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Analyst 2
Analyst 2
interamnetite
chromatography
Chromatog (LC) : MP ovesinos
I
-
is :
avanviamo sample minivas feramosiaainitasmes extri lo mi la 10 mi
: a column oo detector name = Pistones avas anonas
=
amadivan selectivity factor the ori uns no inkman t
: in
degradation products Inicures GC inst LC Intrinsica
Interion i
·
minim
-
differed : Quantitative analysis of phar products =
powionom
&me :
Monitoring of the stability and quantitation of degradation =
numains is 100 modegrade
If of pure
drug & formulation notio control insirprintoxic vois pure A formular
: Measurement of drug and metabolites newamis lo metabolites =
:
Qualitative Peinsample nizen (HPLC finger print)
analysis =
Mode :
Adsorption : o Liquid-Solid Chrom. Bir of 1800 absorb silica distri
1 #
=Sp
=normalize d adsorption description for solute
in
adsorption
separa
solid Sp
analyst of absorb or
-
-
one un
tion -
page description un MP
O
win
i H o-H
sp :
capacity Eri lad sample m
=
i
o-
j j -
- -
j <2 :
pl in siland
group me
=
Alumina
n i
rojp capacity
· -A
:
risne
a
numitztwsine
+
Als
pHrange = 4 5 .
-
9 5 .
C
on polarity as Elvent Strengthe
! Partition =
Liquid-liquid chrom
.
mpolarity i Elvent
Strength is I I
notemillen to partition the solute -
dissolved to ligd Sp=/bissagin
arms Po sp
dissolveda state
one partition to
elute
↳
-
MP
ing ins Esrilular I
O
·
C
Silica base ; pH2-8
I
conser Kid s SP Banded phase chrom
& MP
-
:
.
↳ NP ↳ NP
Gues Sp1/band in solid Organic solvent
·
in e trotte =
particle =
1G
,
↳
⑮
did
cyano , amino
=
CHz ,
int
->
ji - G RP I
Co Go Phenyl RP
Wonpolar
H20+
Organic solvent
o
g
-
, ·
,
o -si- O
I
-
si
I
-
C +
3HC) CHy
↳ Mp= polar elment se
G u
·
indo not Silica tagnump
mixed
O CH Tein ms adsorpsion
↳
18 peak or
sym