Sem test 1 .

Northern Blot @JohaneCilliers

CYR 16 gene
+ BMP
-
BMP

stronger signals
. : BMP has effect

÷
:÷÷÷¥
mRNA processing

introns

transcend
more

0 g-
up
mature mRNA
:
←

-
difference
-

degradation cytoplasm :
.

none : bands
closed :
ChIP it all : mono then
✗ all AC
bene open y
.

mononuclearsome
t

o
✓
r Me detected :
CLOSET 31-11 recruits
t
all histories
Dnmt 'S AC
closed open .
 TUES :

Northern Blot Nuclear run on assay

1L-10 dttect :
expression .

{ relatively

at
Iwo :X
same
control expression
transcription initiation .

-
differential expression :
i.

notranscript genes post trans level . .

detected .

only one abundant- -

enough → detect :
an ✓ transcribed : nucleus
absence
←
housekeeping gene conditions : contradicts
-
tells : experimental
correct
-

Northern Blot : ✗ sensitive enough

transcript amounts
.

to detect low
u

small amount
>
present

@JohaneCilliers

halt life in presence :L-10

longer a

regulate
expression :

post
-
Eransc .

level .
TUT 7
become
.

signals
less

i closed chromatin

②
✗ digested ←
(less cleavage)

more digestion -
( more cleavage)
open chromatin

-
southern Blot
@JohaneCilliers

control
C housekeeping ) similar Ac
gene }
highly AC
-

} ✗
due
expressed
: low Ac