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5 authors, including:
Afam-Ezeaku Chikaodili
Nnamdi Azikiwe University, Awka
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Authors’ contributions
This work was carried out in collaboration among all authors. Author ECF designed the study,
performed the statistical analysis, wrote the protocol, and wrote the first draft of the manuscript.
Authors NNE and OOJ managed the analyses of the study. Authors MOA and AECE managed
the literature searches. All authors read and approved the final manuscript.
Article Information
DOI: 10.56557/JACSI/2023/v14i28354
Received: 11/04/2023
Original Research Article Accepted: 13/06/2023
Published: 25/08/2023
ABSTRACT
The use of coconut oil as a moisturiser in cream manufacture has been a recent topic of discussion.
In this work, coconut oil was extracted from coconut seeds using the Soxhlet extraction method,
and the resulting oil was then characterised and utilised to make cream. As a solvent, regular
hexane was employed during the extraction procedure. The coconut seeds' poximate composition
reveals that their moisture content ranges from 38 to 48 percent, along with ash (1 to 4 percent),
lipids (18 to 20 percent), protein (6 to 7 percent), fibre (3 to 10 percent), and carbs (15 to 22
percent). According to the analysis's findings, coconut seed oil has a yield percentage of 20.11%, a
pH of 5.03, a refractive index of 0.81, and a specific gravity of 0.97. 208.61 mg KOH/g of
saponification value, Acid value of 4.207mgKOH, peroxide value of 33.20mgKOH/kg, and iodine
value of 12.54gI/100g. Using the fundamental ingredients of moisturizer(s), emulsifier system,
water, and preservative, body moisturising cream was created in the lab. The percentage yield,
colour, specific gravity, and pH of the created cream were assessed and compared to commercial
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J. Appl. Chem. Sci. Int., vol. 14, no. 2, pp. 18-27, 2023
Frances et al.; J. Appl. Chem. Sci. Int., vol. 14, no. 2, pp. 18-27, 2023; Article no.JACSI.11548
cream. Evaluation revealed that the manufactured cream met requirements and had acceptable
moisturization. The body lotion made from coconut oil had a constant texture, showed little patch
reactivity, and smelled well.
Conclusion: Since coconut seed oil cream has superior quality than commercial body lotion, it can
be utilised for cosmetic purposes.
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restricted for a variety of reasons, including lipid physiochemical properties of the extracted oil
oxidation and microbial contamination caused by using standard AOAC method of 2005 in Awka
the high temperature and unclean conditions metropolis [12].
during drying and storage (Kaunitz, and Dayrit.
2012). Copra does, however, contain proteins of 2. MATERIALS AND METHODS
a pretty excellent nutritional grade. The recovery
of coconut oil via the conventional wet method is 2.1 Apparatus/Materials
typically poor, at approximately 30%, in an effort
to meet the general need for oil production from Density bottle, digital weighing balance
coconuts, support the country's struggle to foster viscometer, refractometer, PH electrode,
indigenous technology, and try to save our Refractometer, Heating mantle, thermometer,
foreign exchange [11]. As a result, it is necessary water bath volumetric flask reflux condenser,
to investigate several techniques that are 3 3
burette (50cm ), pipette (250cm ) separating
currently used to produce coconut oil from either funnel, measuring cylinder, round bottom flask,
fresh coconut meat or copra (dried coconut glass rod /stirrer, beakers, conical flask, spatula,
kernel). These technologies include the wet Distillation apparatus, mechanical stirrer,
process, the dry process, and the solvent beakers.
extraction all of which are more sanitary than the
standard extraction procedures. 2.2 Chemical Reagents
1.2 Aim and Objectives of the Study Chloroform, Diethyl ether, ethanol, acetic acid,
The aim of this study is to extract and potassium iodide, sodium hydroxide, sodium
characterize coconut oil in cream production from thiosulphate, alcoholic potassium hydroxides,
coconuts sold in the open market within Awka HCL, Distilled water, phanolphthalin indicator.
