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Effects of Ginger (Zingiber Officinale), Lemon (Citrus Limon) and Garlic (Allium Sativum) Extracts Against Houseflies (Musca Domestica)
Effects of Ginger (Zingiber Officinale), Lemon (Citrus Limon) and Garlic (Allium Sativum) Extracts Against Houseflies (Musca Domestica)
In Partial Fulfillment
of the Requirements for the Subject
Work Immersion
Jerlene G. Viray
Mark Joshua Verosil
Jolan M. Victorio
Rafoncel Raquin
Mikaella Reighn C. Jota
Arjay F. Garingarao
TABLE OF CONTENTS
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TITLE PAGE........................................................................................................ i
APPROVAL SHEET............................................................................................ ii
ACKNOWLEDGMENT....................................................................................... iii
TABLE OF CONTENTS...................................................................................... iv
LIST OF TABLES................................................................................................ vi
LIST OF FIGURES.............................................................................................. vii
ABSTRACT.......................................................................................................... viii
CHAPTER
I. INTRODUCTION
Background of the Study............................................................................ 1
Statement of the Problem........................................................................... 3
Significance of the Study........................................................................... 4
Definition of Terms.................................................................................... 4
Conclusions............................................................................................. 22
Recommendations................................................................................... 22
ABSTRACT
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CHAPTER I
INTRODUCTION
chemopreventive agent. Here, we show that whole ginger extract (GE) exerts significant
sickness prevent us from drawing any firm conclusion. The current review for the first
time summarizes the results. An attempt is also made to address the lacunae in these
published studies and emphasize aspects that need further investigations for it to be of use
Ginger is a medicinal plant that has been widely used in Chinese, Ayurvedic
and Tibb-Unani herbal medicines all over the world and has a long history of use in
traditional systems of medicine. The primary pungent agents are due to the presence of
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phenylalkylketones or vanillyl ketones. Gingerol and shogaol are two most active
ginger rhizome can be applied for the prevention of nausea and vomiting in motion
al.,2021).
nausea and vomiting. Ginger extracts accelerate gastric emptying and stimulate gastric
antral contractions. These effects are mainly due to the presence of gingerols and
(Giacosa et al.(2015)
anti-oxidant substance and may either mitigate or prevent generation of free radicals. It is
considered a safe herbal medicine with only few and insignificant adverse/side effects
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Numerous experimental and clinical trials have proven ginger for its range of
hepatoprotective etc properties. The present article aims to explore traditional Unani and
pharmacological activities of this herb reported till date (Ansari et al. 2021)
This study aimed to determine the Effects of Ginger (Zingiber Officinale) Extracts
against Housefly (Musca Domestica) . Specifically ,it sought answers to the following
questions:
of:
a. The amount of time the housefly will killed and the number of housefly died;
3. Is there a significance different between the effect of ginger extract and commercial
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Significance of the Study The result of this study will be of great benefit to
the following:
Communities: Those barangays who are affected of swarm of flies specially those who
are near on poultries and piggery can make their own housefly repellent during the
Future Researcher. The study may be used as a reference in conducting new research or
testing the validity of the related findings. This study will also give them a background
Domestica)
Carinderia /s: It will help them to give knowledge to avoid swarm of flies during
Definition of Terms
Effects. This refers to the change that results when something is done or happens.
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Pesticide: This refers to any substance or mixture of substances intended for preventing,
weeds, or any other forms of life declared to be pests; and any other substance or mixture
CHAPTER II
This chapter presents the related literature and studies to which the present study
is smilar or related. The following review aims to support and provide the researchers
with the necessary background and information for deriving and interpreting the findings
in this study.
About Ginger
Zingiberaceae, used As a spice, food, flavouring agent, and medicine. Its generic name
Zingiber is derived from the Greek zingiberis, which comes from the Sanskrit name of
the spice, singabera. The Latin Name, Zingiber, means “shaped like a horn” and refers to
the roots, which resemble a deer’s Antlers. Long cultivated by the ancient Chinese and
Hindus, Ginger was one of the first Oriental spices known in Europe. Throughout the
early centuries, Ginger was thought to have Medicinal powers. It was often used by
pregnant women for morning sickness. The spice has a Slightly biting taste and is used,
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usually dried and ground, to flavour breads, sauces, curry Dishes, confections, pickles,
and Ginger ale. Its fresh rhizome is used in cooking. It is most Alkaline-promoting foods.
