7 REGULATION OF GENE EXPRESSION1

1. Which of the following is not composition of Operon ():

A. promoter
B. regulatory sequence
C. operator
D. structural gene
E. trans-acting factor

2. Which of the following about gene expression concept is not correct?

A. All the gene expression always experience transcription and translation
B. some gene expression experience transcription and translation
C. some gene expression product are proteins
D. some gene expression product are not proteins
E. some gene expression product are
F. RNA

3. cis-acting elements refers to :

A. regulatory protein
B. regulatory RNA sequence
C. DNA sequence ambilateral structral gene
D. DNA sequence code for amino acid
E. DNA only at 5'end of structural gene

4. The DNA sequence that have transcriptional activation for its own gene are:
A. cis-acting elements
B. trans-acting elements
C. cis-acting factors
D. trans-acting factors
E. cis-transacting elements

5. The number of structural gene of lac operon in E.Coli is:

A. 1
B. 2
C. 3
D. 4
E. 5

6. An operon always be composed of ( ):

 A. one promoter and one coding gene
B. one promoter and several coding genes
C. several promoters and one coding gene
D. several promoters and several coding genes
E. two promoters and several coding genes

7. Trans-acting factors refer to :

A. regulatory protein with mobilizing function
B. regulatory protein with inhibiting function
C. regulatory protein with mobilizing function for its own gene
D. regulatory protein with inhibiting function for its own gene
E. regulatory protein with regulating function for another gene
8. The transcription factors which are code by special gene and will interact with
the cis-acting elements to activate another genes. Therefore, these
transcription factors refer to()
A. cis-acting elements
B. trans-acting elements
C. cis-acting factors
D. trans-acting factors
E. cis-transacting elements
9. Housekeeping genes refer to :
A. Some genes are continuouslyexpressed in almost all of the organism
B. Some genes demonstrate higher expression level once being activated
by inducer
C. some genes fluctuate in each stage of life
D. some genes code for special protein
E. fluctuate in response to enviroment change

10. Which of the following lac operon site will be binded by Lac repressor protein ?()

A. P sequence
B. 0 sequence
C. CAP bingding cite
D. | gene
E. Z gene

11. Which of the following transcription factors could recognize and bind TATA box?()
 A. TFIIA
B. TFIIB
C. TFIID
D. TFIIE
E. TFIIF

12. The general regulatory point of gene expression is ()

A. gene activation
B. transcription initiation
C. post-transcriptional processing
D. translation initiation
E. post-translational processing

13. Operator sequence is: ()

A. inducer binding site

B. ofactor binding site
C. DDRP binding site
D. repressor protein binding site
E. cAMP-CAP complex binding site

14. In the Lac operon of E.coli,cAMP-CAP binding site is at:()

A. promoter
B. structural gene
C. operator
D. attenuater(衰减子)
E. upstream of promoter

15. The gene regluation mechanism for majority prokaryotic is:()

 A. operon
B. promoter
C. enhancer
D. silencer
E. aterencer
16. The direct inducer of lac operon is:()

A. B-galactosidase
B. glactoside permease
C. glucose
D. β-galactose
E. CAP
17. The essencial control point of the majority gene expression regulation is:()

A. replication initiation
B. trannscription initiation
C. translation initiation
D. post-transcriptional regulation
E. post-translation processing

18. Which of the following have negative regulatory function in eukaryotic

transcription?()

A. operon
B. promoter
C. enhancer
D. silencer
E. aterencer

19. Which of the following have positive regulatory function in eukaryotic transcription
initiation?()
 A. operon
B. promoter
C. enhancer
D. silencer
E. aterencer
20. Where do the RNA-pol bind first in the operon during transcription initiation?()

A. structural genes
B. promoter
C. operators(0 sequence)
D. l gene
E. CAP site
21. During eukaryotic genetic transcription, RNA- pol binding:()
A. operon
B. promoter
C. enhancer
D. silencer
E. aterencer