metropolis
2.3 Sample Collection
1.3 Objectives
The coconut seed samples were purchased from
The specifc objectives includes to: Extract oil the Eke Awka market in Anambra state in
from the kernel of coconut using indirect Nigeria. The seed were hulled with a stainless
o
extraction method, characterization of each oil for knife, and dried at 40 C in the oven for 5 hours.
its physiochemical properties and utilization of Two hundred gram of the seed was ground into
the coconut oil in cream production. small pieces using a mains- operated food
grinder (HR 2811 Philip Model). The dried
1.4 Significance of the Study samples were kept in an air tight sample
o
container in a refrigerator (4 C) until ready for
There are various implications when describing analysis.
coconut oil's versatility. Communities in the West
African nations rely heavily on the money
2.4 Experimental Procedure
generated by agribusiness. In order to fulfil
market demand and the needs of society at
2.4.1 Extraction of oil from coconut oil
large, this research will allow researchers to
boost the yield of coconut oil. The findings will
In this procedure, the Soxhlet extractor was
also serve as a database for future industrialist
employed for the indirect extraction (leaching). A
who want to start making oil from coconut seed.
thimble containing 10g of ground coconut
The work's findings will also help the
samples was put inside the inner tube of the
unemployed in society since they will highlight
soxhlet extractor. Then, this equipment was
important and novel uses for coconut oil,
attached to a flask with a circular bottom that
particularly in the cosmetics industry. The
held 200 millilitres of the solvent (n-hexane or).
government or private individuals can also set up
On the device, a reflux condenser was also
new industries to produce coconut oil-based
placed and attached. A retort stand was used to
goods, which will boost their income.
secure the set-up, and a heating mantle was
1.5 Scope of the Study turned on for extraction durations of 30, 60, 90,
and 120 minutes at the solvent's boiling point
The present work covers the extractinon and (600C). The solvent-condensed vapour that was
characterization of coconut oil to determine the condensed by the condenser and passed
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Frances et al.; J. Appl. Chem. Sci. Int., vol. 14, no. 2, pp. 18-27, 2023; Article no.JACSI.11548
through the tube slowly fills the thimble as it is into the liquid sample and applying suction
received. The part of the sample that had been pressure until it reached the upper timing mark of
extracted in the thimble was removed when the the viscometer, the viscosity metre was charged
solvent reached the top of the tube and with the sample. Then, it was rotated back to its
syphoned over into the flask. The device was regular vertical position. The sample was given
disassembled and the procedure automatically around 10 minutes to reach the bath temperature
o
repeated itself for the extraction times. of 29 C before inserting the viscometer into a
holder and into the constant temperature bath.
After extraction and drying in an electric oven, The sample was then drawn just above the top
the bulk of coconut was weighed, and the weight time mark using the suction force that had been
was noted. The same Soxhlet extractor was used applied to the thinner arm. The sample defect
for the solvent recovery procedure. The miscella, was timed as it freely flowed from the top timing
also known as the solvent and oil combination, mark to the lower timing mark, and the afflux time
was heated in the flask. The solvent vaporised was then recorded.
under continuous heating and then condensed in
the thimble chamber. Earlier than being 2.4.5 Determination of refractive index
syphoned back into the flask, the solvent was
collected. After that, the oil was retrieved, and This determination was made using a refractive
the mass was noted. metre. A few drops of the sample were placed in
the refractometer's glass slide. To maintain a
2.4.2 Characterization of the extracted consistent temperature on the glass slide, water
coconut oil heated to 30°C was pumped around it. The black
area seen through the refract meter's eyepiece
The following physicochemical characteristics was adjusted to be parallel to the cross'
were analyzed in the oil, Specific gravity, intersection with no parallax error. The refractive
Refractive index, PH, Viscosity, Acid value, index was the target of the scale's pointer.
Saponification value, Iodine value, Ester value, Refractive index was determined by repeating
Free fatty acid value, Peroxide value. this process and recording the mean value.