It has a pH of 5.6 to 5.9, similar to that of figs, fennel, leeks, Parsnips and romaine
lettuce. The alkalinity of foods depends on many variables, including Growing conditions
Food is one of the essential fundamental elements needed For human survival1.
Food comprises edible plant parts (such as fruit, nuts, corms, leaves) and animal tissues
which May be cooked or eaten raw depending on the cultural Orientation of the
population. Whether eaten raw or Prepared into delicious delicacies, food provides:
energy And nutrients for proper nourishment of the body, maintains The body, repairs
worn-out tissues and promotes growth of The body1,2. Food is not only consumed for its
nutritional Benefits, but individuals’ preference for a given food is Mainly inspired by
their cultural values, sensory qualities Such as taste, texture, palatability, mouth feel and
flavourings and flavour enhancers to help Enhance the flavour of natural foods3 Rajathi
et al.(2017).
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Ginger is used worldwide as a cooking spice, condiment and herbal remedy. The
Chinese have used ginger for at least 2500 years as a digestive aid and antinausea remedy
and to Treat bleeding disorders and rheumatism; it was also used to treat baldness,
(TCM), ginger is considered a Pungent, dry, warming, yang herb to be used for ailments
medicine of India, to block excessive Clotting (i.e. heart disease), reduce cholesterol and
fight arthritis. In Malaysia and Indonesia,Ginger soup is given to new mothers for 30
days after their delivery to help warm them and to Help them sweat out impurities. In
Zingiberaceae originating from southeast Asia.1 The main components of ginger include
starch, lipids, proteins, and inorganic compounds.2 It is widely used around the world as
a flavoring or fragrance In foods, but also in traditional oriental medicine since ancestral
times.3 For centuries, ginger has been an integral part of the various traditional And folk
systems of medicine in China and India for the treatment of Different diseases, such as
headaches, colds, osteoarthritis, muscle Pains, nervous diseases, gingivitis, toothache, and
asthma.3–8 Currently,There are many studies that devoted to specific aspects of ginger’s
actions. For example, Stoilova et al studied the antioxidant effect of ginger Extract.9
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isolated from ginger.11 Besides, Ginger has also shown anticarcinogenic, immune
Li et al.(2019).
Gingers are perennial herbs that belong to the large family Of Zingiberaceae. Ginger
ranges in height from B10 cm to 8 m. Some species are almost prostrate near the
ground.Gingers have an aromatic rhizome that is subterranean or Above ground, and each
can produce a leafy shoot. The Inflorescences are terminal either on the leafy shoot or on
The leafless shoot. Ginger flowers are fragile and have a Short vase life. In most genera,
they emerge from base to Apex of the inflorescence [1] Ismail et al. (2016).
Phytochemicals of Ginger
phytochemical analysis and toxicity of methanol extract of Etlingera elatior (torch ginger)
flowers.The extract was effective on tested microorganisms And MIC values were in the
range of 1.563-50.000 mg/mL. The brine shrimp lethality test Exhibited no significant
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toxicity (LC50 = 2.52 mg/mL) against Artemia salina. The E. elatior Flower extract with
high LC50 value signified that this plant is not toxic to humans. While the Phytochemical
screening of the flower extract revealed the presence of the following compounds:
anthraquinone And reducing sugars were absent. The concentration of the flower extract
required for 50% Inhibition of DPPH radical scavenging effect (IC50) were 9.14 mg/mL
extracts. The highest activity was observed with the methanolic extract of Z. officinale
from zone 3 at the concentration of 50 mg/ml against P. aeruginosa (31.6 mm). Likewise,
the methanolic extract of Z. officinale obtained from zone 3 had the greater MIC toward
P. aeruginosa (12.5 mg/ml). Whereas, the values of MBC of methanolic crude extract of
Z. officinale obtained from the three zones are the same (50 mg/ml). The study shows the
cyanogenic glycosides. Ginger (Zingiber officianale) had the highest phenolics content of
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mg/g was observed in the study with the mushroom, Boletus spp. Ginger also had the
of mushrooms, ginger and garlic and high phenolics and Flavonoid contents of ginger and
Jiwuba et al.(2014) revealed the presence of crude fibre (10.36%), ash (6.57%),
ether extract (6.48%), nitrogen free extract (64.82%) and crude protein (5.45%). Results
also showed that GRP was low in both essential and non essential amino acid groups with
the total amino acid value of 25.61 g/100g. The predominant mineral elements were zinc