22. The end product of gene expression could be:

A. protein
B. polypeptide chain
C. nucleic acid
D. regulatory gene
E. RNA
23. An operon always consist of: ()
A. structural genes
B. promoter
C. Operators (0 sequence)
D. regulatory sequence
E. enhancer

24. An cis-acting element always consist of:()

A. promoter
B. operon
 C. inhancer
D. silencer
E. trans-acting factor

25. The typical promoter in eukaryote consiste of:

A. TATA box
B. CAAT box
C. GC box
D. zine finger
E. leucine zipper

26. Gene expression refers to :()

A. cell mitosis
B. genetic information on DNA to guide protein synthesis
C. DNA transcription to produce RNA
D. transcription and translation
E. post-transcriptional processing

27. Which of the following about trans-acting factors are correct?()

A. have many kinds
B. are protein
C. could bind special cis-acting elements
D. exist in plasm
E. only activate the gene expression

28. Enhancer would:()

A. influence promoter over long distanc
B. influence promoter nearby
C. effect no orientation
D. effect alone
E. in the intron

29. Which of the following belong to the product of gene expression?()

A. DNA
B. tRNA
C. mRNA
D. rRNA
E. protein
08.1 SIGNAL TRANSDUCTION 1
1. Which of the following hormone acts through intracellular receptor:()
 A. adrenalin
B. adrenal cortex hormone
C. insulin
D. ACTH
E. TSH

2. Which of the following does not belong to second messenger? ()

A. cAMP
B. cGMP
C. PIP2
D. IP3
E. DAG

3. Which of the following about properties of binding of hormone and

receptor is not correct? ()

A. highly specificity
B. highly affinity
C. covalent bonding
D. reversible binding
E. certain interaction mode

4. The chemical nature of most membrane receptors is()

A. glycolipid
B. phospholipid
C. lipoprotein
D. glycoprotein
E. steroid
5. Small molecules which are responsible for intracellular signal
transduction are called()?

A. receptor
B. vetor
C. transmitter
D. first messenger
E. second messenger

6. Which of the following do not belongs to second messenger? ()

A. cAMP
B. Ca2+
 C. cGMP
D. IP3
E. Ach

7. The a-subunit of G protein have the activity of:

A. GTPase
B. ATPase
C. TTPase
D. CTPase
E. UTPase

8. G protein is referred to:()

A. PKA
B. guanylate cyclase
C. PKG
D. GRB2 binding protein
E. Guanylate binding protein

9. Which of the following is G protein coupled receptor? ()

A. cyclic receptor
B. 7-helices transmembrane receptor
C. catalytic receptor
D. nucleic receptor
E. cytosol receptor

10. The chemical nature of intracellular receptors is()

A. DNA bind protein

B. G protein
C. glycoprotein
D. lipoprotein
E. glycolipid

11. Properties of binding of H and R are:()

A. highly specificity
B. highly affinity
C. covalent bonding
D. saturation
 E. reversible binding

12. Which of the following hormone act through membrane receptor? ()

A. adrenalin
B. thyroxin
C. growth factor
D. insulin
E. estrin

08.2 SIGNAL TRANSDUCTION 2

1. Which of the following does not participate in AC-cAMP-PKA pathway
()

A. receptor
B. AC
C. hormon
D. G Protein
E. cGMP

2. Which of the following enzyme could be influenced by activated G

protein directly?()

A. PKA
B. PKC
C. PKG
D. PLA
E. PLC

3. The insulin receptor have ( ) activity:()

A. PKA
B. PKC
C. PKG
D. PTK
E. Ca2+- CaM kinase

4. Activated PKC could phosphorylate the () amino acid residue of protein.

()

A. Tyr/Ser
 B. Tyr/Thr
C. Ser/ThrD.Ser/His
D. Thr/His
5. cAMP-dependent protein kinase is()

A. receptor-PTK
B. non-receptor-PTK
C. PKC
D. PKA
E. PKG

6. cAMP could activate ()

A. PKA
B. PKC
C. PKG
D. PTK
E. Ca2+- CaM kinase

7. IP3 could:()
A. advance the generation of DAG
B. increase the activity of embrane Ca2+ pump
C. advance the Ca2+release from the ER to plasm
D. advance Ca2+ bind CaM
E. advance Ca2+ bind PKC

