposition 45 deg C
of the body due to bacterial action? 16) Common staining procedure in
PUTREFACTION Histopath?
2) The process by which the tissue is HEMATOXYLIN AND EOSIN
arranged in precise position in the mold 17) Natural dye from Mexican tree?
during embedding, on the microtome HEMATOXYLIN
before cutting, and on the slide before 18) Anionic dyes?
staining? ACID DYES
ORIENTATION 19) Purpose of descending concentrations
3) Paraffin wax substitute made up of of alcohol?
highly purified paraffin and synthetic REHYDRATION
plastic polymers? 20) Staining method that involves
PARAPLAST overstaining the tissue and then
4) Embedding mold consists of 2 differentiating the tissue details through
adjustable L-shaped strips of heavy decolorization?
brass or metal arranged on a flat metal REGRESSIVE STAINING
plate? 21) Staining method in which reaction
LEUCKHART’S EMBEDDING MOLD proceeds forward and is stopped once
5) The microtome used for EM is called? intensity of color is achieved?
ULTRATHIN MICROTOME PROGRESSIVE STAINING
6) A frozen section apparatus is called? 22) Staining method where tissue is
CRYOSTAT overstained and then differentiated or
7) Used for cutting celloidin sections? decolorized until only the desired
SLIDING MICROTOME elements remained stained?
8) Most dangerous type of microtome? REGRESSIVE STAINING
SLIDING 23) Staining method with the use of agents
9) Microtome invented by Paldwell Trefall such as mordants and accentuators?
designed to cut small and large blocks INDIRECT STAINING
of paraffin tissues? 24) Stain for neurofibril, axons, and
ROCKING MICROTOME dendrites?
10) Who introduced frozen sectioning? BIELSCHOWSKY’S
JULIUS CONHEIM 25) Stain for glycogen?
11) Designed by Adams for cutting PAS
celloidin-embedded tissues, considered 26) Stain for DNA?
the most dangerous microtome FEULGEN REACTION
because the exposed knife moves back 27) Special stain for glycogen?
and forth while the block holder remains PERIODIC ACID SCHIFF STAIN
stationary? 28) Stain for histones?
STANDARD SLIDING MICROTOME ALKALINE FAST GREEN
12) Type pf knife used only for celloidin 29) Stain for lipids?
work? SUDAN BLACK B
PLANO-CONCAVE 30) Mucicarmine stain is for?
13) Which of the ff is incorrectly matched? MUCIN
NONE OF THE ABOVE
31) Eosin stains?
14) The removal of gross nicks or ERYTHROCYTES, COLLAGEN, CYTOPLASM
irregularities on the knife edge and 32) Stain for copper?
grinding to restore sharpness? LINDQUIST’S MODIFIED RHODAMINE
33) Dye that is synthetic in nature? 49) Ideal time to perform fixation after
EOSIN interruption of blood supply?
34) Stain for melanin? 20 – 30 MINS
MASSON-FONTANA 50) Volume of fixative for museum
35) Method for calcium staining? specimens?
MODIFIED VON-KOSSA’S 50 – 100 times
36) Cationic dyes are those having a ___ 51) Order of tissue processing?
charge, and commonly known as ____? FIX-DEHYDRATE-CLEAR-IMPREGNATE
POSITIVE, BASIC 52) All are criteria for specimen rejection
37) Sudan III stains? except?
FATS ABSENT PATIENT’S MEDICATION
38) Carmine is produced from? 53) These are observable manifestations of
BUGS a disease?
39) Process of sealing the margins of the SIGNS
coverslip? 54) Failure of an organ to appear?
RINGING AGENESIA
40) Microscopic study of cells that have 55) Blockage or complete absence of an
been desquamated from epithelial opening or passage in an organ?
surfaces? ATRESIA
EXFOLIATIVE CYTOLOGY 56) Excision and examination of solid tissues
41) Color of superficial cells when stained from living subjects?
with Papanicolaou stain? BIOPSY
ORANGE TO PINK 57) Most reliable feature of malignancy?