(Zn), manganese (Mn), copper (Cu), calcium (Ca), iron (Fe), sodium (Na), phosphorus
(P) and potassium (K). The mineral values followthe order of Na (38.96 µg / g) > K
g) > Fe (1.59 µg / g) > Cu (0.86 µg / g). The results revealed that ginger rhizome powder
contain moderate level of pharmacological active compounds as well asfeed nutrients and
these plants significantly (P<0.05) decreased the body weight and serum lipids including
total lipid, triglyceride and low density lipoprotein when compared with positive control
group, while they significantly (P<0.05) increased high density lipoprotein when
compared with positive control group. Furthermore, our study showed that the
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combination of these three plants exhibited better effects than when compared to the
individual powder of the plants. In this study we compared the effects of separated and
combined administration of cinnamon bark, ginger root, green coffee beans as anti-
hyperlipidemic rats, while the combined administration showed more powerful effects.
Previous reports on cinnamon, ginger and green coffee as separately administered were
thus confirmed; however, this is, to our knowledge, the first report dealing with the
combined form of the three plants. Such combined administration might be useful in
Housefly
Iqbal et al. (2014) explained role of House fly as a disease vector of humans and
livestock. This article highlights various aspects of the life of the said pest that includes
its general description-morphology, biology, life cycle, its pest status and major control
as it passes through all stages of development like egg, larva, pupa and adult which takes
10 to 14 days for its completion. Its life span is between 15 to 30 days. With respect to
control strategies of the said pest, it was reviewed that chemical control is the most
common and efficient technique but dependence on insecticide for fly control is
Biological control with study explains role of House fly as a disease vector of humans
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and livestock. This article highlights various aspects of the life of the said pest that
includes its general description-morphology, biology, life cycle, its pest status and major
control strategies.
Banjo et al. (2005) aimed The housefly (Musca domestica L.) is known to be a
vector of diseases. These flies are prevalent in items that are exposed.
functional profile mature alongside their host and putative host-microbe relationships are
Jonge et al. (2020) identified microorganisms furtherrecovered from the house fly
are serious pathogens, known to produce diseases such as meningitis, food poisoning,
exception is Bacillus thuringiensis, a known pathogen for insects that only occasionally
produces allergic reactions in humans. If these organisms are not prevented from entering
the food preparation and consumption areas, they could become a serious risk in the
transmission of disease fly are serious pathogens, known to produce diseases such as
colitis. The possible exception is Bacillus thuringiensis, a known pathogen for insects that
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only occasionally produces allergic reactions in humans. If these organisms are not
prevented from entering the food preparation and consumption areas, they could become
Murvosh et al. (1964) studied the house fly for many years, surprising gaps exist
in our knowledge of the basic biology of this species. House fly mating behavior
described in rather general term, but only few detailed investigation have been reported in
the literature. The research presented here in was conducted to add this fund of necessary
and medicinal potential of ginger. This review deliberates chemical composition as well
as the most recent research findings on potential health benefits of ginger, including its
Shahrajabiana et al.(2019) Ginger (Zingiber officinale) has been used as a spice and
medicine for over 200 years in Traditional Chinese Medicine. It is an important plant
with several medicinal, and nutritional values used in Asian and Chinese Tradition
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medicine. Ginger and its general compounds such as Fe, Mg, Ca,vitamin C, flavonoids,
paradols has long been used as an herbal medicine to treat various symptoms including
vomiting, pain, cold symptoms and it has been shown to have anti-inflammatory,
cardiotonic, cytotoxic. It has been widely used for arthritis, cramps, sprains, sore throats,
dementia, fever and infectious diseases. Ginger leaves have also been used for food-
the knowledge of the health benefits and sicknesses that consumers had used ginger to
treat as well as the acceptability of solar dried ginger locally. Respondents enumerated 22
and an aphrodisiac which they had used ginger to cure. More than half of respondents