8.cGMP could activate ()

A. PKA
B. PKC
C. PKG
D. PTK
E. Ca2+- CaM kinase

9.Which of the following do not participate in Cellular Signal

Transduction?()
A. NO
B. inslulin
C. Ach
D. Ca2+
E. NAD+
10. Which of the following do not belong to extrocellular message?()

A. prostaglandin
B. adrenalin
C. PIP3
D. noradrenalin
E. growth factor

11.DAG could activate()

A. PKA
B. PKC
C. PKG
D. PTK
E. Ca2+- CaM dependant kinase

12.Which of the following could activate PKC directly()

A. cAMP
B. DAG
C. IP3
D. PIP2
E. cGMP

13.Which of the following could make endocytoplasmic reticulum() and

sarcoplasmic reticulum() release Ca2+ into plasm()

A. cAMP
B. cGMP
C. IP3
D. Ca2+
E. DAG
14. The allosteric activator of PKA is:()

A. cAMP
B. cGMP
C. ATP
D. GTP
E. ADP
15. DAG-PKC pathway consist of: ()

A. Ca2+
B. DAG
C. IP3
 D. PKC
E. phosphatidy| serine

16. Which of the following do not participate in the pathway of

intracellular receptor? ()

A. intracellular receptor
B. G protein
C. AC
D. hormon response element (HRE)
E. PLC

17. PLC can catalyzed PIP2 to hydrolyze and produce: ()

A. cAMP
B. cGMP
C. DAG
D. IP3
E. TAG

18. Which of the following could influence the content of cAMP in the
cell?

A. PKA
B. ATPase
C. phosphodiesterase
D. AC
E. Phospholipase
10.1 DNA TECHNOLOGY
1. The enzyme can recognize special sequences and cleave DNA at these
specific base sequences is called? ()

A. Restriction endonuclease
B. Restriction extronuclease
C. non-Restriction endonuclease
D. non-restriction extronuclease
E. Dnase

2. A certain restricion endonuclease cut DNA by means of

GGG▼CGCCC,and will generate( ) salient(突出的) sticky end:()

A. 1 nucleotide
B. 2 nucleotides
C. nucleotides
D. 4 nucleotides
E. 5 nucleotides

3. The plasmid used in DNA recombination techology is: ()

A. Part of the cell chromosomal DNA

B. the independent hereditary unit outside of the becterial
genomic DNA
C. part of the viral genomic DNA
D. part of the eukaryotic chromosome genomic DNA
E. the independent hereditary unit outside of eukaryotic
chromosomal DNA

4. Which of the following is the most general characteristic of the vector

needed in DNA cloning?()

A. Ampicillin resistance
 B. kanamycin resistance
C. self replication ability
D. self transcription ability
E. self exprssion ability

5. The optimal cell system to express human protein is()

A. E.coli expression system

B. prokaryotic expression system
C. yeast cell expression system
D. insect expression system
E. mammalia cell expression system

6. The procedure of genetic engeneering could be summerized to be()

A. Isolation of target gene, linerization and identification

B. seperation, cutting,ligation, transformation, screening
C. Transformation of target gene into receptor cell and Screening for
recombinant plasmids
D. Ligation of target DNA and vector
E. none of the above

7. Which of the following is the end that T4 ligase catalize to link

together?()

A. 5'-OH and 3'-OH

B. 5'-0H and 3'-P
C. 5'-P and 3'-OH
D. 5'-P and 3'-P
E. any two end of the above
8. The process of DNA cloning does not include()

A. Isolation of target gene

B. Selection and construction of vectors
C. Screening for recombinant plasmids
D. Transformation of target gene into receptor cell
E. probe synthesis for target gene detection

9. The process of DNA cloning does not include()

A. Isolation of target gene

B. purification of target protein
C. Ligation of target DNA and vector
D. ransformation of target gene into receptor cell
E. Screening for recombinant plasmids