TECHNOLOGY

BANNARI AMMAN INSTITUTE OF University, Chennai)

Institution Affiliated to Anna
(An Autonomous
SATHYAMANGALAM -638 401

Stay Ahead PERIODICAL TEST |- AUGUST 2023

VII Semester
INSTRUMENTS
18EI702 & ANALYTICAL
Degree & Branch: B.E & El
Maximum: 50 Marks
Time: 1:30Hrs.
nstructions:
1
oSnould not mark/write anvthing on the Question Paperother than the
register number.
Section C contains questions for 15 MarkS.
2 contains guestions for 30 Marks each. limited to 20 in secion A & B.
onAandB of the Ouestion Paper subsections in each section. Maximum mark is
3. Scan attempt answering anv
two out of three
and 10 in sections C.
Questions
.No.
COURSE OUTCOME 1 MAXIMUM: 20 MARKS
SECTION A is less compared to
analvzed that the light transmitted by the other end of the material by the molecules
A1 Beer lambert
A1.1. This is because of. the absorption of the light source
Incident light in fiqure solution used.
the sample. This indicates the concentration of the sample
present in

Incident Light Transmitted Light

Experimentation
Figure A1.1. Beer
the concentration of the sample solution by referring the
() Formulate the proper relation to predict
incident light l, and transmitted light I and path length L.
the (2 Marks- [U/C,2])
figure A1.1which clearly shows
the proper sequential order to demonstrate the
components in scenario using single beam
(i) Arrange the below given discussed in above
experiment done by Beer Lamberts sample chemical solution.
spectrometer to find the concentration of
1 Photo Detectors
2 Filter
3. Sample
4. Light Source
5. Read out Device (4 Marks- [An/C,

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 Find out the missing parts in the below figure A1.2 if the beer lamberts USed the double be

of sample solution in his experimentati

technique for determining the concentration
(ii)

Ampliier
A

B
Detector
IR Source

C
Figure A1.2. Double Beam Spectrometer
(4 Marks- [Ap/C,2)
describes the
A2 Figure A2.1 shows the construction of Fourier transform Infrared spectroscopy and
analysis of samples of typical infrared absorption frequencies in the isform of a spectrum. The beam
Splitter is fixed in such a way to make proper pattern of fringes. Mirror half silvered to develop proper
reflected rays.
Mirror 1

Mirror 2

Light
Source

Beam Splitter
Half Silvered Mirror

Detector

Figure A2.1. Michelson Interferometer

Predict the mirror which is movable and tixed among the two mirrors 1 and 2 used in above
fiqure A2.1 to produce proper fringes of patern tor detecting
accuracy. Justify concentration of sample with more
(iü) Indicate the changes happens in Michelson (3 Marks- [Ap/C,2)
interrerometer
A2.1 is full silvered mirror instead of half silvered mirror
if the beam splitter used in figure

Infer the changes that happen in he (3 Marks-[U/C,2)

onresentation, if the angle of the beamInterrerometer experimentation in figure
splterIS Changed as 90 degrees A2.1with pictorial
inclination. instead of 45 degree
A3 The flame emitted from the volcano is analyzed to predict the various (4 Marks- [UIC,2))
chemical
L.enocial technigues called Flame Emission spectrometer displayed in figure A3 mixtures
4 present in it

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 Air from
Compressor

Lens Detector|
Monochromator Recordet
Fuel

Neublizer

Flame
Sample

Figure A3.1. Flame Photometer

Yes/NO with the below mentioned four
(1) Relate the proper parts in figure A3.1 by marking on nature of operation,
important system of flame emission spectrometer based
Nebulizer Flame Mono
Lens Detector Recorder
Pats Sample Com
chromator
8Syslem pressor

Yes ? ?
Emission
System

Optical No ? ? ?
System

Detection ? No ? ?
Syslem

Recording ? ? No
System

(5 Marks- [U/G2])
Reframe the above block diagram in fiqure A3.1 to make it suitable for analyzing the atoms
(ü) absorbed in sample chemical solutions.
(5 Marks-[An/P 2])
SECTION B COURSE OUTCOME2 MAXIMUM;20 MARKS
Consider the pharmaceutical industry in which the separation of individual components from a complex
31
is a challenging role in the effective production of drugs at the required concentration. In order
mixture is The
to do this better, special techniques called gas chromalography as shown In fiqure B1,1 are used.
recorder displays the graphical representation of output called Chromatograph, Each peak represents
the concentration of compounds in gas.