(74.3%) had used ginger to cure upper respiratory infections with an almost unanimous
Jiang (2019) reported on the diverse range of health properties that they possess
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Spices and herbs such as clove, rosemary, sage, oregano, and cinnamon are excellent
sources of antioxidants with their high content of phenolic compounds. It is evident that
frequent consumption of spicy foods was also linked to a lower risk of death from cancer
and ischemic heart and respiratory system diseases. However, the actual role of spicesand
herbs in the maintenance of health, specifically with regards to protecting against the
highlights potential health benefits ofcommonly used spices and herbs such as chili
Zadeh and Kor (2014)Ginger has been used by traditional Chinese and Indian
medicine for over 25 centuries. Fresh ginger contains 80.9% moisture, 2.3% protein,
0.9% fat, 1.2% minerals, 2.4% fibre and 12.3% carbohydrates. The active components of
used to improve the flow of body fluids. It stimulates blood circulation throughout the
body by powerful stimulatory effect on the heart muscle and by diluting blood. The effect
(PGE2) production was examined after giving rats a raw aqueous extract of ginger daily
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CHAPTER III
METHODOLOGY
This chapter presents the method and procedures used in collecting and gathering
Research Design
Housefly.
Procedures
Ginger and garlic are washed thoroughly with clean water after peeling. Five
hundred (500) grams of Ginger was shredded and this shredded Ginger extracted using
cloth and it produced 170 ml of extract. An aqueous extract of Garlic was prepared by
shredding of 250 grams of garlic and adding 35 ml of water and it's extracted using
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of extract were place in separate spray bottles. The first treatment in mixture of 30 ml
(50%) of ginger extract , 15 ml (25%) of garlic extract and 15 ml (25%) of lemon extract.
garlic extract and 12 ml (20%) of lemon extract. The third treatment is 42 ml (70%) of
ginger extract 9 ml (15%) of garlic extract and 9 ml (15%) lemon extract . The last
treatment was the application of commercial pesticide. The obtained extract of ginger,
garlic and lemon was placed in a spray bottle to start the experiment.
C. Collection of pest
The housefly was collected to test the effectiveness of Ginger Extracts. The fly
was carefully caught by the researchers in their house. The flies were placed in a
container with small holes to ensure that the houseflies placed there wouldn't die.
D. Application
Four small container were used during the testing and each jar contained five (5)
housefly. Labels were placed accordingly in each container. Using spray bottle of the
Then the reactions of the housefly were observed. Every 10minutes the number of killed
housefly was recorded until 20 minutes. The dead housefly were disposed properly.
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significance among the effects of the different concentrations of Ginger extract and
commercial pesticide in killing housefly. T-test was also used to determine the
CHAPTER IV
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Table 1 shows the mortality of mosquito larvae after the trial, observation of perios and
four different treatments were conducted after 20 minutes observation period. Treatment
1, shows that the number of housefly killed using (50%) concentration is 1.67 .
Treatment 2 (60%) recorded a mean of 0.67 while treatment 3 (70%) recorded a mean
of 0.33 Treatment 1 has recorded the highest numbrer of killed housefly after the first
T3 10 10 10 10
T4 50 50 50 50
Table 2 shows the mean percent mortality of housefly treated with vsried concentration
of Ginger extracts after 20 minutes observation period. Treatment 4 (Pesticide) gained the
highest
mortality rate which is 50%. Among the concentration of Ginger extracts Treatment 1
(50%) recorder the highest mortality rate with 16.67%, followed by Treatment 2 (60%)
CHAPTER V
CONCLUSIONS AND RECOMMENDATIONS
Conclusions
Based from the gathered data, the researchers led to the following conclusions:
1.Ginger extracts has a potential as housefly repellent. Treatment 1 which uses 50%
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3.There is significant difference between the effects of Ginger extract and commercial
Recommendations
With the above findings of the researchers, the following recommendations ar hereby
endorsed:
4. Make people who are infested by flies every harvesting season in poultry more aware
of how the flies cause pain to everyone so that they can make a way to kill them.
5. Give information to the people that we can create way to exterminate the Houseflies.
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