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BIOLOGICAL
LABORATORIES
A S S I S T. P R O F. D R . C A N G Ü L T U N C AY A R N AV U T
Introduction to clinical laboratories:
Clinical labs are important in diseases diagnosis, determination its
severity and patient response to specific treatment.
Diagnosis of any disease is first done by physical examination by
clinician and confirmed by lab diagnostic tests.
Lab values are very important in determination of disease severity,
drug doses and in follow up.
Introduction
The sections of clinical laboratory are:
Clinical pathology
Hematology
Clinical biochemistry
Clinical microbiology
Serology
Blood bank
Histology and cytology
Introduction
Clinical biochemistry:
It deals with the applications of biochemistry laboratory to
find out the cause of a disease.
- Urine Samples
- Other Body fluids
Biochemical tests in clinical medicine
Lipid profile
Diabetic profile
Kidney profile
Liver profile
Bone profile
Electrolyte profile
Lab request and lab report forms
Lab request form: it fills computerize or paper filled by the doctor then send it to the
lab. The lab request contains a list of tests to be performed on specimen of patient. Each
lab has its specific request; for example, chemistry request, hematology request… etc.
Lab report form: it contains the result of patient.
Laboratory work flow cycle:
The flow cycle includes the entire steps of laboratory test, starting from test ordering by
a doctor until reporting the results.
Three phases of laboratory testing:
Pre-analytical: test ordering, specimen collection, transport and processing
Analytical-testing
Post-analytical: testing results transmission, interpretation, follow-up, retesting.
Disposable syringes Vacationer systems Disposable lancets
Phlebotomy
Phlebotomy or blood collection:
The term phlebotomy refers to blood
draw from a vein, artery, or the Gauze pads absorbent cotton Tourniquet
capillary bed for lab analysis or blood
transfusion.
Separator Gel
Serum
Separator Gel
Gold (and “tiger”) top tubes contain a gel that forms a physical barrier
between the serum and cells after centrifugation
No other additives are present
Gel barrier may affect some lab tests
Collection tubes
Brown and Royal Blue top tubes are specially cleaned for trace metal
studies
◦ Brown-top tubes are used for lead (Pb) analysis
◦ Royal blue-top tubes are used for other trace element studies (acid washed)
Blood collection tubes:
Plasma Separating Tubes (PST)
Top Color Additives Principle Uses
Lavender EDTA -The strongest anti-coagulant - Hematology
- Ca+2 chelating agent - Blood bank (ABO)
- To preserve blood cells components - HbA1C
(Glycosylated Hb)
Fibrinogen Fibrin
Top Color Additives Principle Uses
Black Sodium Citrate Ca+2 chelating ESR ( Erythrocyte Sedimentation
agent Rate)
to test how much inflammation in
the patient, unexplained fever,
Arthritis, Autoimmune Disorder
Gray -Sodium Fluoride Glycolysis Glucose tests
inhibitor
-Potassium Oxalate Anti-Coagulant
Royal Blue Heparin Anti-Coagulant Toxicology
Na-EDTA Tube should not Trace Elements and metals
be
contaminated
with metals
Yellow ACD ( Acid-Citrate Anti-Coagulant DNA Studies
Dextrose) Paternity Test
HLA Tissue Typing
(Human Leukocyte Antigen)
The body used this protein to
differentiate the self-cells from non-
self cells
Serum Separating Tubes (SST)