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 Flow Controller Injector Port

Record
Column Detector

Column Oven

Carrier Gas
Fiqure B1.1. Gas Chromatography
Given the statements about the process of capillaryelectrophoresis in the above scenano is not
In proper order, Arrange the correct sequence which make these process in better manner
A All ions, positive, negative, are pulled through the capillary in the same direction electro
osmotic.
8. The migration of the analyses is initiated by an electric filed that is applied between the
source and destination vials and is supplied to the electrodes by the high voltage power
C
supply.
The output of the detector is sent to a data output and handling devices such as an
integrator or computer. The data is then displayed as an electropherogram, which
reports detector response as a function of time.
D Sample is introduced into the capillary action, pressure, siphoning or electro kinetically
and the capillary is then returned to the source ial.
E. The analytes separate as they migrate due to their electrophoretic mobility, and are
detected near the outlet of the capillary.
(5 Marks- [An/P.2])
() Predict the sequence of chemicals species exit gas chromatographic column by referring the
below graphical representation (chromatograph) based on the peaks 1, 2,3,4,5 shown in figure
B1.2.
(3 Marks- [(An/C.2|)

Time
Fiqure B 1.2. Chromatograph
(ii) Predict the column which has greater efficiency among thecolumn A and B used in gas
chromatography for separating complex gas liquid mixture mentioned in below graphical
representation.
(2 Marks- [Ap/C,2])

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 CohuEL A

Rrpoa CorposrdY
Uttedpks
Congousd X

Tune (suxs)
CenpoY
Cokina B
CopoualX
Repome

Tuue (a)

Figure B1.3. Efficiency Graph

soluble in
B2 Liquid chromatography is a conventional technique used for the separation of compounds
chromatography is shown below in fiqure B2.1.
the liquid phase. The schematic of the liquid

Oulet Solution
Solveat Reservolr

Figure B2.1. Liquid Chromatography

Represent the missing parts A, B, C, D and E in figure B2.1 which is necessary to analyze the
complex liquid mixture by the liquid chromatographic technique discussed in above scenario.
(5 Marks- [UIC,2)
dentify the high and low characteristics response of liquid chromatography based
(ii
on the elution of solution peuennttalion by refering figure
Bz.2.
(2 Marks- [U/C,2)

ton voe r)
Figure B2.2. Performance Characteristics

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 (ii)Match the following with the suitable pumping system for injecting sample in
column based on its function
(3 Marks- (Ap!C,2h chromalographic
Type Schematic
Eiow is
Function
Syringe (or) Displacement dependent on solvent
viscosity and Back Pressure
Pumps Outet (C1)
Pision
(A1)
Vahe

hlet
Chedk
Head Vake
Scal

(B1) Produce Pulsed Flow

Pneumatic (or) Constant (C2)
Pressure Pumps
(A2)

(B2) Flow is independent of

Reciprocaing Purmps Controler
Pressure
Tank Viscosity and Back Pressure
(A3)
(C3)

+To Hoe

(B3)
B3 In certain chemical industries like petrochemical industries, it is necessary to separate the various
components from the crude oil mixture using High Pressure liquid chromatographic technigue as
shown in figure B3.1

Sample
Inject n port
reservolrs vacuum
solvert1solvent 2 pump Flcw
piter
Prossuro
Degasser1| Dogassor 2 pre
column
gauge

fmixlng vessel
Analytlcal
column

Hoh pressure pump

dfferential detector
to waste to waste or
Figure B.3.1HPLC System fractlon collector

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 (6) If reciprocating pump is introduced in the place of High Pressure Pump. Interpret the operational
change of HPLC system in figure B3. (5 Marks- (UIC, 2)
(ü) nprele the conditional relation by marking increases or decreases between various
paramelers of HPLC system shown In figure B3.1.
number of plales per
Column length Increases
unit area
column length Increases
Resolving power ?
Surface area
(3 Marks- [Ap/C.21)
Particle size Increases
of the HPLC system by analyzing the below
(i) Indicate the gradient and isocratic conditions (2 Marks- (U/C,2))
response.
70
A

52 30
B

10 15 20 25

Time, minules

Fiqure B3.2. Response Characteristics MAXIMUM: 10 MARKS

COURSE OUTCOME3
SECTIONC oxygen
It is mandatory to increase the free effluent
the human respiratory process.amount
Oxygen plays a vital role in balanced ecosystem. Thus the of oxygen present in air and
air in the atmosphere for a physical methods as shown in figure C1.1.
industries is analyzed using chemical methods and
Voltage Supply
Galvanometer
Platinum
Cathode
-AgiAgCl Anode

KCL Electrode
Solution
Teflon Membrane

Figure C1.1. PH Electrode

The cell reaction of the electrode is given as,
Pb +Pb +2e
O, .2H,0 +4e 40H
Predict the cell reaction that takes place in Anode and Cathode among the above equations
during the electrochemical process happened in above figure C1.1.
(2 Marks- (Ap/C,2)
Construct the suitable structure for analyzing the amount of Oxygen content present in the air
(Ö)
without using chemical solutions with the help of below fiqure C1.2.
(3 Marks-[Ap/C 2)

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