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Introduction to

interamnetite
chromatography
Chromatog (LC) : MP ovesinos

I
-

is :
avanviamo sample minivas feramosiaainitasmes extri lo mi la 10 mi
: a column oo detector name = Pistones avas anonas
=
amadivan selectivity factor the ori uns no inkman t
: in
degradation products Inicures GC inst LC Intrinsica
Interion i
·
minim

joine : Monastic Chromophore malo detector witoolws


: mossrailorime sample invention incon impured s
must
organic solvent verman worrients ins list i inoniss
·
or

-
differed : Quantitative analysis of phar products =
powionom
&me :
Monitoring of the stability and quantitation of degradation =
numains is 100 modegrade
If of pure
drug & formulation notio control insirprintoxic vois pure A formular
: Measurement of drug and metabolites newamis lo metabolites =

Determination of partition coefficients pla values protein


binding
:
, ,

:
Qualitative Peinsample nizen (HPLC finger print)
analysis =

Mode :
Adsorption : o Liquid-Solid Chrom. Bir of 1800 absorb silica distri
1 #

=Sp
=normalize d adsorption description for solute

in
adsorption
separa
solid Sp
analyst of absorb or
-
-
one un

tion -

page description un MP

solid Cilica Alumina a s


(Hexare/Tetrahydrofuran
MostIsinate silica/Alumina
-
MP w
organic solvent (
=Silica Siland =
SiCH (polar interact
H-band) elute stra

O
win

i H o-H
sp :
capacity Eri lad sample m
=
i

pH range =2-8>8 pH silica begin :

o-
j j -
- -

j <2 :
pl in siland
group me
=
Alumina
n i

rojp capacity
· -A
:
risne
a

numitztwsine
+

Als

pHrange = 4 5 .
-

9 5 .

C
on polarity as Elvent Strengthe
! Partition =

Liquid-liquid chrom
.

mpolarity i Elvent
Strength is I I
notemillen to partition the solute -
dissolved to ligd Sp=/bissagin
arms Po sp
dissolveda state
one partition to
elute


-
MP
ing ins Esrilular I
O
·

C
Silica base ; pH2-8

I
conser Kid s SP Banded phase chrom
& MP
-
:
.

-> Polymer base ; ple-18

↳ NP ↳ NP
Gues Sp1/band in solid Organic solvent

·
in e trotte =
particle =

1G
,



did
cyano , amino
=
CHz ,

int
->
ji - G RP I
Co Go Phenyl RP
Wonpolar
H20+
Organic solvent

o
g
-

, ·
,
o -si- O
I
-

si
I
-

C +
3HC) CHy
↳ Mp= polar elment se
G u
·
indo not Silica tagnump
mixed
O CH Tein ms adsorpsion

18 peak or
sym

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