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NRT/KS/19/2090

Bachelor of Science (B.Sc.) Semester—III Examination

BIOCHEMISTRY (Biophysical Techniques—I)
Optional Paper—II
Time : Three Hours] [Maximum Marks : 50
Note :— (1) All questions are compulsory.
(2) All questions carry equal marks.
1. Explain in detail the detectors used in UV and Visible spectrophotometry. 10
OR
(a) Describe the concept of orbital theory and electromagnetic radiations. 5
(b) Describe the applications of UV-VIS spectrophotometry. 5
2. Describe in detail the principle, instrumentation and applications of spectrofluorometry. 10
OR
Describe briefly :
(a) Titration curve of amino acid. 5
(b) Biochemically and physiologically important buffers. 5
3. Describe the principle and technique of gel filtration chromatography. 10
OR
Describe in detail paper chromatography. 10
4. Give a detailed account of affinity chromatography. 10
OR
Describe ion-exchange chromatography in detail. 10
5. Solve any TEN of the following :—
(i) Define molar extinction coefficient. 1
(ii) Define Beer’s Law ? 1
(iii) What are chromophores ? 1
(iv) What is a buffer ? 1
(v) Write the Henders on — Hasselbalch equation. 1
(vi) What is meant by isoelectric pH ? 1
(vii) Define partition coefficient. 1
(viii) Name any one adsorbent used in TLC. 1
(ix) What is meant by Void volume ? 1
(x) What is the full form of HPLC ? 1
(xi) What is the use of guard column in HPLC ? 1
(xii) Name any one gas used as mobile phase in gas chromatography. 1

CLS—13382