Density bottle was used in determining the 2g of the oil sample was put to a clean, dry 25ml
density of the oil. A clean and dry specific gravity beaker, which was then slowly swirled after
bottle was weighed as (W 0) and then filled with receiving 13ml of hot, distilled water. Then, it was
o
the oil and reweighed to give (W 1). The oil was chilled to 25 C in a cold-water bath. The PH
substituted with water after washing and drying electrode was calibrated with butter solution,
the bottle and weighed to give (W 2). The formular submerged in the sample, and its reading and
for specific gravity is recording of the PH value.
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The indicator approach was applied in With the use of Pearson, (1970) titrametric
accordance with 1S0 3657 (1988). Weighing 2g approach, the peroxide value was calculated. A
of the oil sample into a conical flask. Then 2.5ml conical flask containing 1g of the oil sample and
of potassium hydroxide 0.1N ethanolic was 20ml of a solvent mixture (Glacial acetic acid and
added. The continually stirred mixture was given chloroform) were weighed. This spent 30
60 minutes to gradually boil. The flask holding seconds in the hot water. The liquid was then
the mixture had a reflux condenser on it. Warm transferred into a flask that already had 20ml of
solution was diluted with a few drops of 5% iodine solution inside. Using starch as an
phenolpthalain indicator before being titrated with indicator, 0.002m sodium thiosulphate solution
0.5n HC to the end point when the indicator's was then titrated in the flask after being cleaned
pink colour just about vanished. For additional up with 25ml of distilled water. Along with the oil
samples and a blank, the same process was samples, a blank was also created.
applied. The saponification value formula is as The following formula provides the peroxide
follows: value:
–
–
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Frances et al.; J. Appl. Chem. Sci. Int., vol. 14, no. 2, pp. 18-27, 2023; Article no.JACSI.11548
emulsion will be allowed to gently cool while total acid value, percentage of free fatty acid,
being stirred until it reaches 35°C. The emulsion saponification value, and mechanical testing
will next be mixed with the scent that has employing Sherman (2001)'s techniques.
previously been partially dissolved in glycerol.
Until homogeneity is attained, the emulsion is 2.6 Statistics Analysis
agitated.
Using the Statistical Package for Social Sciences
To improve homogeneity, the emulsion will be (SPSS version 17.0), two-way analysis of
covered and left for 12 hours before being variance (ANOVA) was performed on the
combined once more quickly. A appropriate analysis's output data. At p0.05, there was a
plastic container with a screw cover will then be significant difference between the averages.
used to pour the emulsion into for storage and
examination. 3. RESULTS
2.5.4 Quality control analysis The result of the physical and chemical
characterization of the coconut oil extracted is
Stability tests and cosmetic control analysis were given in Table 1. Based on the results of the
performed on the three created emulsions as proximate composition of the coconut seeds as
well as the moisturising cream that was shown in Table 1, moisture content ranges from
purchased. These tests include those that 38.00 to 48.00%, ash (1.00 to 4.00%), fats (18.59
measure pH, conductivity, microscopic to 20.88%), protein (6.20 to 7.93%), fiber (3.00 to
inspection, cyclical temperature fluctuation tests, 10.00%) and carbohydrates (15.91 to 22.21%).
Samples Moisture (%) Ash (%) Fat/oil (%) Fiber (%) Protein (%) Carbohydrate (%)
COCONUT 1 48.00 + 0.11 1.00+ 0.31 18.59+ 0.10 4.00+ 0.40 6.20+ 0.21 22.21+ 0.11
COCONUT 2 47.80+ 1.01 4.00+ 0.21 20.86+ 0.00 3.50+ 0.01 7.93+ 0.30 15.91+ 0.20
COCONUT 3 38.00+ 0.01 3.00+ 0.01 20.88+ 0.11 10.00+ 0.21 7.80+ 0.00 20.32+ 0.21
50
45
40
35
30
25
COCONUT 1
20
COCONUT 2
15 COCONUT 3
10
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20.11
20
15
8.415
10
5.03
5 2.54
0.018 0.4207
COCONUT OIL
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Table 4. Evaluation data of Shea butter seed oil cream with carol white body lotion
100
90
80
70
60
coconut oil cream
50 Smooth
Carol white lotion
40 Smooth
30
20
10
0
Specific % Moisture pH
gravity
Fig. 3. Evaluation data of shea butter seed oil cream with carol white body lotion
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seed oil, the oil will have a lower amount of With its inherent antibacterial characteristics,
unsaturated fatty acids. However, because the coconut seed aids in skin cleansing and includes
oil's iodine value was within the acceptable range natural compounds that moisturize and
and less than 100 grammes of fat, it could be rejuvenate skin cells. Produced coconut seed
categorized as non-drying oil (Grimwood et al., cream has no artificial perfumes and is
2015). The coconut oil cream was created environmentally friendly. This product is
utilising a straightforward laboratory procedure especially designed for persons with extremely
[13-15]. dry skin, itchy skin brought on by skin allergies
and irritation, burn victims, psoriasis sufferers,
Additionally, the oil was classified as a non- sunburn victims, and people with skin diseases
drying oil with an iodine number of 13.69g including eczema.