42) Dye in Papanicolaou that stains nothing ABILITY TO METASTASIZE
and is often omitted? 58) For specimen with tumors, all of these
BISMARCK BROWN Y must be identified except?
43) Absence of these in sputum means NONE OF THE CHOICES
saliva was collected rather than 59) Most important component of all tumor
sputum? resections?
ALVEOLAR MACROPHAGES LYMPH NODES
44) Technique in autopsy where all organs 60) A tumor made up of nerve cells?
in thoracic, abdominal and pelvic are NEUROMA
removed at the same time? 61) Malignant tumor of epithelial cell origin?
EN MASSE CARCINOMA
45) Death of an organism as a whole, 62) Increase in size of tissues?
marked by cessation of functional HYPERTROPHY
activities of the different organs? 63) Clearing time of benzene?
SOMATIC DEATH 15 TO 60 MINS
46) Characterized by the cooling of the 64) Most rapid hardening and commonly
body, also known as “The Chill of used clearing agent?
Death”? XYLENE
ALGOR MORTIS 65) Becomes milky when dehydration is
incomplete?
47) Refers to the purplish discoloration of the XYLENE
skin due to pooling of blood in 66) Also known as dealcoholization?
capillaries? CLEARING
LIVOR MORTIS 67) Not a clearing agent?
48) Autopsy technique where organs were ALCOHOL
removed one by one? 68) Cytoplasmic fixatives: pH>4.6; Nuclear
VIRCHOW’S fixatives: pHN<4.6?
 BOTH ARE CORRECT DOLOR
69) Not an additive fixative? 88) The body’s response to an injury,
ACETIC ACID involving vasculature, chemotactic
70) Not a non-coagulant fixative? factors and immune cells?
ZINC SALT INFLAMMATION
71) Smallest aldehyde? 89) Stain for reticulin?
GLYCOXAL GOMORI’S
72) Fixatives that create a network that 90) Used for honing badly nicked knives?
allows solution to readily penetrate the FINE CARBORUNDUM
interior of the tissue? 91) Ideal fixative to tissue ratio?
COAGULANT 15-20:1
73) Most rapid fixative? 92) Normal hydrogen ion concentration of
CARNOY’S fixatives?
74) Not a non-coagulant fixative? pH 6-8
ALCOHOL 93) Primary goal of fixation?
75) Fixative for urgent biopsies? PRESERVE MORPHOLOGIC AND
CARNOY’S INTEGRITY OF THE CELL
76) Both a nuclear and histochemical 94) Fixatives that act on tissue without
fixative? chemically combining with it?
NEWCOMER’S NON-ADDITIVE FIXATIVES
77) Osmolality: Slightly hypotonic; pH: 95) Cytoplasmic fixatives contain glacial
neutral/6-8? acetic acid; Glacial acetic acid
I IS INCORRECT WHILE II IS CORRECT destroys mitolchondria and golgi
78) Highly explosive when dry? bodies?
PICRIC ACID I IS INCORRECT AND II IS CORRECT
79) Black precipitate of mercury can be 96) First and most crucial step in tissue
removed by? processing?
ALCOHOLIC IODINE SOLUTION FIXATION
80) Characteristic of an ideal dehydrating 97) All are functions of fixation except?
agent? NONE OF THE ABOVE
MUST BE ABLE TO DEHYDRATE WITHOUT 98) All of these factors are affecting fixation
PRODUCING CONSIDERABLE SHRINKAGE except?
OR DISTORION NONE OF THE CHOICES
81) Process of placing tissue in ascending
grades of alcohol?
DEHYDRATION
82) Deals with preparation of tissues for
microscopic examination?
HISTOPATH TECHNIQUES

83) Epithelia found in urinary tract lining?

TRANSITIONAL EPITHELIUM
84) Transitional epithelial cells are seen in?
URINARY BLADDER
85) Epithelial cells that form the lining of the
mouth, throat, and esophagus?
STRATIFIED SQUAMOUS
86) Father of modern pathology?
VIRCHOW
87) Cardinal sign which means pain?