1.

Copy number of genes can be determined by

a. Southern bolting
b. Western blotting
c. PCR
d. All of the above

2. Which of the following technique is most suitable for detecting the presence of gene
product
a. Southern blotting
b. Western blotting
c. PCR
d. Northern blotting

3. If a mutation occurs in an intergenic region it will be_______________

a. silent
b. lethal
c. no visible effect on cell
d. both a and c

4. Cry III proteins are generally toxic to_______________

a. lepidoptera
b. dipetra
c. lepidoptera and diptera
d. coleopteran

5. The place in between beads or nucleosomes in chromosomes where DNA is straight are
termed as________________
a. spacer
b. linkers
c. both of these
d. none of these

6. The particular array of chromosomes that individual possess is called

its_______________
a. genotype
b. karyotype
c. phenotype
d. all of the above

7. On a growing DNA strand replication always proceed on _______________

a. 3’-5’ direction
b. 5’-3’ direction
c. medially
d. non- directionally

8. F. Sanger described the complete sequence of amino acids of _______________

 a. adrenaline
b. insulin
c. glucagon
d. thyroxine

9. The site of protein synthesis in the cell is____________________

a. Golgi complex
b. endoplasmic reticulum
c. ribosomes
d. nucleus

10. Evolution is a Latin word meaning _______________

a. rolling
b. un-rolling
c. changing
d. unchanging

11. Blood grouping in man is a hereditary character which is controlled by a set of

_______
a. three genes
b. three multiple alleles
c. two genes
d. two alleles

12. First restriction enzyme was isolated in 1970 by____________

a. Morgan
b. Johanson
c. Hershey and Chase
d. H.O. Smith

13. A sequence reading GAATTC is recognized by the restriction enzyme ____________

a. EcoR1
b. EcoR2
c. Eco R3
d. none of the above

14. What sort of characters is useful in constructing phylogenetic trees?____________

a. homologous traits
b. analogous traits
c. shared derived traits
d. a and c

15. Recombinant DNA technology is used to produce bacteria that reproduce in large vats
called __________
a. thermocyler
b. bioreactor
 c. variants
d. both b and c

16. In order to insert a gene into a cell in vivo gene therapy, the fatty acid vesicles used are
known as __________
a. Vectors
b. liposomes
c. lysosomes
d. animal vector