I2/100g, suggesting a high content of
unsaturated fatty acids that can undergo radical 5. CONCLUSION
chain polymerization. So, compared to avocado
seed oil, the oil will have a lower amount of The outcome strongly implies that coconut oil
unsaturated fatty acids. However, because the may be a contender in the cosmetic industry,
oil's iodine value complies with the norm and has particularly for use as moisturisers in body cream
less than 100 fat, it may be categorised as non- manufacture. Coconut is a medicinal plant that
drying oil (Cha et al., 2020). 13 mg/kg of has a wide range of medical, nutraceutical, and
peroxide was obtained in the oil. The oil's other purposes. As a result, it is in high demand
susceptibility to oxidative rancidity is determined worldwide. The South Eastern states as a whole,
in part by the peroxide value. The value including Awka and Onitsha, are the main
discovered by the research fell short of what markets for coconut oil and its extracts. Among
Giammattei et al. (2019) reported. This the different therapeutic plants, coconut provides
demonstrated that the oil will not be prone to a great economic possibility due to the rapidly
rancidity readily. expanding demand for it on the global market. All
cosmetic items should contain this oil, and the
A coconut oil-based body cream is a topical National Agency for Food and Drug
product with a low to heavy viscosity that is Administration and Control (NAFDAC) should
meant to be applied to healthy skin. With clean check to identify which businesses are still
hands, a brush, a clean cloth, cotton wool, or manufacturing body cream with hydroquinone
gauze, it can be applied to exposed skin. Many and strongly urge them to switch to coconut seed
creams, particularly body creams, are designed oil.
to moisturise, soften, and smooth the skin rather
than to carry medications. These are very well- 6. RECOMMENDATION
liked among older and older people. When used Due to the therapeutic effectiveness of body
on the face, they are sometimes categorised as lotion, this study has demonstrated that people
cosmetics and may include perfumes. do not necessarily need to apply it only to
moisturise their skin. Therefore, it is advised that
The majority of body creams are oil-in-water coconut body cream be used in every home for
emulsions that are kept together by a chemical appropriate skincare.
like cetearyl alcohol, although water-in-oil lotions
can also be created. The aqueous and oily Second, both the government and people should
phases, an emulgent to prevent the separation of promote the planting of this seed. Government
these two phases, and, if employed, the drug should establish rigorous routines to monitor the
ingredient or substances, are the essential oils and make sure that people are consuming
components of a skin care lotion, cream, or gel vegetable oils that are safe for their health. To
emulsion (that is, combinations of oil and water). promote human health, people should be
Lotions frequently contain a wide range of encouraged to conduct study on this plant,
additional substances, including scents, glycerol, particularly on its antiseptic, antipyretic,
petroleum jelly, colours, preservatives, proteins, antihypertensive, antiepileptic, antifungal, and
and stabilizing agents. antibacterial qualities.
Our body creams are made with coconut oil, COMPETING INTERESTS
which is well-known for its effectiveness as a
cooling agent and is particularly useful for Authors have declared that no competing
relieving sunburn, chapped skin, and dry skin. interests exist.
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